6ZIB

CRYSTAL STRUCTURE OF RAT PEROXISOMAL MULTIFUNCTIONAL ENZYME TYPE-1 (RPMFE1) COMPLEXED WITH ACETOACETYL-COA AND NADH

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
AAA	0003824	molecular_function	catalytic activity
AAA	0003857	molecular_function	(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity
AAA	0004165	molecular_function	delta(3)-delta(2)-enoyl-CoA isomerase activity
AAA	0004300	molecular_function	enoyl-CoA hydratase activity
AAA	0005777	cellular_component	peroxisome
AAA	0005782	cellular_component	peroxisomal matrix
AAA	0005829	cellular_component	cytosol
AAA	0006629	biological_process	lipid metabolic process
AAA	0006631	biological_process	fatty acid metabolic process
AAA	0006633	biological_process	fatty acid biosynthetic process
AAA	0006635	biological_process	fatty acid beta-oxidation
AAA	0016491	molecular_function	oxidoreductase activity
AAA	0016509	molecular_function	long-chain (3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity
AAA	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
AAA	0016829	molecular_function	lyase activity
AAA	0016853	molecular_function	isomerase activity
AAA	0016863	molecular_function	intramolecular oxidoreductase activity, transposing C=C bonds
AAA	0018812	molecular_function	3-hydroxyacyl-CoA dehydratase activity
AAA	0019899	molecular_function	enzyme binding
AAA	0033540	biological_process	fatty acid beta-oxidation using acyl-CoA oxidase
AAA	0070403	molecular_function	NAD+ binding
BBB	0003824	molecular_function	catalytic activity
BBB	0003857	molecular_function	(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity
BBB	0004165	molecular_function	delta(3)-delta(2)-enoyl-CoA isomerase activity
BBB	0004300	molecular_function	enoyl-CoA hydratase activity
BBB	0005777	cellular_component	peroxisome
BBB	0005782	cellular_component	peroxisomal matrix
BBB	0005829	cellular_component	cytosol
BBB	0006629	biological_process	lipid metabolic process
BBB	0006631	biological_process	fatty acid metabolic process
BBB	0006633	biological_process	fatty acid biosynthetic process
BBB	0006635	biological_process	fatty acid beta-oxidation
BBB	0016491	molecular_function	oxidoreductase activity
BBB	0016509	molecular_function	long-chain (3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity
BBB	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
BBB	0016829	molecular_function	lyase activity
BBB	0016853	molecular_function	isomerase activity
BBB	0016863	molecular_function	intramolecular oxidoreductase activity, transposing C=C bonds
BBB	0018812	molecular_function	3-hydroxyacyl-CoA dehydratase activity
BBB	0019899	molecular_function	enzyme binding
BBB	0033540	biological_process	fatty acid beta-oxidation using acyl-CoA oxidase
BBB	0070403	molecular_function	NAD+ binding

Functional Information from PROSITE/UniProt

site_id	PS00067
Number of Residues	25
Details	3HCDH 3-hydroxyacyl-CoA dehydrogenase signature. NcyGFVgNRmlaPYYnqgff.LLeeG

Chain	Residue	Details
AAA	ASN474-GLY498

---

site_id	PS00166
Number of Residues	21
Details	ENOYL_COA_HYDRATASE Enoyl-CoA hydratase/isomerase signature. LAaIQGvalGGGlelaLgCHY

Chain	Residue	Details
AAA	LEU90-TYR110

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	560
Details	Region: {"description":"Enoyl-CoA hydratase / isomerase"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Site: {"description":"Important for catalytic activity","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Modified residue: {"description":"Blocked amino end (Ala)"}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	18
Details	Modified residue: {"description":"N6-succinyllysine","evidences":[{"source":"UniProtKB","id":"Q9DBM2","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	14
Details	Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"UniProtKB","id":"Q9DBM2","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	6
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"Q9DBM2","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"Q08426","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"Q08426","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---