6Z1F
CryoEM structure of Rubisco Activase with its substrate Rubisco from Nostoc sp. (strain PCC7120)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| 1 | 0005524 | molecular_function | ATP binding |
| 1 | 0016887 | molecular_function | ATP hydrolysis activity |
| 2 | 0005524 | molecular_function | ATP binding |
| 2 | 0016887 | molecular_function | ATP hydrolysis activity |
| 3 | 0005524 | molecular_function | ATP binding |
| 3 | 0016887 | molecular_function | ATP hydrolysis activity |
| 4 | 0005524 | molecular_function | ATP binding |
| 4 | 0016887 | molecular_function | ATP hydrolysis activity |
| 5 | 0005524 | molecular_function | ATP binding |
| 5 | 0016887 | molecular_function | ATP hydrolysis activity |
| 6 | 0005524 | molecular_function | ATP binding |
| 6 | 0016887 | molecular_function | ATP hydrolysis activity |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0004497 | molecular_function | monooxygenase activity |
| A | 0009853 | biological_process | photorespiration |
| A | 0015977 | biological_process | carbon fixation |
| A | 0015979 | biological_process | photosynthesis |
| A | 0016829 | molecular_function | lyase activity |
| A | 0016984 | molecular_function | ribulose-bisphosphate carboxylase activity |
| A | 0019253 | biological_process | reductive pentose-phosphate cycle |
| A | 0031470 | cellular_component | carboxysome |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0004497 | molecular_function | monooxygenase activity |
| B | 0009853 | biological_process | photorespiration |
| B | 0015977 | biological_process | carbon fixation |
| B | 0015979 | biological_process | photosynthesis |
| B | 0016829 | molecular_function | lyase activity |
| B | 0016984 | molecular_function | ribulose-bisphosphate carboxylase activity |
| B | 0019253 | biological_process | reductive pentose-phosphate cycle |
| B | 0031470 | cellular_component | carboxysome |
| B | 0046872 | molecular_function | metal ion binding |
| C | 0000287 | molecular_function | magnesium ion binding |
| C | 0004497 | molecular_function | monooxygenase activity |
| C | 0009853 | biological_process | photorespiration |
| C | 0015977 | biological_process | carbon fixation |
| C | 0015979 | biological_process | photosynthesis |
| C | 0016829 | molecular_function | lyase activity |
| C | 0016984 | molecular_function | ribulose-bisphosphate carboxylase activity |
| C | 0019253 | biological_process | reductive pentose-phosphate cycle |
| C | 0031470 | cellular_component | carboxysome |
| C | 0046872 | molecular_function | metal ion binding |
| D | 0000287 | molecular_function | magnesium ion binding |
| D | 0004497 | molecular_function | monooxygenase activity |
| D | 0009853 | biological_process | photorespiration |
| D | 0015977 | biological_process | carbon fixation |
| D | 0015979 | biological_process | photosynthesis |
| D | 0016829 | molecular_function | lyase activity |
| D | 0016984 | molecular_function | ribulose-bisphosphate carboxylase activity |
| D | 0019253 | biological_process | reductive pentose-phosphate cycle |
| D | 0031470 | cellular_component | carboxysome |
| D | 0046872 | molecular_function | metal ion binding |
| E | 0000287 | molecular_function | magnesium ion binding |
| E | 0004497 | molecular_function | monooxygenase activity |
| E | 0009853 | biological_process | photorespiration |
| E | 0015977 | biological_process | carbon fixation |
| E | 0015979 | biological_process | photosynthesis |
| E | 0016829 | molecular_function | lyase activity |
| E | 0016984 | molecular_function | ribulose-bisphosphate carboxylase activity |
| E | 0019253 | biological_process | reductive pentose-phosphate cycle |
