7FQL

Crystal Structure of human Legumain in complex with (2S)-N-[(1S)-3-amino-1-cyano-3-oxopropyl]-1-[1-[4-[(2,4-difluorophenyl)methoxy]phenyl]cyclopropanecarbonyl]pyrrolidine-2-carboxamide

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003014	biological_process	renal system process
A	0004175	molecular_function	endopeptidase activity
A	0004197	molecular_function	cysteine-type endopeptidase activity
A	0005576	cellular_component	extracellular region
A	0005737	cellular_component	cytoplasm
A	0005764	cellular_component	lysosome
A	0005770	cellular_component	late endosome
A	0006508	biological_process	proteolysis
A	0006624	biological_process	vacuolar protein processing
A	0007613	biological_process	memory
A	0008233	molecular_function	peptidase activity
A	0008234	molecular_function	cysteine-type peptidase activity
A	0008284	biological_process	positive regulation of cell population proliferation
A	0008306	biological_process	associative learning
A	0010447	biological_process	response to acidic pH
A	0010629	biological_process	negative regulation of gene expression
A	0019886	biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class II
A	0032801	biological_process	receptor catabolic process
A	0035729	biological_process	cellular response to hepatocyte growth factor stimulus
A	0036021	cellular_component	endolysosome lumen
A	0040015	biological_process	negative regulation of multicellular organism growth
A	0042359	biological_process	vitamin D metabolic process
A	0043202	cellular_component	lysosomal lumen
A	0043524	biological_process	negative regulation of neuron apoptotic process
A	0045177	cellular_component	apical part of cell
A	0045931	biological_process	positive regulation of mitotic cell cycle
A	0048156	molecular_function	tau protein binding
A	0048471	cellular_component	perinuclear region of cytoplasm
A	0051603	biological_process	proteolysis involved in protein catabolic process
A	0051604	biological_process	protein maturation
A	0061133	molecular_function	endopeptidase activator activity
A	0070062	cellular_component	extracellular exosome
A	0071277	biological_process	cellular response to calcium ion
A	0090026	biological_process	positive regulation of monocyte chemotaxis
A	0097061	biological_process	dendritic spine organization
A	0097202	biological_process	activation of cysteine-type endopeptidase activity
A	0097264	biological_process	self proteolysis
A	1900273	biological_process	positive regulation of long-term synaptic potentiation
A	1901185	biological_process	negative regulation of ERBB signaling pathway
A	1904646	biological_process	cellular response to amyloid-beta
A	2001028	biological_process	positive regulation of endothelial cell chemotaxis
B	0003014	biological_process	renal system process
B	0004175	molecular_function	endopeptidase activity
B	0004197	molecular_function	cysteine-type endopeptidase activity
B	0005576	cellular_component	extracellular region
B	0005737	cellular_component	cytoplasm
B	0005764	cellular_component	lysosome
B	0005770	cellular_component	late endosome
B	0006508	biological_process	proteolysis
B	0006624	biological_process	vacuolar protein processing
B	0007613	biological_process	memory
B	0008233	molecular_function	peptidase activity
B	0008234	molecular_function	cysteine-type peptidase activity
B	0008284	biological_process	positive regulation of cell population proliferation
B	0008306	biological_process	associative learning
B	0010447	biological_process	response to acidic pH
B	0010629	biological_process	negative regulation of gene expression
B	0019886	biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class II
B	0032801	biological_process	receptor catabolic process
B	0035729	biological_process	cellular response to hepatocyte growth factor stimulus
B	0036021	cellular_component	endolysosome lumen
B	0040015	biological_process	negative regulation of multicellular organism growth
B	0042359	biological_process	vitamin D metabolic process
B	0043202	cellular_component	lysosomal lumen
B	0043524	biological_process	negative regulation of neuron apoptotic process
B	0045177	cellular_component	apical part of cell
B	0045931	biological_process	positive regulation of mitotic cell cycle
B	0048156	molecular_function	tau protein binding
B	0048471	cellular_component	perinuclear region of cytoplasm
B	0051603	biological_process	proteolysis involved in protein catabolic process
B	0051604	biological_process	protein maturation
B	0061133	molecular_function	endopeptidase activator activity
B	0070062	cellular_component	extracellular exosome
B	0071277	biological_process	cellular response to calcium ion
B	0090026	biological_process	positive regulation of monocyte chemotaxis
B	0097061	biological_process	dendritic spine organization
B	0097202	biological_process	activation of cysteine-type endopeptidase activity
B	0097264	biological_process	self proteolysis
B	1900273	biological_process	positive regulation of long-term synaptic potentiation
