6Z1F

CryoEM structure of Rubisco Activase with its substrate Rubisco from Nostoc sp. (strain PCC7120)

Functional Information from GO Data

Chain	GOid	namespace	contents
1	0005524	molecular_function	ATP binding
1	0016887	molecular_function	ATP hydrolysis activity
2	0005524	molecular_function	ATP binding
2	0016887	molecular_function	ATP hydrolysis activity
3	0005524	molecular_function	ATP binding
3	0016887	molecular_function	ATP hydrolysis activity
4	0005524	molecular_function	ATP binding
4	0016887	molecular_function	ATP hydrolysis activity
5	0005524	molecular_function	ATP binding
5	0016887	molecular_function	ATP hydrolysis activity
6	0005524	molecular_function	ATP binding
6	0016887	molecular_function	ATP hydrolysis activity
A	0000287	molecular_function	magnesium ion binding
A	0004497	molecular_function	monooxygenase activity
A	0009853	biological_process	photorespiration
A	0015977	biological_process	carbon fixation
A	0015979	biological_process	photosynthesis
A	0016491	molecular_function	oxidoreductase activity
A	0016829	molecular_function	lyase activity
A	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
A	0019253	biological_process	reductive pentose-phosphate cycle
A	0031470	cellular_component	carboxysome
A	0046872	molecular_function	metal ion binding
B	0000287	molecular_function	magnesium ion binding
B	0004497	molecular_function	monooxygenase activity
B	0009853	biological_process	photorespiration
B	0015977	biological_process	carbon fixation
B	0015979	biological_process	photosynthesis
B	0016491	molecular_function	oxidoreductase activity
B	0016829	molecular_function	lyase activity
B	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
B	0019253	biological_process	reductive pentose-phosphate cycle
B	0031470	cellular_component	carboxysome
B	0046872	molecular_function	metal ion binding
C	0000287	molecular_function	magnesium ion binding
C	0004497	molecular_function	monooxygenase activity
C	0009853	biological_process	photorespiration
C	0015977	biological_process	carbon fixation
C	0015979	biological_process	photosynthesis
C	0016491	molecular_function	oxidoreductase activity
C	0016829	molecular_function	lyase activity
C	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
C	0019253	biological_process	reductive pentose-phosphate cycle
C	0031470	cellular_component	carboxysome
C	0046872	molecular_function	metal ion binding
D	0000287	molecular_function	magnesium ion binding
D	0004497	molecular_function	monooxygenase activity
D	0009853	biological_process	photorespiration
D	0015977	biological_process	carbon fixation
D	0015979	biological_process	photosynthesis
D	0016491	molecular_function	oxidoreductase activity
D	0016829	molecular_function	lyase activity
D	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
D	0019253	biological_process	reductive pentose-phosphate cycle
D	0031470	cellular_component	carboxysome
D	0046872	molecular_function	metal ion binding
E	0000287	molecular_function	magnesium ion binding
E	0004497	molecular_function	monooxygenase activity
E	0009853	biological_process	photorespiration
E	0015977	biological_process	carbon fixation
E	0015979	biological_process	photosynthesis
E	0016491	molecular_function	oxidoreductase activity
E	0016829	molecular_function	lyase activity
E	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
E	0019253	biological_process	reductive pentose-phosphate cycle
E	0031470	cellular_component	carboxysome
E	0046872	molecular_function	metal ion binding
F	0000287	molecular_function	magnesium ion binding
F	0004497	molecular_function	monooxygenase activity
F	0009853	biological_process	photorespiration
F	0015977	biological_process	carbon fixation
F	0015979	biological_process	photosynthesis
F	0016491	molecular_function	oxidoreductase activity
F	0016829	molecular_function	lyase activity
F	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
F	0019253	biological_process	reductive pentose-phosphate cycle
F	0031470	cellular_component	carboxysome
F	0046872	molecular_function	metal ion binding
G	0000287	molecular_function	magnesium ion binding
G	0004497	molecular_function	monooxygenase activity
G	0009853	biological_process	photorespiration
G	0015977	biological_process	carbon fixation
G	0015979	biological_process	photosynthesis
G	0016491	molecular_function	oxidoreductase activity
G	0016829	molecular_function	lyase activity
G	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
G	0019253	biological_process	reductive pentose-phosphate cycle
G	0031470	cellular_component	carboxysome
G	0046872	molecular_function	metal ion binding
H	0000287	molecular_function	magnesium ion binding
H	0004497	molecular_function	monooxygenase activity
H	0009853	biological_process	photorespiration
H	0015977	biological_process	carbon fixation
H	0015979	biological_process	photosynthesis
H	0016491	molecular_function	oxidoreductase activity
H	0016829	molecular_function	lyase activity
H	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
H	0019253	biological_process	reductive pentose-phosphate cycle
H	0031470	cellular_component	carboxysome
H	0046872	molecular_function	metal ion binding
I	0009853	biological_process	photorespiration
I	0015977	biological_process	carbon fixation
I	0015979	biological_process	photosynthesis
I	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
I	0019253	biological_process	reductive pentose-phosphate cycle
I	0031470	cellular_component	carboxysome
J	0009853	biological_process	photorespiration
J	0015977	biological_process	carbon fixation
J	0015979	biological_process	photosynthesis
J	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
J	0019253	biological_process	reductive pentose-phosphate cycle
J	0031470	cellular_component	carboxysome
K	0009853	biological_process	photorespiration
K	0015977	biological_process	carbon fixation
K	0015979	biological_process	photosynthesis
K	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
K	0019253	biological_process	reductive pentose-phosphate cycle
K	0031470	cellular_component	carboxysome
L	0009853	biological_process	photorespiration
L	0015977	biological_process	carbon fixation
L	0015979	biological_process	photosynthesis
L	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
L	0019253	biological_process	reductive pentose-phosphate cycle
L	0031470	cellular_component	carboxysome
M	0009853	biological_process	photorespiration
M	0015977	biological_process	carbon fixation
M	0015979	biological_process	photosynthesis
M	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
M	0019253	biological_process	reductive pentose-phosphate cycle
M	0031470	cellular_component	carboxysome
N	0009853	biological_process	photorespiration
N	0015977	biological_process	carbon fixation
N	0015979	biological_process	photosynthesis
N	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
N	0019253	biological_process	reductive pentose-phosphate cycle
N	0031470	cellular_component	carboxysome
O	0009853	biological_process	photorespiration
O	0015977	biological_process	carbon fixation
O	0015979	biological_process	photosynthesis
O	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
O	0019253	biological_process	reductive pentose-phosphate cycle
O	0031470	cellular_component	carboxysome
P	0009853	biological_process	photorespiration
P	0015977	biological_process	carbon fixation
P	0015979	biological_process	photosynthesis
P	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
P	0019253	biological_process	reductive pentose-phosphate cycle
P	0031470	cellular_component	carboxysome

