7KYA
Structure of the S. cerevisiae phosphatidylcholine flippase Dnf2-Lem3 complex in the E2P state
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0005215 | molecular_function | transporter activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005783 | cellular_component | endoplasmic reticulum |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0005935 | cellular_component | cellular bud neck |
| A | 0006869 | biological_process | lipid transport |
| A | 0006897 | biological_process | endocytosis |
| A | 0007163 | biological_process | establishment or maintenance of cell polarity |
| A | 0015031 | biological_process | protein transport |
| A | 0015247 | molecular_function | aminophospholipid flippase activity |
| A | 0015914 | biological_process | phospholipid transport |
| A | 0016020 | cellular_component | membrane |
| A | 0016887 | molecular_function | ATP hydrolysis activity |
| A | 0045332 | biological_process | phospholipid translocation |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0070867 | cellular_component | mating projection tip membrane |
| A | 0071944 | cellular_component | cell periphery |
| A | 0090554 | molecular_function | phosphatidylcholine floppase activity |
| A | 0090555 | molecular_function | phosphatidylethanolamine flippase activity |
| A | 0090556 | molecular_function | phosphatidylserine floppase activity |
| A | 0099040 | biological_process | ceramide translocation |
| A | 0140326 | molecular_function | ATPase-coupled intramembrane lipid transporter activity |
| A | 0140331 | biological_process | aminophospholipid translocation |
| A | 0140346 | molecular_function | phosphatidylserine flippase activity |
| A | 0140351 | molecular_function | glycosylceramide flippase activity |
| A | 1990531 | cellular_component | phospholipid-translocating ATPase complex |
| B | 0005515 | molecular_function | protein binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005783 | cellular_component | endoplasmic reticulum |
| B | 0005794 | cellular_component | Golgi apparatus |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0007166 | biological_process | cell surface receptor signaling pathway |
| B | 0015247 | molecular_function | aminophospholipid flippase activity |
| B | 0016020 | cellular_component | membrane |
| B | 0044088 | biological_process | regulation of vacuole organization |
| B | 0045332 | biological_process | phospholipid translocation |
| B | 0140331 | biological_process | aminophospholipid translocation |
| B | 0140345 | molecular_function | phosphatidylcholine flippase activity |
| B | 1990531 | cellular_component | phospholipid-translocating ATPase complex |
Functional Information from PROSITE/UniProt
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 93 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000305 |
| Chain | Residue | Details |
| B | MET1-THR74 | |
| B | THR394-LYS414 | |
| A | SER661-ILE1231 | |
| A | ASP1284-PHE1313 | |
| A | MET1372-TRP1377 | |
| A | ASP1440-ARG1612 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 40 |
| Details | TRANSMEM: Helical => ECO:0000255 |
| Chain | Residue | Details |
| B | VAL75-ALA95 | |
| A | PRO1419-ILE1439 | |
| B | PHE373-LEU393 | |
| A | ILE599-TYR619 | |
| A | SER640-ILE660 | |
| A | PRO1232-ASN1252 | |
| A | THR1263-LEU1283 | |
| A | LEU1314-ALA1334 | |
| A | ARG1351-PHE1371 | |
| A | ASP1378-ILE1398 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 276 |
| Details | TOPO_DOM: Extracellular => ECO:0000305 |
| Chain | Residue | Details |
| B | GLN96-PRO372 | |
| A | TYR620-ALA639 | |
| A | ASN1253-TYR1262 | |
| A | TYR1335-HIS1350 | |
| A | TRP1399-GLN1418 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198 |
| Chain | Residue | Details |
| B | SER36 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255 |
| Chain | Residue | Details |
| B | ASN113 | |
| B | ASN279 | |
| A | THR714 | |
| A | PHE887 | |
| A | ASP1173 | |
| A | ASN1176 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 3 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:33320091 |
| Chain | Residue | Details |
| B | ASN240 | |
| B | ASN298 | |
| B | ASN332 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:33320091, ECO:0007744|PDB:7KY5 |
| Chain | Residue | Details |
| B | ASN256 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P39524 |
| Chain | Residue | Details |
| A | LYS892 | |
| A | THR953 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 3 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P04191 |
| Chain | Residue | Details |
| A | LYS916 | |
| A | ASP1034 | |
| A | LYS1153 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:33320091, ECO:0007744|PDB:7KY8 |
| Chain | Residue | Details |
| A | ARG952 | |
| A | ARG1147 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:33320091, ECO:0007744|PDB:7KY8, ECO:0007744|PDB:7KY9 |
| Chain | Residue | Details |
| A | GLY1033 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:Q8NB49 |
| Chain | Residue | Details |
| A | ASP1177 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:G0S196 |
| Chain | Residue | Details |
| A | ARG1436 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 1 |
| Details | SITE: Involved in the release of the transported lipid into the cytosolic leaflet => ECO:0000250|UniProtKB:C7EXK4 |
| Chain | Residue | Details |
| A | ILE660 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphothreonine => ECO:0007744|PubMed:17330950 |
| Chain | Residue | Details |
| A | THR70 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 3 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:19779198 |
| Chain | Residue | Details |
| A | SER85 | |
| A | SER396 | |
| A | SER403 |
| site_id | SWS_FT_FI17 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:17330950 |
| Chain | Residue | Details |
| A | SER389 | |
| A | SER392 |
| site_id | SWS_FT_FI18 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:19779198 |
| Chain | Residue | Details |
| A | TYR406 |
| site_id | SWS_FT_FI19 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphothreonine => ECO:0007744|PubMed:19779198 |
| Chain | Residue | Details |
| A | THR782 |
| site_id | SWS_FT_FI20 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:15665377, ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198 |
| Chain | Residue | Details |
| A | SER1542 |
| site_id | SWS_FT_FI21 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198 |
| Chain | Residue | Details |
| A | SER1592 |
| site_id | SWS_FT_FI22 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047 |
| Chain | Residue | Details |
| A | LYS938 |