B	1901185	biological_process	negative regulation of ERBB signaling pathway
B	1904646	biological_process	cellular response to amyloid-beta
B	2001028	biological_process	positive regulation of endothelial cell chemotaxis
C	0003014	biological_process	renal system process
C	0004175	molecular_function	endopeptidase activity
C	0004197	molecular_function	cysteine-type endopeptidase activity
C	0005576	cellular_component	extracellular region
C	0005737	cellular_component	cytoplasm
C	0005764	cellular_component	lysosome
C	0005770	cellular_component	late endosome
C	0006508	biological_process	proteolysis
C	0006624	biological_process	vacuolar protein processing
C	0007613	biological_process	memory
C	0008233	molecular_function	peptidase activity
C	0008234	molecular_function	cysteine-type peptidase activity
C	0008284	biological_process	positive regulation of cell population proliferation
C	0008306	biological_process	associative learning
C	0010447	biological_process	response to acidic pH
C	0010629	biological_process	negative regulation of gene expression
C	0019886	biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class II
C	0032801	biological_process	receptor catabolic process
C	0035729	biological_process	cellular response to hepatocyte growth factor stimulus
C	0036021	cellular_component	endolysosome lumen
C	0040015	biological_process	negative regulation of multicellular organism growth
C	0042359	biological_process	vitamin D metabolic process
C	0043202	cellular_component	lysosomal lumen
C	0043524	biological_process	negative regulation of neuron apoptotic process
C	0045177	cellular_component	apical part of cell
C	0045931	biological_process	positive regulation of mitotic cell cycle
C	0048156	molecular_function	tau protein binding
C	0048471	cellular_component	perinuclear region of cytoplasm
C	0051603	biological_process	proteolysis involved in protein catabolic process
C	0051604	biological_process	protein maturation
C	0061133	molecular_function	endopeptidase activator activity
C	0070062	cellular_component	extracellular exosome
C	0071277	biological_process	cellular response to calcium ion
C	0090026	biological_process	positive regulation of monocyte chemotaxis
C	0097061	biological_process	dendritic spine organization
C	0097202	biological_process	activation of cysteine-type endopeptidase activity
C	0097264	biological_process	self proteolysis
C	1900273	biological_process	positive regulation of long-term synaptic potentiation
C	1901185	biological_process	negative regulation of ERBB signaling pathway
C	1904646	biological_process	cellular response to amyloid-beta
C	2001028	biological_process	positive regulation of endothelial cell chemotaxis
D	0003014	biological_process	renal system process
D	0004175	molecular_function	endopeptidase activity
D	0004197	molecular_function	cysteine-type endopeptidase activity
D	0005576	cellular_component	extracellular region
D	0005737	cellular_component	cytoplasm
D	0005764	cellular_component	lysosome
D	0005770	cellular_component	late endosome
D	0006508	biological_process	proteolysis
D	0006624	biological_process	vacuolar protein processing
D	0007613	biological_process	memory
D	0008233	molecular_function	peptidase activity
D	0008234	molecular_function	cysteine-type peptidase activity
D	0008284	biological_process	positive regulation of cell population proliferation
D	0008306	biological_process	associative learning
D	0010447	biological_process	response to acidic pH
D	0010629	biological_process	negative regulation of gene expression
D	0019886	biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class II
D	0032801	biological_process	receptor catabolic process
D	0035729	biological_process	cellular response to hepatocyte growth factor stimulus
D	0036021	cellular_component	endolysosome lumen
D	0040015	biological_process	negative regulation of multicellular organism growth
D	0042359	biological_process	vitamin D metabolic process
D	0043202	cellular_component	lysosomal lumen
D	0043524	biological_process	negative regulation of neuron apoptotic process
D	0045177	cellular_component	apical part of cell
D	0045931	biological_process	positive regulation of mitotic cell cycle
D	0048156	molecular_function	tau protein binding
D	0048471	cellular_component	perinuclear region of cytoplasm
D	0051603	biological_process	proteolysis involved in protein catabolic process
D	0051604	biological_process	protein maturation
D	0061133	molecular_function	endopeptidase activator activity
D	0070062	cellular_component	extracellular exosome
D	0071277	biological_process	cellular response to calcium ion
D	0090026	biological_process	positive regulation of monocyte chemotaxis
D	0097061	biological_process	dendritic spine organization
D	0097202	biological_process	activation of cysteine-type endopeptidase activity
D	0097264	biological_process	self proteolysis
D	1900273	biological_process	positive regulation of long-term synaptic potentiation
D	1901185	biological_process	negative regulation of ERBB signaling pathway
