6X5Z

Bovine Cardiac Myosin in Complex with Chicken Skeletal Actin and Human Cardiac Tropomyosin in the Rigor State

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0001725	cellular_component	stress fiber
A	0005200	molecular_function	structural constituent of cytoskeleton
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005856	cellular_component	cytoskeleton
A	0005865	cellular_component	striated muscle thin filament
A	0005884	cellular_component	actin filament
A	0015629	cellular_component	actin cytoskeleton
A	0016787	molecular_function	hydrolase activity
A	0030240	biological_process	skeletal muscle thin filament assembly
A	0048741	biological_process	skeletal muscle fiber development
B	0000166	molecular_function	nucleotide binding
B	0001725	cellular_component	stress fiber
B	0005200	molecular_function	structural constituent of cytoskeleton
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005856	cellular_component	cytoskeleton
B	0005865	cellular_component	striated muscle thin filament
B	0005884	cellular_component	actin filament
B	0015629	cellular_component	actin cytoskeleton
B	0016787	molecular_function	hydrolase activity
B	0030240	biological_process	skeletal muscle thin filament assembly
B	0048741	biological_process	skeletal muscle fiber development
C	0000166	molecular_function	nucleotide binding
C	0001725	cellular_component	stress fiber
C	0005200	molecular_function	structural constituent of cytoskeleton
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005856	cellular_component	cytoskeleton
C	0005865	cellular_component	striated muscle thin filament
C	0005884	cellular_component	actin filament
C	0015629	cellular_component	actin cytoskeleton
C	0016787	molecular_function	hydrolase activity
C	0030240	biological_process	skeletal muscle thin filament assembly
C	0048741	biological_process	skeletal muscle fiber development
D	0003774	molecular_function	cytoskeletal motor activity
D	0005524	molecular_function	ATP binding
D	0016459	cellular_component	myosin complex
D	0051015	molecular_function	actin filament binding
G	0003774	molecular_function	cytoskeletal motor activity
G	0005524	molecular_function	ATP binding
G	0016459	cellular_component	myosin complex
G	0051015	molecular_function	actin filament binding
J	0003774	molecular_function	cytoskeletal motor activity
J	0005524	molecular_function	ATP binding
J	0016459	cellular_component	myosin complex
J	0051015	molecular_function	actin filament binding
O	0001725	cellular_component	stress fiber
O	0003065	biological_process	positive regulation of heart rate by epinephrine
O	0003779	molecular_function	actin binding
O	0005200	molecular_function	structural constituent of cytoskeleton
O	0005515	molecular_function	protein binding
O	0005829	cellular_component	cytosol
O	0005856	cellular_component	cytoskeleton
O	0005862	cellular_component	muscle thin filament tropomyosin
O	0005884	cellular_component	actin filament
O	0006937	biological_process	regulation of muscle contraction
O	0007010	biological_process	cytoskeleton organization
O	0007015	biological_process	actin filament organization
O	0008016	biological_process	regulation of heart contraction
O	0008092	molecular_function	cytoskeletal protein binding
O	0008307	molecular_function	structural constituent of muscle
O	0008360	biological_process	regulation of cell shape
O	0030017	cellular_component	sarcomere
O	0030049	biological_process	muscle filament sliding
O	0030336	biological_process	negative regulation of cell migration
O	0031529	biological_process	ruffle organization
O	0032059	cellular_component	bleb
O	0032587	cellular_component	ruffle membrane
O	0034614	biological_process	cellular response to reactive oxygen species
O	0042060	biological_process	wound healing
O	0042802	molecular_function	identical protein binding
O	0042803	molecular_function	protein homodimerization activity
O	0045214	biological_process	sarcomere organization
O	0045785	biological_process	positive regulation of cell adhesion
O	0046982	molecular_function	protein heterodimerization activity
O	0051015	molecular_function	actin filament binding
O	0051496	biological_process	positive regulation of stress fiber assembly
O	0055010	biological_process	ventricular cardiac muscle tissue morphogenesis
O	0060048	biological_process	cardiac muscle contraction
O	1904706	biological_process	negative regulation of vascular associated smooth muscle cell proliferation
O	1904753	biological_process	negative regulation of vascular associated smooth muscle cell migration
P	0001725	cellular_component	stress fiber
P	0003065	biological_process	positive regulation of heart rate by epinephrine
P	0003779	molecular_function	actin binding
P	0005200	molecular_function	structural constituent of cytoskeleton
P	0005515	molecular_function	protein binding
P	0005829	cellular_component	cytosol
P	0005856	cellular_component	cytoskeleton
P	0005862	cellular_component	muscle thin filament tropomyosin
P	0005884	cellular_component	actin filament
P	0006937	biological_process	regulation of muscle contraction
P	0007010	biological_process	cytoskeleton organization
P	0007015	biological_process	actin filament organization
P	0008016	biological_process	regulation of heart contraction
P	0008092	molecular_function	cytoskeletal protein binding
P	0008307	molecular_function	structural constituent of muscle
P	0008360	biological_process	regulation of cell shape
P	0030017	cellular_component	sarcomere
P	0030049	biological_process	muscle filament sliding
P	0030336	biological_process	negative regulation of cell migration
P	0031529	biological_process	ruffle organization
P	0032059	cellular_component	bleb
P	0032587	cellular_component	ruffle membrane
P	0034614	biological_process	cellular response to reactive oxygen species
P	0042060	biological_process	wound healing
P	0042802	molecular_function	identical protein binding
P	0042803	molecular_function	protein homodimerization activity
P	0045214	biological_process	sarcomere organization
P	0045785	biological_process	positive regulation of cell adhesion
P	0046982	molecular_function	protein heterodimerization activity
P	0051015	molecular_function	actin filament binding
P	0051496	biological_process	positive regulation of stress fiber assembly
P	0055010	biological_process	ventricular cardiac muscle tissue morphogenesis
P	0060048	biological_process	cardiac muscle contraction
P	1904706	biological_process	negative regulation of vascular associated smooth muscle cell proliferation
P	1904753	biological_process	negative regulation of vascular associated smooth muscle cell migration

