Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7SJ9

13pf E254A microtubule from recombinant human tubulin decorated with EB3

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000226	biological_process	microtubule cytoskeleton organization
A	0000278	biological_process	mitotic cell cycle
A	0003725	molecular_function	double-stranded RNA binding
A	0003924	molecular_function	GTPase activity
A	0005198	molecular_function	structural molecule activity
A	0005200	molecular_function	structural constituent of cytoskeleton
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005737	cellular_component	cytoplasm
A	0005856	cellular_component	cytoskeleton
A	0005874	cellular_component	microtubule
A	0005881	cellular_component	cytoplasmic microtubule
A	0005929	cellular_component	cilium
A	0007017	biological_process	microtubule-based process
A	0015630	cellular_component	microtubule cytoskeleton
A	0016787	molecular_function	hydrolase activity
A	0030182	biological_process	neuron differentiation
A	0030705	biological_process	cytoskeleton-dependent intracellular transport
A	0031625	molecular_function	ubiquitin protein ligase binding
A	0051301	biological_process	cell division
B	0000166	molecular_function	nucleotide binding
B	0000226	biological_process	microtubule cytoskeleton organization
B	0000278	biological_process	mitotic cell cycle
B	0003924	molecular_function	GTPase activity
B	0005200	molecular_function	structural constituent of cytoskeleton
B	0005515	molecular_function	protein binding
B	0005525	molecular_function	GTP binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005856	cellular_component	cytoskeleton
B	0005874	cellular_component	microtubule
B	0005929	cellular_component	cilium
B	0007017	biological_process	microtubule-based process
B	0007411	biological_process	axon guidance
B	0015630	cellular_component	microtubule cytoskeleton
B	0030027	cellular_component	lamellipodium
B	0030175	cellular_component	filopodium
B	0030182	biological_process	neuron differentiation
B	0030424	cellular_component	axon
B	0030425	cellular_component	dendrite
B	0030426	cellular_component	growth cone
B	0038007	biological_process	netrin-activated signaling pathway
B	0042277	molecular_function	peptide binding
B	0043025	cellular_component	neuronal cell body
B	0045171	cellular_component	intercellular bridge
B	0046872	molecular_function	metal ion binding
B	0070062	cellular_component	extracellular exosome
B	0071944	cellular_component	cell periphery
B	0072686	cellular_component	mitotic spindle
B	0097228	cellular_component	sperm principal piece
B	0097229	cellular_component	sperm end piece
B	0097542	cellular_component	ciliary tip
B	1990791	biological_process	dorsal root ganglion development
B	1990890	molecular_function	netrin receptor binding
C	0000166	molecular_function	nucleotide binding
C	0000226	biological_process	microtubule cytoskeleton organization
C	0000278	biological_process	mitotic cell cycle
C	0003725	molecular_function	double-stranded RNA binding
C	0003924	molecular_function	GTPase activity
C	0005198	molecular_function	structural molecule activity
C	0005200	molecular_function	structural constituent of cytoskeleton
C	0005515	molecular_function	protein binding
C	0005525	molecular_function	GTP binding
C	0005737	cellular_component	cytoplasm
C	0005856	cellular_component	cytoskeleton
C	0005874	cellular_component	microtubule
C	0005881	cellular_component	cytoplasmic microtubule
C	0005929	cellular_component	cilium
C	0007017	biological_process	microtubule-based process
C	0015630	cellular_component	microtubule cytoskeleton
C	0016787	molecular_function	hydrolase activity
C	0030182	biological_process	neuron differentiation
C	0030705	biological_process	cytoskeleton-dependent intracellular transport
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0051301	biological_process	cell division
D	0000166	molecular_function	nucleotide binding
D	0000226	biological_process	microtubule cytoskeleton organization
D	0000278	biological_process	mitotic cell cycle
D	0003924	molecular_function	GTPase activity
D	0005200	molecular_function	structural constituent of cytoskeleton
D	0005515	molecular_function	protein binding
D	0005525	molecular_function	GTP binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005856	cellular_component	cytoskeleton
D	0005874	cellular_component	microtubule
D	0005929	cellular_component	cilium
D	0007017	biological_process	microtubule-based process
D	0007411	biological_process	axon guidance
