7V5G

20S+monoUb-CyclinB1-NT (S1)

Functional Information from GO Data

Chain	GOid	namespace	contents
a	0000502	cellular_component	proteasome complex
a	0001530	molecular_function	lipopolysaccharide binding
a	0002376	biological_process	immune system process
a	0002862	biological_process	negative regulation of inflammatory response to antigenic stimulus
a	0005515	molecular_function	protein binding
a	0005634	cellular_component	nucleus
a	0005654	cellular_component	nucleoplasm
a	0005737	cellular_component	cytoplasm
a	0005813	cellular_component	centrosome
a	0005829	cellular_component	cytosol
a	0005839	cellular_component	proteasome core complex
a	0006511	biological_process	ubiquitin-dependent protein catabolic process
a	0019773	cellular_component	proteasome core complex, alpha-subunit complex
a	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
a	0051603	biological_process	proteolysis involved in protein catabolic process
a	0070062	cellular_component	extracellular exosome
A	0004298	molecular_function	threonine-type endopeptidase activity
A	0005839	cellular_component	proteasome core complex
A	0051603	biological_process	proteolysis involved in protein catabolic process
b	0000502	cellular_component	proteasome complex
b	0005515	molecular_function	protein binding
b	0005634	cellular_component	nucleus
b	0005654	cellular_component	nucleoplasm
b	0005737	cellular_component	cytoplasm
b	0005829	cellular_component	cytosol
b	0005839	cellular_component	proteasome core complex
b	0006511	biological_process	ubiquitin-dependent protein catabolic process
b	0019773	cellular_component	proteasome core complex, alpha-subunit complex
b	0031625	molecular_function	ubiquitin protein ligase binding
b	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
b	0045202	cellular_component	synapse
b	0051603	biological_process	proteolysis involved in protein catabolic process
b	0052548	biological_process	regulation of endopeptidase activity
b	0070062	cellular_component	extracellular exosome
B	0004298	molecular_function	threonine-type endopeptidase activity
B	0005839	cellular_component	proteasome core complex
B	0051603	biological_process	proteolysis involved in protein catabolic process
C	0000502	cellular_component	proteasome complex
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005839	cellular_component	proteasome core complex
C	0019774	cellular_component	proteasome core complex, beta-subunit complex
C	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0051603	biological_process	proteolysis involved in protein catabolic process
C	0070062	cellular_component	extracellular exosome
D	0000502	cellular_component	proteasome complex
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005839	cellular_component	proteasome core complex
D	0010243	biological_process	response to organonitrogen compound
D	0010498	biological_process	proteasomal protein catabolic process
D	0014070	biological_process	response to organic cyclic compound
D	0016020	cellular_component	membrane
D	0019774	cellular_component	proteasome core complex, beta-subunit complex
D	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
D	0051603	biological_process	proteolysis involved in protein catabolic process
D	0070062	cellular_component	extracellular exosome
E	0004298	molecular_function	threonine-type endopeptidase activity
E	0005839	cellular_component	proteasome core complex
E	0051603	biological_process	proteolysis involved in protein catabolic process
F	0005839	cellular_component	proteasome core complex
F	0051603	biological_process	proteolysis involved in protein catabolic process
G	0005839	cellular_component	proteasome core complex
G	0051603	biological_process	proteolysis involved in protein catabolic process
H	0004298	molecular_function	threonine-type endopeptidase activity
H	0005839	cellular_component	proteasome core complex
H	0051603	biological_process	proteolysis involved in protein catabolic process
I	0004298	molecular_function	threonine-type endopeptidase activity
I	0005839	cellular_component	proteasome core complex
I	0051603	biological_process	proteolysis involved in protein catabolic process
J	0000502	cellular_component	proteasome complex
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005654	cellular_component	nucleoplasm
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0005839	cellular_component	proteasome core complex
J	0019774	cellular_component	proteasome core complex, beta-subunit complex
J	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
J	0051603	biological_process	proteolysis involved in protein catabolic process
J	0070062	cellular_component	extracellular exosome
K	0000502	cellular_component	proteasome complex
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0005839	cellular_component	proteasome core complex
K	0010243	biological_process	response to organonitrogen compound
K	0010498	biological_process	proteasomal protein catabolic process
K	0014070	biological_process	response to organic cyclic compound
K	0016020	cellular_component	membrane
K	0019774	cellular_component	proteasome core complex, beta-subunit complex
K	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
K	0051603	biological_process	proteolysis involved in protein catabolic process
K	0070062	cellular_component	extracellular exosome
L	0004298	molecular_function	threonine-type endopeptidase activity
