7BUM

mcGAS bound with pGpA

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0002218	biological_process	activation of innate immune response
A	0002221	biological_process	pattern recognition receptor signaling pathway
A	0002230	biological_process	positive regulation of defense response to virus by host
A	0002376	biological_process	immune system process
A	0002637	biological_process	regulation of immunoglobulin production
A	0002753	biological_process	cytoplasmic pattern recognition receptor signaling pathway
A	0003677	molecular_function	DNA binding
A	0003682	molecular_function	chromatin binding
A	0003690	molecular_function	double-stranded DNA binding
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005525	molecular_function	GTP binding
A	0005546	molecular_function	phosphatidylinositol-4,5-bisphosphate binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005694	cellular_component	chromosome
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006281	biological_process	DNA repair
A	0006974	biological_process	DNA damage response
A	0008289	molecular_function	lipid binding
A	0008340	biological_process	determination of adult lifespan
A	0016604	cellular_component	nuclear body
A	0016740	molecular_function	transferase activity
A	0016779	molecular_function	nucleotidyltransferase activity
A	0031491	molecular_function	nucleosome binding
A	0032479	biological_process	regulation of type I interferon production
A	0032481	biological_process	positive regulation of type I interferon production
A	0035861	cellular_component	site of double-strand break
A	0038001	biological_process	paracrine signaling
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity
A	0045087	biological_process	innate immune response
A	0045738	biological_process	negative regulation of DNA repair
A	0046872	molecular_function	metal ion binding
A	0050776	biological_process	regulation of immune response
A	0050863	biological_process	regulation of T cell activation
A	0051607	biological_process	defense response to virus
A	0061501	molecular_function	2',3'-cyclic GMP-AMP synthase activity
A	0071360	biological_process	cellular response to exogenous dsRNA
A	0140693	molecular_function	molecular condensate scaffold activity
A	0140896	biological_process	cGAS/STING signaling pathway
A	0160004	molecular_function	poly-ADP-D-ribose modification-dependent protein binding
A	0160049	biological_process	negative regulation of cGAS/STING signaling pathway
A	2000042	biological_process	negative regulation of double-strand break repair via homologous recombination
A	2000774	biological_process	positive regulation of cellular senescence
B	0000166	molecular_function	nucleotide binding
B	0002218	biological_process	activation of innate immune response
B	0002221	biological_process	pattern recognition receptor signaling pathway
B	0002230	biological_process	positive regulation of defense response to virus by host
B	0002376	biological_process	immune system process
B	0002637	biological_process	regulation of immunoglobulin production
B	0002753	biological_process	cytoplasmic pattern recognition receptor signaling pathway
B	0003677	molecular_function	DNA binding
B	0003682	molecular_function	chromatin binding
B	0003690	molecular_function	double-stranded DNA binding
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005525	molecular_function	GTP binding
B	0005546	molecular_function	phosphatidylinositol-4,5-bisphosphate binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005694	cellular_component	chromosome
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006281	biological_process	DNA repair
B	0006974	biological_process	DNA damage response
B	0008289	molecular_function	lipid binding
B	0008340	biological_process	determination of adult lifespan
B	0016604	cellular_component	nuclear body
B	0016740	molecular_function	transferase activity
B	0016779	molecular_function	nucleotidyltransferase activity
B	0031491	molecular_function	nucleosome binding
B	0032479	biological_process	regulation of type I interferon production
B	0032481	biological_process	positive regulation of type I interferon production
B	0035861	cellular_component	site of double-strand break
B	0038001	biological_process	paracrine signaling
B	0042802	molecular_function	identical protein binding
B	0042803	molecular_function	protein homodimerization activity
B	0045087	biological_process	innate immune response
B	0045738	biological_process	negative regulation of DNA repair
B	0046872	molecular_function	metal ion binding
B	0050776	biological_process	regulation of immune response
B	0050863	biological_process	regulation of T cell activation
B	0051607	biological_process	defense response to virus
B	0061501	molecular_function	2',3'-cyclic GMP-AMP synthase activity
B	0071360	biological_process	cellular response to exogenous dsRNA
B	0140693	molecular_function	molecular condensate scaffold activity
B	0140896	biological_process	cGAS/STING signaling pathway
B	0160004	molecular_function	poly-ADP-D-ribose modification-dependent protein binding
B	0160049	biological_process	negative regulation of cGAS/STING signaling pathway
B	2000042	biological_process	negative regulation of double-strand break repair via homologous recombination
B	2000774	biological_process	positive regulation of cellular senescence

