7VH6

Cryo-EM structure of the hexameric plasma membrane H+-ATPase in the active state (pH 6.0, BeF3-, conformation 1, C1 symmetry)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0005215	molecular_function	transporter activity
A	0005524	molecular_function	ATP binding
A	0005739	cellular_component	mitochondrion
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0008553	molecular_function	P-type proton-exporting transporter activity
A	0015662	molecular_function	P-type ion transporter activity
A	0016020	cellular_component	membrane
A	0016887	molecular_function	ATP hydrolysis activity
A	0019829	molecular_function	ATPase-coupled monoatomic cation transmembrane transporter activity
A	0022890	molecular_function	inorganic cation transmembrane transporter activity
A	0032126	cellular_component	eisosome
A	0045121	cellular_component	membrane raft
A	0046872	molecular_function	metal ion binding
A	0051453	biological_process	regulation of intracellular pH
A	0055085	biological_process	transmembrane transport
A	0098662	biological_process	inorganic cation transmembrane transport
A	0120029	biological_process	proton export across plasma membrane
A	1902600	biological_process	proton transmembrane transport
A	1902906	biological_process	proteasome storage granule assembly
A	1904263	biological_process	positive regulation of TORC1 signaling
B	0000166	molecular_function	nucleotide binding
B	0005215	molecular_function	transporter activity
B	0005524	molecular_function	ATP binding
B	0005739	cellular_component	mitochondrion
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0008553	molecular_function	P-type proton-exporting transporter activity
B	0015662	molecular_function	P-type ion transporter activity
B	0016020	cellular_component	membrane
B	0016887	molecular_function	ATP hydrolysis activity
B	0019829	molecular_function	ATPase-coupled monoatomic cation transmembrane transporter activity
B	0022890	molecular_function	inorganic cation transmembrane transporter activity
B	0032126	cellular_component	eisosome
B	0045121	cellular_component	membrane raft
B	0046872	molecular_function	metal ion binding
B	0051453	biological_process	regulation of intracellular pH
B	0055085	biological_process	transmembrane transport
B	0098662	biological_process	inorganic cation transmembrane transport
B	0120029	biological_process	proton export across plasma membrane
B	1902600	biological_process	proton transmembrane transport
B	1902906	biological_process	proteasome storage granule assembly
B	1904263	biological_process	positive regulation of TORC1 signaling
C	0000166	molecular_function	nucleotide binding
C	0005215	molecular_function	transporter activity
C	0005524	molecular_function	ATP binding
C	0005739	cellular_component	mitochondrion
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0008553	molecular_function	P-type proton-exporting transporter activity
C	0015662	molecular_function	P-type ion transporter activity
C	0016020	cellular_component	membrane
C	0016887	molecular_function	ATP hydrolysis activity
C	0019829	molecular_function	ATPase-coupled monoatomic cation transmembrane transporter activity
C	0022890	molecular_function	inorganic cation transmembrane transporter activity
C	0032126	cellular_component	eisosome
C	0045121	cellular_component	membrane raft
C	0046872	molecular_function	metal ion binding
C	0051453	biological_process	regulation of intracellular pH
C	0055085	biological_process	transmembrane transport
C	0098662	biological_process	inorganic cation transmembrane transport
C	0120029	biological_process	proton export across plasma membrane
C	1902600	biological_process	proton transmembrane transport
C	1902906	biological_process	proteasome storage granule assembly
C	1904263	biological_process	positive regulation of TORC1 signaling
D	0000166	molecular_function	nucleotide binding
D	0005215	molecular_function	transporter activity
D	0005524	molecular_function	ATP binding
D	0005739	cellular_component	mitochondrion
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0008553	molecular_function	P-type proton-exporting transporter activity
D	0015662	molecular_function	P-type ion transporter activity
D	0016020	cellular_component	membrane
D	0016887	molecular_function	ATP hydrolysis activity
D	0019829	molecular_function	ATPase-coupled monoatomic cation transmembrane transporter activity
D	0022890	molecular_function	inorganic cation transmembrane transporter activity
D	0032126	cellular_component	eisosome
D	0045121	cellular_component	membrane raft
D	0046872	molecular_function	metal ion binding
D	0051453	biological_process	regulation of intracellular pH
D	0055085	biological_process	transmembrane transport
D	0098662	biological_process	inorganic cation transmembrane transport
D	0120029	biological_process	proton export across plasma membrane
D	1902600	biological_process	proton transmembrane transport
D	1902906	biological_process	proteasome storage granule assembly
D	1904263	biological_process	positive regulation of TORC1 signaling
E	0000166	molecular_function	nucleotide binding
E	0005215	molecular_function	transporter activity
E	0005524	molecular_function	ATP binding
E	0005739	cellular_component	mitochondrion
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0008553	molecular_function	P-type proton-exporting transporter activity
E	0015662	molecular_function	P-type ion transporter activity
E	0016020	cellular_component	membrane
E	0016887	molecular_function	ATP hydrolysis activity
E	0019829	molecular_function	ATPase-coupled monoatomic cation transmembrane transporter activity
E	0022890	molecular_function	inorganic cation transmembrane transporter activity
E	0032126	cellular_component	eisosome
E	0045121	cellular_component	membrane raft
E	0046872	molecular_function	metal ion binding
E	0051453	biological_process	regulation of intracellular pH
E	0055085	biological_process	transmembrane transport
E	0098662	biological_process	inorganic cation transmembrane transport
E	0120029	biological_process	proton export across plasma membrane
E	1902600	biological_process	proton transmembrane transport
E	1902906	biological_process	proteasome storage granule assembly
E	1904263	biological_process	positive regulation of TORC1 signaling
F	0000166	molecular_function	nucleotide binding
F	0005215	molecular_function	transporter activity
F	0005524	molecular_function	ATP binding
F	0005739	cellular_component	mitochondrion
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0008553	molecular_function	P-type proton-exporting transporter activity
F	0015662	molecular_function	P-type ion transporter activity
F	0016020	cellular_component	membrane
F	0016887	molecular_function	ATP hydrolysis activity
F	0019829	molecular_function	ATPase-coupled monoatomic cation transmembrane transporter activity
F	0022890	molecular_function	inorganic cation transmembrane transporter activity
F	0032126	cellular_component	eisosome
F	0045121	cellular_component	membrane raft
F	0046872	molecular_function	metal ion binding
F	0051453	biological_process	regulation of intracellular pH
F	0055085	biological_process	transmembrane transport
F	0098662	biological_process	inorganic cation transmembrane transport
F	0120029	biological_process	proton export across plasma membrane
F	1902600	biological_process	proton transmembrane transport
F	1902906	biological_process	proteasome storage granule assembly
F	1904263	biological_process	positive regulation of TORC1 signaling

