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7P6K

Structure of homomeric LRRC8A Volume-Regulated Anion Channel in complex with synthetic nanobody Sb5

Functional Information from GO Data
ChainGOidnamespacecontents
A0001678biological_processintracellular glucose homeostasis
A0002329biological_processpre-B cell differentiation
A0005225molecular_functionvolume-sensitive anion channel activity
A0005253molecular_functionmonoatomic anion channel activity
A0005764cellular_componentlysosome
A0005765cellular_componentlysosomal membrane
A0005886cellular_componentplasma membrane
A0006820biological_processmonoatomic anion transport
A0006884biological_processcell volume homeostasis
A0006970biological_processresponse to osmotic stress
A0007283biological_processspermatogenesis
A0009986cellular_componentcell surface
A0015734biological_processtaurine transmembrane transport
A0015810biological_processaspartate transmembrane transport
A0016020cellular_componentmembrane
A0030154biological_processcell differentiation
A0032024biological_processpositive regulation of insulin secretion
A0034214biological_processprotein hexamerization
A0034220biological_processmonoatomic ion transmembrane transport
A0034702cellular_componentmonoatomic ion channel complex
A0042802molecular_functionidentical protein binding
A0045663biological_processpositive regulation of myoblast differentiation
A0055085biological_processtransmembrane transport
A0098656biological_processmonoatomic anion transmembrane transport
A0140360molecular_functioncyclic-GMP-AMP transmembrane transporter activity
A0140361biological_processcyclic-GMP-AMP transmembrane import across plasma membrane
A1902476biological_processchloride transmembrane transport
B0001678biological_processintracellular glucose homeostasis
B0002329biological_processpre-B cell differentiation
B0005225molecular_functionvolume-sensitive anion channel activity
B0005253molecular_functionmonoatomic anion channel activity
B0005764cellular_componentlysosome
B0005765cellular_componentlysosomal membrane
B0005886cellular_componentplasma membrane
B0006820biological_processmonoatomic anion transport
B0006884biological_processcell volume homeostasis
B0006970biological_processresponse to osmotic stress
B0007283biological_processspermatogenesis
B0009986cellular_componentcell surface
B0015734biological_processtaurine transmembrane transport
B0015810biological_processaspartate transmembrane transport
B0016020cellular_componentmembrane
B0030154biological_processcell differentiation
B0032024biological_processpositive regulation of insulin secretion
B0034214biological_processprotein hexamerization
B0034220biological_processmonoatomic ion transmembrane transport
B0034702cellular_componentmonoatomic ion channel complex
B0042802molecular_functionidentical protein binding
B0045663biological_processpositive regulation of myoblast differentiation
B0055085biological_processtransmembrane transport
B0098656biological_processmonoatomic anion transmembrane transport
B0140360molecular_functioncyclic-GMP-AMP transmembrane transporter activity
B0140361biological_processcyclic-GMP-AMP transmembrane import across plasma membrane
B1902476biological_processchloride transmembrane transport
C0001678biological_processintracellular glucose homeostasis
C0002329biological_processpre-B cell differentiation
C0005225molecular_functionvolume-sensitive anion channel activity
C0005253molecular_functionmonoatomic anion channel activity
C0005764cellular_componentlysosome
C0005765cellular_componentlysosomal membrane
C0005886cellular_componentplasma membrane
C0006820biological_processmonoatomic anion transport
C0006884biological_processcell volume homeostasis
C0006970biological_processresponse to osmotic stress
C0007283biological_processspermatogenesis
C0009986cellular_componentcell surface
C0015734biological_processtaurine transmembrane transport
C0015810biological_processaspartate transmembrane transport
C0016020cellular_componentmembrane
C0030154biological_processcell differentiation
C0032024biological_processpositive regulation of insulin secretion
C0034214biological_processprotein hexamerization
C0034220biological_processmonoatomic ion transmembrane transport
C0034702cellular_componentmonoatomic ion channel complex
C0042802molecular_functionidentical protein binding
C0045663biological_processpositive regulation of myoblast differentiation
C0055085biological_processtransmembrane transport
C0098656biological_processmonoatomic anion transmembrane transport
C0140360molecular_functioncyclic-GMP-AMP transmembrane transporter activity
C0140361biological_processcyclic-GMP-AMP transmembrane import across plasma membrane
C1902476biological_processchloride transmembrane transport
D0001678biological_processintracellular glucose homeostasis
