Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7TSW

Crystal Structure Of Human NADH-Cytochrome B5 Reductase T117D Mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004128	molecular_function	cytochrome-b5 reductase activity, acting on NAD(P)H
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005741	cellular_component	mitochondrial outer membrane
A	0005783	cellular_component	endoplasmic reticulum
A	0005789	cellular_component	endoplasmic reticulum membrane
A	0005811	cellular_component	lipid droplet
A	0005833	cellular_component	hemoglobin complex
A	0006629	biological_process	lipid metabolic process
A	0006694	biological_process	steroid biosynthetic process
A	0006695	biological_process	cholesterol biosynthetic process
A	0006809	biological_process	nitric oxide biosynthetic process
A	0008015	biological_process	blood circulation
A	0008202	biological_process	steroid metabolic process
A	0008203	biological_process	cholesterol metabolic process
A	0016020	cellular_component	membrane
A	0016126	biological_process	sterol biosynthetic process
A	0016491	molecular_function	oxidoreductase activity
A	0031966	cellular_component	mitochondrial membrane
A	0035578	cellular_component	azurophil granule lumen
A	0050421	molecular_function	nitrite reductase (NO-forming) activity
A	0071949	molecular_function	FAD binding
A	1903958	cellular_component	nitric-oxide synthase complex
B	0004128	molecular_function	cytochrome-b5 reductase activity, acting on NAD(P)H
B	0005515	molecular_function	protein binding
B	0005576	cellular_component	extracellular region
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005741	cellular_component	mitochondrial outer membrane
B	0005783	cellular_component	endoplasmic reticulum
B	0005789	cellular_component	endoplasmic reticulum membrane
B	0005811	cellular_component	lipid droplet
B	0005833	cellular_component	hemoglobin complex
B	0006629	biological_process	lipid metabolic process
B	0006694	biological_process	steroid biosynthetic process
B	0006695	biological_process	cholesterol biosynthetic process
B	0006809	biological_process	nitric oxide biosynthetic process
B	0008015	biological_process	blood circulation
B	0008202	biological_process	steroid metabolic process
B	0008203	biological_process	cholesterol metabolic process
B	0016020	cellular_component	membrane
B	0016126	biological_process	sterol biosynthetic process
B	0016491	molecular_function	oxidoreductase activity
B	0031966	cellular_component	mitochondrial membrane
B	0035578	cellular_component	azurophil granule lumen
B	0050421	molecular_function	nitrite reductase (NO-forming) activity
B	0071949	molecular_function	FAD binding
B	1903958	cellular_component	nitric-oxide synthase complex
C	0004128	molecular_function	cytochrome-b5 reductase activity, acting on NAD(P)H
C	0005515	molecular_function	protein binding
C	0005576	cellular_component	extracellular region
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005741	cellular_component	mitochondrial outer membrane
C	0005783	cellular_component	endoplasmic reticulum
C	0005789	cellular_component	endoplasmic reticulum membrane
C	0005811	cellular_component	lipid droplet
C	0005833	cellular_component	hemoglobin complex
C	0006629	biological_process	lipid metabolic process
C	0006694	biological_process	steroid biosynthetic process
C	0006695	biological_process	cholesterol biosynthetic process
C	0006809	biological_process	nitric oxide biosynthetic process
C	0008015	biological_process	blood circulation
C	0008202	biological_process	steroid metabolic process
C	0008203	biological_process	cholesterol metabolic process
C	0016020	cellular_component	membrane
C	0016126	biological_process	sterol biosynthetic process
C	0016491	molecular_function	oxidoreductase activity
C	0031966	cellular_component	mitochondrial membrane
C	0035578	cellular_component	azurophil granule lumen
C	0050421	molecular_function	nitrite reductase (NO-forming) activity
C	0071949	molecular_function	FAD binding
C	1903958	cellular_component	nitric-oxide synthase complex
D	0004128	molecular_function	cytochrome-b5 reductase activity, acting on NAD(P)H
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005741	cellular_component	mitochondrial outer membrane
D	0005783	cellular_component	endoplasmic reticulum
D	0005789	cellular_component	endoplasmic reticulum membrane
D	0005811	cellular_component	lipid droplet
D	0005833	cellular_component	hemoglobin complex
D	0006629	biological_process	lipid metabolic process
D	0006694	biological_process	steroid biosynthetic process
D	0006695	biological_process	cholesterol biosynthetic process
D	0006809	biological_process	nitric oxide biosynthetic process
D	0008015	biological_process	blood circulation
D	0008202	biological_process	steroid metabolic process
D	0008203	biological_process	cholesterol metabolic process
D	0016020	cellular_component	membrane
D	0016126	biological_process	sterol biosynthetic process
D	0016491	molecular_function	oxidoreductase activity
D	0031966	cellular_component	mitochondrial membrane
D	0035578	cellular_component	azurophil granule lumen
D	0050421	molecular_function	nitrite reductase (NO-forming) activity
D	0071949	molecular_function	FAD binding
D	1903958	cellular_component	nitric-oxide synthase complex
E	0004128	molecular_function	cytochrome-b5 reductase activity, acting on NAD(P)H
E	0005515	molecular_function	protein binding
E	0005576	cellular_component	extracellular region
E	0005737	cellular_component	cytoplasm
E	0005739	cellular_component	mitochondrion
E	0005741	cellular_component	mitochondrial outer membrane
E	0005783	cellular_component	endoplasmic reticulum
E	0005789	cellular_component	endoplasmic reticulum membrane
E	0005811	cellular_component	lipid droplet
E	0005833	cellular_component	hemoglobin complex
E	0006629	biological_process	lipid metabolic process
E	0006694	biological_process	steroid biosynthetic process
E	0006695	biological_process	cholesterol biosynthetic process
E	0006809	biological_process	nitric oxide biosynthetic process
E	0008015	biological_process	blood circulation
E	0008202	biological_process	steroid metabolic process
E	0008203	biological_process	cholesterol metabolic process
E	0016020	cellular_component	membrane
E	0016126	biological_process	sterol biosynthetic process
E	0016491	molecular_function	oxidoreductase activity
E	0031966	cellular_component	mitochondrial membrane
E	0035578	cellular_component	azurophil granule lumen
E	0050421	molecular_function	nitrite reductase (NO-forming) activity
E	0071949	molecular_function	FAD binding
E	1903958	cellular_component	nitric-oxide synthase complex
F	0004128	molecular_function	cytochrome-b5 reductase activity, acting on NAD(P)H
F	0005515	molecular_function	protein binding
F	0005576	cellular_component	extracellular region
F	0005737	cellular_component	cytoplasm
F	0005739	cellular_component	mitochondrion
F	0005741	cellular_component	mitochondrial outer membrane
F	0005783	cellular_component	endoplasmic reticulum
F	0005789	cellular_component	endoplasmic reticulum membrane
F	0005811	cellular_component	lipid droplet
F	0005833	cellular_component	hemoglobin complex
F	0006629	biological_process	lipid metabolic process
F	0006694	biological_process	steroid biosynthetic process
F	0006695	biological_process	cholesterol biosynthetic process
F	0006809	biological_process	nitric oxide biosynthetic process
F	0008015	biological_process	blood circulation
F	0008202	biological_process	steroid metabolic process
F	0008203	biological_process	cholesterol metabolic process
F	0016020	cellular_component	membrane
F	0016126	biological_process	sterol biosynthetic process
F	0016491	molecular_function	oxidoreductase activity
F	0031966	cellular_component	mitochondrial membrane
F	0035578	cellular_component	azurophil granule lumen
F	0050421	molecular_function	nitrite reductase (NO-forming) activity
F	0071949	molecular_function	FAD binding
F	1903958	cellular_component	nitric-oxide synthase complex

