7TW3
Cryo-EM structure of human ankyrin complex (B2P1A1) from red blood cell
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005452 | molecular_function | solute:inorganic anion antiporter activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006811 | biological_process | monoatomic ion transport |
| A | 0006820 | biological_process | monoatomic anion transport |
| A | 0006821 | biological_process | chloride transport |
| A | 0006873 | biological_process | intracellular monoatomic ion homeostasis |
| A | 0007596 | biological_process | blood coagulation |
| A | 0008092 | molecular_function | cytoskeletal protein binding |
| A | 0008509 | molecular_function | monoatomic anion transmembrane transporter activity |
| A | 0009898 | cellular_component | cytoplasmic side of plasma membrane |
| A | 0015106 | molecular_function | bicarbonate transmembrane transporter activity |
| A | 0015108 | molecular_function | chloride transmembrane transporter activity |
| A | 0015297 | molecular_function | antiporter activity |
| A | 0015701 | biological_process | bicarbonate transport |
| A | 0016020 | cellular_component | membrane |
| A | 0016323 | cellular_component | basolateral plasma membrane |
| A | 0017121 | biological_process | plasma membrane phospholipid scrambling |
| A | 0030018 | cellular_component | Z disc |
| A | 0030492 | molecular_function | hemoglobin binding |
| A | 0030506 | molecular_function | ankyrin binding |
| A | 0030863 | cellular_component | cortical cytoskeleton |
| A | 0035811 | biological_process | negative regulation of urine volume |
| A | 0036296 | biological_process | response to increased oxygen levels |
| A | 0042803 | molecular_function | protein homodimerization activity |
| A | 0043495 | molecular_function | protein-membrane adaptor activity |
| A | 0045852 | biological_process | pH elevation |
| A | 0048821 | biological_process | erythrocyte development |
| A | 0051453 | biological_process | regulation of intracellular pH |
| A | 0055085 | biological_process | transmembrane transport |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 0072562 | cellular_component | blood microparticle |
| A | 0072659 | biological_process | protein localization to plasma membrane |
| A | 0140900 | molecular_function | chloride:bicarbonate antiporter activity |
| A | 0170014 | cellular_component | ankyrin-1 complex |
| A | 1902476 | biological_process | chloride transmembrane transport |
| A | 1904539 | biological_process | negative regulation of glycolytic process through fructose-6-phosphate |
| B | 0005452 | molecular_function | solute:inorganic anion antiporter activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0006811 | biological_process | monoatomic ion transport |
| B | 0006820 | biological_process | monoatomic anion transport |
| B | 0006821 | biological_process | chloride transport |
| B | 0006873 | biological_process | intracellular monoatomic ion homeostasis |
| B | 0007596 | biological_process | blood coagulation |
| B | 0008092 | molecular_function | cytoskeletal protein binding |
| B | 0008509 | molecular_function | monoatomic anion transmembrane transporter activity |
| B | 0009898 | cellular_component | cytoplasmic side of plasma membrane |
| B | 0015106 | molecular_function | bicarbonate transmembrane transporter activity |
| B | 0015108 | molecular_function | chloride transmembrane transporter activity |
| B | 0015297 | molecular_function | antiporter activity |
| B | 0015701 | biological_process | bicarbonate transport |
| B | 0016020 | cellular_component | membrane |
| B | 0016323 | cellular_component | basolateral plasma membrane |
| B | 0017121 | biological_process | plasma membrane phospholipid scrambling |
| B | 0030018 | cellular_component | Z disc |
| B | 0030492 | molecular_function | hemoglobin binding |
| B | 0030506 | molecular_function | ankyrin binding |
| B | 0030863 | cellular_component | cortical cytoskeleton |
| B | 0035811 | biological_process | negative regulation of urine volume |
| B | 0036296 | biological_process | response to increased oxygen levels |
| B | 0042803 | molecular_function | protein homodimerization activity |
| B | 0043495 | molecular_function | protein-membrane adaptor activity |
| B | 0045852 | biological_process | pH elevation |
| B | 0048821 | biological_process | erythrocyte development |
| B | 0051453 | biological_process | regulation of intracellular pH |
| B | 0055085 | biological_process | transmembrane transport |
| B | 0070062 | cellular_component | extracellular exosome |
| B | 0072562 | cellular_component | blood microparticle |
| B | 0072659 | biological_process | protein localization to plasma membrane |
| B | 0140900 | molecular_function | chloride:bicarbonate antiporter activity |
| B | 0170014 | cellular_component | ankyrin-1 complex |
| B | 1902476 | biological_process | chloride transmembrane transport |
| B | 1904539 | biological_process | negative regulation of glycolytic process through fructose-6-phosphate |
| E | 0000902 | biological_process | cell morphogenesis |
| E | 0003810 | molecular_function | protein-glutamine gamma-glutamyltransferase activity |
| E | 0005200 | molecular_function | structural constituent of cytoskeleton |
| E | 0005515 | molecular_function | protein binding |
| E | 0005524 | molecular_function | ATP binding |
| E | 0005856 | cellular_component | cytoskeleton |
| E | 0005886 | cellular_component | plasma membrane |
| E | 0007010 | biological_process | cytoskeleton organization |
