7AAV

Human pre-Bact-2 spliceosome core structure

Functional Information from GO Data

Chain	GOid	namespace	contents
8	0000398	biological_process	mRNA splicing, via spliceosome
8	0003723	molecular_function	RNA binding
8	0003729	molecular_function	mRNA binding
8	0005515	molecular_function	protein binding
8	0005634	cellular_component	nucleus
8	0005654	cellular_component	nucleoplasm
8	0005681	cellular_component	spliceosomal complex
8	0006397	biological_process	mRNA processing
8	0008380	biological_process	RNA splicing
8	0016607	cellular_component	nuclear speck
8	0071007	cellular_component	U2-type catalytic step 2 spliceosome
8	0071013	cellular_component	catalytic step 2 spliceosome
8	1990403	biological_process	embryonic brain development
A	0000244	biological_process	spliceosomal tri-snRNP complex assembly
A	0000375	biological_process	RNA splicing, via transesterification reactions
A	0000398	biological_process	mRNA splicing, via spliceosome
A	0003723	molecular_function	RNA binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005681	cellular_component	spliceosomal complex
A	0005682	cellular_component	U5 snRNP
A	0006397	biological_process	mRNA processing
A	0008233	molecular_function	peptidase activity
A	0008237	molecular_function	metallopeptidase activity
A	0008380	biological_process	RNA splicing
A	0016020	cellular_component	membrane
A	0016607	cellular_component	nuclear speck
A	0017070	molecular_function	U6 snRNA binding
A	0030532	cellular_component	small nuclear ribonucleoprotein complex
A	0030619	molecular_function	U1 snRNA binding
A	0030620	molecular_function	U2 snRNA binding
A	0030623	molecular_function	U5 snRNA binding
A	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
A	0070530	molecular_function	K63-linked polyubiquitin modification-dependent protein binding
A	0071005	cellular_component	U2-type precatalytic spliceosome
A	0071006	cellular_component	U2-type catalytic step 1 spliceosome
A	0071007	cellular_component	U2-type catalytic step 2 spliceosome
A	0071013	cellular_component	catalytic step 2 spliceosome
A	0071222	biological_process	cellular response to lipopolysaccharide
A	0071356	biological_process	cellular response to tumor necrosis factor
A	0097157	molecular_function	pre-mRNA intronic binding
A	0140492	molecular_function	metal-dependent deubiquitinase activity
G	0000398	biological_process	mRNA splicing, via spliceosome
G	0000974	cellular_component	Prp19 complex
G	0001650	cellular_component	fibrillar center
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005662	cellular_component	DNA replication factor A complex
G	0005681	cellular_component	spliceosomal complex
G	0006397	biological_process	mRNA processing
G	0008380	biological_process	RNA splicing
G	0016607	cellular_component	nuclear speck
G	0031965	cellular_component	nuclear membrane
G	0034504	biological_process	protein localization to nucleus
G	0071007	cellular_component	U2-type catalytic step 2 spliceosome
G	0071013	cellular_component	catalytic step 2 spliceosome
G	1900087	biological_process	positive regulation of G1/S transition of mitotic cell cycle
I	0000398	biological_process	mRNA splicing, via spliceosome
I	0003723	molecular_function	RNA binding
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005681	cellular_component	spliceosomal complex
I	0006397	biological_process	mRNA processing
I	0008380	biological_process	RNA splicing
I	0071005	cellular_component	U2-type precatalytic spliceosome
I	0071011	cellular_component	precatalytic spliceosome
K	0000398	biological_process	mRNA splicing, via spliceosome
K	0001527	cellular_component	microfibril
K	0003723	molecular_function	RNA binding
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005681	cellular_component	spliceosomal complex
K	0005684	cellular_component	U2-type spliceosomal complex
K	0005813	cellular_component	centrosome
K	0006397	biological_process	mRNA processing
K	0008380	biological_process	RNA splicing
K	0071005	cellular_component	U2-type precatalytic spliceosome
L	0000077	biological_process	DNA damage checkpoint signaling
L	0000398	biological_process	mRNA splicing, via spliceosome
L	0000974	cellular_component	Prp19 complex
