Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7P7L

Complex I from E. coli, DDM/LMNG-purified, with NADH and FMN, Open state

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003954	molecular_function	NADH dehydrogenase activity
A	0005886	cellular_component	plasma membrane
A	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
A	0016020	cellular_component	membrane
A	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
A	0022904	biological_process	respiratory electron transport chain
A	0030964	cellular_component	NADH dehydrogenase complex
A	0045271	cellular_component	respiratory chain complex I
A	0048038	molecular_function	quinone binding
A	0050136	molecular_function	NADH:ubiquinone reductase (non-electrogenic) activity
A	1902600	biological_process	proton transmembrane transport
B	0003954	molecular_function	NADH dehydrogenase activity
B	0005506	molecular_function	iron ion binding
B	0005886	cellular_component	plasma membrane
B	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
B	0009060	biological_process	aerobic respiration
B	0015990	biological_process	electron transport coupled proton transport
B	0016020	cellular_component	membrane
B	0022904	biological_process	respiratory electron transport chain
B	0030964	cellular_component	NADH dehydrogenase complex
B	0045271	cellular_component	respiratory chain complex I
B	0046872	molecular_function	metal ion binding
B	0048038	molecular_function	quinone binding
B	0050136	molecular_function	NADH:ubiquinone reductase (non-electrogenic) activity
B	0051536	molecular_function	iron-sulfur cluster binding
B	0051539	molecular_function	4 iron, 4 sulfur cluster binding
C	0003824	molecular_function	catalytic activity
C	0003954	molecular_function	NADH dehydrogenase activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005886	cellular_component	plasma membrane
C	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
C	0016020	cellular_component	membrane
C	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
C	0022904	biological_process	respiratory electron transport chain
C	0030964	cellular_component	NADH dehydrogenase complex
C	0045271	cellular_component	respiratory chain complex I
C	0048038	molecular_function	quinone binding
C	0050136	molecular_function	NADH:ubiquinone reductase (non-electrogenic) activity
C	0051287	molecular_function	NAD binding
C	1902600	biological_process	proton transmembrane transport
E	0003954	molecular_function	NADH dehydrogenase activity
E	0005515	molecular_function	protein binding
E	0005886	cellular_component	plasma membrane
E	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
E	0010181	molecular_function	FMN binding
E	0016020	cellular_component	membrane
E	0016491	molecular_function	oxidoreductase activity
E	0022904	biological_process	respiratory electron transport chain
E	0030964	cellular_component	NADH dehydrogenase complex
E	0045271	cellular_component	respiratory chain complex I
E	0046872	molecular_function	metal ion binding
E	0048038	molecular_function	quinone binding
E	0051537	molecular_function	2 iron, 2 sulfur cluster binding
E	1902600	biological_process	proton transmembrane transport
F	0003954	molecular_function	NADH dehydrogenase activity
F	0005886	cellular_component	plasma membrane
F	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
F	0009060	biological_process	aerobic respiration
F	0010181	molecular_function	FMN binding
F	0016020	cellular_component	membrane
F	0022904	biological_process	respiratory electron transport chain
F	0030964	cellular_component	NADH dehydrogenase complex
F	0045271	cellular_component	respiratory chain complex I
F	0045333	biological_process	cellular respiration
F	0046872	molecular_function	metal ion binding
F	0048038	molecular_function	quinone binding
F	0051287	molecular_function	NAD binding
F	0051539	molecular_function	4 iron, 4 sulfur cluster binding
F	1902600	biological_process	proton transmembrane transport
G	0003954	molecular_function	NADH dehydrogenase activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005886	cellular_component	plasma membrane
G	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
G	0009060	biological_process	aerobic respiration
G	0010181	molecular_function	FMN binding
G	0016020	cellular_component	membrane
G	0016491	molecular_function	oxidoreductase activity
G	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
G	0022904	biological_process	respiratory electron transport chain
G	0030964	cellular_component	NADH dehydrogenase complex
G	0042773	biological_process	ATP synthesis coupled electron transport