Functional Information from PROSITE/UniProt

site_id	PS00154
Number of Residues	7
Details	ATPASE_E1_E2 E1-E2 ATPases phosphorylation site. DKTGTLT

Chain	Residue	Details
A	ASP378-THR384

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4242
Details	TOPO_DOM: Cytoplasmic => ECO:0000255

Chain	Residue	Details
A	MET1-LYS115
B	GLU875-THR918
C	MET1-LYS115
C	GLN161-ASN291
C	VAL348-ILE719
C	THR775-GLN824
C	GLU875-THR918
D	MET1-LYS115
D	GLN161-ASN291
D	VAL348-ILE719
D	THR775-GLN824
A	GLN161-ASN291
D	GLU875-THR918
E	MET1-LYS115
E	GLN161-ASN291
E	VAL348-ILE719
E	THR775-GLN824
E	GLU875-THR918
F	MET1-LYS115
F	GLN161-ASN291
F	VAL348-ILE719
F	THR775-GLN824
A	VAL348-ILE719
F	GLU875-THR918
A	THR775-GLN824
A	GLU875-THR918
B	MET1-LYS115
B	GLN161-ASN291
B	VAL348-ILE719
B	THR775-GLN824

---

site_id	SWS_FT_FI2
Number of Residues	120
Details	TRANSMEM: Helical; Name=1 => ECO:0000255

Chain	Residue	Details
A	PHE116-ALA136
B	PHE116-ALA136
C	PHE116-ALA136
D	PHE116-ALA136
E	PHE116-ALA136
F	PHE116-ALA136

---

site_id	SWS_FT_FI3
Number of Residues	240
Details	TOPO_DOM: Extracellular => ECO:0000255

Chain	Residue	Details
A	GLY137-ASP140
C	TYR314-TYR325
C	ASP739-ARG754
C	SER846-ARG857
D	GLY137-ASP140
D	TYR314-TYR325
D	ASP739-ARG754
D	SER846-ARG857
E	GLY137-ASP140
E	TYR314-TYR325
E	ASP739-ARG754
A	TYR314-TYR325
E	SER846-ARG857
F	GLY137-ASP140
F	TYR314-TYR325
F	ASP739-ARG754
F	SER846-ARG857
A	ASP739-ARG754
A	SER846-ARG857
B	GLY137-ASP140
B	TYR314-TYR325
B	ASP739-ARG754
B	SER846-ARG857
C	GLY137-ASP140

