Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7Q57

Single Particle Cryo-EM structure of photosynthetic A10B10 glyceraldehyde-3-phospahte dehydrogenase from Spinacia oleracea.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
A	0006006	biological_process	glucose metabolic process
A	0009507	cellular_component	chloroplast
A	0016491	molecular_function	oxidoreductase activity
A	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
A	0019253	biological_process	reductive pentose-phosphate cycle
A	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
A	0050661	molecular_function	NADP binding
A	0051287	molecular_function	NAD binding
B	0006006	biological_process	glucose metabolic process
B	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
B	0050661	molecular_function	NADP binding
B	0051287	molecular_function	NAD binding
C	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
C	0006006	biological_process	glucose metabolic process
C	0009507	cellular_component	chloroplast
C	0016491	molecular_function	oxidoreductase activity
C	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
C	0019253	biological_process	reductive pentose-phosphate cycle
C	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
C	0050661	molecular_function	NADP binding
C	0051287	molecular_function	NAD binding
D	0006006	biological_process	glucose metabolic process
D	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
D	0050661	molecular_function	NADP binding
D	0051287	molecular_function	NAD binding
E	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
E	0006006	biological_process	glucose metabolic process
E	0009507	cellular_component	chloroplast
E	0016491	molecular_function	oxidoreductase activity
E	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
E	0019253	biological_process	reductive pentose-phosphate cycle
E	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
E	0050661	molecular_function	NADP binding
E	0051287	molecular_function	NAD binding
F	0006006	biological_process	glucose metabolic process
F	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
F	0050661	molecular_function	NADP binding
F	0051287	molecular_function	NAD binding
G	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
G	0006006	biological_process	glucose metabolic process
G	0009507	cellular_component	chloroplast
G	0016491	molecular_function	oxidoreductase activity
G	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
G	0019253	biological_process	reductive pentose-phosphate cycle
G	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
G	0050661	molecular_function	NADP binding
G	0051287	molecular_function	NAD binding
H	0006006	biological_process	glucose metabolic process
H	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
H	0050661	molecular_function	NADP binding
H	0051287	molecular_function	NAD binding
I	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
I	0006006	biological_process	glucose metabolic process
I	0009507	cellular_component	chloroplast
I	0016491	molecular_function	oxidoreductase activity
I	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
I	0019253	biological_process	reductive pentose-phosphate cycle
I	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
I	0050661	molecular_function	NADP binding
I	0051287	molecular_function	NAD binding
J	0006006	biological_process	glucose metabolic process
J	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
J	0050661	molecular_function	NADP binding
J	0051287	molecular_function	NAD binding
K	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
K	0006006	biological_process	glucose metabolic process
K	0009507	cellular_component	chloroplast
K	0016491	molecular_function	oxidoreductase activity
K	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
K	0019253	biological_process	reductive pentose-phosphate cycle
K	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
K	0050661	molecular_function	NADP binding
K	0051287	molecular_function	NAD binding
L	0006006	biological_process	glucose metabolic process
L	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
L	0050661	molecular_function	NADP binding
L	0051287	molecular_function	NAD binding
M	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
M	0006006	biological_process	glucose metabolic process
M	0009507	cellular_component	chloroplast
M	0016491	molecular_function	oxidoreductase activity
M	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
M	0019253	biological_process	reductive pentose-phosphate cycle
M	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
M	0050661	molecular_function	NADP binding
M	0051287	molecular_function	NAD binding
N	0006006	biological_process	glucose metabolic process
N	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
N	0050661	molecular_function	NADP binding
N	0051287	molecular_function	NAD binding
O	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
O	0006006	biological_process	glucose metabolic process
O	0009507	cellular_component	chloroplast
O	0016491	molecular_function	oxidoreductase activity
O	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
O	0019253	biological_process	reductive pentose-phosphate cycle
O	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
O	0050661	molecular_function	NADP binding
O	0051287	molecular_function	NAD binding
P	0006006	biological_process	glucose metabolic process
P	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
P	0050661	molecular_function	NADP binding
P	0051287	molecular_function	NAD binding
Q	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
Q	0006006	biological_process	glucose metabolic process
Q	0009507	cellular_component	chloroplast
Q	0016491	molecular_function	oxidoreductase activity
Q	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
Q	0019253	biological_process	reductive pentose-phosphate cycle
Q	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
Q	0050661	molecular_function	NADP binding
Q	0051287	molecular_function	NAD binding
R	0006006	biological_process	glucose metabolic process
R	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
R	0050661	molecular_function	NADP binding
R	0051287	molecular_function	NAD binding
S	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
S	0006006	biological_process	glucose metabolic process
S	0009507	cellular_component	chloroplast
S	0016491	molecular_function	oxidoreductase activity
S	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
S	0019253	biological_process	reductive pentose-phosphate cycle
S	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
S	0050661	molecular_function	NADP binding
S	0051287	molecular_function	NAD binding
T	0006006	biological_process	glucose metabolic process
T	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
T	0050661	molecular_function	NADP binding
T	0051287	molecular_function	NAD binding

