Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7C5R

Crystal Structure of C150S mutant Glyceraldehyde-3-phosphate dehydrogenase1 from Escherichia coli complexed with BPG at 2.31 Angstrom resolution

Functional Information from GO Data

Chain	GOid	namespace	contents
O	0000166	molecular_function	nucleotide binding
O	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
O	0005737	cellular_component	cytoplasm
O	0006006	biological_process	glucose metabolic process
O	0016491	molecular_function	oxidoreductase activity
O	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
O	0042802	molecular_function	identical protein binding
O	0050661	molecular_function	NADP binding
O	0051287	molecular_function	NAD binding
P	0000166	molecular_function	nucleotide binding
P	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
P	0005737	cellular_component	cytoplasm
P	0006006	biological_process	glucose metabolic process
P	0016491	molecular_function	oxidoreductase activity
P	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
P	0042802	molecular_function	identical protein binding
P	0050661	molecular_function	NADP binding
P	0051287	molecular_function	NAD binding
Q	0000166	molecular_function	nucleotide binding
Q	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
Q	0005737	cellular_component	cytoplasm
Q	0006006	biological_process	glucose metabolic process
Q	0016491	molecular_function	oxidoreductase activity
Q	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
Q	0042802	molecular_function	identical protein binding
Q	0050661	molecular_function	NADP binding
Q	0051287	molecular_function	NAD binding
R	0000166	molecular_function	nucleotide binding
R	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
R	0005737	cellular_component	cytoplasm
R	0006006	biological_process	glucose metabolic process
R	0016491	molecular_function	oxidoreductase activity
R	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
R	0042802	molecular_function	identical protein binding
R	0050661	molecular_function	NADP binding
R	0051287	molecular_function	NAD binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	33
Details	binding site for residue NAD O 401

Chain	Residue
O	ASN7
O	LYS78
O	CYS96
O	THR97
O	GLY98
O	PHE99
O	SER120
O	ALA121
O	THR180
O	ASN315
O	PHE319
O	GLY8
O	DG4402
O	HOH508
O	HOH517
O	HOH520
O	HOH529
O	HOH542
O	HOH551
O	HOH555
O	HOH576
O	HOH578
O	PHE9
O	HOH582
O	HOH639
O	HOH648
O	HOH664
O	GLY10
O	ARG11
O	ILE12
O	ASN33
O	ASP34
O	LEU35

site_id	AC2
Number of Residues	14
Details	binding site for residue DG4 O 402

Chain	Residue
O	SER149
O	SER150
O	THR151
O	HIS177
O	THR180
O	THR182
O	THR209
O	ARG232
O	NAD401
O	CL408
O	HOH517
O	HOH520
O	HOH597
O	HOH700

site_id	AC3
Number of Residues	6
Details	binding site for residue PO4 O 403

Chain	Residue
O	HIS163
O	GLY167
O	ILE168
O	GLU169
O	LEU222
O	LYS225

site_id	AC4
Number of Residues	9
Details	binding site for residue PO4 O 404

Chain	Residue
O	ALA201
O	HOH567
O	HOH598
P	ALA201
Q	ALA201
Q	HOH637
R	ALA201
R	HOH568
R	HOH606

site_id	AC5
Number of Residues	6
Details	binding site for residue EDO O 405

Chain	Residue
O	ARG19
O	HIS286
O	ARG325
O	HOH572
O	HOH624
O	HOH677

site_id	AC6
Number of Residues	3
Details	binding site for residue CL O 406

Chain	Residue
O	LYS227
O	GLY228
O	HOH757

site_id	AC7
Number of Residues	4
Details	binding site for residue CL O 407

Chain	Residue
O	ALA80
O	LYS81
O	ALA108
O	HOH560

site_id	AC8
Number of Residues	5
Details	binding site for residue CL O 408

Chain	Residue
O	HIS177
O	HIS207
O	ALA230
O	ARG232
O	DG4402

site_id	AC9
Number of Residues	32
Details	binding site for residue NAD P 401

Chain	Residue
P	G3H402
P	HOH509
P	HOH510
P	HOH515
P	HOH528
P	HOH548
P	HOH554
P	HOH566
P	HOH591
P	HOH601
P	HOH611
P	HOH620
P	HOH662
P	ASN7
P	GLY8
P	PHE9
P	GLY10
P	ARG11
P	ILE12
P	ASN33
P	ASP34
P	LYS78
P	CYS96
P	THR97
P	GLY98
P	PHE99
P	SER120
P	ALA121
P	SER150
P	THR180
P	ASN315
P	PHE319

