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7AFT

Cryo-EM structure of the signal sequence-engaged post-translational Sec translocon

Functional Information from GO Data
ChainGOidnamespacecontents
A0005048molecular_functionsignal sequence binding
A0005515molecular_functionprotein binding
A0005783cellular_componentendoplasmic reticulum
A0005784cellular_componentSec61 translocon complex
A0005789cellular_componentendoplasmic reticulum membrane
A0006616biological_processSRP-dependent cotranslational protein targeting to membrane, translocation
A0008320molecular_functionprotein transmembrane transporter activity
A0015031biological_processprotein transport
A0015450molecular_functionprotein-transporting ATPase activity
A0016020cellular_componentmembrane
A0030867cellular_componentrough endoplasmic reticulum membrane
A0030970biological_processretrograde protein transport, ER to cytosol
A0031204biological_processpost-translational protein targeting to membrane, translocation
A0036503biological_processERAD pathway
A0042886biological_processamide transport
A0043022molecular_functionribosome binding
A0044743biological_processprotein transmembrane import into intracellular organelle
A0070843biological_processmisfolded protein transport
A0071256cellular_componenttranslocon complex
A1904680molecular_functionpeptide transmembrane transporter activity
B0005085molecular_functionguanyl-nucleotide exchange factor activity
B0005515molecular_functionprotein binding
B0005783cellular_componentendoplasmic reticulum
B0005784cellular_componentSec61 translocon complex
B0005789cellular_componentendoplasmic reticulum membrane
B0005829cellular_componentcytosol
B0006616biological_processSRP-dependent cotranslational protein targeting to membrane, translocation
B0006886biological_processintracellular protein transport
B0008320molecular_functionprotein transmembrane transporter activity
B0015031biological_processprotein transport
B0015450molecular_functionprotein-transporting ATPase activity
B0016020cellular_componentmembrane
B0030867cellular_componentrough endoplasmic reticulum membrane
B0031204biological_processpost-translational protein targeting to membrane, translocation
B0071256cellular_componenttranslocon complex
C0005198molecular_functionstructural molecule activity
C0005515molecular_functionprotein binding
C0005637cellular_componentnuclear inner membrane
C0005783cellular_componentendoplasmic reticulum
C0005784cellular_componentSec61 translocon complex
C0005789cellular_componentendoplasmic reticulum membrane
C0006605biological_processprotein targeting
C0006614biological_processSRP-dependent cotranslational protein targeting to membrane
C0006616biological_processSRP-dependent cotranslational protein targeting to membrane, translocation
C0006886biological_processintracellular protein transport
C0008320molecular_functionprotein transmembrane transporter activity
C0015031biological_processprotein transport
C0015450molecular_functionprotein-transporting ATPase activity
C0016020cellular_componentmembrane
C0030867cellular_componentrough endoplasmic reticulum membrane
C0031204biological_processpost-translational protein targeting to membrane, translocation
C0071256cellular_componenttranslocon complex
C0071261cellular_componentSsh1 translocon complex
D0003723molecular_functionRNA binding
D0005515molecular_functionprotein binding
D0005634cellular_componentnucleus
D0005637cellular_componentnuclear inner membrane
D0005739cellular_componentmitochondrion
D0005783cellular_componentendoplasmic reticulum
D0005789cellular_componentendoplasmic reticulum membrane
D0006614biological_processSRP-dependent cotranslational protein targeting to membrane
D0006620biological_processpost-translational protein targeting to endoplasmic reticulum membrane
D0008320molecular_functionprotein transmembrane transporter activity
D0015031biological_processprotein transport
D0016020cellular_componentmembrane
D0030867cellular_componentrough endoplasmic reticulum membrane
D0031204biological_processpost-translational protein targeting to membrane, translocation
D0031207cellular_componentSec62/Sec63 complex
D0031965cellular_componentnuclear membrane
D0046967biological_processcytosol to endoplasmic reticulum transport
D0071256cellular_componenttranslocon complex
E0005515molecular_functionprotein binding
E0005783cellular_componentendoplasmic reticulum
E0005789cellular_componentendoplasmic reticulum membrane
E0008320molecular_functionprotein transmembrane transporter activity
E0015031biological_processprotein transport
E0016020cellular_componentmembrane
E0030447biological_processfilamentous growth
E0030867cellular_componentrough endoplasmic reticulum membrane
E0031204biological_processpost-translational protein targeting to membrane, translocation
E0031207cellular_componentSec62/Sec63 complex
E0071256cellular_componenttranslocon complex
E0071944cellular_componentcell periphery
F0005515molecular_functionprotein binding
F0005737cellular_componentcytoplasm
F0005783cellular_componentendoplasmic reticulum
F0008320molecular_functionprotein transmembrane transporter activity
F0015031biological_processprotein transport
F0030867cellular_componentrough endoplasmic reticulum membrane
F0031204biological_processpost-translational protein targeting to membrane, translocation
F0031207cellular_componentSec62/Sec63 complex
F0071256cellular_componenttranslocon complex
G0005515molecular_functionprotein binding
G0005783cellular_componentendoplasmic reticulum
G0005789cellular_componentendoplasmic reticulum membrane
G0008320molecular_functionprotein transmembrane transporter activity
G0015031biological_processprotein transport
G0016020cellular_componentmembrane
G0030867cellular_componentrough endoplasmic reticulum membrane
G0031204biological_processpost-translational protein targeting to membrane, translocation
G0031207cellular_componentSec62/Sec63 complex
G0071256cellular_componenttranslocon complex
H0000750biological_processpheromone-dependent signal transduction involved in conjugation with cellular fusion
H0000772molecular_functionmating pheromone activity
H0005576cellular_componentextracellular region
H0007618biological_processmating
H0019953biological_processsexual reproduction
Functional Information from PROSITE/UniProt
site_idPS00755
Number of Residues20
DetailsSECY_1 Protein secY signature 1. TLLeLGVsPIItSSMIFQFL
ChainResidueDetails
ATHR76-LEU95