D	0015630	cellular_component	microtubule cytoskeleton
D	0030027	cellular_component	lamellipodium
D	0030175	cellular_component	filopodium
D	0030182	biological_process	neuron differentiation
D	0030424	cellular_component	axon
D	0030425	cellular_component	dendrite
D	0030426	cellular_component	growth cone
D	0038007	biological_process	netrin-activated signaling pathway
D	0042277	molecular_function	peptide binding
D	0043025	cellular_component	neuronal cell body
D	0045171	cellular_component	intercellular bridge
D	0046872	molecular_function	metal ion binding
D	0070062	cellular_component	extracellular exosome
D	0071944	cellular_component	cell periphery
D	0072686	cellular_component	mitotic spindle
D	0097228	cellular_component	sperm principal piece
D	0097229	cellular_component	sperm end piece
D	0097542	cellular_component	ciliary tip
D	1990791	biological_process	dorsal root ganglion development
D	1990890	molecular_function	netrin receptor binding
E	0000166	molecular_function	nucleotide binding
E	0000226	biological_process	microtubule cytoskeleton organization
E	0000278	biological_process	mitotic cell cycle
E	0003725	molecular_function	double-stranded RNA binding
E	0003924	molecular_function	GTPase activity
E	0005198	molecular_function	structural molecule activity
E	0005200	molecular_function	structural constituent of cytoskeleton
E	0005515	molecular_function	protein binding
E	0005525	molecular_function	GTP binding
E	0005737	cellular_component	cytoplasm
E	0005856	cellular_component	cytoskeleton
E	0005874	cellular_component	microtubule
E	0005881	cellular_component	cytoplasmic microtubule
E	0005929	cellular_component	cilium
E	0007017	biological_process	microtubule-based process
E	0015630	cellular_component	microtubule cytoskeleton
E	0016787	molecular_function	hydrolase activity
E	0030182	biological_process	neuron differentiation
E	0030705	biological_process	cytoskeleton-dependent intracellular transport
E	0031625	molecular_function	ubiquitin protein ligase binding
E	0051301	biological_process	cell division
F	0000166	molecular_function	nucleotide binding
F	0000226	biological_process	microtubule cytoskeleton organization
F	0000278	biological_process	mitotic cell cycle
F	0003924	molecular_function	GTPase activity
F	0005200	molecular_function	structural constituent of cytoskeleton
F	0005515	molecular_function	protein binding
F	0005525	molecular_function	GTP binding
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005856	cellular_component	cytoskeleton
F	0005874	cellular_component	microtubule
F	0005929	cellular_component	cilium
F	0007017	biological_process	microtubule-based process
F	0007411	biological_process	axon guidance
F	0015630	cellular_component	microtubule cytoskeleton
F	0030027	cellular_component	lamellipodium
F	0030175	cellular_component	filopodium
F	0030182	biological_process	neuron differentiation
F	0030424	cellular_component	axon
F	0030425	cellular_component	dendrite
F	0030426	cellular_component	growth cone
F	0038007	biological_process	netrin-activated signaling pathway
F	0042277	molecular_function	peptide binding
F	0043025	cellular_component	neuronal cell body
F	0045171	cellular_component	intercellular bridge
F	0046872	molecular_function	metal ion binding
F	0070062	cellular_component	extracellular exosome
F	0071944	cellular_component	cell periphery
F	0072686	cellular_component	mitotic spindle
F	0097228	cellular_component	sperm principal piece
F	0097229	cellular_component	sperm end piece
F	0097542	cellular_component	ciliary tip
F	1990791	biological_process	dorsal root ganglion development
F	1990890	molecular_function	netrin receptor binding
G	0000166	molecular_function	nucleotide binding
G	0000226	biological_process	microtubule cytoskeleton organization
G	0000278	biological_process	mitotic cell cycle
G	0003924	molecular_function	GTPase activity
G	0005200	molecular_function	structural constituent of cytoskeleton
G	0005515	molecular_function	protein binding
G	0005525	molecular_function	GTP binding
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0005856	cellular_component	cytoskeleton
G	0005874	cellular_component	microtubule
G	0005929	cellular_component	cilium
G	0007017	biological_process	microtubule-based process
G	0007411	biological_process	axon guidance
G	0015630	cellular_component	microtubule cytoskeleton
G	0030027	cellular_component	lamellipodium
G	0030175	cellular_component	filopodium
G	0030182	biological_process	neuron differentiation