| E | 0031470 | cellular_component | carboxysome |
| E | 0046872 | molecular_function | metal ion binding |
| F | 0000287 | molecular_function | magnesium ion binding |
| F | 0004497 | molecular_function | monooxygenase activity |
| F | 0009853 | biological_process | photorespiration |
| F | 0015977 | biological_process | carbon fixation |
| F | 0015979 | biological_process | photosynthesis |
| F | 0016829 | molecular_function | lyase activity |
| F | 0016984 | molecular_function | ribulose-bisphosphate carboxylase activity |
| F | 0019253 | biological_process | reductive pentose-phosphate cycle |
| F | 0031470 | cellular_component | carboxysome |
| F | 0046872 | molecular_function | metal ion binding |
| G | 0000287 | molecular_function | magnesium ion binding |
| G | 0004497 | molecular_function | monooxygenase activity |
| G | 0009853 | biological_process | photorespiration |
| G | 0015977 | biological_process | carbon fixation |
| G | 0015979 | biological_process | photosynthesis |
| G | 0016829 | molecular_function | lyase activity |
| G | 0016984 | molecular_function | ribulose-bisphosphate carboxylase activity |
| G | 0019253 | biological_process | reductive pentose-phosphate cycle |
| G | 0031470 | cellular_component | carboxysome |
| G | 0046872 | molecular_function | metal ion binding |
| H | 0000287 | molecular_function | magnesium ion binding |
| H | 0004497 | molecular_function | monooxygenase activity |
| H | 0009853 | biological_process | photorespiration |
| H | 0015977 | biological_process | carbon fixation |
| H | 0015979 | biological_process | photosynthesis |
| H | 0016829 | molecular_function | lyase activity |
| H | 0016984 | molecular_function | ribulose-bisphosphate carboxylase activity |
| H | 0019253 | biological_process | reductive pentose-phosphate cycle |
| H | 0031470 | cellular_component | carboxysome |
| H | 0046872 | molecular_function | metal ion binding |
| I | 0009853 | biological_process | photorespiration |
| I | 0015977 | biological_process | carbon fixation |
| I | 0015979 | biological_process | photosynthesis |
| I | 0016984 | molecular_function | ribulose-bisphosphate carboxylase activity |
| I | 0019253 | biological_process | reductive pentose-phosphate cycle |
| I | 0031470 | cellular_component | carboxysome |
| J | 0009853 | biological_process | photorespiration |
| J | 0015977 | biological_process | carbon fixation |
| J | 0015979 | biological_process | photosynthesis |
| J | 0016984 | molecular_function | ribulose-bisphosphate carboxylase activity |
| J | 0019253 | biological_process | reductive pentose-phosphate cycle |
| J | 0031470 | cellular_component | carboxysome |
| K | 0009853 | biological_process | photorespiration |
| K | 0015977 | biological_process | carbon fixation |
| K | 0015979 | biological_process | photosynthesis |
| K | 0016984 | molecular_function | ribulose-bisphosphate carboxylase activity |
| K | 0019253 | biological_process | reductive pentose-phosphate cycle |
| K | 0031470 | cellular_component | carboxysome |
| L | 0009853 | biological_process | photorespiration |
| L | 0015977 | biological_process | carbon fixation |
| L | 0015979 | biological_process | photosynthesis |
| L | 0016984 | molecular_function | ribulose-bisphosphate carboxylase activity |
| L | 0019253 | biological_process | reductive pentose-phosphate cycle |
| L | 0031470 | cellular_component | carboxysome |
| M | 0009853 | biological_process | photorespiration |
| M | 0015977 | biological_process | carbon fixation |
| M | 0015979 | biological_process | photosynthesis |
| M | 0016984 | molecular_function | ribulose-bisphosphate carboxylase activity |