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue ZN A 601

Chain	Residue
A	HIS378
A	CYS384
A	CYS385
A	CYS392

---

site_id	AC2
Number of Residues	4
Details	binding site for residue ZN B 601

Chain	Residue
B	HIS378
B	CYS384
B	CYS385
B	CYS392

---

site_id	AC3
Number of Residues	9
Details	binding site for Di-nucleotide A A 602 and 5GP A 603

Chain	Residue
A	ASP213
A	SER291
A	ILE309
A	LYS350
A	ARG364
A	SER420
A	TYR421
A	HOH710
A	ILE206

---

site_id	AC4
Number of Residues	9
Details	binding site for Di-nucleotide A B 602 and 5GP B 603

Chain	Residue
B	GLY290
B	SER291
B	PRO292
B	ASP307
B	LYS350
B	ARG364
B	CYS419
B	SER420
B	TYR421

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	132
Details	Region: {"description":"DNA-binding","evidences":[{"source":"PubMed","id":"23647843","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28902841","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28963528","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5N6I","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	82
Details	Region: {"description":"Interaction with collided ribosomes","evidences":[{"source":"UniProtKB","id":"Q8N884","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	10
Details	Motif: {"description":"Nuclear export signal","evidences":[{"source":"UniProtKB","id":"Q8N884","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	20
Details	Motif: {"description":"Nuclear localization signal","evidences":[{"source":"UniProtKB","id":"Q8N884","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	36
Details	Binding site: {"evidences":[{"source":"PubMed","id":"23647843","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"24332030","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4LEZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"23647843","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24332030","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24462292","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28902841","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28963528","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32814054","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32911481","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32913000","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5N6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XZB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XZE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XZG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6X59","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BUJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BUM","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BUQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7JO9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7JOA","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"23647843","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24332030","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24462292","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28902841","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28963528","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32814054","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32911481","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32913000","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5N6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XZB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XZE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XZG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6XJD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BUJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BUM","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BUQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7JO9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7JOA","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"23647843","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24332030","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24462292","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28902841","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28963528","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32814054","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32911480","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32911481","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32913000","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5N6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XZB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XZE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XZG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6X5A","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BUJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BUM","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BUQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7JO9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7JOA","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	Site: {"description":"Arginine-anchor","evidences":[{"source":"PubMed","id":"31808743","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32911480","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32911481","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32913000","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	Site: {"description":"Cleavage; by CASP3","evidences":[{"source":"UniProtKB","id":"Q8N884","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	2
Details	Modified residue: {"description":"N6-lactoyllysine","evidences":[{"source":"PubMed","id":"39322678","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	2
Details	Modified residue: {"description":"PolyADP-ribosyl glutamic acid","evidences":[{"source":"PubMed","id":"35460603","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"Q8N884","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	2
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"Q8N884","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	2
Details	Modified residue: {"description":"5-glutamyl polyglutamate","evidences":[{"source":"PubMed","id":"26829768","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine; by CDK1 and PKB","evidences":[{"source":"PubMed","id":"26440888","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32351706","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	2
Details	Modified residue: {"description":"5-glutamyl glutamate","evidences":[{"source":"PubMed","id":"26829768","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI19
Number of Residues	6
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"Q8N884","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI20
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"32474700","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI21
Number of Residues	2
Details	Modified residue: {"description":"N6-methyllysine","evidences":[{"source":"PubMed","id":"35210392","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI22
Number of Residues	6
Details	Lipidation: {"description":"S-palmitoyl cysteine","evidences":[{"source":"UniProtKB","id":"Q8N884","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI23
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)","evidences":[{"source":"PubMed","id":"27637147","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI24
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate","evidences":[{"source":"PubMed","id":"27637147","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32457395","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI25
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"27637147","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32457395","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI26
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate","evidences":[{"source":"PubMed","id":"29426904","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI27
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate","evidences":[{"source":"UniProtKB","id":"Q8N884","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI28
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)","evidences":[{"source":"PubMed","id":"28095500","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI29
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"UniProtKB","id":"Q8N884","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI30
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"38418882","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---