D0002329biological_processpre-B cell differentiation
D0005225molecular_functionvolume-sensitive anion channel activity
D0005253molecular_functionmonoatomic anion channel activity
D0005764cellular_componentlysosome
D0005765cellular_componentlysosomal membrane
D0005886cellular_componentplasma membrane
D0006820biological_processmonoatomic anion transport
D0006884biological_processcell volume homeostasis
D0006970biological_processresponse to osmotic stress
D0007283biological_processspermatogenesis
D0009986cellular_componentcell surface
D0015734biological_processtaurine transmembrane transport
D0015810biological_processaspartate transmembrane transport
D0016020cellular_componentmembrane
D0030154biological_processcell differentiation
D0032024biological_processpositive regulation of insulin secretion
D0034214biological_processprotein hexamerization
D0034220biological_processmonoatomic ion transmembrane transport
D0034702cellular_componentmonoatomic ion channel complex
D0042802molecular_functionidentical protein binding
D0045663biological_processpositive regulation of myoblast differentiation
D0055085biological_processtransmembrane transport
D0098656biological_processmonoatomic anion transmembrane transport
D0140360molecular_functioncyclic-GMP-AMP transmembrane transporter activity
D0140361biological_processcyclic-GMP-AMP transmembrane import across plasma membrane
D1902476biological_processchloride transmembrane transport
E0001678biological_processintracellular glucose homeostasis
E0002329biological_processpre-B cell differentiation
E0005225molecular_functionvolume-sensitive anion channel activity
E0005253molecular_functionmonoatomic anion channel activity
E0005764cellular_componentlysosome
E0005765cellular_componentlysosomal membrane
E0005886cellular_componentplasma membrane
E0006820biological_processmonoatomic anion transport
E0006884biological_processcell volume homeostasis
E0006970biological_processresponse to osmotic stress
E0007283biological_processspermatogenesis
E0009986cellular_componentcell surface
E0015734biological_processtaurine transmembrane transport
E0015810biological_processaspartate transmembrane transport
E0016020cellular_componentmembrane
E0030154biological_processcell differentiation
E0032024biological_processpositive regulation of insulin secretion
E0034214biological_processprotein hexamerization
E0034220biological_processmonoatomic ion transmembrane transport
E0034702cellular_componentmonoatomic ion channel complex
E0042802molecular_functionidentical protein binding
E0045663biological_processpositive regulation of myoblast differentiation
E0055085biological_processtransmembrane transport
E0098656biological_processmonoatomic anion transmembrane transport
E0140360molecular_functioncyclic-GMP-AMP transmembrane transporter activity
E0140361biological_processcyclic-GMP-AMP transmembrane import across plasma membrane
E1902476biological_processchloride transmembrane transport
F0001678biological_processintracellular glucose homeostasis
F0002329biological_processpre-B cell differentiation
F0005225molecular_functionvolume-sensitive anion channel activity
F0005253molecular_functionmonoatomic anion channel activity
F0005764cellular_componentlysosome
F0005765cellular_componentlysosomal membrane
F0005886cellular_componentplasma membrane
F0006820biological_processmonoatomic anion transport
F0006884biological_processcell volume homeostasis
F0006970biological_processresponse to osmotic stress
F0007283biological_processspermatogenesis
F0009986cellular_componentcell surface
F0015734biological_processtaurine transmembrane transport
F0015810biological_processaspartate transmembrane transport
F0016020cellular_componentmembrane
F0030154biological_processcell differentiation
F0032024biological_processpositive regulation of insulin secretion
F0034214biological_processprotein hexamerization
F0034220biological_processmonoatomic ion transmembrane transport
F0034702cellular_componentmonoatomic ion channel complex
F0042802molecular_functionidentical protein binding
F0045663biological_processpositive regulation of myoblast differentiation
F0055085biological_processtransmembrane transport
F0098656biological_processmonoatomic anion transmembrane transport
F0140360molecular_functioncyclic-GMP-AMP transmembrane transporter activity
F0140361biological_processcyclic-GMP-AMP transmembrane import across plasma membrane
F1902476biological_processchloride transmembrane transport
Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues3660
DetailsTOPO_DOM: Cytoplasmic => ECO:0000305|PubMed:29769723
ChainResidueDetails
AMET1-PRO22
DMET1-PRO22
DTRP143-TYR264
DTRP342-ALA810
EMET1-PRO22
ETRP143-TYR264
ETRP342-ALA810
FMET1-PRO22
FTRP143-TYR264
FTRP342-ALA810
ATRP143-TYR264
ATRP342-ALA810
BMET1-PRO22
BTRP143-TYR264
BTRP342-ALA810
CMET1-PRO22
CTRP143-TYR264
CTRP342-ALA810