| M | 0019253 | biological_process | reductive pentose-phosphate cycle |
| M | 0031470 | cellular_component | carboxysome |
| N | 0009853 | biological_process | photorespiration |
| N | 0015977 | biological_process | carbon fixation |
| N | 0015979 | biological_process | photosynthesis |
| N | 0016984 | molecular_function | ribulose-bisphosphate carboxylase activity |
| N | 0019253 | biological_process | reductive pentose-phosphate cycle |
| N | 0031470 | cellular_component | carboxysome |
| O | 0009853 | biological_process | photorespiration |
| O | 0015977 | biological_process | carbon fixation |
| O | 0015979 | biological_process | photosynthesis |
| O | 0016984 | molecular_function | ribulose-bisphosphate carboxylase activity |
| O | 0019253 | biological_process | reductive pentose-phosphate cycle |
| O | 0031470 | cellular_component | carboxysome |
| P | 0009853 | biological_process | photorespiration |
| P | 0015977 | biological_process | carbon fixation |
| P | 0015979 | biological_process | photosynthesis |
| P | 0016984 | molecular_function | ribulose-bisphosphate carboxylase activity |
| P | 0019253 | biological_process | reductive pentose-phosphate cycle |
| P | 0031470 | cellular_component | carboxysome |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 12 |
| Details | binding site for residue ADP 1 301 |
| Chain | Residue |
| 1 | TYR3 |
| 1 | GLN46 |
| 1 | ILE188 |
| 1 | ASP216 |
| 1 | TYR4 |
| 1 | ARG38 |
| 1 | GLY40 |
| 1 | GLU41 |
| 1 | GLY42 |
| 1 | LYS43 |
| 1 | THR44 |
| 1 | PHE45 |
| site_id | AC2 |
| Number of Residues | 16 |
| Details | binding site for residue AGS 2 301 |
| Chain | Residue |
| 2 | TYR3 |
| 2 | TYR4 |
| 2 | LYS39 |
| 2 | GLY40 |
| 2 | GLU41 |
| 2 | GLY42 |
| 2 | LYS43 |
| 2 | THR44 |
| 2 | PHE45 |
| 2 | ILE215 |
| 2 | ASP216 |
| 2 | SER219 |
| 2 | MG302 |
| 3 | PRO166 |
| 3 | ARG169 |
| 3 | ARG172 |
| site_id | AC3 |
| Number of Residues | 2 |
| Details | binding site for residue MG 2 302 |
| Chain | Residue |
| 2 | THR44 |
| 2 | AGS301 |
| site_id | AC4 |
| Number of Residues | 19 |
| Details | binding site for residue AGS 3 301 |
| Chain | Residue |
| 3 | TYR3 |
| 3 | TYR4 |
| 3 | LYS39 |
| 3 | GLY40 |
| 3 | GLU41 |
| 3 | GLY42 |
| 3 | LYS43 |
| 3 | THR44 |
| 3 | PHE45 |
| 3 | ILE188 |
| 3 | ILE192 |
| 3 | ILE215 |
| 3 | ASP216 |
| 3 | SER219 |
| 3 | MG302 |
| 4 | ARG28 |
| 4 | PRO166 |
| 4 | ARG169 |
| 4 | ARG172 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | binding site for residue MG 3 302 |
| Chain | Residue |
| 3 | THR44 |
| 3 | AGS301 |
| 4 | ARG172 |
| site_id | AC6 |
| Number of Residues | 17 |
| Details | binding site for residue AGS 4 301 |
| Chain | Residue |
| 4 | TYR3 |
| 4 | TYR4 |
| 4 | GLY40 |
| 4 | GLU41 |
| 4 | GLY42 |
| 4 | LYS43 |
| 4 | THR44 |
| 4 | PHE45 |
| 4 | GLN46 |
| 4 | ILE188 |
| 4 | ILE215 |
| 4 | ASP216 |
| 4 | SER219 |
| 4 | MG302 |
| 5 | ARG28 |
| 5 | ARG169 |
| 5 | ARG172 |
| site_id | AC7 |
| Number of Residues | 3 |
| Details | binding site for residue MG 4 302 |
| Chain | Residue |
| 4 | THR44 |
| 4 | AGS301 |
| 5 | ARG169 |
| site_id | AC8 |
| Number of Residues | 20 |
| Details | binding site for residue AGS 5 301 |
| Chain | Residue |
| 5 | TYR3 |
| 5 | TYR4 |
| 5 | ARG38 |
| 5 | LYS39 |
| 5 | GLY40 |
| 5 | GLU41 |
| 5 | GLY42 |
| 5 | LYS43 |
| 5 | THR44 |
| 5 | PHE45 |
| 5 | GLN46 |
| 5 | ILE188 |
| 5 | ILE192 |
| 5 | ILE215 |
| 5 | ASP216 |
| 5 | SER219 |
| 5 | MG302 |
| 6 | ARG28 |
| 6 | ARG169 |
| 6 | ARG172 |
| site_id | AC9 |
| Number of Residues | 3 |
| Details | binding site for residue MG 5 302 |
| Chain | Residue |
| 5 | THR44 |
| 5 | AGS301 |
| 6 | ASP132 |
| site_id | AD1 |