L	0000977	molecular_function	RNA polymerase II transcription regulatory region sequence-specific DNA binding
L	0000981	molecular_function	DNA-binding transcription factor activity, RNA polymerase II-specific
L	0001228	molecular_function	DNA-binding transcription activator activity, RNA polymerase II-specific
L	0003677	molecular_function	DNA binding
L	0003723	molecular_function	RNA binding
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005654	cellular_component	nucleoplasm
L	0005662	cellular_component	DNA replication factor A complex
L	0005681	cellular_component	spliceosomal complex
L	0005737	cellular_component	cytoplasm
L	0006281	biological_process	DNA repair
L	0006357	biological_process	regulation of transcription by RNA polymerase II
L	0006397	biological_process	mRNA processing
L	0008380	biological_process	RNA splicing
L	0016020	cellular_component	membrane
L	0016607	cellular_component	nuclear speck
L	0042802	molecular_function	identical protein binding
L	0045944	biological_process	positive regulation of transcription by RNA polymerase II
L	0071007	cellular_component	U2-type catalytic step 2 spliceosome
L	0071013	cellular_component	catalytic step 2 spliceosome
L	0071987	molecular_function	WD40-repeat domain binding
N	0000398	biological_process	mRNA splicing, via spliceosome
N	0003676	molecular_function	nucleic acid binding
N	0003677	molecular_function	DNA binding
N	0005515	molecular_function	protein binding
N	0005634	cellular_component	nucleus
N	0005654	cellular_component	nucleoplasm
N	0005681	cellular_component	spliceosomal complex
N	0006397	biological_process	mRNA processing
N	0008270	molecular_function	zinc ion binding
N	0008380	biological_process	RNA splicing
N	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
N	0046872	molecular_function	metal ion binding
N	0071005	cellular_component	U2-type precatalytic spliceosome
P	0000398	biological_process	mRNA splicing, via spliceosome
P	0000974	cellular_component	Prp19 complex
P	0003676	molecular_function	nucleic acid binding
P	0003723	molecular_function	RNA binding
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005654	cellular_component	nucleoplasm
P	0005681	cellular_component	spliceosomal complex
P	0005737	cellular_component	cytoplasm
P	0006397	biological_process	mRNA processing
P	0008380	biological_process	RNA splicing
P	0017070	molecular_function	U6 snRNA binding
P	0033120	biological_process	positive regulation of RNA splicing
P	0036002	molecular_function	pre-mRNA binding
P	0042307	biological_process	positive regulation of protein import into nucleus
P	0045292	biological_process	mRNA cis splicing, via spliceosome
P	0046827	biological_process	positive regulation of protein export from nucleus
P	0046872	molecular_function	metal ion binding
P	0048306	molecular_function	calcium-dependent protein binding
P	0071006	cellular_component	U2-type catalytic step 1 spliceosome
P	0071007	cellular_component	U2-type catalytic step 2 spliceosome
P	0071013	cellular_component	catalytic step 2 spliceosome
P	0071466	biological_process	cellular response to xenobiotic stimulus
q	0000398	biological_process	mRNA splicing, via spliceosome
q	0005515	molecular_function	protein binding
q	0005634	cellular_component	nucleus
q	0005654	cellular_component	nucleoplasm
q	0005737	cellular_component	cytoplasm
q	0015030	cellular_component	Cajal body
q	0031386	molecular_function	protein tag activity
q	0036211	biological_process	protein modification process
q	1903955	biological_process	positive regulation of protein targeting to mitochondrion
Q	0000398	biological_process	mRNA splicing, via spliceosome
Q	0000785	cellular_component	chromatin
Q	0005515	molecular_function	protein binding
Q	0005634	cellular_component	nucleus
Q	0005654	cellular_component	nucleoplasm
Q	0005681	cellular_component	spliceosomal complex
Q	0006397	biological_process	mRNA processing
Q	0008380	biological_process	RNA splicing
Q	0016922	molecular_function	nuclear receptor binding
Q	0030374	molecular_function	nuclear receptor coactivator activity
Q	0045893	biological_process	positive regulation of DNA-templated transcription
Q	0071007	cellular_component	U2-type catalytic step 2 spliceosome