G	0030424	cellular_component	axon
G	0030425	cellular_component	dendrite
G	0030426	cellular_component	growth cone
G	0038007	biological_process	netrin-activated signaling pathway
G	0042277	molecular_function	peptide binding
G	0043025	cellular_component	neuronal cell body
G	0045171	cellular_component	intercellular bridge
G	0046872	molecular_function	metal ion binding
G	0070062	cellular_component	extracellular exosome
G	0071944	cellular_component	cell periphery
G	0072686	cellular_component	mitotic spindle
G	0097228	cellular_component	sperm principal piece
G	0097229	cellular_component	sperm end piece
G	0097542	cellular_component	ciliary tip
G	1990791	biological_process	dorsal root ganglion development
G	1990890	molecular_function	netrin receptor binding
H	0000166	molecular_function	nucleotide binding
H	0000226	biological_process	microtubule cytoskeleton organization
H	0000278	biological_process	mitotic cell cycle
H	0003924	molecular_function	GTPase activity
H	0005200	molecular_function	structural constituent of cytoskeleton
H	0005515	molecular_function	protein binding
H	0005525	molecular_function	GTP binding
H	0005634	cellular_component	nucleus
H	0005737	cellular_component	cytoplasm
H	0005856	cellular_component	cytoskeleton
H	0005874	cellular_component	microtubule
H	0005929	cellular_component	cilium
H	0007017	biological_process	microtubule-based process
H	0007411	biological_process	axon guidance
H	0015630	cellular_component	microtubule cytoskeleton
H	0030027	cellular_component	lamellipodium
H	0030175	cellular_component	filopodium
H	0030182	biological_process	neuron differentiation
H	0030424	cellular_component	axon
H	0030425	cellular_component	dendrite
H	0030426	cellular_component	growth cone
H	0038007	biological_process	netrin-activated signaling pathway
H	0042277	molecular_function	peptide binding
H	0043025	cellular_component	neuronal cell body
H	0045171	cellular_component	intercellular bridge
H	0046872	molecular_function	metal ion binding
H	0070062	cellular_component	extracellular exosome
H	0071944	cellular_component	cell periphery
H	0072686	cellular_component	mitotic spindle
H	0097228	cellular_component	sperm principal piece
H	0097229	cellular_component	sperm end piece
H	0097542	cellular_component	ciliary tip
H	1990791	biological_process	dorsal root ganglion development
H	1990890	molecular_function	netrin receptor binding
I	0000166	molecular_function	nucleotide binding
I	0000226	biological_process	microtubule cytoskeleton organization
I	0000278	biological_process	mitotic cell cycle
I	0003924	molecular_function	GTPase activity
I	0005200	molecular_function	structural constituent of cytoskeleton
I	0005515	molecular_function	protein binding
I	0005525	molecular_function	GTP binding
I	0005634	cellular_component	nucleus
I	0005737	cellular_component	cytoplasm
I	0005856	cellular_component	cytoskeleton
I	0005874	cellular_component	microtubule
I	0005929	cellular_component	cilium
I	0007017	biological_process	microtubule-based process
I	0007411	biological_process	axon guidance
I	0015630	cellular_component	microtubule cytoskeleton
I	0030027	cellular_component	lamellipodium
I	0030175	cellular_component	filopodium
I	0030182	biological_process	neuron differentiation
I	0030424	cellular_component	axon
I	0030425	cellular_component	dendrite
I	0030426	cellular_component	growth cone
I	0038007	biological_process	netrin-activated signaling pathway
I	0042277	molecular_function	peptide binding
I	0043025	cellular_component	neuronal cell body
I	0045171	cellular_component	intercellular bridge
I	0046872	molecular_function	metal ion binding
I	0070062	cellular_component	extracellular exosome
I	0071944	cellular_component	cell periphery
I	0072686	cellular_component	mitotic spindle
I	0097228	cellular_component	sperm principal piece
I	0097229	cellular_component	sperm end piece
I	0097542	cellular_component	ciliary tip
I	1990791	biological_process	dorsal root ganglion development
I	1990890	molecular_function	netrin receptor binding
J	0000166	molecular_function	nucleotide binding
J	0000226	biological_process	microtubule cytoskeleton organization
J	0000278	biological_process	mitotic cell cycle
J	0003725	molecular_function	double-stranded RNA binding
J	0003924	molecular_function	GTPase activity
J	0005198	molecular_function	structural molecule activity
J	0005200	molecular_function	structural constituent of cytoskeleton
J	0005515	molecular_function	protein binding
J	0005525	molecular_function	GTP binding
