7PTH

C54S mutant of choline-sulfatase from E. meliloti CECT4857 bound to choline

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004423	molecular_function	iduronate-2-sulfatase activity
A	0005737	cellular_component	cytoplasm
A	0016787	molecular_function	hydrolase activity
A	0042425	biological_process	choline biosynthetic process
A	0046872	molecular_function	metal ion binding
A	0047753	molecular_function	choline-sulfatase activity
B	0004423	molecular_function	iduronate-2-sulfatase activity
B	0005737	cellular_component	cytoplasm
B	0016787	molecular_function	hydrolase activity
B	0042425	biological_process	choline biosynthetic process
B	0046872	molecular_function	metal ion binding
B	0047753	molecular_function	choline-sulfatase activity
C	0004423	molecular_function	iduronate-2-sulfatase activity
C	0005737	cellular_component	cytoplasm
C	0016787	molecular_function	hydrolase activity
C	0042425	biological_process	choline biosynthetic process
C	0046872	molecular_function	metal ion binding
C	0047753	molecular_function	choline-sulfatase activity
D	0004423	molecular_function	iduronate-2-sulfatase activity
D	0005737	cellular_component	cytoplasm
D	0016787	molecular_function	hydrolase activity
D	0042425	biological_process	choline biosynthetic process
D	0046872	molecular_function	metal ion binding
D	0047753	molecular_function	choline-sulfatase activity

Functional Information from PROSITE/UniProt

site_id	PS00149
Number of Residues	11
Details	SULFATASE_2 Sulfatases signature 2. GYyTalSGK.MH

Chain	Residue	Details
A	GLY94-HIS104

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site_id	PS00523
Number of Residues	13
Details	SULFATASE_1 Sulfatases signature 1. PLSAPARasFMAG

Chain	Residue	Details
A	PRO52-GLY64

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Nucleophile => ECO:0000250|UniProtKB:P15289

Chain	Residue	Details
A	SER54
B	SER54
C	SER54
D	SER54

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site_id	SWS_FT_FI2
Number of Residues	4
Details	ACT_SITE: ACT_SITE => ECO:0000250|UniProtKB:P15289

Chain	Residue	Details
A	HIS104
B	HIS104
C	HIS104
D	HIS104

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site_id	SWS_FT_FI3
Number of Residues	16
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
A	ASP14
A	GLN15
A	ASP296
A	HIS297
B	ASP14
B	GLN15
B	ASP296
B	HIS297
C	ASP14
C	GLN15
C	ASP296
C	HIS297
D	ASP14
D	GLN15
D	ASP296
D	HIS297

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site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: via 3-oxoalanine => ECO:0000250

Chain	Residue	Details
A	SER54
B	SER54
C	SER54
D	SER54

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site_id	SWS_FT_FI5
Number of Residues	4
Details	MOD_RES: 3-oxoalanine (Cys) => ECO:0000250|UniProtKB:P15289

Chain	Residue	Details
A	SER54
B	SER54
C	SER54
D	SER54

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