Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7Q54

Single Particle Cryo-EM structure of photosynthetic A4B4-glyceraldehyde 3-phosphate dehydrogenase from Spinacia oleracia.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
A	0006006	biological_process	glucose metabolic process
A	0009507	cellular_component	chloroplast
A	0016491	molecular_function	oxidoreductase activity
A	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
A	0019253	biological_process	reductive pentose-phosphate cycle
A	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
A	0050661	molecular_function	NADP binding
A	0051287	molecular_function	NAD binding
B	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
B	0006006	biological_process	glucose metabolic process
B	0009507	cellular_component	chloroplast
B	0016491	molecular_function	oxidoreductase activity
B	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
B	0019253	biological_process	reductive pentose-phosphate cycle
B	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
B	0048046	cellular_component	apoplast
B	0050661	molecular_function	NADP binding
B	0051287	molecular_function	NAD binding
C	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
C	0006006	biological_process	glucose metabolic process
C	0009507	cellular_component	chloroplast
C	0016491	molecular_function	oxidoreductase activity
C	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
C	0019253	biological_process	reductive pentose-phosphate cycle
C	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
C	0050661	molecular_function	NADP binding
C	0051287	molecular_function	NAD binding
D	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
D	0006006	biological_process	glucose metabolic process
D	0009507	cellular_component	chloroplast
D	0016491	molecular_function	oxidoreductase activity
D	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
D	0019253	biological_process	reductive pentose-phosphate cycle
D	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
D	0048046	cellular_component	apoplast
D	0050661	molecular_function	NADP binding
D	0051287	molecular_function	NAD binding
O	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
O	0006006	biological_process	glucose metabolic process
O	0009507	cellular_component	chloroplast
O	0016491	molecular_function	oxidoreductase activity
O	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
O	0019253	biological_process	reductive pentose-phosphate cycle
O	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
O	0050661	molecular_function	NADP binding
O	0051287	molecular_function	NAD binding
P	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
P	0006006	biological_process	glucose metabolic process
P	0009507	cellular_component	chloroplast
P	0016491	molecular_function	oxidoreductase activity
P	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
P	0019253	biological_process	reductive pentose-phosphate cycle
P	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
P	0048046	cellular_component	apoplast
P	0050661	molecular_function	NADP binding
P	0051287	molecular_function	NAD binding
Q	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
Q	0006006	biological_process	glucose metabolic process
Q	0009507	cellular_component	chloroplast
Q	0016491	molecular_function	oxidoreductase activity
Q	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
Q	0019253	biological_process	reductive pentose-phosphate cycle
Q	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
Q	0050661	molecular_function	NADP binding
Q	0051287	molecular_function	NAD binding
R	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
R	0006006	biological_process	glucose metabolic process
R	0009507	cellular_component	chloroplast
R	0016491	molecular_function	oxidoreductase activity
R	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
R	0019253	biological_process	reductive pentose-phosphate cycle
R	0047100	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity
R	0048046	cellular_component	apoplast
R	0050661	molecular_function	NADP binding
R	0051287	molecular_function	NAD binding

Functional Information from PROSITE/UniProt

site_id	PS00071
Number of Residues	8
Details	GAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL

Chain	Residue	Details
R	ALA147-LEU154
Q	ALA147-LEU154

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Nucleophile

Chain	Residue	Details
R	CYS149
P	CYS149
B	CYS149
D	CYS149

site_id	SWS_FT_FI2
Number of Residues	16
Details	BINDING: BINDING => ECO:0000269\|PubMed:11846565, ECO:0000269\|PubMed:12705826, ECO:0000269\|PubMed:15236965, ECO:0000269\|PubMed:17573533

Chain	Residue	Details
R	ARG10
B	ASP32
B	ARG77
B	ASN313
D	ARG10
D	ASP32
D	ARG77
D	ASN313
R	ASP32
R	ARG77
R	ASN313
P	ARG10
P	ASP32
P	ARG77
P	ASN313
B	ARG10

site_id	SWS_FT_FI3
Number of Residues	20
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
R	SER148
P	ARG231
B	SER148
B	THR179
B	ARG195
B	THR208
B	ARG231
D	SER148
D	THR179
D	ARG195
D	THR208
R	THR179
D	ARG231
R	ARG195
R	THR208
R	ARG231
P	SER148
P	THR179
P	ARG195
P	THR208

site_id	SWS_FT_FI4
Number of Residues	4
Details	SITE: Activates thiol group during catalysis => ECO:0000250

Chain	Residue	Details
R	HIS176
P	HIS176
B	HIS176
D	HIS176

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)