L	0005839	cellular_component	proteasome core complex
L	0051603	biological_process	proteolysis involved in protein catabolic process
M	0005839	cellular_component	proteasome core complex
M	0051603	biological_process	proteolysis involved in protein catabolic process
N	0005839	cellular_component	proteasome core complex
N	0051603	biological_process	proteolysis involved in protein catabolic process
O	0000502	cellular_component	proteasome complex
O	0000932	cellular_component	P-body
O	0003723	molecular_function	RNA binding
O	0004175	molecular_function	endopeptidase activity
O	0005515	molecular_function	protein binding
O	0005634	cellular_component	nucleus
O	0005654	cellular_component	nucleoplasm
O	0005737	cellular_component	cytoplasm
O	0005829	cellular_component	cytosol
O	0005839	cellular_component	proteasome core complex
O	0005840	cellular_component	ribosome
O	0006511	biological_process	ubiquitin-dependent protein catabolic process
O	0016363	cellular_component	nuclear matrix
O	0019773	cellular_component	proteasome core complex, alpha-subunit complex
O	0030016	cellular_component	myofibril
O	0030017	cellular_component	sarcomere
O	0035639	molecular_function	purine ribonucleoside triphosphate binding
O	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
O	0050727	biological_process	regulation of inflammatory response
O	0051059	molecular_function	NF-kappaB binding
O	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
O	0051603	biological_process	proteolysis involved in protein catabolic process
O	0070062	cellular_component	extracellular exosome
P	0000502	cellular_component	proteasome complex
P	0000932	cellular_component	P-body
P	0005515	molecular_function	protein binding
P	0005576	cellular_component	extracellular region
P	0005634	cellular_component	nucleus
P	0005654	cellular_component	nucleoplasm
P	0005737	cellular_component	cytoplasm
P	0005829	cellular_component	cytosol
P	0005839	cellular_component	proteasome core complex
P	0006511	biological_process	ubiquitin-dependent protein catabolic process
P	0009615	biological_process	response to virus
P	0019773	cellular_component	proteasome core complex, alpha-subunit complex
P	0034774	cellular_component	secretory granule lumen
P	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
P	0051603	biological_process	proteolysis involved in protein catabolic process
P	0070062	cellular_component	extracellular exosome
P	1904813	cellular_component	ficolin-1-rich granule lumen
Q	0000502	cellular_component	proteasome complex
Q	0000932	cellular_component	P-body
Q	0005515	molecular_function	protein binding
Q	0005634	cellular_component	nucleus
Q	0005654	cellular_component	nucleoplasm
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0005839	cellular_component	proteasome core complex
Q	0006511	biological_process	ubiquitin-dependent protein catabolic process
Q	0019773	cellular_component	proteasome core complex, alpha-subunit complex
Q	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
Q	0043231	cellular_component	intracellular membrane-bounded organelle
Q	0051603	biological_process	proteolysis involved in protein catabolic process
Q	0070062	cellular_component	extracellular exosome
R	0000502	cellular_component	proteasome complex
R	0005515	molecular_function	protein binding
R	0005634	cellular_component	nucleus
R	0005654	cellular_component	nucleoplasm
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0005839	cellular_component	proteasome core complex
R	0006511	biological_process	ubiquitin-dependent protein catabolic process
R	0019773	cellular_component	proteasome core complex, alpha-subunit complex
R	0042802	molecular_function	identical protein binding
R	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
R	0051603	biological_process	proteolysis involved in protein catabolic process
R	0070062	cellular_component	extracellular exosome
R	0098794	cellular_component	postsynapse
S	0000502	cellular_component	proteasome complex
S	0005515	molecular_function	protein binding
S	0005576	cellular_component	extracellular region
S	0005634	cellular_component	nucleus
S	0005654	cellular_component	nucleoplasm
S	0005737	cellular_component	cytoplasm
S	0005829	cellular_component	cytosol
S	0005839	cellular_component	proteasome core complex
S	0006511	biological_process	ubiquitin-dependent protein catabolic process
S	0019773	cellular_component	proteasome core complex, alpha-subunit complex
S	0034774	cellular_component	secretory granule lumen
S	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
S	0051603	biological_process	proteolysis involved in protein catabolic process
S	0070062	cellular_component	extracellular exosome
S	1904813	cellular_component	ficolin-1-rich granule lumen
T	0000502	cellular_component	proteasome complex
T	0001530	molecular_function	lipopolysaccharide binding
T	0002376	biological_process	immune system process
T	0002862	biological_process	negative regulation of inflammatory response to antigenic stimulus
T	0005515	molecular_function	protein binding
T	0005634	cellular_component	nucleus
T	0005654	cellular_component	nucleoplasm
T	0005737	cellular_component	cytoplasm
T	0005813	cellular_component	centrosome
T	0005829	cellular_component	cytosol
T	0005839	cellular_component	proteasome core complex
T	0006511	biological_process	ubiquitin-dependent protein catabolic process
T	0019773	cellular_component	proteasome core complex, alpha-subunit complex
