Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7Q5Y

Structure of NADH:ubichinon oxidoreductase (complex I) of the hyperthermophilic eubacterium Aquifex aeolicus

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
A	0016020	cellular_component	membrane
A	0016491	molecular_function	oxidoreductase activity
A	0042773	biological_process	ATP synthesis coupled electron transport
A	0051536	molecular_function	iron-sulfur cluster binding
A	1902600	biological_process	proton transmembrane transport
B	0005886	cellular_component	plasma membrane
B	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
B	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
B	0016655	molecular_function	oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
B	0030964	cellular_component	NADH dehydrogenase complex
B	0048038	molecular_function	quinone binding
B	0050136	molecular_function	NADH dehydrogenase (quinone) (non-electrogenic) activity
B	0051287	molecular_function	NAD binding
B	1902600	biological_process	proton transmembrane transport
C	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
C	0010181	molecular_function	FMN binding
C	0051539	molecular_function	4 iron, 4 sulfur cluster binding
C	1902600	biological_process	proton transmembrane transport
D	0005506	molecular_function	iron ion binding
D	0005886	cellular_component	plasma membrane
D	0016020	cellular_component	membrane
D	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
D	0050136	molecular_function	NADH dehydrogenase (quinone) (non-electrogenic) activity
D	0051539	molecular_function	4 iron, 4 sulfur cluster binding
E	0003954	molecular_function	NADH dehydrogenase activity
E	0016491	molecular_function	oxidoreductase activity
E	0022904	biological_process	respiratory electron transport chain
E	0030964	cellular_component	NADH dehydrogenase complex
E	0051537	molecular_function	2 iron, 2 sulfur cluster binding
F	0005506	molecular_function	iron ion binding
F	0005886	cellular_component	plasma membrane
F	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
F	0009060	biological_process	aerobic respiration
F	0015990	biological_process	electron transport coupled proton transport
F	0045271	cellular_component	respiratory chain complex I
F	0048038	molecular_function	quinone binding
F	0050136	molecular_function	NADH dehydrogenase (quinone) (non-electrogenic) activity
F	0051536	molecular_function	iron-sulfur cluster binding
F	0051539	molecular_function	4 iron, 4 sulfur cluster binding
G	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
G	0016020	cellular_component	membrane
G	0016491	molecular_function	oxidoreductase activity
G	0042773	biological_process	ATP synthesis coupled electron transport
G	0051536	molecular_function	iron-sulfur cluster binding
G	1902600	biological_process	proton transmembrane transport
H	0005886	cellular_component	plasma membrane
H	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
H	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
H	0016655	molecular_function	oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
H	0030964	cellular_component	NADH dehydrogenase complex
H	0048038	molecular_function	quinone binding
H	0050136	molecular_function	NADH dehydrogenase (quinone) (non-electrogenic) activity
H	0051287	molecular_function	NAD binding
H	1902600	biological_process	proton transmembrane transport
I	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
I	0010181	molecular_function	FMN binding
I	0051539	molecular_function	4 iron, 4 sulfur cluster binding
I	1902600	biological_process	proton transmembrane transport
J	0005506	molecular_function	iron ion binding
J	0005886	cellular_component	plasma membrane
J	0016020	cellular_component	membrane
J	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
J	0050136	molecular_function	NADH dehydrogenase (quinone) (non-electrogenic) activity
J	0051539	molecular_function	4 iron, 4 sulfur cluster binding
K	0003954	molecular_function	NADH dehydrogenase activity
K	0016491	molecular_function	oxidoreductase activity
K	0022904	biological_process	respiratory electron transport chain
K	0030964	cellular_component	NADH dehydrogenase complex
K	0051537	molecular_function	2 iron, 2 sulfur cluster binding
L	0005506	molecular_function	iron ion binding
L	0005886	cellular_component	plasma membrane
L	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
L	0009060	biological_process	aerobic respiration
L	0015990	biological_process	electron transport coupled proton transport
L	0045271	cellular_component	respiratory chain complex I
L	0048038	molecular_function	quinone binding
L	0050136	molecular_function	NADH dehydrogenase (quinone) (non-electrogenic) activity
L	0051536	molecular_function	iron-sulfur cluster binding
