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7OR1

Cryo-EM structure of the human TRPA1 ion channel in complex with the antagonist 3-60, conformation 1

Functional Information from GO Data
ChainGOidnamespacecontents
A0005216molecular_functionmonoatomic ion channel activity
A0005262molecular_functioncalcium channel activity
A0005886cellular_componentplasma membrane
A0006811biological_processmonoatomic ion transport
A0006816biological_processcalcium ion transport
A0006874biological_processintracellular calcium ion homeostasis
A0007166biological_processcell surface receptor signaling pathway
A0009409biological_processresponse to cold
A0009410biological_processresponse to xenobiotic stimulus
A0014070biological_processresponse to organic cyclic compound
A0015267molecular_functionchannel activity
A0015278molecular_functionintracellularly gated calcium channel activity
A0016020cellular_componentmembrane
A0019233biological_processsensory perception of pain
A0019722biological_processcalcium-mediated signaling
A0032421cellular_componentstereocilium bundle
A0042542biological_processresponse to hydrogen peroxide
A0042802molecular_functionidentical protein binding
A0048265biological_processresponse to pain
A0050955biological_processthermoception
A0050966biological_processdetection of mechanical stimulus involved in sensory perception of pain
A0050968biological_processdetection of chemical stimulus involved in sensory perception of pain
A0050974biological_processdetection of mechanical stimulus involved in sensory perception
A0051289biological_processprotein homotetramerization
A0055085biological_processtransmembrane transport
A0070301biological_processcellular response to hydrogen peroxide
A0070588biological_processcalcium ion transmembrane transport
A0097604molecular_functiontemperature-gated cation channel activity
B0005216molecular_functionmonoatomic ion channel activity
B0005262molecular_functioncalcium channel activity
B0005886cellular_componentplasma membrane
B0006811biological_processmonoatomic ion transport
B0006816biological_processcalcium ion transport
B0006874biological_processintracellular calcium ion homeostasis
B0007166biological_processcell surface receptor signaling pathway
B0009409biological_processresponse to cold
B0009410biological_processresponse to xenobiotic stimulus
B0014070biological_processresponse to organic cyclic compound
B0015267molecular_functionchannel activity
B0015278molecular_functionintracellularly gated calcium channel activity
B0016020cellular_componentmembrane
B0019233biological_processsensory perception of pain
B0019722biological_processcalcium-mediated signaling
B0032421cellular_componentstereocilium bundle
B0042542biological_processresponse to hydrogen peroxide
B0042802molecular_functionidentical protein binding
B0048265biological_processresponse to pain
B0050955biological_processthermoception
B0050966biological_processdetection of mechanical stimulus involved in sensory perception of pain
B0050968biological_processdetection of chemical stimulus involved in sensory perception of pain
B0050974biological_processdetection of mechanical stimulus involved in sensory perception
B0051289biological_processprotein homotetramerization
B0055085biological_processtransmembrane transport
B0070301biological_processcellular response to hydrogen peroxide
B0070588biological_processcalcium ion transmembrane transport
B0097604molecular_functiontemperature-gated cation channel activity
C0005216molecular_functionmonoatomic ion channel activity
C0005262molecular_functioncalcium channel activity
C0005886cellular_componentplasma membrane
C0006811biological_processmonoatomic ion transport
C0006816biological_processcalcium ion transport
C0006874biological_processintracellular calcium ion homeostasis
C0007166biological_processcell surface receptor signaling pathway
C0009409biological_processresponse to cold
C0009410biological_processresponse to xenobiotic stimulus
C0014070biological_processresponse to organic cyclic compound
C0015267molecular_functionchannel activity
C0015278molecular_functionintracellularly gated calcium channel activity
C0016020cellular_componentmembrane
C0019233biological_processsensory perception of pain
C0019722biological_processcalcium-mediated signaling
C0032421cellular_componentstereocilium bundle
C0042542biological_processresponse to hydrogen peroxide
C0042802molecular_functionidentical protein binding
C0048265biological_processresponse to pain
C0050955biological_processthermoception
C0050966biological_processdetection of mechanical stimulus involved in sensory perception of pain
C0050968biological_processdetection of chemical stimulus involved in sensory perception of pain
C0050974biological_processdetection of mechanical stimulus involved in sensory perception
C0051289biological_processprotein homotetramerization
C0055085biological_processtransmembrane transport
C0070301biological_processcellular response to hydrogen peroxide
C0070588biological_processcalcium ion transmembrane transport
C0097604molecular_functiontemperature-gated cation channel activity
D0005216molecular_functionmonoatomic ion channel activity
D0005262molecular_functioncalcium channel activity
D0005886cellular_componentplasma membrane
D0006811biological_processmonoatomic ion transport
D0006816biological_processcalcium ion transport
D0006874biological_processintracellular calcium ion homeostasis
D0007166biological_processcell surface receptor signaling pathway
D0009409biological_processresponse to cold
D0009410biological_processresponse to xenobiotic stimulus
D0014070biological_processresponse to organic cyclic compound
D0015267molecular_functionchannel activity
D0015278molecular_functionintracellularly gated calcium channel activity
D0016020cellular_componentmembrane
D0019233biological_processsensory perception of pain
D0019722biological_processcalcium-mediated signaling
D0032421cellular_componentstereocilium bundle
D0042542biological_processresponse to hydrogen peroxide
D0042802molecular_functionidentical protein binding
D0048265biological_processresponse to pain
D0050955biological_processthermoception
D0050966biological_processdetection of mechanical stimulus involved in sensory perception of pain
D0050968biological_processdetection of chemical stimulus involved in sensory perception of pain
D0050974biological_processdetection of mechanical stimulus involved in sensory perception
D0051289biological_processprotein homotetramerization
D0055085biological_processtransmembrane transport
D0070301biological_processcellular response to hydrogen peroxide
D0070588biological_processcalcium ion transmembrane transport
D0097604molecular_functiontemperature-gated cation channel activity
Functional Information from PROSITE/UniProt
site_idPS00018
Number of Residues13
DetailsEF_HAND_1 EF-hand calcium-binding domain. DISDTRLLNegDL
ChainResidueDetails
AASP468-LEU480

