6Y8C
Crystal structure of the cAMP-dependent protein kinase A cocrystallized with ATP and PKI (5-24)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0001669 | cellular_component | acrosomal vesicle |
| A | 0004672 | molecular_function | protein kinase activity |
| A | 0004674 | molecular_function | protein serine/threonine kinase activity |
| A | 0004691 | molecular_function | cAMP-dependent protein kinase activity |
| A | 0004712 | molecular_function | protein serine/threonine/tyrosine kinase activity |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005811 | cellular_component | lipid droplet |
| A | 0005813 | cellular_component | centrosome |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0005930 | cellular_component | axoneme |
| A | 0005952 | cellular_component | cAMP-dependent protein kinase complex |
| A | 0006338 | biological_process | chromatin remodeling |
| A | 0006397 | biological_process | mRNA processing |
| A | 0006468 | biological_process | protein phosphorylation |
| A | 0007189 | biological_process | adenylate cyclase-activating G protein-coupled receptor signaling pathway |
| A | 0007193 | biological_process | adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway |
| A | 0010898 | biological_process | positive regulation of triglyceride catabolic process |
| A | 0016020 | cellular_component | membrane |
| A | 0016301 | molecular_function | kinase activity |
| A | 0016607 | cellular_component | nuclear speck |
| A | 0016740 | molecular_function | transferase activity |
| A | 0019901 | molecular_function | protein kinase binding |
| A | 0019904 | molecular_function | protein domain specific binding |
| A | 0030007 | biological_process | intracellular potassium ion homeostasis |
| A | 0030145 | molecular_function | manganese ion binding |
| A | 0031594 | cellular_component | neuromuscular junction |
| A | 0034237 | molecular_function | protein kinase A regulatory subunit binding |
| A | 0034605 | biological_process | cellular response to heat |
| A | 0036126 | cellular_component | sperm flagellum |
| A | 0044853 | cellular_component | plasma membrane raft |
| A | 0045542 | biological_process | positive regulation of cholesterol biosynthetic process |
| A | 0045667 | biological_process | regulation of osteoblast differentiation |
| A | 0045722 | biological_process | positive regulation of gluconeogenesis |
| A | 0045820 | biological_process | negative regulation of glycolytic process |
| A | 0048240 | biological_process | sperm capacitation |
| A | 0048471 | cellular_component | perinuclear region of cytoplasm |
| A | 0050766 | biological_process | positive regulation of phagocytosis |
| A | 0051726 | biological_process | regulation of cell cycle |
| A | 0061136 | biological_process | regulation of proteasomal protein catabolic process |
| A | 0070507 | biological_process | regulation of microtubule cytoskeleton organization |
| A | 0071333 | biological_process | cellular response to glucose stimulus |
| A | 0071377 | biological_process | cellular response to glucagon stimulus |
| A | 0097546 | cellular_component | ciliary base |
| A | 0098794 | cellular_component | postsynapse |
| A | 0098978 | cellular_component | glutamatergic synapse |
| A | 0099170 | biological_process | postsynaptic modulation of chemical synaptic transmission |
| A | 0106310 | molecular_function | protein serine kinase activity |
| A | 0120186 | biological_process | negative regulation of protein localization to chromatin |
| A | 0140198 | molecular_function | histone H1-4S35 kinase activity |
| A | 1904262 | biological_process | negative regulation of TORC1 signaling |
| A | 1904539 | biological_process | negative regulation of glycolytic process through fructose-6-phosphate |
| A | 2000810 | biological_process | regulation of bicellular tight junction assembly |
| B | 0004862 | molecular_function | cAMP-dependent protein kinase inhibitor activity |
| B | 0006469 | biological_process | negative regulation of protein kinase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | binding site for residue MG A 401 |
| Chain | Residue |
| A | ASN171 |
| A | ASP184 |
| A | ATP402 |
| A | HOH647 |
| A | HOH672 |
| site_id | AC2 |
| Number of Residues | 23 |
| Details | binding site for residue ATP A 402 |
| Chain | Residue |
| A | VAL104 |
| A | MET120 |
| A | GLU121 |
| A | VAL123 |
| A | GLU127 |
| A | GLU170 |
| A | ASN171 |
| A | LEU173 |
| A | THR183 |
| A | ASP184 |
| A | PHE327 |
| A | MG401 |
| A | HOH507 |
| A | HOH521 |
| A | HOH552 |
| A | HOH613 |
| A | HOH647 |
| A | HOH672 |
| A | HOH727 |
| A | GLY52 |
| A | VAL57 |
| A | ALA70 |
| A | LYS72 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | binding site for residue DMS A 403 |
| Chain | Residue |
| A | ASN36 |
| A | ALA38 |
| A | HIS42 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | binding site for residue DMS A 404 |
| Chain | Residue |
| A | THR299 |
| A | THR300 |
| A | ASP301 |
| A | VAL310 |
| A | HOH525 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | binding site for residue DMS A 405 |
| Chain | Residue |
| A | GLU31 |
| A | ARG93 |
| B | HOH211 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | binding site for residue DMS A 406 |
| Chain | Residue |
| A | SER34 |
| A | THR37 |
| A | LEU106 |
| A | GLU107 |
| A | PHE108 |
| A | HOH558 |
| site_id | AC7 |
| Number of Residues | 5 |
| Details | binding site for residue MPD A 407 |
| Chain | Residue |
| A | VAL15 |
| A | PHE18 |
| A | GLU155 |
| A | TYR306 |
| A | HOH640 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | binding site for residue MPD A 408 |
| Chain | Residue |
| A | HIS131 |
| A | ARG134 |
| A | SER325 |
| A | ASN326 |
| A | ASP328 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | binding site for residue DMS B 101 |
| Chain | Residue |
| B | ARG15 |
| B | ARG18 |
| B | ARG19 |
| B | ASN20 |
| B | HOH209 |
Functional Information from PROSITE/UniProt
| site_id | PS00107 |
| Number of Residues | 24 |
| Details | PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGTGSFGRVMlVkhketgnh..........YAMK |
| Chain | Residue | Details |
| A | LEU49-LYS72 |
| site_id | PS00108 |
| Number of Residues | 13 |
| Details | PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. LiYrDLKpeNLLI |
| Chain | Residue | Details |
| A | LEU162-ILE174 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10027","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 18 |
| Details | Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"P17612","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P05132","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphothreonine; by PDPK1","evidences":[{"source":"UniProtKB","id":"P00517","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"P05132","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P00517","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 3 |
| Details | Site: {"description":"Important for inhibition","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