Q	2000825	biological_process	positive regulation of androgen receptor activity
r	0000398	biological_process	mRNA splicing, via spliceosome
r	0003723	molecular_function	RNA binding
r	0003924	molecular_function	GTPase activity
r	0005515	molecular_function	protein binding
r	0005525	molecular_function	GTP binding
r	0005634	cellular_component	nucleus
r	0005654	cellular_component	nucleoplasm
r	0005681	cellular_component	spliceosomal complex
r	0005829	cellular_component	cytosol
r	0006397	biological_process	mRNA processing
r	0008380	biological_process	RNA splicing
r	0015030	cellular_component	Cajal body
r	0016020	cellular_component	membrane
r	0016607	cellular_component	nuclear speck
r	0030623	molecular_function	U5 snRNA binding
r	0042220	biological_process	response to cocaine
r	0043231	cellular_component	intracellular membrane-bounded organelle
r	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
r	0071005	cellular_component	U2-type precatalytic spliceosome
r	0071007	cellular_component	U2-type catalytic step 2 spliceosome
r	0071013	cellular_component	catalytic step 2 spliceosome
r	0071466	biological_process	cellular response to xenobiotic stimulus
R	0000398	biological_process	mRNA splicing, via spliceosome
R	0000974	cellular_component	Prp19 complex
R	0003723	molecular_function	RNA binding
R	0005515	molecular_function	protein binding
R	0005634	cellular_component	nucleus
R	0005654	cellular_component	nucleoplasm
R	0005681	cellular_component	spliceosomal complex
R	0005739	cellular_component	mitochondrion
R	0006397	biological_process	mRNA processing
R	0008380	biological_process	RNA splicing
R	0016607	cellular_component	nuclear speck
R	0045292	biological_process	mRNA cis splicing, via spliceosome
R	0071007	cellular_component	U2-type catalytic step 2 spliceosome
R	0071013	cellular_component	catalytic step 2 spliceosome
v	0000122	biological_process	negative regulation of transcription by RNA polymerase II
v	0000398	biological_process	mRNA splicing, via spliceosome
v	0003713	molecular_function	transcription coactivator activity
v	0003714	molecular_function	transcription corepressor activity
v	0003723	molecular_function	RNA binding
v	0005112	molecular_function	Notch binding
v	0005515	molecular_function	protein binding
v	0005634	cellular_component	nucleus
v	0005654	cellular_component	nucleoplasm
v	0005681	cellular_component	spliceosomal complex
v	0006357	biological_process	regulation of transcription by RNA polymerase II
v	0006397	biological_process	mRNA processing
v	0008024	cellular_component	cyclin/CDK positive transcription elongation factor complex
v	0008380	biological_process	RNA splicing
v	0016363	cellular_component	nuclear matrix
v	0016604	cellular_component	nuclear body
v	0016607	cellular_component	nuclear speck
v	0016922	molecular_function	nuclear receptor binding
v	0019899	molecular_function	enzyme binding
v	0030511	biological_process	positive regulation of transforming growth factor beta receptor signaling pathway
v	0042771	biological_process	intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
v	0042809	molecular_function	nuclear vitamin D receptor binding
v	0042974	molecular_function	nuclear retinoic acid receptor binding
v	0043923	biological_process	positive regulation by host of viral transcription
v	0045892	biological_process	negative regulation of DNA-templated transcription
v	0045944	biological_process	positive regulation of transcription by RNA polymerase II
v	0046332	molecular_function	SMAD binding
v	0048026	biological_process	positive regulation of mRNA splicing, via spliceosome
v	0048384	biological_process	retinoic acid receptor signaling pathway
v	0048385	biological_process	regulation of retinoic acid receptor signaling pathway
v	0050681	molecular_function	nuclear androgen receptor binding
v	0050769	biological_process	positive regulation of neurogenesis
v	0070562	biological_process	regulation of vitamin D receptor signaling pathway
v	0070564	biological_process	positive regulation of vitamin D receptor signaling pathway
v	0071007	cellular_component	U2-type catalytic step 2 spliceosome
v	0071013	cellular_component	catalytic step 2 spliceosome
v	0071300	biological_process	cellular response to retinoic acid