| E | 0008360 | biological_process | regulation of cell shape |
| E | 0016020 | cellular_component | membrane |
| E | 0018149 | biological_process | peptide cross-linking |
| E | 0020027 | biological_process | hemoglobin metabolic process |
| E | 0030863 | cellular_component | cortical cytoskeleton |
| E | 0043249 | biological_process | erythrocyte maturation |
| E | 0048536 | biological_process | spleen development |
| E | 0050801 | biological_process | monoatomic ion homeostasis |
| E | 0060586 | biological_process | multicellular organismal-level iron ion homeostasis |
| E | 0071944 | cellular_component | cell periphery |
| E | 0170014 | cellular_component | ankyrin-1 complex |
| G | 0005198 | molecular_function | structural molecule activity |
| G | 0005200 | molecular_function | structural constituent of cytoskeleton |
| G | 0005515 | molecular_function | protein binding |
| G | 0005737 | cellular_component | cytoplasm |
| G | 0005829 | cellular_component | cytosol |
| G | 0005856 | cellular_component | cytoskeleton |
| G | 0005886 | cellular_component | plasma membrane |
| G | 0006887 | biological_process | exocytosis |
| G | 0006888 | biological_process | endoplasmic reticulum to Golgi vesicle-mediated transport |
| G | 0007010 | biological_process | cytoskeleton organization |
| G | 0007165 | biological_process | signal transduction |
| G | 0008093 | molecular_function | cytoskeletal anchor activity |
| G | 0009898 | cellular_component | cytoplasmic side of plasma membrane |
| G | 0014731 | cellular_component | spectrin-associated cytoskeleton |
| G | 0016020 | cellular_component | membrane |
| G | 0016323 | cellular_component | basolateral plasma membrane |
| G | 0016529 | cellular_component | sarcoplasmic reticulum |
| G | 0019899 | molecular_function | enzyme binding |
| G | 0019903 | molecular_function | protein phosphatase binding |
| G | 0030018 | cellular_component | Z disc |
| G | 0030507 | molecular_function | spectrin binding |
| G | 0030673 | cellular_component | axolemma |
| G | 0031430 | cellular_component | M band |
| G | 0031672 | cellular_component | A band |
| G | 0042383 | cellular_component | sarcolemma |
| G | 0043005 | cellular_component | neuron projection |
| G | 0044325 | molecular_function | transmembrane transporter binding |
| G | 0045199 | biological_process | maintenance of epithelial cell apical/basal polarity |
| G | 0045211 | cellular_component | postsynaptic membrane |
| G | 0048193 | biological_process | Golgi vesicle transport |
| G | 0051117 | molecular_function | ATPase binding |
| G | 0071944 | cellular_component | cell periphery |
| G | 0072659 | biological_process | protein localization to plasma membrane |
| G | 0170014 | cellular_component | ankyrin-1 complex |
Functional Information from PROSITE/UniProt
| site_id | PS00219 |
| Number of Residues | 12 |
| Details | ANION_EXCHANGER_1 Anion exchangers family signature 1. FGGLVRDIRRRY |
| Chain | Residue | Details |
| A | PHE379-TYR390 |
| site_id | PS00220 |
| Number of Residues | 15 |
| Details | ANION_EXCHANGER_2 Anion exchangers family signature 2. FLISLIFIYETFsKL |
| Chain | Residue | Details |
| A | PHE526-LEU540 |
| site_id | PS00547 |
| Number of Residues | 18 |
| Details | TRANSGLUTAMINASES Transglutaminases active site. GQAWVlAAVacTvLRCLG |
| Chain | Residue | Details |
| E | GLY266-GLY283 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 46 |
| Details | Transmembrane: {"description":"Helical; Name=1","evidences":[{"source":"PubMed","id":"26542571","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 164 |
| Details | Topological domain: {"description":"Extracellular","evidences":[{"source":"PubMed","id":"26542571","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 40 |
| Details | Transmembrane: {"description":"Helical; Name=2","evidences":[{"source":"PubMed","id":"26542571","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 150 |
| Details | Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"26542571","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 32 |
| Details | Transmembrane: {"description":"Discontinuously helical; Name=3","evidences":[{"source":"PubMed","id":"26542571","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 40 |
| Details | Transmembrane: {"description":"Helical; Name=4","evidences":[{"source":"PubMed","id":"26542571","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 44 |
| Details | Transmembrane: {"description":"Helical; Name=5","evidences":[{"source":"PubMed","id":"26542571","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 40 |
| Details | Transmembrane: {"description":"Helical; Name=6","evidences":[{"source":"PubMed","id":"26542571","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 40 |
| Details | Transmembrane: {"description":"Helical; Name=7","evidences":[{"source":"PubMed","id":"26542571","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 40 |
| Details | Transmembrane: {"description":"Helical; Name=8","evidences":[{"source":"PubMed","id":"26542571","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 36 |
| Details | Transmembrane: {"description":"Helical; Name=9","evidences":[{"source":"PubMed","id":"26542571","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 34 |
| Details | Transmembrane: {"description":"Discontinuously helical; Name=10","evidences":[{"source":"PubMed","id":"26542571","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI13 |
| Number of Residues | 40 |