G	0043546	molecular_function	molybdopterin cofactor binding
G	0045271	cellular_component	respiratory chain complex I
G	0045333	biological_process	cellular respiration
G	0046872	molecular_function	metal ion binding
G	0048038	molecular_function	quinone binding
G	0051536	molecular_function	iron-sulfur cluster binding
G	0051537	molecular_function	2 iron, 2 sulfur cluster binding
G	0051539	molecular_function	4 iron, 4 sulfur cluster binding
G	1902600	biological_process	proton transmembrane transport
G	1990204	cellular_component	oxidoreductase complex
H	0003954	molecular_function	NADH dehydrogenase activity
H	0005886	cellular_component	plasma membrane
H	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
H	0009060	biological_process	aerobic respiration
H	0016020	cellular_component	membrane
H	0016655	molecular_function	oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
H	0022904	biological_process	respiratory electron transport chain
H	0030964	cellular_component	NADH dehydrogenase complex
H	0045271	cellular_component	respiratory chain complex I
H	0048038	molecular_function	quinone binding
H	1902600	biological_process	proton transmembrane transport
I	0003954	molecular_function	NADH dehydrogenase activity
I	0005506	molecular_function	iron ion binding
I	0005886	cellular_component	plasma membrane
I	0009060	biological_process	aerobic respiration
I	0016020	cellular_component	membrane
I	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
I	0022904	biological_process	respiratory electron transport chain
I	0030964	cellular_component	NADH dehydrogenase complex
I	0045271	cellular_component	respiratory chain complex I
I	0046872	molecular_function	metal ion binding
I	0048038	molecular_function	quinone binding
I	0050136	molecular_function	NADH:ubiquinone reductase (non-electrogenic) activity
I	0051539	molecular_function	4 iron, 4 sulfur cluster binding
J	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
K	0003954	molecular_function	NADH dehydrogenase activity
K	0005515	molecular_function	protein binding
K	0005886	cellular_component	plasma membrane
K	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
K	0016020	cellular_component	membrane
K	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
K	0022904	biological_process	respiratory electron transport chain
K	0030964	cellular_component	NADH dehydrogenase complex
K	0042773	biological_process	ATP synthesis coupled electron transport
K	0045271	cellular_component	respiratory chain complex I
K	0048038	molecular_function	quinone binding
K	0050136	molecular_function	NADH:ubiquinone reductase (non-electrogenic) activity
K	1902600	biological_process	proton transmembrane transport
L	0003954	molecular_function	NADH dehydrogenase activity
L	0005886	cellular_component	plasma membrane
L	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
L	0015990	biological_process	electron transport coupled proton transport
L	0016020	cellular_component	membrane
L	0022904	biological_process	respiratory electron transport chain
L	0030964	cellular_component	NADH dehydrogenase complex
L	0042773	biological_process	ATP synthesis coupled electron transport
L	0045271	cellular_component	respiratory chain complex I
L	0048038	molecular_function	quinone binding
M	0003954	molecular_function	NADH dehydrogenase activity
M	0005886	cellular_component	plasma membrane
M	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
M	0009060	biological_process	aerobic respiration
M	0015990	biological_process	electron transport coupled proton transport
M	0016020	cellular_component	membrane
M	0022904	biological_process	respiratory electron transport chain
M	0030964	cellular_component	NADH dehydrogenase complex
M	0042773	biological_process	ATP synthesis coupled electron transport
M	0045271	cellular_component	respiratory chain complex I
M	0048038	molecular_function	quinone binding
M	0048039	molecular_function	ubiquinone binding
N	0003954	molecular_function	NADH dehydrogenase activity
N	0005886	cellular_component	plasma membrane
N	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
N	0009060	biological_process	aerobic respiration
N	0015990	biological_process	electron transport coupled proton transport
N	0016020	cellular_component	membrane
N	0022904	biological_process	respiratory electron transport chain
N	0030964	cellular_component	NADH dehydrogenase complex
N	0042773	biological_process	ATP synthesis coupled electron transport
N	0045271	cellular_component	respiratory chain complex I
N	0048038	molecular_function	quinone binding
N	0050136	molecular_function	NADH:ubiquinone reductase (non-electrogenic) activity