Functional Information from PROSITE/UniProt

site_id	PS00028
Number of Residues	23
Details	ZINC_FINGER_C2H2_1 Zinc finger C2H2 type domain signature. Cnv..CdcvVkdsinfldHingkkH

Chain	Residue	Details
N	CYS82-HIS104

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site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. LLSCsmDcKIKLWEV

Chain	Residue	Details
8	LEU304-VAL318
G	LEU303-VAL317
G	ILE345-LEU359

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site_id	PS00997
Number of Residues	23
Details	G10_1 G10 protein signature 1. LCClRCiqtrDtNfgtnCiCRVP

Chain	Residue	Details
Q	LEU100-PRO122

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site_id	PS00998
Number of Residues	10
Details	G10_2 G10 protein signature 2. CtHCGCRGCS

Chain	Residue	Details
Q	CYS134-SER143

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	24
Details	ZN_FING: Matrin-type

Chain	Residue	Details
N	TYR80-HIS104
I	SER226
r	ASN258

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site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: N-acetylalanine => ECO:0007744|PubMed:22814378

Chain	Residue	Details
N	ALA2
v	SER182
v	SER190
v	SER446
v	SER479
v	SER481

---

site_id	SWS_FT_FI3
Number of Residues	9
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
N	LYS8
P	SER102
N	LYS36
N	LYS39
N	LYS55
N	LYS61
N	LYS70
N	LYS102
N	LYS123

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25772364, ECO:0007744|PubMed:28112733

Chain	Residue	Details
N	LYS45
N	LYS64
K	SER432

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site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692

Chain	Residue	Details
v	SER234

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site_id	SWS_FT_FI6
Number of Residues	12
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
v	LYS23
v	LYS416
v	LYS441
v	LYS452
v	LYS115
v	LYS122
v	LYS141
v	LYS158
v	LYS258
v	LYS286
v	LYS339
v	LYS344

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25772364, ECO:0007744|PubMed:28112733

Chain	Residue	Details
K	SER132
v	LYS509

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site_id	SWS_FT_FI8
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25218447, ECO:0007744|PubMed:28112733

Chain	Residue	Details
v	LYS81

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site_id	SWS_FT_FI9
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25772364

Chain	Residue	Details
v	LYS97
L	THR411

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site_id	SWS_FT_FI10
Number of Residues	3
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25218447, ECO:0007744|PubMed:25772364, ECO:0007744|PubMed:28112733

Chain	Residue	Details
v	LYS170
v	LYS193
v	LYS240
K	LYS371
K	LYS381
K	LYS415
K	LYS418

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site_id	SWS_FT_FI11
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25755297, ECO:0007744|PubMed:28112733

Chain	Residue	Details
K	LYS249

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site_id	SWS_FT_FI12
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0000269|PubMed:18583928, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163

Chain	Residue	Details
L	THR424
L	THR438

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site_id	SWS_FT_FI13
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163

Chain	Residue	Details
L	THR430

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site_id	SWS_FT_FI14
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648

Chain	Residue	Details
L	SER437

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site_id	SWS_FT_FI15
Number of Residues	3
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
L	LYS135
L	LYS487

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site_id	SWS_FT_FI16
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25755297, ECO:0007744|PubMed:25772364, ECO:0007744|PubMed:28112733

Chain	Residue	Details
L	LYS219

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