site_id	AD1
Number of Residues	11
Details	binding site for residue G3H P 402

Chain	Residue
P	SER149
P	SER150
P	THR151
P	HIS177
P	THR180
P	THR182
P	ARG232
P	NAD401
P	HOH516
P	HOH566
P	HOH580

site_id	AD2
Number of Residues	5
Details	binding site for residue PO4 P 403

Chain	Residue
P	HIS163
P	GLY167
P	ILE168
P	LEU222
P	LYS225

site_id	AD3
Number of Residues	5
Details	binding site for residue EDO P 404

Chain	Residue
P	ARG19
P	HIS286
P	ARG325
P	HOH508
P	HOH582

site_id	AD4
Number of Residues	3
Details	binding site for residue CL P 405

Chain	Residue
O	HOH540
P	LYS227
P	GLY228

site_id	AD5
Number of Residues	35
Details	binding site for residue NAD Q 401

Chain	Residue
Q	ASN7
Q	GLY8
Q	PHE9
Q	GLY10
Q	ARG11
Q	ILE12
Q	ASN33
Q	ASP34
Q	LEU35
Q	LYS78
Q	CYS96
Q	THR97
Q	GLY98
Q	PHE99
Q	SER120
Q	ALA121
Q	SER150
Q	THR180
Q	ASN315
Q	PHE319
Q	G3H402
Q	HOH501
Q	HOH505
Q	HOH506
Q	HOH510
Q	HOH517
Q	HOH522
Q	HOH542
Q	HOH548
Q	HOH564
Q	HOH576
Q	HOH612
Q	HOH621
Q	HOH643
Q	HOH644

site_id	AD6
Number of Residues	12
Details	binding site for residue G3H Q 402

Chain	Residue
Q	SER149
Q	SER150
Q	THR151
Q	HIS177
Q	THR180
Q	THR182
Q	ARG232
Q	NAD401
Q	HOH501
Q	HOH505
Q	HOH519
Q	HOH639

site_id	AD7
Number of Residues	6
Details	binding site for residue PO4 Q 403

Chain	Residue
Q	HIS163
Q	GLY167
Q	ILE168
Q	GLU221
Q	LEU222
Q	LYS225

site_id	AD8
Number of Residues	5
Details	binding site for residue EDO Q 404

Chain	Residue
Q	ARG19
Q	HIS286
Q	ARG325
Q	HOH592
Q	HOH666

site_id	AD9
Number of Residues	3
Details	binding site for residue CL Q 405

Chain	Residue
Q	ALA80
Q	LYS81
Q	ALA108

site_id	AE1
Number of Residues	3
Details	binding site for residue CL Q 406

Chain	Residue
Q	LYS227
Q	GLY228
Q	HOH683

site_id	AE2
Number of Residues	4
Details	binding site for residue CL Q 407

Chain	Residue
Q	HIS177
Q	ALA230
Q	ARG232
Q	HOH519

site_id	AE3
Number of Residues	28
Details	binding site for residue NAD R 401

Chain	Residue
R	ASN7
R	GLY8
R	GLY10
R	ARG11
R	ILE12
R	ASN33
R	ASP34
R	LEU35
R	LYS78
R	CYS96
R	THR97
R	GLY98
R	PHE99
R	SER120
R	ALA121
R	SER150
R	ASN315
R	PHE319
R	G3H402
R	HOH504
R	HOH518
R	HOH538
R	HOH556
R	HOH561
R	HOH574
R	HOH589
R	HOH604
R	HOH613

site_id	AE4
Number of Residues	9
Details	binding site for residue G3H R 402

Chain	Residue
R	THR180
R	GLY181
R	THR182
R	ARG232
R	NAD401
R	HOH501
R	HOH589
R	HOH611
R	HOH623

site_id	AE5
Number of Residues	5
Details	binding site for residue PO4 R 403

Chain	Residue
R	HIS163
R	GLY167
R	ILE168
R	GLU221
R	LYS225

site_id	AE6
Number of Residues	4
Details	binding site for residue EDO R 404

Chain	Residue
O	ASP139
O	GLY140
Q	LYS3
R	ASP63

site_id	AE7
Number of Residues	4
Details	binding site for residue EDO R 405

Chain	Residue
R	ARG19
R	HIS286
R	ARG325
R	HOH523

site_id	AE8
Number of Residues	4
Details	binding site for residue CL R 406

Chain	Residue
Q	HOH554
R	LYS227
R	GLY228
R	HOH635

Functional Information from PROSITE/UniProt

site_id	PS00430
Number of Residues	89
Details	TONB_DEPENDENT_REC_1 TonB-dependent receptor (TBDR) proteins signature 1. hhhhhhssglvprgshmasmskvgingfgrigrlvlrrllevksnidvvaindltspkilayllkhdsnygpfpwsvdfte..................................DSLIVDGK

Chain	Residue	Details
O	HIS-18-LYS70

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)