7T5F

Botulinum neurotoxin Type B Light Chain complexed with nanobodies JLJ-G3 and JNE-B10

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004222	molecular_function	metalloendopeptidase activity
A	0006508	biological_process	proteolysis
A	0008270	molecular_function	zinc ion binding
D	0004222	molecular_function	metalloendopeptidase activity
D	0006508	biological_process	proteolysis
D	0008270	molecular_function	zinc ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue ZN A 501

Chain	Residue
A	HIS230
A	HIS234
A	GLU268
A	HOH647

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site_id	AC2
Number of Residues	1
Details	binding site for residue IOD A 503

Chain	Residue
A	ARG42

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site_id	AC3
Number of Residues	4
Details	binding site for residue ZN D 501

Chain	Residue
D	HIS230
D	HIS234
D	GLU268
D	HOH637

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site_id	AC4
Number of Residues	1
Details	binding site for residue IOD C 201

Chain	Residue
C	ARG71

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site_id	AC5
Number of Residues	1
Details	binding site for residue IOD B 201

Chain	Residue
B	GLY54

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site_id	AC6
Number of Residues	3
Details	binding site for residue IOD E 201

Chain	Residue
E	ASN53
E	GLY54
E	ILE56

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Functional Information from PROSITE/UniProt

site_id	PS00142
Number of Residues	10
Details	ZINC_PROTEASE Neutral zinc metallopeptidases, zinc-binding region signature. ILMHELIHVL

Chain	Residue	Details
A	ILE227-LEU236

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: ACT_SITE => ECO:0000255|PROSITE-ProRule:PRU10095

Chain	Residue	Details
A	GLU231
D	GLU231

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU10095, ECO:0000269|PubMed:10932256, ECO:0000305|PubMed:1429690, ECO:0007744|PDB:1EPW, ECO:0007744|PDB:1F31, ECO:0007744|PDB:2NP0

Chain	Residue	Details
A	HIS230
A	HIS234
D	HIS230
D	HIS234

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:10932256, ECO:0007744|PDB:1EPW, ECO:0007744|PDB:1F31, ECO:0007744|PDB:2NP0

Chain	Residue	Details
A	GLU268
D	GLU268

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 626

Chain	Residue	Details
A	HIS230	metal ligand
A	GLU231	proton acceptor, proton donor
A	HIS234	metal ligand
A	GLU268	metal ligand
A	ARG370	electrostatic stabiliser

---

site_id	MCSA2
Number of Residues	5
Details	M-CSA 626

Chain	Residue	Details
D	HIS230	metal ligand
D	GLU231	proton acceptor, proton donor
D	HIS234	metal ligand
D	GLU268	metal ligand
D	ARG370	electrostatic stabiliser

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