Functional Information from PROSITE/UniProt

site_id	PS00198
Number of Residues	12
Details	4FE4S_FER_1 4Fe-4S ferredoxin-type iron-sulfur binding region signature. CvACNlCAvACP

Chain	Residue	Details
I	CYS60-PRO71
I	CYS99-PRO110

site_id	PS00535
Number of Residues	12
Details	COMPLEX1_49K Respiratory chain NADH dehydrogenase 49 Kd subunit signature. HHRGaEKMgErQ

Chain	Residue	Details
C	HIS252-GLN263

site_id	PS00542
Number of Residues	22
Details	COMPLEX1_30K Respiratory chain NADH dehydrogenase 30 Kd subunit signature. EREtwDLFgitfdgHpnlRrIM

Chain	Residue	Details
C	GLU138-MET159

site_id	PS00641
Number of Residues	18
Details	COMPLEX1_75K_1 Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 1. PyfCWhpalgsvGaCRqC

Chain	Residue	Details
G	PRO33-CYS50

site_id	PS00642
Number of Residues	13
Details	COMPLEX1_75K_2 Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2. CPvCEeGGnChLQ

Chain	Residue	Details
G	CYS105-GLN117

site_id	PS00643
Number of Residues	11
Details	COMPLEX1_75K_3 Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. RCIaCyRCVrY

Chain	Residue	Details
G	ARG152-TYR162

site_id	PS00644
Number of Residues	16
Details	COMPLEX1_51K_1 Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 1. GAGRYICGEETALINS

Chain	Residue	Details
F	GLY174-SER189

site_id	PS00645
Number of Residues	12
Details	COMPLEX1_51K_2 Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 2. ESCGwCtPCRdG

Chain	Residue	Details
F	GLU349-GLY360

site_id	PS00667
Number of Residues	16
Details	COMPLEX1_ND1_1 Respiratory-chain NADH dehydrogenase subunit 1 signature 1. GSLQlVaDMIKMFfKE

Chain	Residue	Details
H	GLY56-GLU71

site_id	PS00668
Number of Residues	14
Details	COMPLEX1_ND1_2 Respiratory-chain NADH dehydrogenase subunit 1 signature 2. PFDQPEAEq.ELadG

Chain	Residue	Details
H	PRO211-GLY224

site_id	PS01099
Number of Residues	19
Details	COMPLEX1_24K Respiratory-chain NADH dehydrogenase 24 Kd subunit signature. DgrFTllptcCLGnCdkGP

Chain	Residue	Details
E	ASP123-PRO141

site_id	PS01150
Number of Residues	17
Details	COMPLEX1_20K Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. GvDKFIPVDVYiPgCPP

Chain	Residue	Details
B	GLY144-PRO160

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	86
Details	TOPO_DOM: Periplasmic => ECO:0000255

Chain	Residue	Details
N	MET1-ASN7
M	GLY340-GLY360
M	TRP383-ASN403
M	PHE404-ILE424
M	THR426-GLY446
M	LEU465-ILE485
N	GLY56-ASP70
N	ALA126
N	GLN180-LEU202
N	ARG256-ARG270
N	THR318-ALA325
N	GLY394-LEU407
N	SER476-MET485
M	LEU314-SER334

site_id	SWS_FT_FI2
Number of Residues	282
Details	TRANSMEM: Helical => ECO:0000255\|HAMAP-Rule:MF_00445

Chain	Residue	Details
N	LEU8-ILE28
N	LEU297-GLN317
N	VAL326-LEU346
N	ALA373-ILE393
N	TRP408-VAL430
N	ILE455-ILE475
N	PHE35-VAL55
N	GLY71-ALA91
N	PHE105-LEU125
N	SER127-PHE147
N	TYR159-ALA179
N	LEU203-PHE223
N	PRO235-MET255
N	VAL271-GLN291

site_id	SWS_FT_FI3
Number of Residues	89
Details	TOPO_DOM: Cytoplasmic => ECO:0000255

Chain	Residue	Details
N	ALA29-HIS34
N	TYR92-GLU104
N	ARG148-LYS158
N	HIS224-ALA234
N	THR292-ARG296
N	MET347-LEU372
N	SER431-GLY454

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)