6ZBT

Structure of 14-3-3 gamma in complex with Nedd4-2 14-3-3 binding motif Ser342

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0002842	biological_process	positive regulation of T cell mediated immune response to tumor cell
A	0003723	molecular_function	RNA binding
A	0005080	molecular_function	protein kinase C binding
A	0005159	molecular_function	insulin-like growth factor receptor binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005925	cellular_component	focal adhesion
A	0006469	biological_process	negative regulation of protein kinase activity
A	0006605	biological_process	protein targeting
A	0007165	biological_process	signal transduction
A	0008104	biological_process	protein localization
A	0008426	molecular_function	protein kinase C inhibitor activity
A	0009966	biological_process	regulation of signal transduction
A	0016020	cellular_component	membrane
A	0019904	molecular_function	protein domain specific binding
A	0022409	biological_process	positive regulation of cell-cell adhesion
A	0030971	molecular_function	receptor tyrosine kinase binding
A	0031982	cellular_component	vesicle
A	0032869	biological_process	cellular response to insulin stimulus
A	0032880	biological_process	regulation of protein localization
A	0042149	biological_process	cellular response to glucose starvation
A	0042802	molecular_function	identical protein binding
A	0045202	cellular_component	synapse
A	0045664	biological_process	regulation of neuron differentiation
A	0048167	biological_process	regulation of synaptic plasticity
A	0050870	biological_process	positive regulation of T cell activation
A	0070062	cellular_component	extracellular exosome
A	0098793	cellular_component	presynapse
A	0140031	molecular_function	phosphorylation-dependent protein binding
A	0140311	molecular_function	protein sequestering activity
A	1904262	biological_process	negative regulation of TORC1 signaling
B	0002842	biological_process	positive regulation of T cell mediated immune response to tumor cell
B	0003723	molecular_function	RNA binding
B	0005080	molecular_function	protein kinase C binding
B	0005159	molecular_function	insulin-like growth factor receptor binding
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005925	cellular_component	focal adhesion
B	0006469	biological_process	negative regulation of protein kinase activity
B	0006605	biological_process	protein targeting
B	0007165	biological_process	signal transduction
B	0008104	biological_process	protein localization
B	0008426	molecular_function	protein kinase C inhibitor activity
B	0009966	biological_process	regulation of signal transduction
B	0016020	cellular_component	membrane
B	0019904	molecular_function	protein domain specific binding
B	0022409	biological_process	positive regulation of cell-cell adhesion
B	0030971	molecular_function	receptor tyrosine kinase binding
B	0031982	cellular_component	vesicle
B	0032869	biological_process	cellular response to insulin stimulus
B	0032880	biological_process	regulation of protein localization
B	0042149	biological_process	cellular response to glucose starvation
B	0042802	molecular_function	identical protein binding
B	0045202	cellular_component	synapse
B	0045664	biological_process	regulation of neuron differentiation
B	0048167	biological_process	regulation of synaptic plasticity
B	0050870	biological_process	positive regulation of T cell activation
B	0070062	cellular_component	extracellular exosome
B	0098793	cellular_component	presynapse
B	0140031	molecular_function	phosphorylation-dependent protein binding
B	0140311	molecular_function	protein sequestering activity
B	1904262	biological_process	negative regulation of TORC1 signaling
C	0002842	biological_process	positive regulation of T cell mediated immune response to tumor cell
C	0003723	molecular_function	RNA binding
C	0005080	molecular_function	protein kinase C binding
C	0005159	molecular_function	insulin-like growth factor receptor binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005925	cellular_component	focal adhesion
C	0006469	biological_process	negative regulation of protein kinase activity
C	0006605	biological_process	protein targeting
C	0007165	biological_process	signal transduction
C	0008104	biological_process	protein localization
C	0008426	molecular_function	protein kinase C inhibitor activity
C	0009966	biological_process	regulation of signal transduction
C	0016020	cellular_component	membrane
C	0019904	molecular_function	protein domain specific binding
C	0022409	biological_process	positive regulation of cell-cell adhesion
C	0030971	molecular_function	receptor tyrosine kinase binding
C	0031982	cellular_component	vesicle
C	0032869	biological_process	cellular response to insulin stimulus
C	0032880	biological_process	regulation of protein localization
C	0042149	biological_process	cellular response to glucose starvation
C	0042802	molecular_function	identical protein binding
C	0045202	cellular_component	synapse
C	0045664	biological_process	regulation of neuron differentiation
C	0048167	biological_process	regulation of synaptic plasticity
C	0050870	biological_process	positive regulation of T cell activation
C	0070062	cellular_component	extracellular exosome
C	0098793	cellular_component	presynapse
C	0140031	molecular_function	phosphorylation-dependent protein binding
C	0140311	molecular_function	protein sequestering activity
C	1904262	biological_process	negative regulation of TORC1 signaling
D	0002842	biological_process	positive regulation of T cell mediated immune response to tumor cell
D	0003723	molecular_function	RNA binding
D	0005080	molecular_function	protein kinase C binding
D	0005159	molecular_function	insulin-like growth factor receptor binding
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005925	cellular_component	focal adhesion
D	0006469	biological_process	negative regulation of protein kinase activity
D	0006605	biological_process	protein targeting
D	0007165	biological_process	signal transduction
D	0008104	biological_process	protein localization
D	0008426	molecular_function	protein kinase C inhibitor activity
D	0009966	biological_process	regulation of signal transduction
D	0016020	cellular_component	membrane
D	0019904	molecular_function	protein domain specific binding
D	0022409	biological_process	positive regulation of cell-cell adhesion
D	0030971	molecular_function	receptor tyrosine kinase binding
D	0031982	cellular_component	vesicle
D	0032869	biological_process	cellular response to insulin stimulus
D	0032880	biological_process	regulation of protein localization
D	0042149	biological_process	cellular response to glucose starvation
D	0042802	molecular_function	identical protein binding
D	0045202	cellular_component	synapse
D	0045664	biological_process	regulation of neuron differentiation
D	0048167	biological_process	regulation of synaptic plasticity
D	0050870	biological_process	positive regulation of T cell activation
D	0070062	cellular_component	extracellular exosome
D	0098793	cellular_component	presynapse
D	0140031	molecular_function	phosphorylation-dependent protein binding
D	0140311	molecular_function	protein sequestering activity
D	1904262	biological_process	negative regulation of TORC1 signaling