L	0051539	molecular_function	4 iron, 4 sulfur cluster binding
M	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
M	0016020	cellular_component	membrane
M	0016491	molecular_function	oxidoreductase activity
M	0042773	biological_process	ATP synthesis coupled electron transport
M	0051536	molecular_function	iron-sulfur cluster binding
M	1902600	biological_process	proton transmembrane transport
N	0005886	cellular_component	plasma membrane
N	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
N	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
N	0016655	molecular_function	oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
N	0030964	cellular_component	NADH dehydrogenase complex
N	0048038	molecular_function	quinone binding
N	0050136	molecular_function	NADH dehydrogenase (quinone) (non-electrogenic) activity
N	0051287	molecular_function	NAD binding
N	1902600	biological_process	proton transmembrane transport
O	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
O	0010181	molecular_function	FMN binding
O	0051539	molecular_function	4 iron, 4 sulfur cluster binding
O	1902600	biological_process	proton transmembrane transport
P	0005506	molecular_function	iron ion binding
P	0005886	cellular_component	plasma membrane
P	0016020	cellular_component	membrane
P	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
P	0050136	molecular_function	NADH dehydrogenase (quinone) (non-electrogenic) activity
P	0051539	molecular_function	4 iron, 4 sulfur cluster binding
Q	0003954	molecular_function	NADH dehydrogenase activity
Q	0016491	molecular_function	oxidoreductase activity
Q	0022904	biological_process	respiratory electron transport chain
Q	0030964	cellular_component	NADH dehydrogenase complex
Q	0051537	molecular_function	2 iron, 2 sulfur cluster binding
R	0005506	molecular_function	iron ion binding
R	0005886	cellular_component	plasma membrane
R	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
R	0009060	biological_process	aerobic respiration
R	0015990	biological_process	electron transport coupled proton transport
R	0045271	cellular_component	respiratory chain complex I
R	0048038	molecular_function	quinone binding
R	0050136	molecular_function	NADH dehydrogenase (quinone) (non-electrogenic) activity
R	0051536	molecular_function	iron-sulfur cluster binding
R	0051539	molecular_function	4 iron, 4 sulfur cluster binding
S	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
S	0016020	cellular_component	membrane
S	0016491	molecular_function	oxidoreductase activity
S	0042773	biological_process	ATP synthesis coupled electron transport
S	0051536	molecular_function	iron-sulfur cluster binding
S	1902600	biological_process	proton transmembrane transport
T	0005886	cellular_component	plasma membrane
T	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
T	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
T	0016655	molecular_function	oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
T	0030964	cellular_component	NADH dehydrogenase complex
T	0048038	molecular_function	quinone binding
T	0050136	molecular_function	NADH dehydrogenase (quinone) (non-electrogenic) activity
T	0051287	molecular_function	NAD binding
T	1902600	biological_process	proton transmembrane transport
U	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
U	0010181	molecular_function	FMN binding
U	0051539	molecular_function	4 iron, 4 sulfur cluster binding
U	1902600	biological_process	proton transmembrane transport
V	0005506	molecular_function	iron ion binding
V	0005886	cellular_component	plasma membrane
V	0016020	cellular_component	membrane
V	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
V	0050136	molecular_function	NADH dehydrogenase (quinone) (non-electrogenic) activity
V	0051539	molecular_function	4 iron, 4 sulfur cluster binding
W	0003954	molecular_function	NADH dehydrogenase activity
W	0016491	molecular_function	oxidoreductase activity
W	0022904	biological_process	respiratory electron transport chain
W	0030964	cellular_component	NADH dehydrogenase complex
W	0051537	molecular_function	2 iron, 2 sulfur cluster binding
X	0005506	molecular_function	iron ion binding
X	0005886	cellular_component	plasma membrane
X	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
X	0009060	biological_process	aerobic respiration
X	0015990	biological_process	electron transport coupled proton transport
X	0045271	cellular_component	respiratory chain complex I
X	0048038	molecular_function	quinone binding
X	0050136	molecular_function	NADH dehydrogenase (quinone) (non-electrogenic) activity
X	0051536	molecular_function	iron-sulfur cluster binding
X	0051539	molecular_function	4 iron, 4 sulfur cluster binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	8
Details	binding site for residue SF4 A 701