Functional Information from PDB Data

site_id	AC1
Number of Residues	12
Details	binding site for residue ADP 1 301

Chain	Residue
1	TYR3
1	GLN46
1	ILE188
1	ASP216
1	TYR4
1	ARG38
1	GLY40
1	GLU41
1	GLY42
1	LYS43
1	THR44
1	PHE45

---

site_id	AC2
Number of Residues	16
Details	binding site for residue AGS 2 301

Chain	Residue
2	TYR3
2	TYR4
2	LYS39
2	GLY40
2	GLU41
2	GLY42
2	LYS43
2	THR44
2	PHE45
2	ILE215
2	ASP216
2	SER219
2	MG302
3	PRO166
3	ARG169
3	ARG172

---

site_id	AC3
Number of Residues	2
Details	binding site for residue MG 2 302

Chain	Residue
2	THR44
2	AGS301

---

site_id	AC4
Number of Residues	19
Details	binding site for residue AGS 3 301

Chain	Residue
3	TYR3
3	TYR4
3	LYS39
3	GLY40
3	GLU41
3	GLY42
3	LYS43
3	THR44
3	PHE45
3	ILE188
3	ILE192
3	ILE215
3	ASP216
3	SER219
3	MG302
4	ARG28
4	PRO166
4	ARG169
4	ARG172

---

site_id	AC5
Number of Residues	3
Details	binding site for residue MG 3 302

Chain	Residue
3	THR44
3	AGS301
4	ARG172

---

site_id	AC6
Number of Residues	17
Details	binding site for residue AGS 4 301

Chain	Residue
4	TYR3
4	TYR4
4	GLY40
4	GLU41
4	GLY42
4	LYS43
4	THR44
4	PHE45
4	GLN46
4	ILE188
4	ILE215
4	ASP216
4	SER219
4	MG302
5	ARG28
5	ARG169
5	ARG172

---

site_id	AC7
Number of Residues	3
Details	binding site for residue MG 4 302

Chain	Residue
4	THR44
4	AGS301
5	ARG169

---

site_id	AC8
Number of Residues	20
Details	binding site for residue AGS 5 301

Chain	Residue
5	TYR3
5	TYR4
5	ARG38
5	LYS39
5	GLY40
5	GLU41
5	GLY42
5	LYS43
5	THR44
5	PHE45
5	GLN46
5	ILE188
5	ILE192
5	ILE215
5	ASP216
5	SER219
5	MG302
6	ARG28
6	ARG169
6	ARG172

---

site_id	AC9
Number of Residues	3
Details	binding site for residue MG 5 302

Chain	Residue
5	THR44
5	AGS301
6	ASP132

---

site_id	AD1
Number of Residues	16
Details	binding site for residue AGS 6 301

Chain	Residue
6	GLU41
6	GLY42
6	LYS43
6	THR44
6	PHE45
6	ASP102
6	THR156
6	ASN158
6	ILE188
6	ILE215
6	ASP216
6	SER219
1	ARG169
6	TYR3
6	LYS39
6	GLY40

