7SJ9

13pf E254A microtubule from recombinant human tubulin decorated with EB3

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000226	biological_process	microtubule cytoskeleton organization
A	0000278	biological_process	mitotic cell cycle
A	0003725	molecular_function	double-stranded RNA binding
A	0003924	molecular_function	GTPase activity
A	0005198	molecular_function	structural molecule activity
A	0005200	molecular_function	structural constituent of cytoskeleton
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005737	cellular_component	cytoplasm
A	0005856	cellular_component	cytoskeleton
A	0005874	cellular_component	microtubule
A	0005881	cellular_component	cytoplasmic microtubule
A	0005929	cellular_component	cilium
A	0007017	biological_process	microtubule-based process
A	0015630	cellular_component	microtubule cytoskeleton
A	0016787	molecular_function	hydrolase activity
A	0030705	biological_process	cytoskeleton-dependent intracellular transport
A	0031625	molecular_function	ubiquitin protein ligase binding
A	0051301	biological_process	cell division
A	0071353	biological_process	cellular response to interleukin-4
B	0000166	molecular_function	nucleotide binding
B	0000226	biological_process	microtubule cytoskeleton organization
B	0000278	biological_process	mitotic cell cycle
B	0003924	molecular_function	GTPase activity
B	0005200	molecular_function	structural constituent of cytoskeleton
B	0005515	molecular_function	protein binding
B	0005525	molecular_function	GTP binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005856	cellular_component	cytoskeleton
B	0005874	cellular_component	microtubule
B	0007017	biological_process	microtubule-based process
B	0007411	biological_process	axon guidance
B	0015630	cellular_component	microtubule cytoskeleton
B	0030027	cellular_component	lamellipodium
B	0030175	cellular_component	filopodium
B	0030182	biological_process	neuron differentiation
B	0030424	cellular_component	axon
B	0030425	cellular_component	dendrite
B	0030426	cellular_component	growth cone
B	0038007	biological_process	netrin-activated signaling pathway
B	0042277	molecular_function	peptide binding
B	0042995	cellular_component	cell projection
B	0043025	cellular_component	neuronal cell body
B	0045171	cellular_component	intercellular bridge
B	0046872	molecular_function	metal ion binding
B	0070062	cellular_component	extracellular exosome
B	0071944	cellular_component	cell periphery
B	0072686	cellular_component	mitotic spindle
B	1990791	biological_process	dorsal root ganglion development
B	1990890	molecular_function	netrin receptor binding
C	0000166	molecular_function	nucleotide binding
C	0000226	biological_process	microtubule cytoskeleton organization
C	0000278	biological_process	mitotic cell cycle
C	0003725	molecular_function	double-stranded RNA binding
C	0003924	molecular_function	GTPase activity
C	0005198	molecular_function	structural molecule activity
C	0005200	molecular_function	structural constituent of cytoskeleton
C	0005515	molecular_function	protein binding
C	0005525	molecular_function	GTP binding
C	0005737	cellular_component	cytoplasm
C	0005856	cellular_component	cytoskeleton
C	0005874	cellular_component	microtubule
C	0005881	cellular_component	cytoplasmic microtubule
C	0005929	cellular_component	cilium
C	0007017	biological_process	microtubule-based process
C	0015630	cellular_component	microtubule cytoskeleton
C	0016787	molecular_function	hydrolase activity
C	0030705	biological_process	cytoskeleton-dependent intracellular transport
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0051301	biological_process	cell division
C	0071353	biological_process	cellular response to interleukin-4
D	0000166	molecular_function	nucleotide binding
D	0000226	biological_process	microtubule cytoskeleton organization
D	0000278	biological_process	mitotic cell cycle
D	0003924	molecular_function	GTPase activity
D	0005200	molecular_function	structural constituent of cytoskeleton
D	0005515	molecular_function	protein binding
D	0005525	molecular_function	GTP binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005856	cellular_component	cytoskeleton
D	0005874	cellular_component	microtubule
D	0007017	biological_process	microtubule-based process
D	0007411	biological_process	axon guidance
D	0015630	cellular_component	microtubule cytoskeleton
D	0030027	cellular_component	lamellipodium
D	0030175	cellular_component	filopodium
D	0030182	biological_process	neuron differentiation
D	0030424	cellular_component	axon
D	0030425	cellular_component	dendrite
D	0030426	cellular_component	growth cone