Chain	Residue
A	HIS99
A	ASP102
A	CYS103
A	CYS106
A	ALA109
A	CYS112
A	GLN115
A	GLY209

site_id	AC2
Number of Residues	9
Details	binding site for residue SF4 A 702

Chain	Residue
A	CYS154
A	VAL155
A	CYS157
A	TYR158
A	CYS160
A	CYS206
A	ALA210
A	ILE211
A	TYR149

site_id	AC3
Number of Residues	7
Details	binding site for residue SF4 A 703

Chain	Residue
A	CYS232
A	LEU234
A	CYS235
A	GLY238
A	CYS239
A	CYS268
A	VAL385

site_id	AC4
Number of Residues	8
Details	binding site for residue SF4 A 704

Chain	Residue
A	CYS164
A	THR170
A	MET191
A	CYS196
A	GLU197
A	CYS199
A	GLY200
A	CYS202

site_id	AC5
Number of Residues	7
Details	binding site for residue FES A 705

Chain	Residue
A	CYS37
A	TYR38
A	GLY46
A	CYS48
A	ARG49
A	CYS51
A	CYS65

site_id	AC6
Number of Residues	16
Details	binding site for residue FMN C 501

Chain	Residue
C	GLY65
C	ARG66
C	GLY67
C	LYS76
C	ASN92
C	GLU95
C	TYR180
C	GLY183
C	GLU184
C	GLU185
C	VAL218
C	ASN219
C	ASN220
C	THR223
C	GLY394
C	HOH616

site_id	AC7
Number of Residues	9
Details	binding site for residue SF4 C 502

Chain	Residue
C	THR346
C	CYS347
C	GLY348
C	GLN349
C	CYS350
C	CYS353
C	SER391
C	CYS393
C	GLY396

site_id	AC8
Number of Residues	9
Details	binding site for residue SF4 D 301

Chain	Residue
D	HIS58
D	CYS97
D	PHE103
D	CYS127
D	LEU128
D	PHE129
D	CYS130
D	GLY131
D	CYS133

site_id	AC9
Number of Residues	7
Details	binding site for residue SF4 D 302

Chain	Residue
D	CYS87
D	VAL88
D	ALA89
D	CYS90
D	TYR91
D	CYS93
D	CYS137

site_id	AD1
Number of Residues	9
Details	binding site for residue FES E 201

Chain	Residue
E	CYS86
E	SER88
E	VAL90
E	CYS91
E	CYS127
E	LEU128
E	GLY129
E	ALA130
E	CYS131

site_id	AD2
Number of Residues	7
Details	binding site for residue SF4 F 701

Chain	Residue
F	ALA34
F	CYS35
F	CYS36
F	GLY99
F	CYS100
F	CYS129
F	PRO130

site_id	AD3
Number of Residues	9
Details	binding site for residue SF4 G 701

Chain	Residue
G	CYS106
G	ALA109
G	CYS112
G	GLN115
G	VAL208
G	GLY209
G	HIS99
G	ASP102
G	CYS103

site_id	AD4
Number of Residues	9
Details	binding site for residue SF4 G 702

Chain	Residue
G	TYR149
G	CYS154
G	VAL155
G	CYS157
G	TYR158
G	CYS160
G	CYS206
G	ALA210
G	ILE211

site_id	AD5
Number of Residues	7
Details	binding site for residue SF4 G 703

Chain	Residue
G	CYS232
G	LEU234
G	CYS235
G	GLY238
G	CYS239
G	CYS268
G	VAL385

site_id	AD6
Number of Residues	7
Details	binding site for residue SF4 G 704

Chain	Residue
G	CYS164
G	THR170
G	CYS196
G	GLU197
G	CYS199
G	GLY200
G	CYS202

site_id	AD7
Number of Residues	8
Details	binding site for residue FES G 705

Chain	Residue
G	TYR35
G	CYS37
G	TYR38
G	GLY46
G	CYS48
G	ARG49
G	CYS51
G	CYS65

site_id	AD8
Number of Residues	17
Details	binding site for residue FMN I 501

Chain	Residue
I	GLY65
I	ARG66
I	GLY67
I	LYS76
I	ASN92
I	GLU95
I	TYR180
I	GLY183
I	GLU184
I	GLU185
I	VAL218
I	ASN219
I	ASN220
I	THR223
I	HOH614
I	HOH615
I	HOH616

site_id	AD9
Number of Residues	9
Details	binding site for residue SF4 I 502

Chain	Residue
I	ILE181
I	THR346
I	CYS347
I	GLY348
I	GLN349
I	CYS350
I	CYS353
I	CYS393
I	GLY396