| Details | Transmembrane: {"description":"Helical; Name=11","evidences":[{"source":"PubMed","id":"26542571","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI14 |
| Number of Residues | 36 |
| Details | Transmembrane: {"description":"Helical; Name=12","evidences":[{"source":"PubMed","id":"26542571","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI15 |
| Number of Residues | 60 |
| Details | Intramembrane: {"description":"Discontinuously helical","evidences":[{"source":"PubMed","id":"26542571","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI16 |
| Number of Residues | 18 |
| Details | Region: {"description":"Interaction with ANK1","evidences":[{"evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI17 |
| Number of Residues | 142 |
| Details | Region: {"description":"Involved in anion transport"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI18 |
| Number of Residues | 2 |
| Details | Site: {"description":"Important for anion transport"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI19 |
| Number of Residues | 2 |
| Details | Site: {"description":"Important for anion-proton cotransport"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI20 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"PubMed","id":"1998697","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI21 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P23562","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI22 |
| Number of Residues | 2 |
| Details | Lipidation: {"description":"S-palmitoyl cysteine","evidences":[{"source":"PubMed","id":"1885574","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI23 |
| Number of Residues | 2 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) (complex) asparagine","evidences":[{"source":"PubMed","id":"10861210","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24121512","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"35835865","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI24 |
| Number of Residues | 53 |
| Details | Region: {"description":"Dimerization arm"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI25 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P04919","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI26 |
| Number of Residues | 8 |
| Details | Region: {"description":"Band 3 binding","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI27 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine; by PKA","evidences":[{"source":"PubMed","id":"8499466","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI28 |
| Number of Residues | 27 |
| Details | Repeat: {"description":"ANK 5"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI29 |
| Number of Residues | 29 |
| Details | Repeat: {"description":"ANK 6"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI30 |
| Number of Residues | 29 |
| Details | Repeat: {"description":"ANK 7"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI31 |
| Number of Residues | 29 |
| Details | Repeat: {"description":"ANK 8"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI32 |
| Number of Residues | 29 |
| Details | Repeat: {"description":"ANK 9"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI33 |
| Number of Residues | 29 |
| Details | Repeat: {"description":"ANK 10"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI34 |
| Number of Residues | 29 |
| Details | Repeat: {"description":"ANK 11"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI35 |
| Number of Residues | 29 |
| Details | Repeat: {"description":"ANK 12"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI36 |
| Number of Residues | 29 |
| Details | Repeat: {"description":"ANK 13"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI37 |
| Number of Residues | 29 |
| Details | Repeat: {"description":"ANK 14"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI38 |
| Number of Residues | 29 |
| Details | Repeat: {"description":"ANK 15"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI39 |
| Number of Residues | 29 |
| Details | Repeat: {"description":"ANK 16"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI40 |
| Number of Residues | 29 |
| Details | Repeat: {"description":"ANK 17"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI41 |
| Number of Residues | 29 |
| Details | Repeat: {"description":"ANK 18"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI42 |
| Number of Residues | 29 |
| Details | Repeat: {"description":"ANK 19"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI43 |
| Number of Residues | 29 |
| Details | Repeat: {"description":"ANK 20"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI44 |
| Number of Residues | 29 |
| Details | Repeat: {"description":"ANK 21"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI45 |
| Number of Residues | 29 |
| Details | Repeat: {"description":"ANK 22"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI46 |
| Number of Residues | 29 |
| Details | Repeat: {"description":"ANK 23"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI47 |
| Number of Residues | 7 |
| Details | Modified residue: {"description":"(3S)-3-hydroxyasparagine; by HIF1AN; partial","evidences":[{"source":"PubMed","id":"21177872","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI48 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI49 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"(3S)-3-hydroxyaspartate; by HIF1AN; partial","evidences":[{"source":"PubMed","id":"21177872","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI50 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