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	60
Details	BINDING: BINDING => ECO:0000269\|PubMed:15502298, ECO:0007744\|PDB:1UMK

Chain	Residue	Details
A	ARG92
A	THR185
B	ARG92
B	PRO93
B	TYR94
B	VAL109
B	LYS111
B	PHE114
B	LYS126
B	MET127
B	SER128
A	PRO93
B	THR185
C	ARG92
C	PRO93
C	TYR94
C	VAL109
C	LYS111
C	PHE114
C	LYS126
C	MET127
C	SER128
A	TYR94
C	THR185
D	ARG92
D	PRO93
D	TYR94
D	VAL109
D	LYS111
D	PHE114
D	LYS126
D	MET127
D	SER128
A	VAL109
D	THR185
E	ARG92
E	PRO93
E	TYR94
E	VAL109
E	LYS111
E	PHE114
E	LYS126
E	MET127
E	SER128
A	LYS111
E	THR185
F	ARG92
F	PRO93
F	TYR94
F	VAL109
F	LYS111
F	PHE114
F	LYS126
F	MET127
F	SER128
A	PHE114
F	THR185
A	LYS126
A	MET127
A	SER128

site_id	SWS_FT_FI2
Number of Residues	6
Details	MOD_RES: N6-acetyllysine => ECO:0007744\|PubMed:19608861

Chain	Residue	Details
A	LYS42
B	LYS42
C	LYS42
D	LYS42
E	LYS42
F	LYS42

site_id	SWS_FT_FI3
Number of Residues	6
Details	MOD_RES: Phosphotyrosine => ECO:0007744\|PubMed:18669648

Chain	Residue	Details
A	TYR43
B	TYR43
C	TYR43
D	TYR43
E	TYR43
F	TYR43

site_id	SWS_FT_FI4
Number of Residues	6
Details	MOD_RES: N6-acetyllysine => ECO:0000250\|UniProtKB:Q9DCN2

Chain	Residue	Details
A	LYS120
B	LYS120
C	LYS120
D	LYS120
E	LYS120
F	LYS120

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)