7ENY

Crystal structure of hydroxysteroid dehydrogenase from Escherichia coli

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005829	cellular_component	cytosol
A	0006629	biological_process	lipid metabolic process
A	0008709	molecular_function	cholate 7-alpha-dehydrogenase (NAD+) activity
A	0030573	biological_process	bile acid catabolic process
A	0032787	biological_process	monocarboxylic acid metabolic process
A	0032991	cellular_component	protein-containing complex
A	0042802	molecular_function	identical protein binding
A	0051287	molecular_function	NAD binding
A	0106281	molecular_function	chenodeoxycholate 7-alpha-dehydrogenase (NAD+) activity
B	0005829	cellular_component	cytosol
B	0006629	biological_process	lipid metabolic process
B	0008709	molecular_function	cholate 7-alpha-dehydrogenase (NAD+) activity
B	0030573	biological_process	bile acid catabolic process
B	0032787	biological_process	monocarboxylic acid metabolic process
B	0032991	cellular_component	protein-containing complex
B	0042802	molecular_function	identical protein binding
B	0051287	molecular_function	NAD binding
B	0106281	molecular_function	chenodeoxycholate 7-alpha-dehydrogenase (NAD+) activity
C	0005829	cellular_component	cytosol
C	0006629	biological_process	lipid metabolic process
C	0008709	molecular_function	cholate 7-alpha-dehydrogenase (NAD+) activity
C	0030573	biological_process	bile acid catabolic process
C	0032787	biological_process	monocarboxylic acid metabolic process
C	0032991	cellular_component	protein-containing complex
C	0042802	molecular_function	identical protein binding
C	0051287	molecular_function	NAD binding
C	0106281	molecular_function	chenodeoxycholate 7-alpha-dehydrogenase (NAD+) activity
D	0005829	cellular_component	cytosol
D	0006629	biological_process	lipid metabolic process
D	0008709	molecular_function	cholate 7-alpha-dehydrogenase (NAD+) activity
D	0030573	biological_process	bile acid catabolic process
D	0032787	biological_process	monocarboxylic acid metabolic process
D	0032991	cellular_component	protein-containing complex
D	0042802	molecular_function	identical protein binding
D	0051287	molecular_function	NAD binding
D	0106281	molecular_function	chenodeoxycholate 7-alpha-dehydrogenase (NAD+) activity
E	0005829	cellular_component	cytosol
E	0006629	biological_process	lipid metabolic process
E	0008709	molecular_function	cholate 7-alpha-dehydrogenase (NAD+) activity
E	0030573	biological_process	bile acid catabolic process
E	0032787	biological_process	monocarboxylic acid metabolic process
E	0032991	cellular_component	protein-containing complex
E	0042802	molecular_function	identical protein binding
E	0051287	molecular_function	NAD binding
E	0106281	molecular_function	chenodeoxycholate 7-alpha-dehydrogenase (NAD+) activity
F	0005829	cellular_component	cytosol
F	0006629	biological_process	lipid metabolic process
F	0008709	molecular_function	cholate 7-alpha-dehydrogenase (NAD+) activity
F	0030573	biological_process	bile acid catabolic process
F	0032787	biological_process	monocarboxylic acid metabolic process
F	0032991	cellular_component	protein-containing complex
F	0042802	molecular_function	identical protein binding
F	0051287	molecular_function	NAD binding
F	0106281	molecular_function	chenodeoxycholate 7-alpha-dehydrogenase (NAD+) activity
G	0005829	cellular_component	cytosol
G	0006629	biological_process	lipid metabolic process
G	0008709	molecular_function	cholate 7-alpha-dehydrogenase (NAD+) activity
G	0030573	biological_process	bile acid catabolic process
G	0032787	biological_process	monocarboxylic acid metabolic process
G	0032991	cellular_component	protein-containing complex
G	0042802	molecular_function	identical protein binding
G	0051287	molecular_function	NAD binding
G	0106281	molecular_function	chenodeoxycholate 7-alpha-dehydrogenase (NAD+) activity
H	0005829	cellular_component	cytosol
H	0006629	biological_process	lipid metabolic process
H	0008709	molecular_function	cholate 7-alpha-dehydrogenase (NAD+) activity
H	0030573	biological_process	bile acid catabolic process
H	0032787	biological_process	monocarboxylic acid metabolic process
H	0032991	cellular_component	protein-containing complex
H	0042802	molecular_function	identical protein binding
H	0051287	molecular_function	NAD binding
H	0106281	molecular_function	chenodeoxycholate 7-alpha-dehydrogenase (NAD+) activity

Functional Information from PROSITE/UniProt

site_id	PS00061
Number of Residues	29
Details	ADH_SHORT Short-chain dehydrogenases/reductases family signature. SmaaenkninMtsYASSKAAAsHLVrNMA

Chain	Residue	Details
A	SER146-ALA174

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PubMed","id":"8672472","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"9722677","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	35
Details	Binding site: {"evidences":[{"source":"PubMed","id":"8672472","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1AHI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1FMC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	48
Details	Binding site: {"evidences":[{"source":"PubMed","id":"8672472","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1AHH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1AHI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1FMC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI4
Number of Residues	8
Details	Site: {"description":"Transition state stabilizer","evidences":[{"source":"PubMed","id":"8672472","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"9722677","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI5
Number of Residues	8
Details	Site: {"description":"Lowers pKa of active site Tyr","evidences":[{"source":"PubMed","id":"8672472","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"9722677","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

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