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	binding site for residue CFH A 301

Chain	Residue
A	PHE201
A	ASP202
A	CFH302
C	PHE201
C	ARG227

---

site_id	AC2
Number of Residues	6
Details	binding site for residue CFH A 302

Chain	Residue
C	PHE201
C	ASP202
A	PHE201
A	ARG227
A	CFH301
C	LYS198

---

site_id	AC3
Number of Residues	7
Details	binding site for residue CFH B 301

Chain	Residue
B	LEU208
B	ASP209
C	ILE66
C	LYS78
D	GLN9
D	ARG12
D	LEU13

---

site_id	AC4
Number of Residues	10
Details	binding site for residue CFH D 301

Chain	Residue
A	ILE66
A	THR70
A	LYS78
A	MET81
B	GLN9
B	ARG12
B	LEU13
D	ILE205
D	LEU208
D	ASP209

---

site_id	AC5
Number of Residues	15
Details	binding site for Di-peptide CYS E 341 and SEP E 342

Chain	Residue
A	LYS50
A	ARG57
A	ARG132
A	TYR133
A	LEU177
A	ASN178
A	VAL181
A	ASN229
E	ARG339
E	SER340
E	VAL343
E	THR344
E	HOH401
E	HOH402
E	HOH403

---

site_id	AC6
Number of Residues	12
Details	binding site for Di-peptide SEP E 342 and VAL E 343