site_id	AE1
Number of Residues	9
Details	binding site for residue SF4 J 301

Chain	Residue
J	HIS58
J	CYS97
J	PHE103
J	CYS127
J	LEU128
J	PHE129
J	CYS130
J	GLY131
J	CYS133

site_id	AE2
Number of Residues	7
Details	binding site for residue SF4 J 302

Chain	Residue
J	CYS87
J	VAL88
J	ALA89
J	CYS90
J	TYR91
J	CYS93
J	CYS137

site_id	AE3
Number of Residues	9
Details	binding site for residue FES K 201

Chain	Residue
K	CYS86
K	SER88
K	VAL90
K	CYS91
K	CYS127
K	LEU128
K	GLY129
K	ALA130
K	CYS131

site_id	AE4
Number of Residues	9
Details	binding site for residue SF4 L 701

Chain	Residue
H	ARG257
L	ALA34
L	CYS35
L	CYS36
L	GLY99
L	CYS100
L	GLY128
L	CYS129
L	PRO130

site_id	AE5
Number of Residues	9
Details	binding site for residue SF4 M 701

Chain	Residue
M	HIS99
M	ASP102
M	CYS103
M	CYS106
M	ALA109
M	CYS112
M	GLN115
M	VAL208
M	GLY209

site_id	AE6
Number of Residues	8
Details	binding site for residue SF4 M 702

Chain	Residue
M	TYR149
M	CYS154
M	VAL155
M	CYS157
M	TYR158
M	CYS160
M	CYS206
M	ILE211

site_id	AE7
Number of Residues	7
Details	binding site for residue SF4 M 703

Chain	Residue
M	CYS232
M	LEU234
M	CYS235
M	GLY238
M	CYS239
M	CYS268
M	VAL385

site_id	AE8
Number of Residues	9
Details	binding site for residue SF4 M 704

Chain	Residue
M	CYS164
M	THR170
M	MET191
M	CYS196
M	GLU197
M	MET198
M	CYS199
M	GLY200
M	CYS202

site_id	AE9
Number of Residues	8
Details	binding site for residue FES M 705

Chain	Residue
M	TYR35
M	CYS37
M	TYR38
M	GLY46
M	CYS48
M	ARG49
M	CYS51
M	CYS65

site_id	AF1
Number of Residues	17
Details	binding site for residue FMN O 501

Chain	Residue
O	GLY65
O	ARG66
O	GLY67
O	LYS76
O	ASN92
O	ASP94
O	GLU95
O	TYR180
O	GLY183
O	GLU184
O	GLU185
O	VAL218
O	ASN219
O	ASN220
O	THR223
O	GLY394
O	HOH602

site_id	AF2
Number of Residues	8
Details	binding site for residue SF4 O 502

Chain	Residue
O	THR346
O	CYS347
O	GLY348
O	GLN349
O	CYS350
O	CYS353
O	CYS393
O	GLY396

site_id	AF3
Number of Residues	9
Details	binding site for residue SF4 P 301

Chain	Residue
P	HIS58
P	CYS97
P	PHE103
P	CYS127
P	LEU128
P	PHE129
P	CYS130
P	GLY131
P	CYS133

site_id	AF4
Number of Residues	8
Details	binding site for residue SF4 P 302

Chain	Residue
P	CYS87
P	VAL88
P	ALA89
P	CYS90
P	TYR91
P	CYS93
P	PHE120
P	CYS137

site_id	AF5
Number of Residues	9
Details	binding site for residue FES Q 201

Chain	Residue
Q	CYS86
Q	SER88
Q	VAL90
Q	CYS91
Q	CYS127
Q	LEU128
Q	GLY129
Q	ALA130
Q	CYS131

site_id	AF6
Number of Residues	8
Details	binding site for residue SF4 R 701

Chain	Residue
R	ALA34
R	CYS35
R	CYS36
R	GLY71
R	GLY99
R	CYS100
R	CYS129
R	PRO130

site_id	AF7
Number of Residues	7
Details	binding site for residue SF4 S 701

Chain	Residue
S	HIS99
S	ASP102
S	CYS103
S	CYS106
S	ALA109
S	CYS112
S	GLY209

site_id	AF8
Number of Residues	8
Details	binding site for residue SF4 S 702

Chain	Residue
S	TYR149
S	CYS154
S	VAL155
S	CYS157
S	TYR158
S	CYS160
S	CYS206
S	ILE211