---

site_id	SWS_FT_FI4
Number of Residues	114
Details	TRANSMEM: Helical; Name=2 => ECO:0000255

Chain	Residue	Details
A	TRP141-VAL160
B	TRP141-VAL160
C	TRP141-VAL160
D	TRP141-VAL160
E	TRP141-VAL160
F	TRP141-VAL160

---

site_id	SWS_FT_FI5
Number of Residues	126
Details	TRANSMEM: Helical; Name=3 => ECO:0000255

Chain	Residue	Details
A	GLY292-PHE313
B	GLY292-PHE313
C	GLY292-PHE313
D	GLY292-PHE313
E	GLY292-PHE313
F	GLY292-PHE313

---

site_id	SWS_FT_FI6
Number of Residues	126
Details	TRANSMEM: Helical; Name=4 => ECO:0000255

Chain	Residue	Details
A	THR326-ALA347
B	THR326-ALA347
C	THR326-ALA347
D	THR326-ALA347
E	THR326-ALA347
F	THR326-ALA347

---

site_id	SWS_FT_FI7
Number of Residues	108
Details	TRANSMEM: Helical; Name=5 => ECO:0000255

Chain	Residue	Details
A	ASP720-TYR738
B	ASP720-TYR738
C	ASP720-TYR738
D	ASP720-TYR738
E	ASP720-TYR738
F	ASP720-TYR738

---

site_id	SWS_FT_FI8
Number of Residues	114
Details	TRANSMEM: Helical; Name=6 => ECO:0000255

Chain	Residue	Details
A	LEU755-LEU774
B	LEU755-LEU774
C	LEU755-LEU774
D	LEU755-LEU774
E	LEU755-LEU774
F	LEU755-LEU774

---

site_id	SWS_FT_FI9
Number of Residues	120
Details	TRANSMEM: Helical; Name=7 => ECO:0000255

Chain	Residue	Details
A	LEU825-TRP845
B	LEU825-TRP845
C	LEU825-TRP845
D	LEU825-TRP845
E	LEU825-TRP845
F	LEU825-TRP845

---

site_id	SWS_FT_FI10
Number of Residues	96
Details	TRANSMEM: Helical; Name=8 => ECO:0000255

Chain	Residue	Details
A	VAL858-TYR874
B	VAL858-TYR874
C	VAL858-TYR874
D	VAL858-TYR874
E	VAL858-TYR874
F	VAL858-TYR874

---

site_id	SWS_FT_FI11
Number of Residues	6
Details	ACT_SITE: 4-aspartylphosphate intermediate => ECO:0000250

Chain	Residue	Details
A	ASP378
B	ASP378
C	ASP378
D	ASP378
E	ASP378
F	ASP378

---

site_id	SWS_FT_FI12
Number of Residues	12
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
A	ASP634
E	ASP638
F	ASP634
F	ASP638
A	ASP638
B	ASP634
B	ASP638
C	ASP634
C	ASP638
D	ASP634
D	ASP638
E	ASP634

---

site_id	SWS_FT_FI13
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18407956

Chain	Residue	Details
A	SER61
B	SER61
C	SER61
D	SER61
E	SER61
F	SER61

---

site_id	SWS_FT_FI14
Number of Residues	6
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:19779198

Chain	Residue	Details
A	THR175
B	THR175
C	THR175
D	THR175
E	THR175
F	THR175

---

site_id	SWS_FT_FI15
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:19779198

Chain	Residue	Details
A	SER911
B	SER911
C	SER911
D	SER911
E	SER911
F	SER911

---

site_id	SWS_FT_FI16
Number of Residues	6
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:19779198

Chain	Residue	Details
A	THR912
B	THR912
C	THR912
D	THR912
E	THR912
F	THR912

---

site_id	SWS_FT_FI17
Number of Residues	6
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:17330950

Chain	Residue	Details
A	THR918
B	THR918
C	THR918
D	THR918
E	THR918
F	THR918

---

site_id	SWS_FT_FI18
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047

Chain	Residue	Details
A	LYS252
B	LYS252
C	LYS252
D	LYS252
E	LYS252
F	LYS252

---

site_id	SWS_FT_FI19
Number of Residues	12
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:14557538

Chain	Residue	Details
E	LYS555
F	LYS555
A	LYS555
B	LYS555
C	LYS555
D	LYS555

---