7C5P

Crystal Structure Glyceraldehyde-3-phosphate-dehydrogenase1 from Escherichia coli complexed with G3P at 2.35 Angstrom resolution.

Functional Information from GO Data

Chain	GOid	namespace	contents
O	0006006	biological_process	glucose metabolic process
O	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
O	0050661	molecular_function	NADP binding
O	0051287	molecular_function	NAD binding
P	0006006	biological_process	glucose metabolic process
P	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
P	0050661	molecular_function	NADP binding
P	0051287	molecular_function	NAD binding
Q	0006006	biological_process	glucose metabolic process
Q	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
Q	0050661	molecular_function	NADP binding
Q	0051287	molecular_function	NAD binding
R	0006006	biological_process	glucose metabolic process
R	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
R	0050661	molecular_function	NADP binding
R	0051287	molecular_function	NAD binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	31
Details	binding site for residue NAD O 401

Chain	Residue
O	ASN7
O	LYS78
O	CYS96
O	THR97
O	GLY98
O	PHE99
O	SER120
O	ALA121
O	CYS150
O	ASN315
O	PHE319
O	GLY8
O	G3H402
O	HOH509
O	HOH511
O	HOH512
O	HOH531
O	HOH544
O	HOH553
O	HOH559
O	HOH584
O	HOH605
O	PHE9
O	HOH610
O	HOH631
O	GLY10
O	ARG11
O	ILE12
O	ASN33
O	ASP34
O	LEU35

---

site_id	AC2
Number of Residues	6
Details	binding site for residue G3H O 402

Chain	Residue
O	THR180
O	GLY181
O	THR182
O	ARG232
O	NAD401
O	HOH591

---

site_id	AC3
Number of Residues	6
Details	binding site for residue PO4 O 403

Chain	Residue
O	HIS163
O	GLY167
O	ILE168
O	GLU169
O	LEU222
O	LYS225

---

site_id	AC4
Number of Residues	7
Details	binding site for residue PO4 O 404

Chain	Residue
O	ALA201
O	GLU202
P	HOH620
Q	ALA201
Q	GLU202
R	PRO234
R	HOH550

---

site_id	AC5
Number of Residues	3
Details	binding site for residue CL O 405

Chain	Residue
O	LYS227
O	GLY228
O	HOH663

---

site_id	AC6
Number of Residues	5
Details	binding site for residue CL O 406

Chain	Residue
O	HIS177
O	HIS207
O	ALA230
O	ARG232
O	HOH547

---

site_id	AC7
Number of Residues	31
Details	binding site for residue NAD P 401

Chain	Residue
P	ASN7
P	GLY8
P	PHE9
P	GLY10
P	ARG11
P	ILE12
P	ASN33
P	ASP34
P	LEU35
P	LYS78
P	CYS96
P	THR97
P	GLY98
P	PHE99
P	SER120
P	ALA121
P	CYS150
P	THR180
P	ASN315
P	PHE319
P	G3H402
P	HOH501
P	HOH508
P	HOH513
P	HOH514
P	HOH531
P	HOH534
P	HOH568
P	HOH595
P	HOH605
Q	HOH511

---

site_id	AC8
Number of Residues	7
Details	binding site for residue G3H P 402

Chain	Residue
P	THR180
P	GLY181
P	THR182
P	ARG232
P	NAD401
P	HOH514
P	HOH545

---

site_id	AC9
Number of Residues	5
Details	binding site for residue PO4 P 403

Chain	Residue
P	LEU222
P	LYS225
P	HIS163
P	GLY167
P	ILE168

---

site_id	AD1
Number of Residues	5
Details	binding site for residue CL P 404

Chain	Residue
O	HOH502
P	LYS227
P	GLY228
P	HOH562
P	HOH653

---

site_id	AD2
Number of Residues	4
Details	binding site for residue CL P 405

Chain	Residue
P	HIS177
P	ALA230
P	ARG232
P	HOH519

---

site_id	AD3
Number of Residues	30
Details	binding site for residue NAD Q 401

Chain	Residue
Q	ASN7
Q	GLY8
Q	PHE9
Q	GLY10
Q	ARG11
Q	ILE12
Q	ASN33
Q	ASP34
Q	LEU35
Q	LYS78
Q	CYS96
Q	THR97
Q	GLY98
Q	PHE99
Q	SER120
Q	ALA121
Q	CYS150
Q	THR180
Q	ASN315
Q	PHE319
Q	G3H402
Q	HOH501
Q	HOH506
Q	HOH515
Q	HOH522
Q	HOH537
Q	HOH538
Q	HOH544
Q	HOH574
Q	HOH617

---

site_id	AD4
Number of Residues	8
Details	binding site for residue G3H Q 402

Chain	Residue
Q	THR180
Q	GLY181
Q	THR182
Q	ARG232
Q	NAD401
Q	HOH506
Q	HOH520
Q	HOH594

---

site_id	AD5
Number of Residues	5
Details	binding site for residue PO4 Q 403

Chain	Residue
Q	HIS163
Q	GLY167
Q	ILE168
Q	LEU222
Q	LYS225

---

site_id	AD6
Number of Residues	4
Details	binding site for residue CL Q 404

Chain	Residue
Q	LYS227
Q	GLY228
Q	HOH631
R	HOH518

---

site_id	AD7
Number of Residues	27
Details	binding site for residue NAD R 401

Chain	Residue
R	GLY8
R	PHE9
R	GLY10
R	ARG11
R	ILE12
R	ASN33
R	ASP34
R	LEU35
R	LYS78
R	CYS96
R	THR97
R	GLY98
R	PHE99
R	SER120
R	ALA121
R	CYS150
R	ASN315
R	PHE319
R	G3H402
R	HOH501
R	HOH505
R	HOH524
R	HOH526
R	HOH536
R	HOH538
R	HOH568
R	HOH572

---

site_id	AD8
Number of Residues	5
Details	binding site for residue G3H R 402

Chain	Residue
R	THR180
R	GLY181
R	THR182
R	ARG232
R	NAD401

---

site_id	AD9
Number of Residues	5
Details	binding site for residue PO4 R 403

Chain	Residue
R	HIS163
R	GLY167
R	ILE168
R	GLU221
R	LYS225

---

Functional Information from PROSITE/UniProt

site_id	PS00071
Number of Residues	8
Details	GAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL

Chain	Residue	Details
O	ALA148-LEU155

---

site_id	PS00430
Number of Residues	89
Details	TONB_DEPENDENT_REC_1 TonB-dependent receptor (TBDR) proteins signature 1. hhhhhhssglvprgshmasmskvgingfgrigrlvlrrllevksnidvvaindltspkilayllkhdsnygpfpwsvdfte..................................DSLIVDGK

Chain	Residue	Details
O	HIS-18-LYS70

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