---

site_id	AD2
Number of Residues	4
Details	binding site for residue MG A 501

Chain	Residue
A	KCX202
A	ASP204
A	GLU205
A	CAP502

---

site_id	AD3
Number of Residues	18
Details	binding site for residue CAP A 502

Chain	Residue
A	LYS176
A	LYS178
A	KCX202
A	GLU205
A	HIS295
A	ARG296
A	HIS299
A	HIS328
A	LYS335
A	LEU336
A	SER380
A	GLY381
A	GLY382
A	GLY404
A	GLY405
A	MG501
B	GLU61
B	TRP67

---

site_id	AD4
Number of Residues	2
Details	binding site for residue MG B 501

Chain	Residue
B	KCX202
B	GLU205

---

site_id	AD5
Number of Residues	5
Details	binding site for residue MG C 501

Chain	Residue
C	LYS176
C	KCX202
C	ASP204
C	GLU205
C	CAP502

---

site_id	AD6
Number of Residues	17
Details	binding site for residue CAP C 502

Chain	Residue
C	LYS176
C	KCX202
C	GLU205
C	HIS295
C	ARG296
C	HIS328
C	LYS335
C	LEU336
C	SER380
C	GLY381
C	GLY382
C	GLY404
C	GLY405
C	MG501
D	GLU61
D	TRP67
D	ASN124

---

site_id	AD7
Number of Residues	5
Details	binding site for residue MG D 501

Chain	Residue
D	LYS176
D	KCX202
D	ASP204
D	GLU205
D	CAP502

---

site_id	AD8
Number of Residues	17
Details	binding site for residue CAP D 502

Chain	Residue
C	GLU61
C	TRP67
C	ASN124
D	LYS176
D	KCX202
D	GLU205
D	HIS295
D	ARG296
D	HIS328
D	LYS335
D	LEU336
D	SER380
D	GLY381
D	GLY382
D	GLY404
D	GLY405
D	MG501

---

site_id	AD9
Number of Residues	5
Details	binding site for residue MG E 501

Chain	Residue
E	LYS176
E	KCX202
E	ASP204
E	GLU205
E	CAP502

---

site_id	AE1
Number of Residues	17
Details	binding site for residue CAP E 502

Chain	Residue
E	LYS176
E	KCX202
E	GLU205
E	HIS295
E	ARG296
E	HIS328
E	LYS335
E	LEU336
E	SER380
E	GLY381
E	GLY382
E	GLY404
E	GLY405
E	MG501
F	GLU61
F	TRP67
F	ASN124

---

site_id	AE2
Number of Residues	5
Details	binding site for residue MG F 501

Chain	Residue
F	LYS176
F	KCX202
F	ASP204
F	GLU205
F	CAP502

---

site_id	AE3
Number of Residues	17
Details	binding site for residue CAP F 502

Chain	Residue
E	GLU61
E	TRP67
E	ASN124
F	LYS176
F	KCX202
F	GLU205
F	HIS295
F	ARG296
F	HIS328
F	LYS335
F	LEU336
F	SER380
F	GLY381
F	GLY382
F	GLY404
F	GLY405
F	MG501

---

site_id	AE4
Number of Residues	5
Details	binding site for residue MG G 501

Chain	Residue
G	LYS176
G	KCX202
G	ASP204
G	GLU205
G	CAP502

---

site_id	AE5
Number of Residues	17
Details	binding site for residue CAP G 502

Chain	Residue
G	LYS176
G	KCX202
G	GLU205
G	HIS295
G	ARG296
G	HIS328
G	LYS335
G	LEU336
G	SER380
G	GLY381
G	GLY382
G	GLY404
G	GLY405
G	MG501
H	GLU61
H	TRP67
H	ASN124

---

site_id	AE6
Number of Residues	5
Details	binding site for residue MG H 501

Chain	Residue
H	LYS176
H	KCX202
H	ASP204
H	GLU205
H	CAP502

---

site_id	AE7
Number of Residues	17
Details	binding site for residue CAP H 502

Chain	Residue
G	GLU61
G	TRP67
G	ASN124
H	LYS176
H	KCX202
H	GLU205
H	HIS295
H	ARG296
H	HIS328
H	LYS335
H	LEU336
H	SER380
H	GLY381
H	GLY382
H	GLY404
H	GLY405
H	MG501

---

Functional Information from PROSITE/UniProt

site_id	PS00157
Number of Residues	9
Details	RUBISCO_LARGE Ribulose bisphosphate carboxylase large chain active site. GlDFtKdDE

Chain	Residue	Details
A	GLY197-GLU205

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	42
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	16
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_01338","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	Binding site: {"description":"in homodimeric partner","evidences":[{"source":"HAMAP-Rule","id":"MF_01338","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	56
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01338","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	Binding site: {"description":"via carbamate group","evidences":[{"source":"HAMAP-Rule","id":"MF_01338","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	Site: {"description":"Transition state stabilizer","evidences":[{"source":"HAMAP-Rule","id":"MF_01338","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	8
Details	Modified residue: {"description":"N6-carboxylysine","evidences":[{"source":"HAMAP-Rule","id":"MF_01338","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---