J	0005737	cellular_component	cytoplasm
J	0005856	cellular_component	cytoskeleton
J	0005874	cellular_component	microtubule
J	0005881	cellular_component	cytoplasmic microtubule
J	0005929	cellular_component	cilium
J	0007017	biological_process	microtubule-based process
J	0015630	cellular_component	microtubule cytoskeleton
J	0016787	molecular_function	hydrolase activity
J	0030182	biological_process	neuron differentiation
J	0030705	biological_process	cytoskeleton-dependent intracellular transport
J	0031625	molecular_function	ubiquitin protein ligase binding
J	0051301	biological_process	cell division
K	0000166	molecular_function	nucleotide binding
K	0000226	biological_process	microtubule cytoskeleton organization
K	0000278	biological_process	mitotic cell cycle
K	0003725	molecular_function	double-stranded RNA binding
K	0003924	molecular_function	GTPase activity
K	0005198	molecular_function	structural molecule activity
K	0005200	molecular_function	structural constituent of cytoskeleton
K	0005515	molecular_function	protein binding
K	0005525	molecular_function	GTP binding
K	0005737	cellular_component	cytoplasm
K	0005856	cellular_component	cytoskeleton
K	0005874	cellular_component	microtubule
K	0005881	cellular_component	cytoplasmic microtubule
K	0005929	cellular_component	cilium
K	0007017	biological_process	microtubule-based process
K	0015630	cellular_component	microtubule cytoskeleton
K	0016787	molecular_function	hydrolase activity
K	0030182	biological_process	neuron differentiation
K	0030705	biological_process	cytoskeleton-dependent intracellular transport
K	0031625	molecular_function	ubiquitin protein ligase binding
K	0051301	biological_process	cell division
L	0000166	molecular_function	nucleotide binding
L	0000226	biological_process	microtubule cytoskeleton organization
L	0000278	biological_process	mitotic cell cycle
L	0003725	molecular_function	double-stranded RNA binding
L	0003924	molecular_function	GTPase activity
L	0005198	molecular_function	structural molecule activity
L	0005200	molecular_function	structural constituent of cytoskeleton
L	0005515	molecular_function	protein binding
L	0005525	molecular_function	GTP binding
L	0005737	cellular_component	cytoplasm
L	0005856	cellular_component	cytoskeleton
L	0005874	cellular_component	microtubule
L	0005881	cellular_component	cytoplasmic microtubule
L	0005929	cellular_component	cilium
L	0007017	biological_process	microtubule-based process
L	0015630	cellular_component	microtubule cytoskeleton
L	0016787	molecular_function	hydrolase activity
L	0030182	biological_process	neuron differentiation
L	0030705	biological_process	cytoskeleton-dependent intracellular transport
L	0031625	molecular_function	ubiquitin protein ligase binding
L	0051301	biological_process	cell division
M	0005515	molecular_function	protein binding
M	0005737	cellular_component	cytoplasm
M	0005815	cellular_component	microtubule organizing center
M	0005856	cellular_component	cytoskeleton
M	0005874	cellular_component	microtubule
M	0008017	molecular_function	microtubule binding
M	0008104	biological_process	intracellular protein localization
M	0015630	cellular_component	microtubule cytoskeleton
M	0030496	cellular_component	midbody
M	0031110	biological_process	regulation of microtubule polymerization or depolymerization
M	0031113	biological_process	regulation of microtubule polymerization
M	0035371	cellular_component	microtubule plus-end
M	0035372	biological_process	protein localization to microtubule
M	0042802	molecular_function	identical protein binding
M	0043539	molecular_function	protein serine/threonine kinase activator activity
M	0045893	biological_process	positive regulation of DNA-templated transcription
M	0048148	biological_process	behavioral response to cocaine
M	0051010	molecular_function	microtubule plus-end binding
M	0051225	biological_process	spindle assembly
M	0051233	cellular_component	spindle midzone
M	0051301	biological_process	cell division
M	0061003	biological_process	positive regulation of dendritic spine morphogenesis
M	0098984	cellular_component	neuron to neuron synapse
M	0120283	molecular_function	protein serine/threonine kinase binding
M	1905721	cellular_component	mitotic spindle astral microtubule end
N	0005515	molecular_function	protein binding
N	0005737	cellular_component	cytoplasm
N	0005815	cellular_component	microtubule organizing center
N	0005856	cellular_component	cytoskeleton
N	0005874	cellular_component	microtubule
