7CUB
2.55-Angstrom Cryo-EM structure of Cytochrome bo3 from Escherichia coli in Native Membrane
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004129 | molecular_function | cytochrome-c oxidase activity |
| A | 0005507 | molecular_function | copper ion binding |
| A | 0005515 | molecular_function | protein binding |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0009055 | molecular_function | electron transfer activity |
| A | 0009060 | biological_process | aerobic respiration |
| A | 0009319 | cellular_component | cytochrome o ubiquinol oxidase complex |
| A | 0009486 | molecular_function | cytochrome bo3 ubiquinol oxidase activity |
| A | 0015078 | molecular_function | proton transmembrane transporter activity |
| A | 0015453 | molecular_function | oxidoreduction-driven active transmembrane transporter activity |
| A | 0015990 | biological_process | electron transport coupled proton transport |
| A | 0016020 | cellular_component | membrane |
| A | 0016682 | molecular_function | oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor |
| A | 0019646 | biological_process | aerobic electron transport chain |
| A | 0020037 | molecular_function | heme binding |
| A | 0022904 | biological_process | respiratory electron transport chain |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0048039 | molecular_function | ubiquinone binding |
| A | 0070469 | cellular_component | respirasome |
| A | 1902600 | biological_process | proton transmembrane transport |
| B | 0004129 | molecular_function | cytochrome-c oxidase activity |
| B | 0005507 | molecular_function | copper ion binding |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0009055 | molecular_function | electron transfer activity |
| B | 0009060 | biological_process | aerobic respiration |
| B | 0009319 | cellular_component | cytochrome o ubiquinol oxidase complex |
| B | 0009486 | molecular_function | cytochrome bo3 ubiquinol oxidase activity |
| B | 0015078 | molecular_function | proton transmembrane transporter activity |
| B | 0015453 | molecular_function | oxidoreduction-driven active transmembrane transporter activity |
| B | 0015990 | biological_process | electron transport coupled proton transport |
| B | 0016020 | cellular_component | membrane |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016682 | molecular_function | oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor |
| B | 0019646 | biological_process | aerobic electron transport chain |
| B | 0022900 | biological_process | electron transport chain |
| B | 0042773 | biological_process | ATP synthesis coupled electron transport |
| B | 0070469 | cellular_component | respirasome |
| C | 0004129 | molecular_function | cytochrome-c oxidase activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005886 | cellular_component | plasma membrane |
| C | 0009055 | molecular_function | electron transfer activity |
| C | 0009060 | biological_process | aerobic respiration |
| C | 0009319 | cellular_component | cytochrome o ubiquinol oxidase complex |
| C | 0009486 | molecular_function | cytochrome bo3 ubiquinol oxidase activity |
| C | 0015078 | molecular_function | proton transmembrane transporter activity |
| C | 0015453 | molecular_function | oxidoreduction-driven active transmembrane transporter activity |
| C | 0015990 | biological_process | electron transport coupled proton transport |
| C | 0016020 | cellular_component | membrane |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0019646 | biological_process | aerobic electron transport chain |
| C | 0022904 | biological_process | respiratory electron transport chain |
| D | 0005515 | molecular_function | protein binding |
| D | 0005886 | cellular_component | plasma membrane |
| D | 0009055 | molecular_function | electron transfer activity |
| D | 0009060 | biological_process | aerobic respiration |
| D | 0009319 | cellular_component | cytochrome o ubiquinol oxidase complex |
| D | 0009486 | molecular_function | cytochrome bo3 ubiquinol oxidase activity |
| D | 0015078 | molecular_function | proton transmembrane transporter activity |
| D | 0015453 | molecular_function | oxidoreduction-driven active transmembrane transporter activity |
| D | 0015990 | biological_process | electron transport coupled proton transport |
| D | 0016020 | cellular_component | membrane |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0019646 | biological_process | aerobic electron transport chain |
Functional Information from PROSITE/UniProt
| site_id | PS00077 |
| Number of Residues | 55 |
| Details | COX1_CUB Heme-copper oxidase catalytic subunit, copper B binding region signature. WAWGHPeVyililpvfgvfseiaatfsrkrlfgytslvwatvcitvlsfivwl..HH |
| Chain | Residue | Details |
| A | TRP280-HIS334 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 31 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000305 |
| Chain | Residue | Details |
| D | MET1-THR17 | |
| D | PHE65-ALA80 | |
| C | VAL162-TRP184 | |
| A | THR261-GLY270 | |
| A | SER327-ALA340 | |
| A | LEU410-ASN412 | |
| A | GLY478-PRO489 | |
| A | ILE607-LEU613 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 54 |
| Details | TRANSMEM: Helical => ECO:0000305 |
| Chain | Residue | Details |
| D | TYR18-VAL36 | |
| D | ILE46-CYS64 | |
| D | PHE81-MET99 | |
| C | VAL143-MET161 | |
