7VBH
Cryo-EM structure of the GIPR/GLP-1R/GCGR triagonist peptide 20-bound human GLP-1R-Gs complex
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0002862 | biological_process | negative regulation of inflammatory response to antigenic stimulus |
| A | 0003091 | biological_process | renal water homeostasis |
| A | 0003924 | molecular_function | GTPase activity |
| A | 0003925 | molecular_function | G protein activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005525 | molecular_function | GTP binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0005834 | cellular_component | heterotrimeric G-protein complex |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0007165 | biological_process | signal transduction |
| A | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| A | 0007189 | biological_process | adenylate cyclase-activating G protein-coupled receptor signaling pathway |
| A | 0007190 | biological_process | activation of adenylate cyclase activity |
| A | 0007191 | biological_process | adenylate cyclase-activating dopamine receptor signaling pathway |
| A | 0007192 | biological_process | adenylate cyclase-activating serotonin receptor signaling pathway |
| A | 0007608 | biological_process | sensory perception of smell |
| A | 0010854 | molecular_function | adenylate cyclase regulator activity |
| A | 0010856 | molecular_function | adenylate cyclase activator activity |
| A | 0014819 | biological_process | regulation of skeletal muscle contraction |
| A | 0016020 | cellular_component | membrane |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0019001 | molecular_function | guanyl nucleotide binding |
| A | 0031683 | molecular_function | G-protein beta/gamma-subunit complex binding |
| A | 0031748 | molecular_function | D1 dopamine receptor binding |
| A | 0032588 | cellular_component | trans-Golgi network membrane |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0046907 | biological_process | intracellular transport |
| A | 0048589 | biological_process | developmental growth |
| A | 0050796 | biological_process | regulation of insulin secretion |
| A | 0050890 | biological_process | cognition |
| A | 0060348 | biological_process | bone development |
| A | 0060789 | biological_process | hair follicle placode formation |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 0070527 | biological_process | platelet aggregation |
| A | 0071377 | biological_process | cellular response to glucagon stimulus |
| A | 0071380 | biological_process | cellular response to prostaglandin E stimulus |
| A | 0071468 | biological_process | cellular response to acidic pH |
| A | 0071870 | biological_process | cellular response to catecholamine stimulus |
| A | 0071880 | biological_process | adenylate cyclase-activating adrenergic receptor signaling pathway |
| A | 0097700 | biological_process | vascular endothelial cell response to laminar fluid shear stress |
| A | 0120162 | biological_process | positive regulation of cold-induced thermogenesis |
| B | 0001750 | cellular_component | photoreceptor outer segment |
| B | 0001917 | cellular_component | photoreceptor inner segment |
| B | 0003924 | molecular_function | GTPase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005834 | cellular_component | heterotrimeric G-protein complex |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0007165 | biological_process | signal transduction |
| B | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| B | 0007200 | biological_process | phospholipase C-activating G protein-coupled receptor signaling pathway |
| B | 0007204 | biological_process | positive regulation of cytosolic calcium ion concentration |
| B | 0008283 | biological_process | cell population proliferation |
| B | 0010659 | biological_process | cardiac muscle cell apoptotic process |
| B | 0030159 | molecular_function | signaling receptor complex adaptor activity |
| B | 0030425 | cellular_component | dendrite |
| B | 0030507 | molecular_function | spectrin binding |
| B | 0042622 | cellular_component | photoreceptor outer segment membrane |
| B | 0044297 | cellular_component | cell body |
| B | 0044877 | molecular_function | protein-containing complex binding |
| B | 0045202 | cellular_component | synapse |
| B | 0047391 | molecular_function | alkylglycerophosphoethanolamine phosphodiesterase activity |
| B | 0050909 | biological_process | sensory perception of taste |
| B | 0051020 | molecular_function | GTPase binding |
| B | 0060041 | biological_process | retina development in camera-type eye |
| B | 0071456 | biological_process | cellular response to hypoxia |
| G | 0003924 | molecular_function | GTPase activity |
| G | 0005515 | molecular_function | protein binding |
| G | 0005834 | cellular_component | heterotrimeric G-protein complex |
| G | 0005886 | cellular_component | plasma membrane |
| G | 0007165 | biological_process | signal transduction |
| G | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| G | 0007191 | biological_process | adenylate cyclase-activating dopamine receptor signaling pathway |
| G | 0016020 | cellular_component | membrane |
| G | 0031681 | molecular_function | G-protein beta-subunit binding |
| G | 0071380 | biological_process | cellular response to prostaglandin E stimulus |
| G | 0071870 | biological_process | cellular response to catecholamine stimulus |
| P | 0005179 | molecular_function | hormone activity |
| P | 0005576 | cellular_component | extracellular region |
| R | 0004888 | molecular_function | transmembrane signaling receptor activity |
| R | 0004930 | molecular_function | G protein-coupled receptor activity |
| R | 0004967 | molecular_function | glucagon receptor activity |
| R | 0005515 | molecular_function | protein binding |
| R | 0005886 | cellular_component | plasma membrane |
| R | 0007165 | biological_process | signal transduction |
| R | 0007166 | biological_process | cell surface receptor signaling pathway |