T	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
T	0051603	biological_process	proteolysis involved in protein catabolic process
T	0070062	cellular_component	extracellular exosome
U	0000502	cellular_component	proteasome complex
U	0005515	molecular_function	protein binding
U	0005634	cellular_component	nucleus
U	0005654	cellular_component	nucleoplasm
U	0005737	cellular_component	cytoplasm
U	0005829	cellular_component	cytosol
U	0005839	cellular_component	proteasome core complex
U	0006511	biological_process	ubiquitin-dependent protein catabolic process
U	0019773	cellular_component	proteasome core complex, alpha-subunit complex
U	0031625	molecular_function	ubiquitin protein ligase binding
U	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
U	0045202	cellular_component	synapse
U	0051603	biological_process	proteolysis involved in protein catabolic process
U	0052548	biological_process	regulation of endopeptidase activity
U	0070062	cellular_component	extracellular exosome
V	0000502	cellular_component	proteasome complex
V	0000932	cellular_component	P-body
V	0003723	molecular_function	RNA binding
V	0004175	molecular_function	endopeptidase activity
V	0005515	molecular_function	protein binding
V	0005634	cellular_component	nucleus
V	0005654	cellular_component	nucleoplasm
V	0005737	cellular_component	cytoplasm
V	0005829	cellular_component	cytosol
V	0005839	cellular_component	proteasome core complex
V	0005840	cellular_component	ribosome
V	0006511	biological_process	ubiquitin-dependent protein catabolic process
V	0016363	cellular_component	nuclear matrix
V	0019773	cellular_component	proteasome core complex, alpha-subunit complex
V	0030016	cellular_component	myofibril
V	0030017	cellular_component	sarcomere
V	0035639	molecular_function	purine ribonucleoside triphosphate binding
V	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
V	0050727	biological_process	regulation of inflammatory response
V	0051059	molecular_function	NF-kappaB binding
V	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
V	0051603	biological_process	proteolysis involved in protein catabolic process
V	0070062	cellular_component	extracellular exosome
W	0000502	cellular_component	proteasome complex
W	0000932	cellular_component	P-body
W	0005515	molecular_function	protein binding
W	0005576	cellular_component	extracellular region
W	0005634	cellular_component	nucleus
W	0005654	cellular_component	nucleoplasm
W	0005737	cellular_component	cytoplasm
W	0005829	cellular_component	cytosol
W	0005839	cellular_component	proteasome core complex
W	0006511	biological_process	ubiquitin-dependent protein catabolic process
W	0009615	biological_process	response to virus
W	0019773	cellular_component	proteasome core complex, alpha-subunit complex
W	0034774	cellular_component	secretory granule lumen
W	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
W	0051603	biological_process	proteolysis involved in protein catabolic process
W	0070062	cellular_component	extracellular exosome
W	1904813	cellular_component	ficolin-1-rich granule lumen
X	0000502	cellular_component	proteasome complex
X	0000932	cellular_component	P-body
X	0005515	molecular_function	protein binding
X	0005634	cellular_component	nucleus
X	0005654	cellular_component	nucleoplasm
X	0005737	cellular_component	cytoplasm
X	0005829	cellular_component	cytosol
X	0005839	cellular_component	proteasome core complex
X	0006511	biological_process	ubiquitin-dependent protein catabolic process
X	0019773	cellular_component	proteasome core complex, alpha-subunit complex
X	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
X	0043231	cellular_component	intracellular membrane-bounded organelle
X	0051603	biological_process	proteolysis involved in protein catabolic process
X	0070062	cellular_component	extracellular exosome
Y	0000502	cellular_component	proteasome complex
Y	0005515	molecular_function	protein binding
Y	0005634	cellular_component	nucleus
Y	0005654	cellular_component	nucleoplasm
Y	0005737	cellular_component	cytoplasm
Y	0005829	cellular_component	cytosol
Y	0005839	cellular_component	proteasome core complex
Y	0006511	biological_process	ubiquitin-dependent protein catabolic process
Y	0019773	cellular_component	proteasome core complex, alpha-subunit complex
Y	0042802	molecular_function	identical protein binding
Y	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
Y	0051603	biological_process	proteolysis involved in protein catabolic process
Y	0070062	cellular_component	extracellular exosome
Y	0098794	cellular_component	postsynapse
Z	0000502	cellular_component	proteasome complex
Z	0005515	molecular_function	protein binding
Z	0005576	cellular_component	extracellular region
Z	0005634	cellular_component	nucleus
Z	0005654	cellular_component	nucleoplasm
Z	0005737	cellular_component	cytoplasm
Z	0005829	cellular_component	cytosol
Z	0005839	cellular_component	proteasome core complex
Z	0006511	biological_process	ubiquitin-dependent protein catabolic process
Z	0019773	cellular_component	proteasome core complex, alpha-subunit complex
Z	0034774	cellular_component	secretory granule lumen
Z	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
Z	0051603	biological_process	proteolysis involved in protein catabolic process
Z	0070062	cellular_component	extracellular exosome
Z	1904813	cellular_component	ficolin-1-rich granule lumen

