7KRG

Crystal Structure of Mannitol Dehydrogenase (ChMDH) from Cladosporium herbarum in complex with NADP+ and Na

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0016491	molecular_function	oxidoreductase activity
A	0019594	biological_process	mannitol metabolic process
A	0042803	molecular_function	protein homodimerization activity
A	0050085	molecular_function	mannitol 2-dehydrogenase (NADP+) activity
A	0050661	molecular_function	NADP binding
A	0050664	molecular_function	oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor
A	0051289	biological_process	protein homotetramerization
B	0016491	molecular_function	oxidoreductase activity
B	0019594	biological_process	mannitol metabolic process
B	0042803	molecular_function	protein homodimerization activity
B	0050085	molecular_function	mannitol 2-dehydrogenase (NADP+) activity
B	0050661	molecular_function	NADP binding
B	0050664	molecular_function	oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor
B	0051289	biological_process	protein homotetramerization
C	0016491	molecular_function	oxidoreductase activity
C	0019594	biological_process	mannitol metabolic process
C	0042803	molecular_function	protein homodimerization activity
C	0050085	molecular_function	mannitol 2-dehydrogenase (NADP+) activity
C	0050661	molecular_function	NADP binding
C	0050664	molecular_function	oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor
C	0051289	biological_process	protein homotetramerization
D	0016491	molecular_function	oxidoreductase activity
D	0019594	biological_process	mannitol metabolic process
D	0042803	molecular_function	protein homodimerization activity
D	0050085	molecular_function	mannitol 2-dehydrogenase (NADP+) activity
D	0050661	molecular_function	NADP binding
D	0050664	molecular_function	oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor
D	0051289	biological_process	protein homotetramerization
E	0016491	molecular_function	oxidoreductase activity
E	0019594	biological_process	mannitol metabolic process
E	0042803	molecular_function	protein homodimerization activity
E	0050085	molecular_function	mannitol 2-dehydrogenase (NADP+) activity
E	0050661	molecular_function	NADP binding
E	0050664	molecular_function	oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor
E	0051289	biological_process	protein homotetramerization
F	0016491	molecular_function	oxidoreductase activity
F	0019594	biological_process	mannitol metabolic process
F	0042803	molecular_function	protein homodimerization activity
F	0050085	molecular_function	mannitol 2-dehydrogenase (NADP+) activity
F	0050661	molecular_function	NADP binding
F	0050664	molecular_function	oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor
F	0051289	biological_process	protein homotetramerization
G	0016491	molecular_function	oxidoreductase activity
G	0019594	biological_process	mannitol metabolic process
G	0042803	molecular_function	protein homodimerization activity
G	0050085	molecular_function	mannitol 2-dehydrogenase (NADP+) activity
G	0050661	molecular_function	NADP binding
G	0050664	molecular_function	oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor
G	0051289	biological_process	protein homotetramerization
H	0016491	molecular_function	oxidoreductase activity
H	0019594	biological_process	mannitol metabolic process
H	0042803	molecular_function	protein homodimerization activity
H	0050085	molecular_function	mannitol 2-dehydrogenase (NADP+) activity
H	0050661	molecular_function	NADP binding
H	0050664	molecular_function	oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor
H	0051289	biological_process	protein homotetramerization

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: Proton donor => ECO:0000305|PubMed:20420880

Chain	Residue	Details
A	SER160
B	SER160
C	SER160
D	SER160
E	SER160
F	SER160
G	SER160
H	SER160

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site_id	SWS_FT_FI2
Number of Residues	8
Details	ACT_SITE: Proton acceptor => ECO:0000305|PubMed:20420880

Chain	Residue	Details
A	TYR175
B	TYR175
C	TYR175
D	TYR175
E	TYR175
F	TYR175
G	TYR175
H	TYR175

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site_id	SWS_FT_FI3
Number of Residues	8
Details	ACT_SITE: Lowers pKa of active site Tyr => ECO:0000305|PubMed:20420880

Chain	Residue	Details
B	LYS179
A	LYS179
C	LYS179
D	LYS179
E	LYS179
F	LYS179
G	LYS179
H	LYS179

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site_id	SWS_FT_FI4
Number of Residues	32
Details	BINDING: BINDING => ECO:0000250|UniProtKB:O93868

Chain	Residue	Details
D	ILE207
E	ASN108
E	TYR175
E	LYS179
E	ILE207
F	ASN108
F	TYR175
F	LYS179
F	ILE207
G	ASN108
G	TYR175
G	LYS179
G	ILE207
H	ASN108
H	TYR175
H	LYS179
H	ILE207
A	ASN108
C	TYR175
C	LYS179
C	ILE207
D	ASN108
D	TYR175
D	LYS179
A	TYR175
A	LYS179
A	ILE207
B	ASN108
B	TYR175
B	LYS179
B	ILE207
C	ASN108

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site_id	SWS_FT_FI5
Number of Residues	16
Details	BINDING: BINDING => ECO:0000250|UniProtKB:L0E2Z4

Chain	Residue	Details
A	LYS141
E	THR209
F	LYS141
F	THR209
G	LYS141
G	THR209
H	LYS141
H	THR209
A	THR209
B	LYS141
B	THR209
C	LYS141
C	THR209
D	LYS141
D	THR209
E	LYS141

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