| Number of Residues | 16 |
| Details | binding site for residue AGS 6 301 |
| Chain | Residue |
| 6 | GLU41 |
| 6 | GLY42 |
| 6 | LYS43 |
| 6 | THR44 |
| 6 | PHE45 |
| 6 | ASP102 |
| 6 | THR156 |
| 6 | ASN158 |
| 6 | ILE188 |
| 6 | ILE215 |
| 6 | ASP216 |
| 6 | SER219 |
| 1 | ARG169 |
| 6 | TYR3 |
| 6 | LYS39 |
| 6 | GLY40 |
| site_id | AD2 |
| Number of Residues | 4 |
| Details | binding site for residue MG A 501 |
| Chain | Residue |
| A | KCX202 |
| A | ASP204 |
| A | GLU205 |
| A | CAP502 |
| site_id | AD3 |
| Number of Residues | 18 |
| Details | binding site for residue CAP A 502 |
| Chain | Residue |
| A | LYS176 |
| A | LYS178 |
| A | KCX202 |
| A | GLU205 |
| A | HIS295 |
| A | ARG296 |
| A | HIS299 |
| A | HIS328 |
| A | LYS335 |
| A | LEU336 |
| A | SER380 |
| A | GLY381 |
| A | GLY382 |
| A | GLY404 |
| A | GLY405 |
| A | MG501 |
| B | GLU61 |
| B | TRP67 |
| site_id | AD4 |
| Number of Residues | 2 |
| Details | binding site for residue MG B 501 |
| Chain | Residue |
| B | KCX202 |
| B | GLU205 |
| site_id | AD5 |
| Number of Residues | 5 |
| Details | binding site for residue MG C 501 |
| Chain | Residue |
| C | LYS176 |
| C | KCX202 |
| C | ASP204 |
| C | GLU205 |
| C | CAP502 |
| site_id | AD6 |
| Number of Residues | 17 |
| Details | binding site for residue CAP C 502 |
| Chain | Residue |
| C | LYS176 |
| C | KCX202 |
| C | GLU205 |
| C | HIS295 |
| C | ARG296 |
| C | HIS328 |
| C | LYS335 |
| C | LEU336 |
| C | SER380 |
| C | GLY381 |
| C | GLY382 |
| C | GLY404 |
| C | GLY405 |
| C | MG501 |
| D | GLU61 |
| D | TRP67 |
| D | ASN124 |
| site_id | AD7 |
| Number of Residues | 5 |
| Details | binding site for residue MG D 501 |
| Chain | Residue |
| D | LYS176 |
| D | KCX202 |
| D | ASP204 |
| D | GLU205 |
| D | CAP502 |
| site_id | AD8 |
| Number of Residues | 17 |
| Details | binding site for residue CAP D 502 |
| Chain | Residue |
| C | GLU61 |
| C | TRP67 |
| C | ASN124 |
| D | LYS176 |
| D | KCX202 |
| D | GLU205 |
| D | HIS295 |
| D | ARG296 |
| D | HIS328 |
| D | LYS335 |
| D | LEU336 |
| D | SER380 |
| D | GLY381 |
| D | GLY382 |
| D | GLY404 |
| D | GLY405 |
| D | MG501 |
| site_id | AD9 |
| Number of Residues | 5 |
| Details | binding site for residue MG E 501 |
| Chain | Residue |
| E | LYS176 |
| E | KCX202 |
| E | ASP204 |
| E | GLU205 |
| E | CAP502 |
| site_id | AE1 |
| Number of Residues | 17 |
| Details | binding site for residue CAP E 502 |
| Chain | Residue |
| E | LYS176 |
| E | KCX202 |
| E | GLU205 |
| E | HIS295 |
| E | ARG296 |
| E | HIS328 |
| E | LYS335 |
| E | LEU336 |
| E | SER380 |
| E | GLY381 |
| E | GLY382 |
| E | GLY404 |
| E | GLY405 |
| E | MG501 |
| F | GLU61 |
| F | TRP67 |
| F | ASN124 |
| site_id | AE2 |
| Number of Residues | 5 |
| Details | binding site for residue MG F 501 |
| Chain | Residue |
| F | LYS176 |
| F | KCX202 |
| F | ASP204 |
| F | GLU205 |
| F | CAP502 |
| site_id | AE3 |
| Number of Residues | 17 |
| Details | binding site for residue CAP F 502 |
| Chain | Residue |
| E | GLU61 |
| E | TRP67 |
| E | ASN124 |
| F | LYS176 |
| F | KCX202 |
| F | GLU205 |
| F | HIS295 |
| F | ARG296 |
| F | HIS328 |
| F | LYS335 |
| F | LEU336 |
| F | SER380 |
| F | GLY381 |
| F | GLY382 |
| F | GLY404 |
| F | GLY405 |
| F | MG501 |
| site_id | AE4 |
| Number of Residues | 5 |
| Details | binding site for residue MG G 501 |
| Chain | Residue |
| G | LYS176 |
| G | KCX202 |
| G | ASP204 |
| G | GLU205 |
| G | CAP502 |
| site_id | AE5 |
| Number of Residues | 17 |
| Details | binding site for residue CAP G 502 |
| Chain | Residue |
| G | LYS176 |
| G | KCX202 |
| G | GLU205 |
| G | HIS295 |
| G | ARG296 |
| G | HIS328 |
| G | LYS335 |