Functional Information from PROSITE/UniProt

site_id	PS00071
Number of Residues	8
Details	GAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL

Chain	Residue	Details
P	ALA147-LEU154
O	ALA147-LEU154

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	10
Details	ACT_SITE: Nucleophile

Chain	Residue	Details
P	CYS149
R	CYS149
B	CYS149
D	CYS149
H	CYS149
F	CYS149
J	CYS149
L	CYS149
N	CYS149
T	CYS149

site_id	SWS_FT_FI2
Number of Residues	40
Details	BINDING: BINDING => ECO:0000269\|PubMed:11846565, ECO:0000269\|PubMed:12705826, ECO:0000269\|PubMed:15236965, ECO:0000269\|PubMed:17573533

Chain	Residue	Details
P	ARG10
P	ASP32
P	ARG77
P	ASN313
R	ARG10
R	ASP32
R	ARG77
R	ASN313
B	ARG10
B	ASP32
B	ARG77
B	ASN313
D	ARG10
D	ASP32
D	ARG77
D	ASN313
H	ARG10
H	ASP32
H	ARG77
H	ASN313
F	ARG10
F	ASP32
F	ARG77
F	ASN313
J	ARG10
J	ASP32
J	ARG77
J	ASN313
L	ARG10
L	ASP32
L	ARG77
L	ASN313
N	ARG10
N	ASP32
N	ARG77
N	ASN313
T	ARG10
T	ASP32
T	ARG77
T	ASN313

site_id	SWS_FT_FI3
Number of Residues	50
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
P	SER148
P	THR179
P	ARG195
P	THR208
P	ARG231
R	SER148
R	THR179
R	ARG195
R	THR208
R	ARG231
B	SER148
B	THR179
B	ARG195
B	THR208
B	ARG231
D	SER148
D	THR179
D	ARG195
D	THR208
D	ARG231
H	SER148
H	THR179
H	ARG195
H	THR208
H	ARG231
F	SER148
F	THR179
F	ARG195
F	THR208
F	ARG231
J	SER148
J	THR179
J	ARG195
J	THR208
J	ARG231
L	SER148
L	THR179
L	ARG195
L	THR208
L	ARG231
N	SER148
N	THR179
N	ARG195
N	THR208
N	ARG231
T	SER148
T	THR179
T	ARG195
T	THR208
T	ARG231

site_id	SWS_FT_FI4
Number of Residues	10
Details	SITE: Activates thiol group during catalysis => ECO:0000250

Chain	Residue	Details
P	HIS176
R	HIS176
B	HIS176
D	HIS176
H	HIS176
F	HIS176
J	HIS176
L	HIS176
N	HIS176
T	HIS176

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)