7DSI
Cryo-EM structure of Dnf1 from Saccharomyces cerevisiae in yeast lipids with AMPPCP ( resting state )
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0005215 | molecular_function | transporter activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005768 | cellular_component | endosome |
| A | 0005783 | cellular_component | endoplasmic reticulum |
| A | 0005794 | cellular_component | Golgi apparatus |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0005933 | cellular_component | cellular bud |
| A | 0005935 | cellular_component | cellular bud neck |
| A | 0006869 | biological_process | lipid transport |
| A | 0006886 | biological_process | intracellular protein transport |
| A | 0006897 | biological_process | endocytosis |
| A | 0007163 | biological_process | establishment or maintenance of cell polarity |
| A | 0010008 | cellular_component | endosome membrane |
| A | 0015914 | biological_process | phospholipid transport |
| A | 0016020 | cellular_component | membrane |
| A | 0016887 | molecular_function | ATP hydrolysis activity |
| A | 0030428 | cellular_component | cell septum |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0045332 | biological_process | phospholipid translocation |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0070867 | cellular_component | mating projection tip membrane |
| A | 0071944 | cellular_component | cell periphery |
| A | 0090554 | molecular_function | phosphatidylcholine floppase activity |
| A | 0090555 | molecular_function | phosphatidylethanolamine flippase activity |
| A | 0090556 | molecular_function | phosphatidylserine floppase activity |
| A | 0099040 | biological_process | ceramide translocation |
| A | 0140326 | molecular_function | ATPase-coupled intramembrane lipid transporter activity |
| A | 0140331 | biological_process | aminophospholipid translocation |
| A | 0140345 | molecular_function | phosphatidylcholine flippase activity |
| A | 0140346 | molecular_function | phosphatidylserine flippase activity |
| A | 0140351 | molecular_function | glycosylceramide flippase activity |
| A | 1990531 | cellular_component | phospholipid-translocating ATPase complex |
| B | 0005515 | molecular_function | protein binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005783 | cellular_component | endoplasmic reticulum |
| B | 0005794 | cellular_component | Golgi apparatus |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0007166 | biological_process | cell surface receptor signaling pathway |
| B | 0015247 | molecular_function | aminophospholipid flippase activity |
| B | 0016020 | cellular_component | membrane |
| B | 0044088 | biological_process | regulation of vacuole organization |
| B | 0045332 | biological_process | phospholipid translocation |
| B | 0140331 | biological_process | aminophospholipid translocation |
| B | 0140345 | molecular_function | phosphatidylcholine flippase activity |
| B | 1990531 | cellular_component | phospholipid-translocating ATPase complex |
Functional Information from PROSITE/UniProt
| site_id | PS00154 |
| Number of Residues | 7 |
| Details | ATPASE_E1_E2 E1-E2 ATPases phosphorylation site. DKTGTLT |
| Chain | Residue | Details |
| A | ASP667-THR673 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 93 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000305 |
| Chain | Residue | Details |
| B | MET1-THR74 | |
| B | THR394-LYS414 | |
| A | SER616-ILE1188 | |
| A | ASP1241-PHE1270 | |
| A | LEU1329-TRP1334 | |
| A | ASP1397-ASN1571 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 40 |
| Details | TRANSMEM: Helical => ECO:0000255 |
| Chain | Residue | Details |
| B | VAL75-ALA95 | |
| A | PRO1376-TYR1396 | |
| B | PHE373-LEU393 | |
| A | VAL554-TYR574 | |
| A | ALA595-ILE615 | |
| A | PRO1189-ASN1209 | |
| A | THR1220-LEU1240 | |
| A | LEU1271-VAL1291 | |
| A | ARG1308-LEU1328 | |
| A | ASP1335-ILE1355 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 276 |
| Details | TOPO_DOM: Extracellular => ECO:0000305 |
| Chain | Residue | Details |
| B | GLN96-PRO372 | |
| A | TYR575-ALA594 | |
| A | ASP1210-TYR1219 | |
| A | TYR1292-HIS1307 | |
| A | TRP1356-ALA1375 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198 |
| Chain | Residue | Details |
| B | SER36 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255 |
| Chain | Residue | Details |
| B | ASN113 | |
| B | ASN279 | |
| A | PHE842 | |
| A | ASP1130 | |
| A | ASN1133 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 3 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:33320091 |
| Chain | Residue | Details |
| B | ASN240 | |
| B | ASN298 | |
| B | ASN332 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:33320091, ECO:0007744|PDB:7KY5 |
| Chain | Residue | Details |
| B | ASN256 | |
| A | ARG1104 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:33320091, ECO:0000269|PubMed:35294892, ECO:0007744|PDB:7DSH, ECO:0007744|PDB:7DSI, ECO:0007744|PDB:7KYB, ECO:0007744|PDB:7WHW |
| Chain | Residue | Details |
| A | SER844 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P39524 |
| Chain | Residue | Details |
| A | LYS847 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 3 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P04191 |
| Chain | Residue | Details |
| A | LYS871 | |
| A | GLY990 | |
| A | ASP991 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:35294892, ECO:0007744|PDB:7DSH |
| Chain | Residue | Details |
| A | THR910 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:35294892, ECO:0007744|PDB:7DSH, ECO:0007744|PDB:7WHW |
| Chain | Residue | Details |
| A | THR989 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:Q8NB49 |
| Chain | Residue | Details |
| A | ASP1134 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:G0S196 |
| Chain | Residue | Details |
| A | ARG1393 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 1 |
| Details | SITE: Involved in the release of the transported lipid into the cytosolic leaflet => ECO:0000250|UniProtKB:C7EXK4 |
| Chain | Residue | Details |
| A | ILE615 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:17287358, ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198 |
| Chain | Residue | Details |
| A | SER53 |
| site_id | SWS_FT_FI17 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphothreonine => ECO:0007744|PubMed:19779198 |
| Chain | Residue | Details |
| A | THR70 | |
| A | THR94 | |
| A | THR109 | |
| A | THR1551 |
| site_id | SWS_FT_FI18 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:19779198 |
| Chain | Residue | Details |
| A | SER81 |
| site_id | SWS_FT_FI19 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphothreonine => ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:19779198 |
| Chain | Residue | Details |
| A | THR85 |
| site_id | SWS_FT_FI20 |
| Number of Residues | 5 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:19779198 |
| Chain | Residue | Details |
| A | SER92 | |
| A | SER104 | |
| A | SER365 | |
| A | SER1552 | |
| A | SER1563 |
| site_id | SWS_FT_FI21 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:18407956 |
| Chain | Residue | Details |
| A | SER351 |
| site_id | SWS_FT_FI22 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q12675 |
| Chain | Residue | Details |
| A | SER354 | |
| A | SER358 |
| site_id | SWS_FT_FI23 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:Q12675 |
| Chain | Residue | Details |
| A | TYR368 |
| site_id | SWS_FT_FI24 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198 |
| Chain | Residue | Details |
| A | SER1506 |
| site_id | SWS_FT_FI25 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000250|UniProtKB:Q12675 |
| Chain | Residue | Details |
| A | LYS895 |
