7S7M
Complex of tissue inhibitor of metalloproteinases-1 (TIMP-1) mutant (L34G/M66D/T98G/P131S/Q153N) with matrix metalloproteinase-3 catalytic domain (MMP-3cd)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004222 | molecular_function | metalloendopeptidase activity |
| A | 0006508 | biological_process | proteolysis |
| A | 0008237 | molecular_function | metallopeptidase activity |
| A | 0008270 | molecular_function | zinc ion binding |
| A | 0031012 | cellular_component | extracellular matrix |
| B | 0002020 | molecular_function | protease binding |
| B | 0002248 | biological_process | connective tissue replacement involved in inflammatory response wound healing |
| B | 0005125 | molecular_function | cytokine activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005576 | cellular_component | extracellular region |
| B | 0005604 | cellular_component | basement membrane |
| B | 0005615 | cellular_component | extracellular space |
| B | 0005788 | cellular_component | endoplasmic reticulum lumen |
| B | 0007165 | biological_process | signal transduction |
| B | 0008083 | molecular_function | growth factor activity |
| B | 0008191 | molecular_function | metalloendopeptidase inhibitor activity |
| B | 0008270 | molecular_function | zinc ion binding |
| B | 0008284 | biological_process | positive regulation of cell population proliferation |
| B | 0009725 | biological_process | response to hormone |
| B | 0010466 | biological_process | negative regulation of peptidase activity |
| B | 0010951 | biological_process | negative regulation of endopeptidase activity |
| B | 0030414 | molecular_function | peptidase inhibitor activity |
| B | 0031012 | cellular_component | extracellular matrix |
| B | 0031093 | cellular_component | platelet alpha granule lumen |
| B | 0034097 | biological_process | response to cytokine |
| B | 0043066 | biological_process | negative regulation of apoptotic process |
| B | 0043086 | biological_process | negative regulation of catalytic activity |
| B | 0043434 | biological_process | response to peptide hormone |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0051045 | biological_process | negative regulation of membrane protein ectodomain proteolysis |
| B | 0051216 | biological_process | cartilage development |
| B | 0070062 | cellular_component | extracellular exosome |
| B | 0071492 | biological_process | cellular response to UV-A |
| B | 1901164 | biological_process | negative regulation of trophoblast cell migration |
| B | 1901653 | biological_process | cellular response to peptide |
| B | 1905049 | biological_process | negative regulation of metallopeptidase activity |
| B | 2001044 | biological_process | regulation of integrin-mediated signaling pathway |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | binding site for residue CA A 301 |
| Chain | Residue |
| A | ASP141 |
| A | GLY173 |
| A | ASN175 |
| A | ASP177 |
| A | HOH401 |
| A | HOH405 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | binding site for residue CA A 302 |
| Chain | Residue |
| A | HOH410 |
| A | ASP107 |
| A | ASP182 |
| A | GLU184 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | binding site for residue CA A 303 |
| Chain | Residue |
| A | ASP158 |
| A | GLY159 |
| A | GLY161 |
| A | VAL163 |
| A | ASP181 |
| A | GLU184 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | binding site for residue ZN A 304 |
| Chain | Residue |
| A | HIS201 |
| A | HIS205 |
| A | HIS211 |
| B | CYS1 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | binding site for residue ZN A 305 |
| Chain | Residue |
| A | HIS151 |
| A | ASP153 |
| A | HIS166 |
| A | HIS179 |
Functional Information from PROSITE/UniProt
| site_id | PS00142 |
| Number of Residues | 10 |
| Details | ZINC_PROTEASE Neutral zinc metallopeptidases, zinc-binding region signature. VAAHEIGHSL |
| Chain | Residue | Details |
| A | VAL198-LEU207 |
| site_id | PS00288 |
| Number of Residues | 13 |
| Details | TIMP Tissue inhibitors of metalloproteinases signature. CtCvPpHPQtaFC |
| Chain | Residue | Details |
| B | CYS1-CYS13 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:22427646 |
| Chain | Residue | Details |
| B | CYS1 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | SITE: Involved in metalloproteinase-binding => ECO:0000269|PubMed:22427646, ECO:0007744|PDB:3V96 |
| Chain | Residue | Details |
| B | GLY34 | |
| A | GLY173 | |
| A | ASN175 | |
| A | ASP177 | |
| A | HIS179 | |
| A | ASP181 | |
| A | ASP182 | |
| A | GLU184 | |
| B | ILE135 | |
| A | HIS151 | |
| A | ASP153 | |
| A | ASP158 | |
| A | GLY159 | |
| A | GLY161 | |
| A | VAL163 | |
| A | HIS166 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine; by FAM20C => ECO:0000269|PubMed:26091039 |
| Chain | Residue | Details |
| B | SER155 | |
| A | HIS205 | |
| A | HIS211 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | CARBOHYD: N-linked (GlcNAc...) (complex) asparagine => ECO:0000269|PubMed:16263699, ECO:0000269|PubMed:16335952, ECO:0000269|PubMed:16740002, ECO:0000269|PubMed:19139490 |
| Chain | Residue | Details |
| B | ASN30 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:16740002 |
| Chain | Residue | Details |
| B | ASN78 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 591 |
| Chain | Residue | Details |
| A | HIS201 | metal ligand |
| A | GLU202 | proton acceptor, proton donor |
| A | HIS205 | metal ligand |
| A | HIS211 | metal ligand |