| R | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| R | 0007189 | biological_process | adenylate cyclase-activating G protein-coupled receptor signaling pathway |
| R | 0007190 | biological_process | activation of adenylate cyclase activity |
| R | 0007204 | biological_process | positive regulation of cytosolic calcium ion concentration |
| R | 0007611 | biological_process | learning or memory |
| R | 0008016 | biological_process | regulation of heart contraction |
| R | 0016020 | cellular_component | membrane |
| R | 0017046 | molecular_function | peptide hormone binding |
| R | 0031204 | biological_process | post-translational protein targeting to membrane, translocation |
| R | 0038023 | molecular_function | signaling receptor activity |
| R | 0044508 | molecular_function | glucagon-like peptide 1 receptor activity |
| R | 0045776 | biological_process | negative regulation of blood pressure |
| R | 0045777 | biological_process | positive regulation of blood pressure |
| R | 0046879 | biological_process | hormone secretion |
| R | 0065008 | biological_process | regulation of biological quality |
| R | 0071377 | biological_process | cellular response to glucagon stimulus |
| R | 1990911 | biological_process | response to psychosocial stress |
Functional Information from PROSITE/UniProt
| site_id | PS00649 |
| Number of Residues | 25 |
| Details | G_PROTEIN_RECEP_F2_1 G-protein coupled receptors family 2 signature 1. CnrtFDeya.CWpdGepgsfvnvsCP |
| Chain | Residue | Details |
| R | CYS62-PRO86 |
| site_id | PS00650 |
| Number of Residues | 16 |
| Details | G_PROTEIN_RECEP_F2_2 G-protein coupled receptors family 2 signature 2. QGLMVaILYCFvNneV |
| Chain | Residue | Details |
| R | GLN394-VAL409 |
| site_id | PS00678 |
| Number of Residues | 15 |
| Details | WD_REPEATS_1 Trp-Asp (WD) repeats signature. LVSAsqDgKLIIWDS |
| Chain | Residue | Details |
| B | LEU70-SER84 | |
| B | ILE157-ILE171 | |
| B | LEU285-ALA299 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 9 |
| Details | Region: {"description":"G3 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 7 |
| Details | Region: {"description":"G4 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 7 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"P04896","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 24 |
| Details | Transmembrane: {"description":"Helical; Name=1","evidences":[{"source":"PubMed","id":"28514449","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 23 |
| Details | Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"28514449","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 25 |
| Details | Transmembrane: {"description":"Helical; Name=2","evidences":[{"source":"PubMed","id":"28514449","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 51 |
| Details | Topological domain: {"description":"Extracellular","evidences":[{"source":"PubMed","id":"28514449","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 23 |
| Details | Transmembrane: {"description":"Helical; Name=3","evidences":[{"source":"PubMed","id":"28514449","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 24 |
| Details | Transmembrane: {"description":"Helical; Name=4","evidences":[{"source":"PubMed","id":"28514449","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 22 |
| Details | Transmembrane: {"description":"Helical; Name=5","evidences":[{"source":"PubMed","id":"28514449","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI13 |
| Number of Residues | 21 |
| Details | Transmembrane: {"description":"Helical; Name=6","evidences":[{"source":"PubMed","id":"28514449","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI14 |
| Number of Residues | 20 |
| Details | Transmembrane: {"description":"Helical; Name=7","evidences":[{"source":"PubMed","id":"28514449","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI15 |
| Number of Residues | 3 |
| Details | Region: {"description":"Important for allosteric inhibitor binding","evidences":[{"source":"PubMed","id":"28514449","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI16 |
| Number of Residues | 2 |
| Details | Site: {"description":"Interaction with the endogenous ligand GLP-1","evidences":[{"source":"PubMed","id":"19861722","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI17 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"ADP-ribosylarginine","evidences":[{"source":"PubMed","id":"21901419","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI18 |
| Number of Residues | 3 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"22412906","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI19 |
| Number of Residues | 48 |
| Details | Repeat: {"description":"WD 1","evidences":[{"source":"UniProtKB","id":"P62873","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI20 |
| Number of Residues | 45 |
| Details | Repeat: {"description":"WD 2","evidences":[{"source":"UniProtKB","id":"P62873","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI21 |
| Number of Residues | 40 |
| Details | Repeat: {"description":"WD 3","evidences":[{"source":"UniProtKB","id":"P62873","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI22 |
| Number of Residues | 41 |
| Details | Repeat: {"description":"WD 4","evidences":[{"source":"UniProtKB","id":"P62873","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI23 |
| Number of Residues | 43 |
| Details | Repeat: {"description":"WD 5","evidences":[{"source":"UniProtKB","id":"P62873","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI24 |
| Number of Residues | 41 |
| Details | Repeat: {"description":"WD 6","evidences":[{"source":"UniProtKB","id":"P62873","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI25 |
| Number of Residues | 30 |
| Details | Repeat: {"description":"WD 7","evidences":[{"source":"UniProtKB","id":"P62873","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI26 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphohistidine","evidences":[{"source":"UniProtKB","id":"P62871","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