D	0038007	biological_process	netrin-activated signaling pathway
D	0042277	molecular_function	peptide binding
D	0042995	cellular_component	cell projection
D	0043025	cellular_component	neuronal cell body
D	0045171	cellular_component	intercellular bridge
D	0046872	molecular_function	metal ion binding
D	0070062	cellular_component	extracellular exosome
D	0071944	cellular_component	cell periphery
D	0072686	cellular_component	mitotic spindle
D	1990791	biological_process	dorsal root ganglion development
D	1990890	molecular_function	netrin receptor binding
E	0000166	molecular_function	nucleotide binding
E	0000226	biological_process	microtubule cytoskeleton organization
E	0000278	biological_process	mitotic cell cycle
E	0003725	molecular_function	double-stranded RNA binding
E	0003924	molecular_function	GTPase activity
E	0005198	molecular_function	structural molecule activity
E	0005200	molecular_function	structural constituent of cytoskeleton
E	0005515	molecular_function	protein binding
E	0005525	molecular_function	GTP binding
E	0005737	cellular_component	cytoplasm
E	0005856	cellular_component	cytoskeleton
E	0005874	cellular_component	microtubule
E	0005881	cellular_component	cytoplasmic microtubule
E	0005929	cellular_component	cilium
E	0007017	biological_process	microtubule-based process
E	0015630	cellular_component	microtubule cytoskeleton
E	0016787	molecular_function	hydrolase activity
E	0030705	biological_process	cytoskeleton-dependent intracellular transport
E	0031625	molecular_function	ubiquitin protein ligase binding
E	0051301	biological_process	cell division
E	0071353	biological_process	cellular response to interleukin-4
F	0000166	molecular_function	nucleotide binding
F	0000226	biological_process	microtubule cytoskeleton organization
F	0000278	biological_process	mitotic cell cycle
F	0003924	molecular_function	GTPase activity
F	0005200	molecular_function	structural constituent of cytoskeleton
F	0005515	molecular_function	protein binding
F	0005525	molecular_function	GTP binding
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005856	cellular_component	cytoskeleton
F	0005874	cellular_component	microtubule
F	0007017	biological_process	microtubule-based process
F	0007411	biological_process	axon guidance
F	0015630	cellular_component	microtubule cytoskeleton
F	0030027	cellular_component	lamellipodium
F	0030175	cellular_component	filopodium
F	0030182	biological_process	neuron differentiation
F	0030424	cellular_component	axon
F	0030425	cellular_component	dendrite
F	0030426	cellular_component	growth cone
F	0038007	biological_process	netrin-activated signaling pathway
F	0042277	molecular_function	peptide binding
F	0042995	cellular_component	cell projection
F	0043025	cellular_component	neuronal cell body
F	0045171	cellular_component	intercellular bridge
F	0046872	molecular_function	metal ion binding
F	0070062	cellular_component	extracellular exosome
F	0071944	cellular_component	cell periphery
F	0072686	cellular_component	mitotic spindle
F	1990791	biological_process	dorsal root ganglion development
F	1990890	molecular_function	netrin receptor binding
G	0000166	molecular_function	nucleotide binding
G	0000226	biological_process	microtubule cytoskeleton organization
G	0000278	biological_process	mitotic cell cycle
G	0003924	molecular_function	GTPase activity
G	0005200	molecular_function	structural constituent of cytoskeleton
G	0005515	molecular_function	protein binding
G	0005525	molecular_function	GTP binding
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0005856	cellular_component	cytoskeleton
G	0005874	cellular_component	microtubule
G	0007017	biological_process	microtubule-based process
G	0007411	biological_process	axon guidance
G	0015630	cellular_component	microtubule cytoskeleton
G	0030027	cellular_component	lamellipodium
G	0030175	cellular_component	filopodium
G	0030182	biological_process	neuron differentiation
G	0030424	cellular_component	axon
G	0030425	cellular_component	dendrite
G	0030426	cellular_component	growth cone
G	0038007	biological_process	netrin-activated signaling pathway
G	0042277	molecular_function	peptide binding
G	0042995	cellular_component	cell projection
G	0043025	cellular_component	neuronal cell body
G	0045171	cellular_component	intercellular bridge
G	0046872	molecular_function	metal ion binding
G	0070062	cellular_component	extracellular exosome
G	0071944	cellular_component	cell periphery
G	0072686	cellular_component	mitotic spindle
G	1990791	biological_process	dorsal root ganglion development
G	1990890	molecular_function	netrin receptor binding
H	0000166	molecular_function	nucleotide binding
