Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7KWH

Spermidine N-acetyltransferase SpeG K23-Y30 chimera from Vibrio cholerae and hSSAT

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0004145	molecular_function	diamine N-acetyltransferase activity
A	0005737	cellular_component	cytoplasm
A	0006598	biological_process	polyamine catabolic process
A	0016746	molecular_function	acyltransferase activity
A	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
A	0046203	biological_process	spermidine catabolic process
A	0046208	biological_process	spermine catabolic process
A	0046872	molecular_function	metal ion binding
B	0000287	molecular_function	magnesium ion binding
B	0004145	molecular_function	diamine N-acetyltransferase activity
B	0005737	cellular_component	cytoplasm
B	0006598	biological_process	polyamine catabolic process
B	0016746	molecular_function	acyltransferase activity
B	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
B	0046203	biological_process	spermidine catabolic process
B	0046208	biological_process	spermine catabolic process
B	0046872	molecular_function	metal ion binding
C	0000287	molecular_function	magnesium ion binding
C	0004145	molecular_function	diamine N-acetyltransferase activity
C	0005737	cellular_component	cytoplasm
C	0006598	biological_process	polyamine catabolic process
C	0016746	molecular_function	acyltransferase activity
C	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
C	0046203	biological_process	spermidine catabolic process
C	0046208	biological_process	spermine catabolic process
C	0046872	molecular_function	metal ion binding
D	0000287	molecular_function	magnesium ion binding
D	0004145	molecular_function	diamine N-acetyltransferase activity
D	0005737	cellular_component	cytoplasm
D	0006598	biological_process	polyamine catabolic process
D	0016746	molecular_function	acyltransferase activity
D	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
D	0046203	biological_process	spermidine catabolic process
D	0046208	biological_process	spermine catabolic process
D	0046872	molecular_function	metal ion binding
E	0000287	molecular_function	magnesium ion binding
E	0004145	molecular_function	diamine N-acetyltransferase activity
E	0005737	cellular_component	cytoplasm
E	0006598	biological_process	polyamine catabolic process
E	0016746	molecular_function	acyltransferase activity
E	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
E	0046203	biological_process	spermidine catabolic process
E	0046208	biological_process	spermine catabolic process
E	0046872	molecular_function	metal ion binding
F	0000287	molecular_function	magnesium ion binding
F	0004145	molecular_function	diamine N-acetyltransferase activity
F	0005737	cellular_component	cytoplasm
F	0006598	biological_process	polyamine catabolic process
F	0016746	molecular_function	acyltransferase activity
F	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
F	0046203	biological_process	spermidine catabolic process
F	0046208	biological_process	spermine catabolic process
F	0046872	molecular_function	metal ion binding
G	0000287	molecular_function	magnesium ion binding
G	0004145	molecular_function	diamine N-acetyltransferase activity
G	0005737	cellular_component	cytoplasm
G	0006598	biological_process	polyamine catabolic process
G	0016746	molecular_function	acyltransferase activity
G	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
G	0046203	biological_process	spermidine catabolic process
G	0046208	biological_process	spermine catabolic process
G	0046872	molecular_function	metal ion binding
H	0000287	molecular_function	magnesium ion binding
H	0004145	molecular_function	diamine N-acetyltransferase activity
H	0005737	cellular_component	cytoplasm
H	0006598	biological_process	polyamine catabolic process
H	0016746	molecular_function	acyltransferase activity
H	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
H	0046203	biological_process	spermidine catabolic process
H	0046208	biological_process	spermine catabolic process
H	0046872	molecular_function	metal ion binding
I	0000287	molecular_function	magnesium ion binding
I	0004145	molecular_function	diamine N-acetyltransferase activity
I	0005737	cellular_component	cytoplasm
I	0006598	biological_process	polyamine catabolic process
I	0016746	molecular_function	acyltransferase activity
I	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
I	0046203	biological_process	spermidine catabolic process
I	0046208	biological_process	spermine catabolic process
I	0046872	molecular_function	metal ion binding
J	0000287	molecular_function	magnesium ion binding
J	0004145	molecular_function	diamine N-acetyltransferase activity
J	0005737	cellular_component	cytoplasm
J	0006598	biological_process	polyamine catabolic process
J	0016746	molecular_function	acyltransferase activity
J	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
J	0046203	biological_process	spermidine catabolic process
J	0046208	biological_process	spermine catabolic process
J	0046872	molecular_function	metal ion binding
K	0000287	molecular_function	magnesium ion binding
K	0004145	molecular_function	diamine N-acetyltransferase activity
K	0005737	cellular_component	cytoplasm
K	0006598	biological_process	polyamine catabolic process
K	0016746	molecular_function	acyltransferase activity
K	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
K	0046203	biological_process	spermidine catabolic process
K	0046208	biological_process	spermine catabolic process
K	0046872	molecular_function	metal ion binding
L	0000287	molecular_function	magnesium ion binding
L	0004145	molecular_function	diamine N-acetyltransferase activity
L	0005737	cellular_component	cytoplasm
L	0006598	biological_process	polyamine catabolic process
L	0016746	molecular_function	acyltransferase activity
L	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
L	0046203	biological_process	spermidine catabolic process
L	0046208	biological_process	spermine catabolic process
L	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	binding site for residue PO4 A 201