Chain	Residue
A	LYS50
A	ARG57
A	ARG132
A	TYR133
A	GLY174
A	LEU177
A	ASN178
E	CYS341
E	THR344
E	HOH401
E	HOH402
E	HOH403

---

site_id	AC7
Number of Residues	15
Details	binding site for Di-peptide CYS F 341 and SEP F 342

Chain	Residue
C	LYS50
C	ARG57
C	ARG132
C	TYR133
C	LEU177
C	ASN178
C	VAL181
C	ASN229
F	ARG339
F	SER340
F	VAL343
F	THR344
F	HOH402
F	HOH403
F	HOH404

---

site_id	AC8
Number of Residues	12
Details	binding site for Di-peptide SEP F 342 and VAL F 343

Chain	Residue
C	LYS50
C	ARG57
C	ARG132
C	TYR133
C	GLY174
C	LEU177
C	ASN178
F	CYS341
F	THR344
F	HOH402
F	HOH403
F	HOH404

---

site_id	AC9
Number of Residues	14
Details	binding site for Di-peptide CYS G 341 and SEP G 342

Chain	Residue
B	LYS50
B	ARG57
B	ARG132
B	TYR133
B	LEU177
B	ASN178
B	VAL181
B	ASN229
G	SER340
G	VAL343
G	THR344
G	HOH401
G	HOH402
G	HOH403

---

site_id	AD1
Number of Residues	12
Details	binding site for Di-peptide SEP G 342 and VAL G 343

Chain	Residue
B	TYR133
B	GLY174
B	LEU177
B	ASN178
G	CYS341
G	THR344
G	HOH401
G	HOH402
G	HOH403
B	LYS50
B	ARG57
B	ARG132

---

site_id	AD2
Number of Residues	13
Details	binding site for Di-peptide CYS H 341 and SEP H 342

Chain	Residue
D	LYS50
D	ARG57
D	ARG132
D	TYR133
D	LEU177
D	ASN178
D	VAL181
D	ASN229
H	SER340
H	VAL343
H	THR344
H	HOH401
H	HOH402

---

site_id	AD3
Number of Residues	12
Details	binding site for Di-peptide SEP H 342 and VAL H 343

Chain	Residue
D	LYS50
D	ARG57
D	ARG132
D	TYR133
D	GLY174
D	LEU177
D	ASN178
H	SER340
H	CYS341
H	THR344
H	HOH401
H	HOH402

---

Functional Information from PROSITE/UniProt

site_id	PS00796
Number of Residues	11
Details	1433_1 14-3-3 proteins signature 1. RNLLSVAYKNV

Chain	Residue	Details
A	ARG42-VAL52

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	MOD_RES: Phosphoserine; by WNK1 and WNK4 => ECO:0000269|PubMed:15328345, ECO:0000269|PubMed:20525693, ECO:0007744|PubMed:18669648

Chain	Residue	Details
E	SEP342
F	SEP342
G	SEP342
H	SEP342
C	ARG57
C	ARG132
D	ARG57
D	ARG132

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	MOD_RES: N-acetylmethionine; in 14-3-3 protein gamma; alternate; partial => ECO:0000269|Ref.7

Chain	Residue	Details
A	MET1
B	MET1
C	MET1
D	MET1

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: N-acetylvaline; partial => ECO:0000269|PubMed:14534293, ECO:0000269|Ref.7, ECO:0000269|Ref.9, ECO:0007744|PubMed:19413330

Chain	Residue	Details
A	VAL2
B	VAL2
C	VAL2
D	VAL2

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER71
B	SER71
C	SER71
D	SER71

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P61983

Chain	Residue	Details
A	TYR133
B	TYR133
C	TYR133
D	TYR133

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0000269|Ref.7

Chain	Residue	Details
A	THR145
B	THR145
C	THR145
D	THR145

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P61983

Chain	Residue	Details
A	SER215
B	SER215
C	SER215
D	SER215

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:24275569

Chain	Residue	Details
A	THR234
B	THR234
C	THR234
D	THR234

---