H	0000226	biological_process	microtubule cytoskeleton organization
H	0000278	biological_process	mitotic cell cycle
H	0003924	molecular_function	GTPase activity
H	0005200	molecular_function	structural constituent of cytoskeleton
H	0005515	molecular_function	protein binding
H	0005525	molecular_function	GTP binding
H	0005634	cellular_component	nucleus
H	0005737	cellular_component	cytoplasm
H	0005856	cellular_component	cytoskeleton
H	0005874	cellular_component	microtubule
H	0007017	biological_process	microtubule-based process
H	0007411	biological_process	axon guidance
H	0015630	cellular_component	microtubule cytoskeleton
H	0030027	cellular_component	lamellipodium
H	0030175	cellular_component	filopodium
H	0030182	biological_process	neuron differentiation
H	0030424	cellular_component	axon
H	0030425	cellular_component	dendrite
H	0030426	cellular_component	growth cone
H	0038007	biological_process	netrin-activated signaling pathway
H	0042277	molecular_function	peptide binding
H	0042995	cellular_component	cell projection
H	0043025	cellular_component	neuronal cell body
H	0045171	cellular_component	intercellular bridge
H	0046872	molecular_function	metal ion binding
H	0070062	cellular_component	extracellular exosome
H	0071944	cellular_component	cell periphery
H	0072686	cellular_component	mitotic spindle
H	1990791	biological_process	dorsal root ganglion development
H	1990890	molecular_function	netrin receptor binding
I	0000166	molecular_function	nucleotide binding
I	0000226	biological_process	microtubule cytoskeleton organization
I	0000278	biological_process	mitotic cell cycle
I	0003924	molecular_function	GTPase activity
I	0005200	molecular_function	structural constituent of cytoskeleton
I	0005515	molecular_function	protein binding
I	0005525	molecular_function	GTP binding
I	0005634	cellular_component	nucleus
I	0005737	cellular_component	cytoplasm
I	0005856	cellular_component	cytoskeleton
I	0005874	cellular_component	microtubule
I	0007017	biological_process	microtubule-based process
I	0007411	biological_process	axon guidance
I	0015630	cellular_component	microtubule cytoskeleton
I	0030027	cellular_component	lamellipodium
I	0030175	cellular_component	filopodium
I	0030182	biological_process	neuron differentiation
I	0030424	cellular_component	axon
I	0030425	cellular_component	dendrite
I	0030426	cellular_component	growth cone
I	0038007	biological_process	netrin-activated signaling pathway
I	0042277	molecular_function	peptide binding
I	0042995	cellular_component	cell projection
I	0043025	cellular_component	neuronal cell body
I	0045171	cellular_component	intercellular bridge
I	0046872	molecular_function	metal ion binding
I	0070062	cellular_component	extracellular exosome
I	0071944	cellular_component	cell periphery
I	0072686	cellular_component	mitotic spindle
I	1990791	biological_process	dorsal root ganglion development
I	1990890	molecular_function	netrin receptor binding
J	0000166	molecular_function	nucleotide binding
J	0000226	biological_process	microtubule cytoskeleton organization
J	0000278	biological_process	mitotic cell cycle
J	0003725	molecular_function	double-stranded RNA binding
J	0003924	molecular_function	GTPase activity
J	0005198	molecular_function	structural molecule activity
J	0005200	molecular_function	structural constituent of cytoskeleton
J	0005515	molecular_function	protein binding
J	0005525	molecular_function	GTP binding
J	0005737	cellular_component	cytoplasm
J	0005856	cellular_component	cytoskeleton
J	0005874	cellular_component	microtubule
J	0005881	cellular_component	cytoplasmic microtubule
J	0005929	cellular_component	cilium
J	0007017	biological_process	microtubule-based process
J	0015630	cellular_component	microtubule cytoskeleton
J	0016787	molecular_function	hydrolase activity
J	0030705	biological_process	cytoskeleton-dependent intracellular transport
J	0031625	molecular_function	ubiquitin protein ligase binding
J	0051301	biological_process	cell division
J	0071353	biological_process	cellular response to interleukin-4
K	0000166	molecular_function	nucleotide binding
K	0000226	biological_process	microtubule cytoskeleton organization
K	0000278	biological_process	mitotic cell cycle
K	0003725	molecular_function	double-stranded RNA binding
K	0003924	molecular_function	GTPase activity
K	0005198	molecular_function	structural molecule activity
K	0005200	molecular_function	structural constituent of cytoskeleton
K	0005515	molecular_function	protein binding
K	0005525	molecular_function	GTP binding
K	0005737	cellular_component	cytoplasm
K	0005856	cellular_component	cytoskeleton
K	0005874	cellular_component	microtubule