Functional Information from PDB Data

site_id	AC1
Number of Residues	11
Details	binding site for residue ADP B 401

Chain	Residue
B	ASP11
B	TYR306
B	MG402
B	GLY13
B	GLY15
B	LEU16
B	LYS18
B	GLY156
B	ASP157
B	GLU214
B	THR303

---

site_id	AC2
Number of Residues	4
Details	binding site for residue MG B 402

Chain	Residue
B	GLY13
B	GLY15
B	LEU16
B	ADP401

---

site_id	AC3
Number of Residues	4
Details	binding site for residue MG A 402

Chain	Residue
A	GLY13
A	GLY15
A	LEU16
A	ADP401

---

site_id	AC4
Number of Residues	4
Details	binding site for residue MG C 402

Chain	Residue
C	GLY13
C	GLY15
C	LEU16
C	ADP401

---

site_id	AC5
Number of Residues	17
Details	binding site for residue ADP A 401

Chain	Residue
A	ASP11
A	GLY13
A	SER14
A	GLY15
A	LEU16
A	LYS18
A	SER155
A	GLY156
A	GLY158
A	VAL159
A	ALA181
A	GLY182
A	ARG183
A	GLU214
A	THR303
A	TYR306
A	MG402

---

site_id	AC6
Number of Residues	17
Details	binding site for residue ADP C 401

Chain	Residue
C	ASP11
C	GLY13
C	SER14
C	GLY15
C	LEU16
C	LYS18
C	SER155
C	GLY156
C	GLY158
C	VAL159
C	ALA181
C	GLY182
C	ARG183
C	GLU214
C	THR303
C	TYR306
C	MG402

---

Functional Information from PROSITE/UniProt

site_id	PS00326
Number of Residues	9
Details	TROPOMYOSIN Tropomyosins signature. LKEAEtRAE

Chain	Residue	Details
O	LEU232-GLU240

---

site_id	PS00406
Number of Residues	11
Details	ACTINS_1 Actins signature 1. YVGDEAQs.KRG

Chain	Residue	Details
B	TYR53-GLY63

---

site_id	PS00432
Number of Residues	9
Details	ACTINS_2 Actins signature 2. WITKqEYDE

Chain	Residue	Details
B	TRP356-GLU364

---

site_id	PS01132
Number of Residues	13
Details	ACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApLNPkaNR

Chain	Residue	Details
B	LEU104-ARG116

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	Modified residue: {"description":"Methionine (R)-sulfoxide","evidences":[{"source":"UniProtKB","id":"P68134","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	3
Details	Modified residue: {"description":"Tele-methylhistidine","evidences":[{"source":"PubMed","id":"12356759","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1MDU","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	Modified residue: {"description":"N6-methyllysine","evidences":[{"source":"UniProtKB","id":"P68133","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	40
Details	Region: {"description":"Disordered","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P04692","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P58771","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	108
Details	Region: {"description":"Actin-binding","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	21
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	3
Details	Modified residue: {"description":"N6,N6,N6-trimethyllysine","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	3
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"P02563","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---