Functional Information from PROSITE/UniProt

site_id	PS00388
Number of Residues	23
Details	PROTEASOME_ALPHA_1 Proteasome alpha-type subunits signature. YdndvTvWSPqGRihQIEYAmeA

Chain	Residue	Details
T	TYR6-ALA28
X	TYR5-ALA27
V	PHE10-ALA32
W	TYR5-ALA27
Z	TYR8-ALA30
Y	TYR3-ALA25
U	TYR7-ALA29

---

site_id	PS00854
Number of Residues	48
Details	PROTEASOME_BETA_1 Proteasome beta-type subunits signature. LGVkFegGVVIAADmlgsygslarfrnis.RimrvnnstmlgasGdyAD

Chain	Residue	Details
G	LEU12-ASP59
B	ALA4-ASP51
X	LEU35-ASP82
F	LEU13-ASP60
A	MET4-ASP51
E	LEU4-ASP51
C	MET11-ASP58
D	ILE5-ASP52

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	MOD_RES: N-acetylalanine => ECO:0007744|PubMed:19413330

Chain	Residue	Details
W	ALA1
P	ALA1
V	SER65
O	SER17
O	SER63
O	SER64

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P49722

Chain	Residue	Details
W	TYR5
P	TYR5
O	LYS102
O	LYS104
U	LYS205
U	LYS229

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
W	SER6
W	SER13
W	SER15
P	SER6
P	SER13
P	SER15

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:15592455

Chain	Residue	Details
W	TYR23
P	TYR23
M	SER30
M	SER181

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
W	LYS69
W	LYS170
P	LYS69
P	LYS170
T	LYS208

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
W	TYR75
P	TYR75

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P17220

Chain	Residue	Details
W	TYR120
P	TYR120

---