K	0005881	cellular_component	cytoplasmic microtubule
K	0005929	cellular_component	cilium
K	0007017	biological_process	microtubule-based process
K	0015630	cellular_component	microtubule cytoskeleton
K	0016787	molecular_function	hydrolase activity
K	0030705	biological_process	cytoskeleton-dependent intracellular transport
K	0031625	molecular_function	ubiquitin protein ligase binding
K	0051301	biological_process	cell division
K	0071353	biological_process	cellular response to interleukin-4
L	0000166	molecular_function	nucleotide binding
L	0000226	biological_process	microtubule cytoskeleton organization
L	0000278	biological_process	mitotic cell cycle
L	0003725	molecular_function	double-stranded RNA binding
L	0003924	molecular_function	GTPase activity
L	0005198	molecular_function	structural molecule activity
L	0005200	molecular_function	structural constituent of cytoskeleton
L	0005515	molecular_function	protein binding
L	0005525	molecular_function	GTP binding
L	0005737	cellular_component	cytoplasm
L	0005856	cellular_component	cytoskeleton
L	0005874	cellular_component	microtubule
L	0005881	cellular_component	cytoplasmic microtubule
L	0005929	cellular_component	cilium
L	0007017	biological_process	microtubule-based process
L	0015630	cellular_component	microtubule cytoskeleton
L	0016787	molecular_function	hydrolase activity
L	0030705	biological_process	cytoskeleton-dependent intracellular transport
L	0031625	molecular_function	ubiquitin protein ligase binding
L	0051301	biological_process	cell division
L	0071353	biological_process	cellular response to interleukin-4
M	0005515	molecular_function	protein binding
M	0005737	cellular_component	cytoplasm
M	0005815	cellular_component	microtubule organizing center
M	0005856	cellular_component	cytoskeleton
M	0005874	cellular_component	microtubule
M	0008017	molecular_function	microtubule binding
M	0008104	biological_process	intracellular protein localization
M	0015630	cellular_component	microtubule cytoskeleton
M	0030496	cellular_component	midbody
M	0031110	biological_process	regulation of microtubule polymerization or depolymerization
M	0031113	biological_process	regulation of microtubule polymerization
M	0035371	cellular_component	microtubule plus-end
M	0035372	biological_process	protein localization to microtubule
M	0042802	molecular_function	identical protein binding
M	0043539	molecular_function	protein serine/threonine kinase activator activity
M	0045893	biological_process	positive regulation of DNA-templated transcription
M	0048148	biological_process	behavioral response to cocaine
M	0051010	molecular_function	microtubule plus-end binding
M	0051225	biological_process	spindle assembly
M	0051233	cellular_component	spindle midzone
M	0051301	biological_process	cell division
M	0061003	biological_process	positive regulation of dendritic spine morphogenesis
M	0098984	cellular_component	neuron to neuron synapse
M	0120283	molecular_function	protein serine/threonine kinase binding
M	1905721	cellular_component	mitotic spindle astral microtubule end
M	1990752	cellular_component	microtubule end
N	0005515	molecular_function	protein binding
N	0005737	cellular_component	cytoplasm
N	0005815	cellular_component	microtubule organizing center
N	0005856	cellular_component	cytoskeleton
N	0005874	cellular_component	microtubule
N	0008017	molecular_function	microtubule binding
N	0008104	biological_process	intracellular protein localization
N	0015630	cellular_component	microtubule cytoskeleton
N	0030496	cellular_component	midbody
N	0031110	biological_process	regulation of microtubule polymerization or depolymerization
N	0031113	biological_process	regulation of microtubule polymerization
N	0035371	cellular_component	microtubule plus-end
N	0035372	biological_process	protein localization to microtubule
N	0042802	molecular_function	identical protein binding
N	0043539	molecular_function	protein serine/threonine kinase activator activity
N	0045893	biological_process	positive regulation of DNA-templated transcription
N	0048148	biological_process	behavioral response to cocaine
N	0051010	molecular_function	microtubule plus-end binding
N	0051225	biological_process	spindle assembly
N	0051233	cellular_component	spindle midzone
N	0051301	biological_process	cell division
N	0061003	biological_process	positive regulation of dendritic spine morphogenesis
N	0098984	cellular_component	neuron to neuron synapse
N	0120283	molecular_function	protein serine/threonine kinase binding
N	1905721	cellular_component	mitotic spindle astral microtubule end
N	1990752	cellular_component	microtubule end

