Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7Q55

Single Particle Cryo-EM structure of photosynthetic A8B8 glyceraldehyde-3-phosphate dehydrogenase hexadecamer (major conformer) from Spinacia oleracia.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
A	0006006	biological_process	glucose metabolic process
A	0009507	cellular_component	chloroplast
A	0016491	molecular_function	oxidoreductase activity
A	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
A	0019253	biological_process	reductive pentose-phosphate cycle
A	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
A	0050661	molecular_function	NADP binding
A	0051287	molecular_function	NAD binding
B	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
B	0006006	biological_process	glucose metabolic process
B	0009507	cellular_component	chloroplast
B	0016491	molecular_function	oxidoreductase activity
B	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
B	0019253	biological_process	reductive pentose-phosphate cycle
B	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
B	0050661	molecular_function	NADP binding
B	0051287	molecular_function	NAD binding
C	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
C	0006006	biological_process	glucose metabolic process
C	0009507	cellular_component	chloroplast
C	0016491	molecular_function	oxidoreductase activity
C	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
C	0019253	biological_process	reductive pentose-phosphate cycle
C	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
C	0050661	molecular_function	NADP binding
C	0051287	molecular_function	NAD binding
D	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
D	0006006	biological_process	glucose metabolic process
D	0009507	cellular_component	chloroplast
D	0016491	molecular_function	oxidoreductase activity
D	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
D	0019253	biological_process	reductive pentose-phosphate cycle
D	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
D	0050661	molecular_function	NADP binding
D	0051287	molecular_function	NAD binding
E	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
E	0006006	biological_process	glucose metabolic process
E	0009507	cellular_component	chloroplast
E	0016491	molecular_function	oxidoreductase activity
E	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
E	0019253	biological_process	reductive pentose-phosphate cycle
E	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
E	0050661	molecular_function	NADP binding
E	0051287	molecular_function	NAD binding
F	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
F	0006006	biological_process	glucose metabolic process
F	0009507	cellular_component	chloroplast
F	0016491	molecular_function	oxidoreductase activity
F	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
F	0019253	biological_process	reductive pentose-phosphate cycle
F	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
F	0050661	molecular_function	NADP binding
F	0051287	molecular_function	NAD binding
G	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
G	0006006	biological_process	glucose metabolic process
G	0009507	cellular_component	chloroplast
G	0016491	molecular_function	oxidoreductase activity
G	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
G	0019253	biological_process	reductive pentose-phosphate cycle
G	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
G	0050661	molecular_function	NADP binding
G	0051287	molecular_function	NAD binding
H	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
H	0006006	biological_process	glucose metabolic process
H	0009507	cellular_component	chloroplast
H	0016491	molecular_function	oxidoreductase activity
H	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
H	0019253	biological_process	reductive pentose-phosphate cycle
H	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
H	0050661	molecular_function	NADP binding
H	0051287	molecular_function	NAD binding
I	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
I	0006006	biological_process	glucose metabolic process
I	0009507	cellular_component	chloroplast
I	0016491	molecular_function	oxidoreductase activity
I	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
I	0019253	biological_process	reductive pentose-phosphate cycle
I	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
I	0050661	molecular_function	NADP binding
I	0051287	molecular_function	NAD binding
J	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
J	0006006	biological_process	glucose metabolic process
J	0009507	cellular_component	chloroplast
J	0016491	molecular_function	oxidoreductase activity
J	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
J	0019253	biological_process	reductive pentose-phosphate cycle
J	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
J	0050661	molecular_function	NADP binding
J	0051287	molecular_function	NAD binding
K	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
K	0006006	biological_process	glucose metabolic process
K	0009507	cellular_component	chloroplast
K	0016491	molecular_function	oxidoreductase activity
K	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
K	0019253	biological_process	reductive pentose-phosphate cycle
K	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
K	0050661	molecular_function	NADP binding
K	0051287	molecular_function	NAD binding
L	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
L	0006006	biological_process	glucose metabolic process
L	0009507	cellular_component	chloroplast
L	0016491	molecular_function	oxidoreductase activity
L	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
L	0019253	biological_process	reductive pentose-phosphate cycle
L	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
L	0050661	molecular_function	NADP binding
L	0051287	molecular_function	NAD binding
O	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
O	0006006	biological_process	glucose metabolic process
O	0009507	cellular_component	chloroplast
O	0016491	molecular_function	oxidoreductase activity
O	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
O	0019253	biological_process	reductive pentose-phosphate cycle
O	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
O	0050661	molecular_function	NADP binding
O	0051287	molecular_function	NAD binding
P	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
P	0006006	biological_process	glucose metabolic process
P	0009507	cellular_component	chloroplast
P	0016491	molecular_function	oxidoreductase activity
P	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
P	0019253	biological_process	reductive pentose-phosphate cycle
P	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
P	0050661	molecular_function	NADP binding
P	0051287	molecular_function	NAD binding
Q	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
Q	0006006	biological_process	glucose metabolic process
Q	0009507	cellular_component	chloroplast
Q	0016491	molecular_function	oxidoreductase activity
Q	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
Q	0019253	biological_process	reductive pentose-phosphate cycle
Q	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
Q	0050661	molecular_function	NADP binding
Q	0051287	molecular_function	NAD binding
R	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
R	0006006	biological_process	glucose metabolic process
R	0009507	cellular_component	chloroplast
R	0016491	molecular_function	oxidoreductase activity
R	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
R	0019253	biological_process	reductive pentose-phosphate cycle
R	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
R	0050661	molecular_function	NADP binding
R	0051287	molecular_function	NAD binding

Functional Information from PROSITE/UniProt

site_id	PS00071
Number of Residues	8
Details	GAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL

Chain	Residue	Details
P	ALA147-LEU154
O	ALA147-LEU154

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PROSITE-ProRule","id":"PRU10009","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	32
Details	Binding site: {"evidences":[{"source":"PubMed","id":"17573533","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	96
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	16
Details	Site: {"description":"Activates thiol group during catalysis","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	8
Details	Active site: {"description":"Nucleophile"}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	32
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11846565","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12705826","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15236965","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17573533","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)