D	1904646	biological_process	cellular response to amyloid-beta
D	2001028	biological_process	positive regulation of endothelial cell chemotaxis
E	0003014	biological_process	renal system process
E	0004175	molecular_function	endopeptidase activity
E	0004197	molecular_function	cysteine-type endopeptidase activity
E	0005576	cellular_component	extracellular region
E	0005737	cellular_component	cytoplasm
E	0005764	cellular_component	lysosome
E	0005770	cellular_component	late endosome
E	0006508	biological_process	proteolysis
E	0006624	biological_process	vacuolar protein processing
E	0007613	biological_process	memory
E	0008233	molecular_function	peptidase activity
E	0008234	molecular_function	cysteine-type peptidase activity
E	0008284	biological_process	positive regulation of cell population proliferation
E	0008306	biological_process	associative learning
E	0010447	biological_process	response to acidic pH
E	0010629	biological_process	negative regulation of gene expression
E	0019886	biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class II
E	0032801	biological_process	receptor catabolic process
E	0035729	biological_process	cellular response to hepatocyte growth factor stimulus
E	0036021	cellular_component	endolysosome lumen
E	0040015	biological_process	negative regulation of multicellular organism growth
E	0042359	biological_process	vitamin D metabolic process
E	0043202	cellular_component	lysosomal lumen
E	0043524	biological_process	negative regulation of neuron apoptotic process
E	0045177	cellular_component	apical part of cell
E	0045931	biological_process	positive regulation of mitotic cell cycle
E	0048156	molecular_function	tau protein binding
E	0048471	cellular_component	perinuclear region of cytoplasm
E	0051603	biological_process	proteolysis involved in protein catabolic process
E	0051604	biological_process	protein maturation
E	0061133	molecular_function	endopeptidase activator activity
E	0070062	cellular_component	extracellular exosome
E	0071277	biological_process	cellular response to calcium ion
E	0090026	biological_process	positive regulation of monocyte chemotaxis
E	0097061	biological_process	dendritic spine organization
E	0097202	biological_process	activation of cysteine-type endopeptidase activity
E	0097264	biological_process	self proteolysis
E	1900273	biological_process	positive regulation of long-term synaptic potentiation
E	1901185	biological_process	negative regulation of ERBB signaling pathway
E	1904646	biological_process	cellular response to amyloid-beta
E	2001028	biological_process	positive regulation of endothelial cell chemotaxis
F	0003014	biological_process	renal system process
F	0004175	molecular_function	endopeptidase activity
F	0004197	molecular_function	cysteine-type endopeptidase activity
F	0005576	cellular_component	extracellular region
F	0005737	cellular_component	cytoplasm
F	0005764	cellular_component	lysosome
F	0005770	cellular_component	late endosome
F	0006508	biological_process	proteolysis
F	0006624	biological_process	vacuolar protein processing
F	0007613	biological_process	memory
F	0008233	molecular_function	peptidase activity
F	0008234	molecular_function	cysteine-type peptidase activity
F	0008284	biological_process	positive regulation of cell population proliferation
F	0008306	biological_process	associative learning
F	0010447	biological_process	response to acidic pH
F	0010629	biological_process	negative regulation of gene expression
F	0019886	biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class II
F	0032801	biological_process	receptor catabolic process
F	0035729	biological_process	cellular response to hepatocyte growth factor stimulus
F	0036021	cellular_component	endolysosome lumen
F	0040015	biological_process	negative regulation of multicellular organism growth
F	0042359	biological_process	vitamin D metabolic process
F	0043202	cellular_component	lysosomal lumen
F	0043524	biological_process	negative regulation of neuron apoptotic process
F	0045177	cellular_component	apical part of cell
F	0045931	biological_process	positive regulation of mitotic cell cycle
F	0048156	molecular_function	tau protein binding
F	0048471	cellular_component	perinuclear region of cytoplasm
F	0051603	biological_process	proteolysis involved in protein catabolic process
F	0051604	biological_process	protein maturation
F	0061133	molecular_function	endopeptidase activator activity
F	0070062	cellular_component	extracellular exosome
F	0071277	biological_process	cellular response to calcium ion
F	0090026	biological_process	positive regulation of monocyte chemotaxis
F	0097061	biological_process	dendritic spine organization
F	0097202	biological_process	activation of cysteine-type endopeptidase activity
F	0097264	biological_process	self proteolysis
F	1900273	biological_process	positive regulation of long-term synaptic potentiation
F	1901185	biological_process	negative regulation of ERBB signaling pathway
F	1904646	biological_process	cellular response to amyloid-beta
