Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7NTM

Cryo-EM structure of S.cerevisiae native alcohol dehydrogenase 1 (ADH1) in its tetrameric apo state

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000947	biological_process	amino acid catabolic process to alcohol via Ehrlich pathway
A	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
A	0004552	molecular_function	octanol dehydrogenase (NAD+) activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005886	cellular_component	plasma membrane
A	0006116	biological_process	NADH oxidation
A	0008270	molecular_function	zinc ion binding
A	0016491	molecular_function	oxidoreductase activity
A	0019170	molecular_function	methylglyoxal reductase (NADH) activity
A	0019655	biological_process	glycolytic fermentation to ethanol
A	0042802	molecular_function	identical protein binding
A	0046872	molecular_function	metal ion binding
A	0047655	molecular_function	allyl-alcohol dehydrogenase activity
A	1904408	molecular_function	melatonin binding
A	1990362	molecular_function	butanol dehydrogenase (NAD+) activity
B	0000947	biological_process	amino acid catabolic process to alcohol via Ehrlich pathway
B	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
B	0004552	molecular_function	octanol dehydrogenase (NAD+) activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005886	cellular_component	plasma membrane
B	0006116	biological_process	NADH oxidation
B	0008270	molecular_function	zinc ion binding
B	0016491	molecular_function	oxidoreductase activity
B	0019170	molecular_function	methylglyoxal reductase (NADH) activity
B	0019655	biological_process	glycolytic fermentation to ethanol
B	0042802	molecular_function	identical protein binding
B	0046872	molecular_function	metal ion binding
B	0047655	molecular_function	allyl-alcohol dehydrogenase activity
B	1904408	molecular_function	melatonin binding
B	1990362	molecular_function	butanol dehydrogenase (NAD+) activity
C	0000947	biological_process	amino acid catabolic process to alcohol via Ehrlich pathway
C	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
C	0004552	molecular_function	octanol dehydrogenase (NAD+) activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005886	cellular_component	plasma membrane
C	0006116	biological_process	NADH oxidation
C	0008270	molecular_function	zinc ion binding
C	0016491	molecular_function	oxidoreductase activity
C	0019170	molecular_function	methylglyoxal reductase (NADH) activity
C	0019655	biological_process	glycolytic fermentation to ethanol
C	0042802	molecular_function	identical protein binding
C	0046872	molecular_function	metal ion binding
C	0047655	molecular_function	allyl-alcohol dehydrogenase activity
C	1904408	molecular_function	melatonin binding
C	1990362	molecular_function	butanol dehydrogenase (NAD+) activity
D	0000947	biological_process	amino acid catabolic process to alcohol via Ehrlich pathway
D	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
D	0004552	molecular_function	octanol dehydrogenase (NAD+) activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005886	cellular_component	plasma membrane
D	0006116	biological_process	NADH oxidation
D	0008270	molecular_function	zinc ion binding
D	0016491	molecular_function	oxidoreductase activity
D	0019170	molecular_function	methylglyoxal reductase (NADH) activity
D	0019655	biological_process	glycolytic fermentation to ethanol
D	0042802	molecular_function	identical protein binding
D	0046872	molecular_function	metal ion binding
D	0047655	molecular_function	allyl-alcohol dehydrogenase activity
D	1904408	molecular_function	melatonin binding
D	1990362	molecular_function	butanol dehydrogenase (NAD+) activity

Functional Information from PROSITE/UniProt

site_id	PS00059
Number of Residues	15
Details	ADH_ZINC Zinc-containing alcohol dehydrogenases signature. GHEgAGVvvgmGenV

Chain	Residue	Details
A	GLY65-VAL79

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	28
Details	BINDING: BINDING => ECO:0000269\|PubMed:25157460, ECO:0000269\|PubMed:26743849, ECO:0007744\|PDB:4W6Z, ECO:0007744\|PDB:5ENV

Chain	Residue	Details
A	CYS43
B	CYS97
B	CYS100
B	CYS103
B	CYS111
B	CYS153
D	CYS43
D	HIS66
D	CYS97
D	CYS100
D	CYS103
A	HIS66
D	CYS111
D	CYS153
C	CYS43
C	HIS66
C	CYS97
C	CYS100
C	CYS103
C	CYS111
C	CYS153
A	CYS97
A	CYS100
A	CYS103
A	CYS111
A	CYS153
B	CYS43
B	HIS66

site_id	SWS_FT_FI2
Number of Residues	56
Details	BINDING: BINDING => ECO:0000269\|PubMed:26743849, ECO:0007744\|PDB:5ENV

Chain	Residue	Details
A	HIS44
A	VAL268
A	MET270
A	SER293
A	VAL295
A	ARG340
B	HIS44
B	THR45
B	HIS48
B	GLY180
B	GLY181
A	THR45
B	LEU182
B	ASP201
B	LYS206
B	PHE221
B	VAL268
B	MET270
B	SER293
B	VAL295
B	ARG340
D	HIS44
A	HIS48
D	THR45
D	HIS48
D	GLY180
D	GLY181
D	LEU182
D	ASP201
D	LYS206
D	PHE221
D	VAL268
D	MET270
A	GLY180
D	SER293
D	VAL295
D	ARG340
C	HIS44
C	THR45
C	HIS48
C	GLY180
C	GLY181
C	LEU182
C	ASP201
A	GLY181
C	LYS206
C	PHE221
C	VAL268
C	MET270
C	SER293
C	VAL295
C	ARG340
A	LEU182
A	ASP201
A	LYS206
A	PHE221

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: in the open conformation => ECO:0000269\|PubMed:25157460, ECO:0000269\|PubMed:26743849, ECO:0007744\|PDB:4W6Z, ECO:0007744\|PDB:5ENV

Chain	Residue	Details
A	GLU67
B	GLU67
D	GLU67
C	GLU67

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: N-acetylserine => ECO:0000269\|PubMed:320000

Chain	Residue	Details
A	SER1
B	SER1
D	SER1
C	SER1

site_id	SWS_FT_FI5
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:17287358

Chain	Residue	Details
A	SER212
B	SER212
D	SER212
C	SER212

site_id	SWS_FT_FI6
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:17287358

Chain	Residue	Details
A	THR222
B	THR222
D	THR222
C	THR222

site_id	SWS_FT_FI7
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:19779198

Chain	Residue	Details
A	SER278
B	SER278
D	SER278
C	SER278

site_id	SWS_FT_FI8
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:15665377, ECO:0007744\|PubMed:17330950, ECO:0007744\|PubMed:19779198

Chain	Residue	Details
A	SER315
B	SER315
D	SER315
C	SER315

site_id	SWS_FT_FI9
Number of Residues	20
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744\|PubMed:22106047

Chain	Residue	Details
A	LYS225
B	LYS318
D	LYS225
D	LYS233
D	LYS286
D	LYS318
C	LYS225
C	LYS233
C	LYS286
C	LYS318
A	LYS233
A	LYS286
A	LYS318
B	LYS225
B	LYS233
B	LYS286

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)