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues3560
DetailsTOPO_DOM: Cytoplasmic => ECO:0000305
ChainResidueDetails
AMET1-ALA718
CGLN794-ASN798
CGLN851-MET862
CVAL961-PRO1119
DMET1-ALA718
DGLN794-ASN798
DGLN851-MET862
DVAL961-PRO1119
AGLN794-ASN798
AGLN851-MET862
AVAL961-PRO1119
BMET1-ALA718
BGLN794-ASN798
BGLN851-MET862
BVAL961-PRO1119
CMET1-ALA718

site_idSWS_FT_FI2
Number of Residues80
DetailsTRANSMEM: Helical; Name=1 => ECO:0000269|PubMed:31866091, ECO:0007744|PDB:6PQO
ChainResidueDetails
AHIS719-ASN739
BHIS719-ASN739
CHIS719-ASN739
DHIS719-ASN739

site_idSWS_FT_FI3
Number of Residues200
DetailsTOPO_DOM: Extracellular => ECO:0000305
ChainResidueDetails
AILE740-SER767
CVAL824-HIS829
CASN893-PRO901
CLEU923-HIS933
DILE740-SER767
DVAL824-HIS829
DASN893-PRO901
DLEU923-HIS933
AVAL824-HIS829
AASN893-PRO901
ALEU923-HIS933
BILE740-SER767
BVAL824-HIS829
BASN893-PRO901
BLEU923-HIS933
CILE740-SER767

site_idSWS_FT_FI4
Number of Residues100
DetailsTRANSMEM: Helical; Name=2 => ECO:0000269|PubMed:31866091, ECO:0007744|PDB:6PQO
ChainResidueDetails
ATYR768-PHE793
BTYR768-PHE793
CTYR768-PHE793
DTYR768-PHE793

site_idSWS_FT_FI5
Number of Residues96
DetailsTRANSMEM: Helical; Name=3 => ECO:0000269|PubMed:31866091, ECO:0007744|PDB:6PQO
ChainResidueDetails
ATYR799-PHE823
BTYR799-PHE823
CTYR799-PHE823
DTYR799-PHE823

site_idSWS_FT_FI6
Number of Residues80
DetailsTRANSMEM: Helical; Name=4 => ECO:0000269|PubMed:31866091, ECO:0007744|PDB:6PQO
ChainResidueDetails
ALEU830-LEU850
BLEU830-LEU850
CLEU830-LEU850
DLEU830-LEU850

site_idSWS_FT_FI7
Number of Residues116
DetailsTRANSMEM: Helical; Name=5 => ECO:0000269|PubMed:31866091, ECO:0007744|PDB:6PQO
ChainResidueDetails
ALEU863-LEU892
BLEU863-LEU892
CLEU863-LEU892
DLEU863-LEU892

site_idSWS_FT_FI8
Number of Residues80
DetailsINTRAMEM: Pore-forming => ECO:0000269|PubMed:31866091, ECO:0007744|PDB:6PQO
ChainResidueDetails
ALEU902-PHE922
BLEU902-PHE922
CLEU902-PHE922
DLEU902-PHE922