F	2001028	biological_process	positive regulation of endothelial cell chemotaxis
G	0003014	biological_process	renal system process
G	0004175	molecular_function	endopeptidase activity
G	0004197	molecular_function	cysteine-type endopeptidase activity
G	0005576	cellular_component	extracellular region
G	0005737	cellular_component	cytoplasm
G	0005764	cellular_component	lysosome
G	0005770	cellular_component	late endosome
G	0006508	biological_process	proteolysis
G	0006624	biological_process	vacuolar protein processing
G	0007613	biological_process	memory
G	0008233	molecular_function	peptidase activity
G	0008234	molecular_function	cysteine-type peptidase activity
G	0008284	biological_process	positive regulation of cell population proliferation
G	0008306	biological_process	associative learning
G	0010447	biological_process	response to acidic pH
G	0010629	biological_process	negative regulation of gene expression
G	0019886	biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class II
G	0032801	biological_process	receptor catabolic process
G	0035729	biological_process	cellular response to hepatocyte growth factor stimulus
G	0036021	cellular_component	endolysosome lumen
G	0040015	biological_process	negative regulation of multicellular organism growth
G	0042359	biological_process	vitamin D metabolic process
G	0043202	cellular_component	lysosomal lumen
G	0043524	biological_process	negative regulation of neuron apoptotic process
G	0045177	cellular_component	apical part of cell
G	0045931	biological_process	positive regulation of mitotic cell cycle
G	0048156	molecular_function	tau protein binding
G	0048471	cellular_component	perinuclear region of cytoplasm
G	0051603	biological_process	proteolysis involved in protein catabolic process
G	0051604	biological_process	protein maturation
G	0061133	molecular_function	endopeptidase activator activity
G	0070062	cellular_component	extracellular exosome
G	0071277	biological_process	cellular response to calcium ion
G	0090026	biological_process	positive regulation of monocyte chemotaxis
G	0097061	biological_process	dendritic spine organization
G	0097202	biological_process	activation of cysteine-type endopeptidase activity
G	0097264	biological_process	self proteolysis
G	1900273	biological_process	positive regulation of long-term synaptic potentiation
G	1901185	biological_process	negative regulation of ERBB signaling pathway
G	1904646	biological_process	cellular response to amyloid-beta
G	2001028	biological_process	positive regulation of endothelial cell chemotaxis
H	0003014	biological_process	renal system process
H	0004175	molecular_function	endopeptidase activity
H	0004197	molecular_function	cysteine-type endopeptidase activity
H	0005576	cellular_component	extracellular region
H	0005737	cellular_component	cytoplasm
H	0005764	cellular_component	lysosome
H	0005770	cellular_component	late endosome
H	0006508	biological_process	proteolysis
H	0006624	biological_process	vacuolar protein processing
H	0007613	biological_process	memory
H	0008233	molecular_function	peptidase activity
H	0008234	molecular_function	cysteine-type peptidase activity
H	0008284	biological_process	positive regulation of cell population proliferation
H	0008306	biological_process	associative learning
H	0010447	biological_process	response to acidic pH
H	0010629	biological_process	negative regulation of gene expression
H	0019886	biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class II
H	0032801	biological_process	receptor catabolic process
H	0035729	biological_process	cellular response to hepatocyte growth factor stimulus
H	0036021	cellular_component	endolysosome lumen
H	0040015	biological_process	negative regulation of multicellular organism growth
H	0042359	biological_process	vitamin D metabolic process
H	0043202	cellular_component	lysosomal lumen
H	0043524	biological_process	negative regulation of neuron apoptotic process
H	0045177	cellular_component	apical part of cell
H	0045931	biological_process	positive regulation of mitotic cell cycle
H	0048156	molecular_function	tau protein binding
H	0048471	cellular_component	perinuclear region of cytoplasm
H	0051603	biological_process	proteolysis involved in protein catabolic process
H	0051604	biological_process	protein maturation
H	0061133	molecular_function	endopeptidase activator activity
H	0070062	cellular_component	extracellular exosome
H	0071277	biological_process	cellular response to calcium ion
H	0090026	biological_process	positive regulation of monocyte chemotaxis
H	0097061	biological_process	dendritic spine organization
H	0097202	biological_process	activation of cysteine-type endopeptidase activity
H	0097264	biological_process	self proteolysis
H	1900273	biological_process	positive regulation of long-term synaptic potentiation
H	1901185	biological_process	negative regulation of ERBB signaling pathway
H	1904646	biological_process	cellular response to amyloid-beta
H	2001028	biological_process	positive regulation of endothelial cell chemotaxis