| C | HIS185-ALA203 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 17 |
| Details | TOPO_DOM: Periplasmic => ECO:0000305 |
| Chain | Residue | Details |
| D | MET37-VAL45 | |
| D | TRP100-HIS109 | |
| A | LYS308-PHE311 | |
| A | MET370-PHE377 | |
| A | PHE446-LEU448 | |
| A | ASP522-LYS587 | |
| A | ASP633-ASN663 | |
| C | MET204 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | LIPID: S-diacylglycerol cysteine => ECO:0000255|PROSITE-ProRule:PRU00303 |
| Chain | Residue | Details |
| B | CYS1 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 36 |
| Details | TRANSMEM: Helical; Name=III |
| Chain | Residue | Details |
| A | PRO96-GLY132 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 23 |
| Details | TRANSMEM: Helical; Name=IV |
| Chain | Residue | Details |
| A | PHE138-GLY161 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 30 |
| Details | TRANSMEM: Helical; Name=V |
| Chain | Residue | Details |
| A | VAL185-MET215 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 35 |
| Details | TRANSMEM: Helical; Name=VI |
| Chain | Residue | Details |
| A | MET225-GLY260 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 36 |
| Details | TRANSMEM: Helical; Name=VII |
| Chain | Residue | Details |
| A | ASN271-ARG307 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 14 |
| Details | TRANSMEM: Helical; Name=VIII |
| Chain | Residue | Details |
| A | GLY312-LEU326 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 28 |
| Details | TRANSMEM: Helical; Name=IX |
| Chain | Residue | Details |
| A | GLY341-THR369 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 31 |
| Details | TRANSMEM: Helical; Name=X |
| Chain | Residue | Details |
| A | HIS378-VAL409 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 32 |
| Details | TRANSMEM: Helical; Name=XI |
| Chain | Residue | Details |
| A | SER413-GLY445 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 28 |
| Details | TRANSMEM: Helical; Name=XII |
| Chain | Residue | Details |
| A | ASN449-MET477 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 31 |
| Details | TRANSMEM: Helical; Name=XIII |
| Chain | Residue | Details |
| A | GLN490-ARG521 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 18 |
| Details | TRANSMEM: Helical; Name=XIV |
| Chain | Residue | Details |
| A | ASN588-MET606 |
| site_id | SWS_FT_FI17 |
| Number of Residues | 18 |
| Details | TRANSMEM: Helical; Name=XV |
| Chain | Residue | Details |
| A | ALA614-GLU632 |
| site_id | SWS_FT_FI18 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:33408407, ECO:0000269|PubMed:34417297, ECO:0007744|PDB:6WTI, ECO:0007744|PDB:7CUB, ECO:0007744|PDB:7CUW, ECO:0007744|PDB:7N9Z |
| Chain | Residue | Details |
| A | ARG71 |
| site_id | SWS_FT_FI19 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:34417297, ECO:0007744|PDB:7CUW |
| Chain | Residue | Details |
| A | ASP75 |
| site_id | SWS_FT_FI20 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:34417297, ECO:0007744|PDB:7CUB, ECO:0007744|PDB:7CUW, ECO:0007744|PDB:7N9Z |
| Chain | Residue | Details |
| A | HIS98 |
| site_id | SWS_FT_FI21 |
| Number of Residues | 3 |
| Details | BINDING: axial binding residue => ECO:0000269|PubMed:11017202, ECO:0000269|PubMed:33408407, ECO:0007744|PDB:1FFT, ECO:0007744|PDB:6WTI |
| Chain | Residue | Details |
| A | HIS421 | |
| A | HIS419 | |
| A | HIS106 |
| site_id | SWS_FT_FI22 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:11017202, ECO:0007744|PDB:1FFT |
| Chain | Residue | Details |
| A | TRP170 |
| site_id | SWS_FT_FI23 |
| Number of Residues | 5 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:11017202, ECO:0000269|PubMed:33408407, ECO:0007744|PDB:1FFT, ECO:0007744|PDB:6WTI |
| Chain | Residue | Details |
| A | HIS284 | |
| A | HIS333 | |
| A | HIS334 | |
| A | HIS411 | |
| A | ARG482 |
| site_id | SWS_FT_FI24 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:33408407, ECO:0007744|PDB:6WTI |
| Chain | Residue | Details |
| A | TYR288 | |
| A | ARG481 |
| site_id | SWS_FT_FI25 |
| Number of Residues | 2 |
| Details | CROSSLNK: 1'-histidyl-3'-tyrosine (His-Tyr) => ECO:0000269|PubMed:34417297 |
| Chain | Residue | Details |
| A | HIS284 | |
| A | TYR288 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 23 |
| Details | M-CSA 714 |
| Chain | Residue | Details |
| A | TYR61 | proton shuttle (general acid/base) |
| A | ARG71 | electrostatic stabiliser |
| A | ASP75 | electrostatic stabiliser |
| A | MET79 | electron shuttle |
| A | PHE103 | electron shuttle |
| A | ASN124 | proton shuttle (general acid/base) |
| A | ASP135 | proton shuttle (general acid/base) |
| A | ASN142 | proton shuttle (general acid/base) |
| A | SER145 | proton shuttle (general acid/base) |
| A | THR149 | proton shuttle (general acid/base) |
| A | THR201 | proton shuttle (general acid/base) |
| A | THR204 | proton shuttle (general acid/base) |
| A | THR211 | proton shuttle (general acid/base) |
| A | HIS284 | metal ligand, modifies pKa |
| A | GLU286 | proton shuttle (general acid/base) |
| A | TYR288 | electron shuttle, proton shuttle (general acid/base) |
| A | SER299 | proton shuttle (general acid/base) |
| A | SER315 | proton shuttle (general acid/base) |
| A | THR359 | proton shuttle (general acid/base) |
| A | LYS362 | proton shuttle (general acid/base) |
| A | HIS419 | electron shuttle |
| A | PHE420 | electron shuttle |
| A | HIS421 | electron shuttle |