site_idSWS_FT_FI9
Number of Residues104
DetailsTRANSMEM: Helical; Name=6 => ECO:0000269|PubMed:31866091, ECO:0007744|PDB:6PQO
ChainResidueDetails
APRO934-ALA960
BPRO934-ALA960
CPRO934-ALA960
DPRO934-ALA960

site_idSWS_FT_FI10
Number of Residues8
DetailsBINDING: covalent => ECO:0000250|UniProtKB:Q8BLA8
ChainResidueDetails
ACYS414
ACYS421
BCYS414
BCYS421
CCYS414
CCYS421
DCYS414
DCYS421

site_idSWS_FT_FI11
Number of Residues4
DetailsBINDING: covalent; Cys highly reactive => ECO:0000250|UniProtKB:Q8BLA8, ECO:0000305|PubMed:17164327, ECO:0000305|PubMed:27241698
ChainResidueDetails
ACYS621
BCYS621
CCYS621
DCYS621

site_idSWS_FT_FI12
Number of Residues8
DetailsBINDING: covalent => ECO:0000305|PubMed:17164327
ChainResidueDetails
ACYS641
ALYS710
BCYS641
BLYS710
CCYS641
CLYS710
DCYS641
DLYS710

site_idSWS_FT_FI13
Number of Residues4
DetailsBINDING: covalent => ECO:0000305|PubMed:17164327, ECO:0000305|PubMed:27241698
ChainResidueDetails
ACYS665
BCYS665
CCYS665
DCYS665

site_idSWS_FT_FI14
Number of Residues16
DetailsBINDING: BINDING => ECO:0000269|PubMed:32641835, ECO:0007744|PDB:6V9V
ChainResidueDetails
AGLU788
CGLN791
CASN805
CGLU808
DGLU788
DGLN791
DASN805
DGLU808
AGLN791
AASN805
AGLU808
BGLU788
BGLN791
BASN805
BGLU808
CGLU788

site_idSWS_FT_FI15
Number of Residues4
DetailsBINDING: BINDING => ECO:0000305|PubMed:25855297
ChainResidueDetails
ALYS1046
BLYS1046
CLYS1046
DLYS1046

site_idSWS_FT_FI16
Number of Residues4
DetailsSITE: Required for C-621 reactivity => ECO:0000269|PubMed:27241698
ChainResidueDetails
ALYS620
BLYS620
CLYS620
DLYS620

site_idSWS_FT_FI17
Number of Residues4
DetailsSITE: Essential for electrophile activation. Sensor for electrophilic agents => ECO:0000269|PubMed:31866091, ECO:0000269|PubMed:32641835
ChainResidueDetails
ACYS621
BCYS621
CCYS621
DCYS621

site_idSWS_FT_FI18
Number of Residues4
DetailsSITE: Key residue for activation by the scorpion wasabi receptor toxin => ECO:0000269|PubMed:31447178
ChainResidueDetails
APRO622
BPRO622
CPRO622
DPRO622

site_idSWS_FT_FI19
Number of Residues8
DetailsSITE: Important residue for activation by the scorpion wasabi receptor toxin => ECO:0000269|PubMed:31447178
ChainResidueDetails
AMET634
ATHR646
BMET634
BTHR646
CMET634
CTHR646
DMET634
DTHR646

site_idSWS_FT_FI20
Number of Residues4
DetailsSITE: Important for electrophile activation => ECO:0000269|PubMed:31866091, ECO:0000269|PubMed:32641835
ChainResidueDetails
ACYS665
BCYS665
CCYS665
DCYS665

site_idSWS_FT_FI21
Number of Residues4
DetailsSITE: Crucial for calcium permeation => ECO:0000250|UniProtKB:Q6RI86
ChainResidueDetails
AASP915
BASP915
CASP915
DASP915

site_idSWS_FT_FI22
Number of Residues4
DetailsMOD_RES: 4-hydroxyproline; by EGLN1; transient; in normoxia and hyperoxia => ECO:0000269|PubMed:21873995
ChainResidueDetails
APRO394
BPRO394
CPRO394
DPRO394

site_idSWS_FT_FI23
Number of Residues8
DetailsMOD_RES: Cysteine sulfenic acid (-SOH); transient; in hyperoxia => ECO:0000305|PubMed:21873995
ChainResidueDetails
ACYS633
ACYS856
BCYS633
BCYS856
CCYS633
CCYS856
DCYS633
DCYS856

site_idSWS_FT_FI24
Number of Residues8
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255
ChainResidueDetails
AASN747
AASN753
BASN747
BASN753
CASN747
CASN753
DASN747
DASN753

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PDB entries from 2024-10-30

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