N	0008017	molecular_function	microtubule binding
N	0008104	biological_process	intracellular protein localization
N	0015630	cellular_component	microtubule cytoskeleton
N	0030496	cellular_component	midbody
N	0031110	biological_process	regulation of microtubule polymerization or depolymerization
N	0031113	biological_process	regulation of microtubule polymerization
N	0035371	cellular_component	microtubule plus-end
N	0035372	biological_process	protein localization to microtubule
N	0042802	molecular_function	identical protein binding
N	0043539	molecular_function	protein serine/threonine kinase activator activity
N	0045893	biological_process	positive regulation of DNA-templated transcription
N	0048148	biological_process	behavioral response to cocaine
N	0051010	molecular_function	microtubule plus-end binding
N	0051225	biological_process	spindle assembly
N	0051233	cellular_component	spindle midzone
N	0051301	biological_process	cell division
N	0061003	biological_process	positive regulation of dendritic spine morphogenesis
N	0098984	cellular_component	neuron to neuron synapse
N	0120283	molecular_function	protein serine/threonine kinase binding
N	1905721	cellular_component	mitotic spindle astral microtubule end

Functional Information from PROSITE/UniProt

site_id	PS00227
Number of Residues	7
Details	TUBULIN Tubulin subunits alpha, beta, and gamma signature. GGGTGSG

Chain	Residue	Details
B	GLY140-GLY146
A	GLY142-GLY148

site_id	PS00228
Number of Residues	4
Details	TUBULIN_B_AUTOREG Tubulin-beta mRNA autoregulation signal. MREI

Chain	Residue	Details
B	MET1-ILE4

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	18
Details	Motif: {"description":"MREC motif","evidences":[{"source":"PubMed","id":"31727855","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	6
Details	Active site: {"evidences":[{"source":"PubMed","id":"34996871","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	90
Details	Binding site: {"evidences":[{"source":"PubMed","id":"38609661","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VT7","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	48
Details	Binding site: {"evidences":[{"source":"PubMed","id":"34996871","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"35482892","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38609661","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7SJ7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7Z6S","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VT7","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	12
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"MAR-2009","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Waridel P.","Quadroni M."]}}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	6
Details	Modified residue: {"description":"3'-nitrotyrosine","evidences":[{"source":"UniProtKB","id":"P68373","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	6
Details	Modified residue: {"description":"Omega-N-methylarginine","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"MAR-2009","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Waridel P.","Quadroni M."]}}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	6
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P68373","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	18
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)"}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	18
Details	Motif: {"description":"MREI motif","evidences":[{"source":"UniProtKB","id":"P07437","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	36
Details	Binding site: {"evidences":[{"source":"PubMed","id":"34996871","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"35482892","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"7SJ7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7Z6S","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P68363","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"34996871","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"7SJ7","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	6
Details	Modified residue: {"description":"Phosphoserine; by CDK1","evidences":[{"source":"PubMed","id":"16371510","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI15
Number of Residues	204
Details	Domain: {"description":"Calponin-homology (CH)","evidences":[{"source":"PROSITE-ProRule","id":"PRU00044","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)