Functional Information from PROSITE/UniProt

site_id	PS00227
Number of Residues	7
Details	TUBULIN Tubulin subunits alpha, beta, and gamma signature. GGGTGSG

Chain	Residue	Details
B	GLY140-GLY146
A	GLY142-GLY148

---

site_id	PS00228
Number of Residues	4
Details	TUBULIN_B_AUTOREG Tubulin-beta mRNA autoregulation signal. MREI

Chain	Residue	Details
B	MET1-ILE4

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	18
Details	Motif: {"description":"MREC motif","evidences":[{"source":"PubMed","id":"31727855","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	Active site: {"evidences":[{"source":"PubMed","id":"34996871","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	90
Details	Binding site: {"evidences":[{"source":"PubMed","id":"38609661","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VT7","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	48
Details	Binding site: {"evidences":[{"source":"PubMed","id":"34996871","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"35482892","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38609661","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7SJ7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7Z6S","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VT7","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	12
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"MAR-2009","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Waridel P.","Quadroni M."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	Modified residue: {"description":"3'-nitrotyrosine","evidences":[{"source":"UniProtKB","id":"P68373","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	6
Details	Modified residue: {"description":"Omega-N-methylarginine","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"MAR-2009","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Waridel P.","Quadroni M."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	6
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P68373","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	18
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)"}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	18
Details	Motif: {"description":"MREI motif","evidences":[{"source":"UniProtKB","id":"P07437","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	36
Details	Binding site: {"evidences":[{"source":"PubMed","id":"34996871","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"35482892","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"7SJ7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7Z6S","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P68363","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"34996871","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"7SJ7","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	6
Details	Modified residue: {"description":"Phosphoserine; by CDK1","evidences":[{"source":"PubMed","id":"16371510","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	204
Details	Domain: {"description":"Calponin-homology (CH)","evidences":[{"source":"PROSITE-ProRule","id":"PRU00044","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---