site_idPS00756
Number of Residues19
DetailsSECY_2 Protein secY signature 2. LLdElLSkgyGLGSGiSLF
ChainResidueDetails
ALEU166-PHE184

site_idPS00636
Number of Residues20
DetailsDNAJ_1 Nt-dnaJ domain signature. YVqITkaYEsLtDelvRQNY
ChainResidueDetails
DTYR173-TYR192

site_idPS01067
Number of Residues29
DetailsSECE_SEC61G Protein secE/sec61-gamma signature. FvREgTqfLaKckKPdlkEytkivKAVGI
ChainResidueDetails
CPHE28-ILE56

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues216
DetailsTOPO_DOM: Cytoplasmic => ECO:0000255
ChainResidueDetails
GSER-123-ASP24
GSER80-ASN149
AASP168-GLU212
AGLN261-ASN290
AGLU382-LYS416
AGLU460-MET480

site_idSWS_FT_FI2
Number of Residues39
DetailsTRANSMEM: Helical => ECO:0000255
ChainResidueDetails
GTYR25-TRP44
GALA59-LEU79
DPRO221-VAL239

site_idSWS_FT_FI3
Number of Residues13
DetailsTOPO_DOM: Lumenal => ECO:0000255
ChainResidueDetails
GPRO45-GLY58
DSER240-GLU663
AARG225-ASN240
AGLN312-LYS361
AGLY435-ASP437

site_idSWS_FT_FI4
Number of Residues1
DetailsMOD_RES: N-acetylserine => ECO:0007744|PubMed:22814378
ChainResidueDetails
GSER-123
EASN12

site_idSWS_FT_FI5
Number of Residues21
DetailsTRANSMEM: Helical; Name=3 => ECO:0000305
ChainResidueDetails
AVAL120-ALA141

site_idSWS_FT_FI6
Number of Residues20
DetailsTRANSMEM: Helical; Name=4 => ECO:0000305
ChainResidueDetails
ALEU147-LEU167

site_idSWS_FT_FI7
Number of Residues11
DetailsTRANSMEM: Helical; Name=5 => ECO:0000305
ChainResidueDetails
AGLY213-VAL224

site_idSWS_FT_FI8
Number of Residues19
DetailsTRANSMEM: Helical; Name=6 => ECO:0000305
ChainResidueDetails
ALEU241-LEU260

site_idSWS_FT_FI9
Number of Residues20
DetailsTRANSMEM: Helical; Name=7 => ECO:0000305
ChainResidueDetails
ATHR291-PHE311

site_idSWS_FT_FI10
Number of Residues19
DetailsTRANSMEM: Helical; Name=8 => ECO:0000305
ChainResidueDetails
ATHR362-ILE381

site_idSWS_FT_FI11
Number of Residues17
DetailsTRANSMEM: Helical; Name=9 => ECO:0000305
ChainResidueDetails
AILE417-VAL434

site_idSWS_FT_FI12
Number of Residues21
DetailsTRANSMEM: Helical; Name=10 => ECO:0000305
ChainResidueDetails
ALEU438-TYR459

218853

PDB entries from 2024-04-24

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