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: ACT_SITE => ECO:0000305|PubMed:23776206

Chain	Residue	Details
A	HIS148
B	HIS148
C	HIS148
D	HIS148
E	HIS148
F	HIS148
G	HIS148
H	HIS148

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	ACT_SITE: Nucleophile => ECO:0000269|PubMed:23776206

Chain	Residue	Details
A	CYS189
B	CYS189
C	CYS189
D	CYS189
E	CYS189
F	CYS189
G	CYS189
H	CYS189

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	SITE: Cleavage; by autolysis => ECO:0000269|PubMed:11085925

Chain	Residue	Details
A	ASN323
B	ASN323
C	ASN323
D	ASN323
E	ASN323
F	ASN323
G	ASN323
H	ASN323

---

site_id	SWS_FT_FI4
Number of Residues	16
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:19159218, ECO:0000269|PubMed:23776206

Chain	Residue	Details
A	ASN91
E	ASN167
F	ASN91
F	ASN167
G	ASN91
G	ASN167
H	ASN91
H	ASN167
A	ASN167
B	ASN91
B	ASN167
C	ASN91
C	ASN167
D	ASN91
D	ASN167
E	ASN91

---

site_id	SWS_FT_FI5
Number of Residues	16
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:23776206

Chain	Residue	Details
A	ASN263
E	GLN272
F	ASN263
F	GLN272
G	ASN263
G	GLN272
H	ASN263
H	GLN272
A	GLN272
B	ASN263
B	GLN272
C	ASN263
C	GLN272
D	ASN263
D	GLN272
E	ASN263

---