Chain	Residue
A	GLY95
A	LYS96
A	GLY97
A	PHE98
A	ALA99

site_id	AC2
Number of Residues	5
Details	binding site for residue PO4 B 201

Chain	Residue
B	ALA99
B	GLY95
B	LYS96
B	GLY97
B	PHE98

site_id	AC3
Number of Residues	3
Details	binding site for residue PO4 C 201

Chain	Residue
C	GLY95
C	GLY97
C	ALA99

site_id	AC4
Number of Residues	6
Details	binding site for residue PO4 D 201

Chain	Residue
D	GLY95
D	LYS96
D	GLY97
D	PHE98
D	ALA99
D	LEU133

site_id	AC5
Number of Residues	5
Details	binding site for residue PO4 F 201

Chain	Residue
F	GLY95
F	LYS96
F	GLY97
F	PHE98
F	ALA99

site_id	AC6
Number of Residues	5
Details	binding site for residue PO4 J 201

Chain	Residue
J	GLY95
J	LYS96
J	GLY97
J	PHE98
J	ALA99

site_id	AC7
Number of Residues	6
Details	binding site for residue PO4 K 201

Chain	Residue
K	GLY95
K	LYS96
K	GLY97
K	PHE98
K	ALA99
K	LEU133

site_id	AC8
Number of Residues	5
Details	binding site for residue PO4 H 201

Chain	Residue
H	GLY95
H	LYS96
H	GLY97
H	PHE98
H	ALA99

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	ACT_SITE: Proton donor => ECO:0000250\|UniProtKB:P0A951

Chain	Residue	Details
A	TYR134
G	TYR134
H	TYR134
I	TYR134
B	TYR134
C	TYR134
D	TYR134
E	TYR134
F	TYR134
J	TYR134
L	TYR134
K	TYR134

site_id	SWS_FT_FI2
Number of Residues	24
Details	BINDING: BINDING => ECO:0000269\|PubMed:25623305, ECO:0000269\|Ref.3, ECO:0007744\|PDB:4MJ8

Chain	Residue	Details
A	TYR28
E	GLU84
F	TYR28
F	GLU84
J	TYR28
J	GLU84
L	TYR28
L	GLU84
K	TYR28
K	GLU84
G	TYR28
A	GLU84
G	GLU84
H	TYR28
H	GLU84
I	TYR28
I	GLU84
B	TYR28
B	GLU84
C	TYR28
C	GLU84
D	TYR28
D	GLU84
E	TYR28

site_id	SWS_FT_FI3
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269\|PubMed:25623305, ECO:0007744\|PDB:4MI4

Chain	Residue	Details
A	GLU33
G	GLU33
H	GLU33
I	GLU33
B	GLU33
C	GLU33
D	GLU33
E	GLU33
F	GLU33
J	GLU33
L	GLU33
K	GLU33

site_id	SWS_FT_FI4
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269\|PubMed:25623305, ECO:0007744\|PDB:4MI4, ECO:0007744\|PDB:4R87

Chain	Residue	Details
A	GLU41
G	GLU41
H	GLU41
I	GLU41
B	GLU41
C	GLU41
D	GLU41
E	GLU41
F	GLU41
J	GLU41
L	GLU41
K	GLU41

site_id	SWS_FT_FI5
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269\|PubMed:25623305, ECO:0007744\|PDB:4R87

Chain	Residue	Details
A	HIS49
G	HIS49
H	HIS49
I	HIS49
B	HIS49
C	HIS49
D	HIS49
E	HIS49
F	HIS49
J	HIS49
L	HIS49
K	HIS49

site_id	SWS_FT_FI6
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269\|PubMed:25623305, ECO:0000269\|PubMed:26410587, ECO:0007744\|PDB:5CNP

Chain	Residue	Details
A	GLU75
G	GLU75
H	GLU75
I	GLU75
B	GLU75
C	GLU75
D	GLU75
E	GLU75
F	GLU75
J	GLU75
L	GLU75
K	GLU75

site_id	SWS_FT_FI7
Number of Residues	24
Details	BINDING: BINDING => ECO:0000269\|PubMed:25623305, ECO:0007744\|PDB:4R57, ECO:0007744\|PDB:4R87

Chain	Residue	Details
A	ILE87
E	GLN94
F	ILE87
F	GLN94
J	ILE87
J	GLN94
L	ILE87
L	GLN94
K	ILE87
K	GLN94
G	ILE87
A	GLN94
G	GLN94
H	ILE87
H	GLN94
I	ILE87
I	GLN94
B	ILE87
B	GLN94
C	ILE87
C	GLN94
D	ILE87
D	GLN94
E	ILE87

site_id	SWS_FT_FI8
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269\|PubMed:25623305, ECO:0007744\|PDB:4R57

Chain	Residue	Details
A	ASN127
G	ASN127
H	ASN127
I	ASN127
B	ASN127
C	ASN127
D	ASN127
E	ASN127
F	ASN127
J	ASN127
L	ASN127
K	ASN127

site_id	SWS_FT_FI9
Number of Residues	12
Details	SITE: Could be important for selectivity toward long polyamines => ECO:0000305\|PubMed:25623305

Chain	Residue	Details
A	GLU84
G	GLU84
H	GLU84
I	GLU84
B	GLU84
C	GLU84
D	GLU84
E	GLU84
F	GLU84
J	GLU84
L	GLU84
K	GLU84

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)