| G | LEU336 |
| G | SER380 |
| G | GLY381 |
| G | GLY382 |
| G | GLY404 |
| G | GLY405 |
| G | MG501 |
| H | GLU61 |
| H | TRP67 |
| H | ASN124 |
| site_id | AE6 |
| Number of Residues | 5 |
| Details | binding site for residue MG H 501 |
| Chain | Residue |
| H | LYS176 |
| H | KCX202 |
| H | ASP204 |
| H | GLU205 |
| H | CAP502 |
| site_id | AE7 |
| Number of Residues | 17 |
| Details | binding site for residue CAP H 502 |
| Chain | Residue |
| G | GLU61 |
| G | TRP67 |
| G | ASN124 |
| H | LYS176 |
| H | KCX202 |
| H | GLU205 |
| H | HIS295 |
| H | ARG296 |
| H | HIS328 |
| H | LYS335 |
| H | LEU336 |
| H | SER380 |
| H | GLY381 |
| H | GLY382 |
| H | GLY404 |
| H | GLY405 |
| H | MG501 |
Functional Information from PROSITE/UniProt
| site_id | PS00157 |
| Number of Residues | 9 |
| Details | RUBISCO_LARGE Ribulose bisphosphate carboxylase large chain active site. GlDFtKdDE |
| Chain | Residue | Details |
| A | GLY197-GLU205 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 16 |
| Details | ACT_SITE: Proton acceptor => ECO:0000255|HAMAP-Rule:MF_01338 |
| Chain | Residue | Details |
| A | LYS176 | |
| E | HIS295 | |
| F | LYS176 | |
| F | HIS295 | |
| G | LYS176 | |
| G | HIS295 | |
| H | LYS176 | |
| H | HIS295 | |
| A | HIS295 | |
| B | LYS176 | |
| B | HIS295 | |
| C | LYS176 | |
| C | HIS295 | |
| D | LYS176 | |
| D | HIS295 | |
| E | LYS176 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | BINDING: in homodimeric partner => ECO:0000255|HAMAP-Rule:MF_01338 |
| Chain | Residue | Details |
| A | ASN124 | |
| B | ASN124 | |
| C | ASN124 | |
| D | ASN124 | |
| E | ASN124 | |
| F | ASN124 | |
| G | ASN124 | |
| H | ASN124 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 56 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01338 |
| Chain | Residue | Details |
| A | THR174 | |
| B | ASP204 | |
| B | GLU205 | |
| B | ARG296 | |
| B | HIS328 | |
| B | SER380 | |
| C | THR174 | |
| C | LYS178 | |
| C | ASP204 | |
| C | GLU205 | |
| C | ARG296 | |
| A | LYS178 | |
| C | HIS328 | |
| C | SER380 | |
| D | THR174 | |
| D | LYS178 | |
| D | ASP204 | |
| D | GLU205 | |
| D | ARG296 | |
| D | HIS328 | |
| D | SER380 | |
| E | THR174 | |
| A | ASP204 | |
| E | LYS178 | |
| E | ASP204 | |
| E | GLU205 | |
| E | ARG296 | |
| E | HIS328 | |
| E | SER380 | |
| F | THR174 | |
| F | LYS178 | |
| F | ASP204 | |
| F | GLU205 | |
| A | GLU205 | |
| F | ARG296 | |
| F | HIS328 | |
| F | SER380 | |
| G | THR174 | |
| G | LYS178 | |
| G | ASP204 | |
| G | GLU205 | |
| G | ARG296 | |
| G | HIS328 | |
| G | SER380 | |
| A | ARG296 | |
| H | THR174 | |
| H | LYS178 | |
| H | ASP204 | |
| H | GLU205 | |
| H | ARG296 | |
| H | HIS328 | |
| H | SER380 | |
| A | HIS328 | |
| A | SER380 | |
| B | THR174 | |
| B | LYS178 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 8 |
| Details | BINDING: via carbamate group => ECO:0000255|HAMAP-Rule:MF_01338 |
| Chain | Residue | Details |
| A | KCX202 | |
| B | KCX202 | |
| C | KCX202 | |
| D | KCX202 | |
| E | KCX202 | |
| F | KCX202 | |
| G | KCX202 | |
| H | KCX202 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 8 |
| Details | SITE: Transition state stabilizer => ECO:0000255|HAMAP-Rule:MF_01338 |
| Chain | Residue | Details |
| A | LYS335 | |
| B | LYS335 | |
| C | LYS335 | |
| D | LYS335 | |
| E | LYS335 | |
| F | LYS335 | |
| G | LYS335 | |
| H | LYS335 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 8 |
| Details | MOD_RES: N6-carboxylysine => ECO:0000255|HAMAP-Rule:MF_01338 |
| Chain | Residue | Details |
| A | KCX202 | |
| B | KCX202 | |
| C | KCX202 | |
| D | KCX202 | |
| E | KCX202 | |
| F | KCX202 | |
| G | KCX202 | |
| H | KCX202 |