site_idSWS_FT_FI2
Number of Residues522
DetailsTRANSMEM: Helical => ECO:0000305|PubMed:30775971, ECO:0007744|PDB:6NZW, ECO:0007744|PDB:6NZZ, ECO:0007744|PDB:6O00
ChainResidueDetails
ATRP23-VAL47
CTYR124-PHE142
CMET265-VAL286
CLEU317-TRP341
DTRP23-VAL47
DTYR124-PHE142
DMET265-VAL286
DLEU317-TRP341
ETRP23-VAL47
ETYR124-PHE142
EMET265-VAL286
ATYR124-PHE142
ELEU317-TRP341
FTRP23-VAL47
FTYR124-PHE142
FMET265-VAL286
FLEU317-TRP341
AMET265-VAL286
ALEU317-TRP341
BTRP23-VAL47
BTYR124-PHE142
BMET265-VAL286
BLEU317-TRP341
CTRP23-VAL47

site_idSWS_FT_FI3
Number of Residues624
DetailsTOPO_DOM: Extracellular => ECO:0000305|PubMed:29769723
ChainResidueDetails
ATHR48-LYS123
EHIS287-THR316
FTHR48-LYS123
FHIS287-THR316
AHIS287-THR316
BTHR48-LYS123
BHIS287-THR316
CTHR48-LYS123
CHIS287-THR316
DTHR48-LYS123
DHIS287-THR316
ETHR48-LYS123

site_idSWS_FT_FI4
Number of Residues6
DetailsSITE: Required for anion selectivity => ECO:0000250|UniProtKB:Q8IWT6
ChainResidueDetails
AARG103
BARG103
CARG103
DARG103
EARG103
FARG103

site_idSWS_FT_FI5
Number of Residues6
DetailsMOD_RES: N-acetylmethionine => ECO:0000250|UniProtKB:Q8IWT6
ChainResidueDetails
AMET1
BMET1
CMET1
DMET1
EMET1
FMET1

site_idSWS_FT_FI6
Number of Residues6
DetailsMOD_RES: Phosphothreonine => ECO:0007744|PubMed:21183079
ChainResidueDetails
ATHR200
BTHR200
CTHR200
DTHR200
ETHR200
FTHR200

site_idSWS_FT_FI7
Number of Residues6
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:21183079
ChainResidueDetails
ASER202
BSER202
CSER202
DSER202
ESER202
FSER202

site_idSWS_FT_FI8
Number of Residues6
DetailsMOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:Q8IWT6
ChainResidueDetails
ATHR215
BTHR215
CTHR215
DTHR215
ETHR215
FTHR215

site_idSWS_FT_FI9
Number of Residues6
DetailsMOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q8IWT6
ChainResidueDetails
ASER217
BSER217
CSER217
DSER217
ESER217
FSER217

site_idSWS_FT_FI10
Number of Residues12
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255
ChainResidueDetails
AASN66
EASN83
FASN66
FASN83
AASN83
BASN66
BASN83
CASN66
CASN83
DASN66
DASN83
EASN66

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PDB entries from 2024-10-30

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