site_id	AF9
Number of Residues	7
Details	binding site for residue SF4 S 703

Chain	Residue
S	CYS232
S	LEU234
S	CYS235
S	GLY238
S	CYS239
S	CYS268
S	VAL385

site_id	AG1
Number of Residues	8
Details	binding site for residue SF4 S 704

Chain	Residue
S	CYS164
S	THR170
S	MET191
S	CYS196
S	GLU197
S	CYS199
S	GLY200
S	CYS202

site_id	AG2
Number of Residues	8
Details	binding site for residue FES S 705

Chain	Residue
S	TYR35
S	CYS37
S	TYR38
S	GLY46
S	CYS48
S	ARG49
S	CYS51
S	CYS65

site_id	AG3
Number of Residues	16
Details	binding site for residue FMN U 501

Chain	Residue
U	GLY65
U	ARG66
U	GLY67
U	LYS76
U	ASN92
U	ASP94
U	GLU95
U	TYR180
U	GLY183
U	GLU184
U	GLU185
U	VAL218
U	ASN219
U	ASN220
U	THR223
U	HOH604

site_id	AG4
Number of Residues	9
Details	binding site for residue SF4 U 502

Chain	Residue
U	ILE181
U	THR346
U	CYS347
U	GLY348
U	GLN349
U	CYS350
U	CYS353
U	CYS393
U	GLY396

site_id	AG5
Number of Residues	10
Details	binding site for residue SF4 V 301

Chain	Residue
V	HIS58
V	CYS97
V	PHE103
V	MET122
V	CYS127
V	LEU128
V	PHE129
V	CYS130
V	GLY131
V	CYS133

site_id	AG6
Number of Residues	7
Details	binding site for residue SF4 V 302

Chain	Residue
V	CYS87
V	VAL88
V	ALA89
V	CYS90
V	TYR91
V	CYS93
V	CYS137

site_id	AG7
Number of Residues	9
Details	binding site for residue FES W 201

Chain	Residue
W	CYS86
W	SER88
W	VAL90
W	CYS91
W	CYS127
W	LEU128
W	GLY129
W	ALA130
W	CYS131

site_id	AG8
Number of Residues	10
Details	binding site for residue SF4 X 701

Chain	Residue
T	ARG257
X	ALA34
X	CYS35
X	CYS36
X	GLY71
X	GLY99
X	CYS100
X	PHE107
X	CYS129
X	PRO130

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGFGGTGTVIvLteeddive..........AALK

Chain	Residue	Details
C	LEU314-LYS337

site_id	PS00198
Number of Residues	12
Details	4FE4S_FER_1 4Fe-4S ferredoxin-type iron-sulfur binding region signature. CeMCGiCVhVCP

Chain	Residue	Details
A	CYS196-PRO207
D	CYS87-PRO98
D	CYS127-PRO138

site_id	PS00641
Number of Residues	18
Details	COMPLEX1_75K_1 Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 1. PyfCYhprlsiaGaCRmC

Chain	Residue	Details
A	PRO34-CYS51

site_id	PS00644
Number of Residues	16
Details	COMPLEX1_51K_1 Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 1. GAGAYICGEETALIES

Chain	Residue	Details
C	GLY176-SER191

site_id	PS00645
Number of Residues	12
Details	COMPLEX1_51K_2 Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 2. ETCGqCtPCRvG

Chain	Residue	Details
C	GLU345-GLY356

site_id	PS01099
Number of Residues	19
Details	COMPLEX1_24K Respiratory-chain NADH dehydrogenase 24 Kd subunit signature. DgkFKivpvqCLGaCseAP

Chain	Residue	Details
E	ASP117-PRO135

site_id	PS01150
Number of Residues	17
Details	COMPLEX1_20K Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. GvDRIIPVDVYiPgCPP

Chain	Residue	Details
F	GLY115-PRO131

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI5
Number of Residues	688
Details	Region: {"description":"NADH dehydrogenase I subunit C","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	224
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	32
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	116
Details	Domain: {"description":"4Fe-4S ferredoxin-type 1","evidences":[{"source":"HAMAP-Rule","id":"MF_01351","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	124
Details	Domain: {"description":"4Fe-4S ferredoxin-type 2","evidences":[{"source":"HAMAP-Rule","id":"MF_01351","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details