6WN2
Tyrosyl t-RNA Synthetase Mutant from E.coli Complexed with sulfotyrosine
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0004812 | molecular_function | aminoacyl-tRNA ligase activity |
| A | 0004831 | molecular_function | tyrosine-tRNA ligase activity |
| A | 0005524 | molecular_function | ATP binding |
| A | 0006418 | biological_process | tRNA aminoacylation for protein translation |
| A | 0006437 | biological_process | tyrosyl-tRNA aminoacylation |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0004812 | molecular_function | aminoacyl-tRNA ligase activity |
| B | 0004831 | molecular_function | tyrosine-tRNA ligase activity |
| B | 0005524 | molecular_function | ATP binding |
| B | 0006418 | biological_process | tRNA aminoacylation for protein translation |
| B | 0006437 | biological_process | tyrosyl-tRNA aminoacylation |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 17 |
| Details | binding site for residue TYS A 401 |
| Chain | Residue |
| A | TYR37 |
| A | GLN179 |
| A | GLY182 |
| A | LEU186 |
| A | GLN201 |
| A | HOH524 |
| A | HOH550 |
| A | HOH568 |
| A | HOH581 |
| A | GLY39 |
| A | PHE40 |
| A | ASP41 |
| A | VAL71 |
| A | ASP81 |
| A | ASN126 |
| A | PHE129 |
| A | TYR175 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | binding site for residue PG4 A 402 |
| Chain | Residue |
| A | GLU88 |
| A | GLU164 |
| A | HOH528 |
| A | HOH557 |
| B | LYS144 |
| B | SER147 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | binding site for residue PG4 A 403 |
| Chain | Residue |
| A | ASP45 |
| A | TRP100 |
| A | TRP245 |
| A | LYS250 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | binding site for residue PG4 A 404 |
| Chain | Residue |
| A | HIS145 |
| A | PHE146 |
| A | SER147 |
| A | GLN150 |
| A | ARG214 |
| A | HOH502 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | binding site for residue NA A 405 |
| Chain | Residue |
| A | SO4408 |
| A | HOH582 |
| A | HOH593 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 406 |
| Chain | Residue |
| A | ILE79 |
| A | ARG89 |
| A | LEU91 |
| A | GLU172 |
| A | HOH522 |
| A | HOH575 |
| site_id | AC7 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 A 407 |
| Chain | Residue |
| A | LYS85 |
| A | ALA86 |
| A | ALA87 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | binding site for residue SO4 A 408 |
| Chain | Residue |
| A | LYS154 |
| A | TRP202 |
| A | GLY203 |
| A | THR206 |
| A | NA405 |
| site_id | AC9 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 A 409 |
| Chain | Residue |
| A | THR43 |
| A | HIS48 |
| A | ARG89 |
| site_id | AD1 |
| Number of Residues | 16 |
| Details | binding site for residue TYS B 401 |
| Chain | Residue |
| B | TYR37 |
| B | GLY39 |
| B | PHE40 |
| B | ASP41 |
| B | VAL71 |
| B | ASP81 |
| B | ASN126 |
| B | TYR175 |
| B | GLN179 |
| B | GLY182 |
| B | LEU186 |
| B | GLN201 |
| B | HOH528 |
| B | HOH544 |
| B | HOH587 |
| B | HOH601 |
| site_id | AD2 |
| Number of Residues | 3 |
| Details | binding site for residue PG4 B 402 |
| Chain | Residue |
| A | LYS295 |
| B | TRP245 |
| B | LYS250 |
| site_id | AD3 |
| Number of Residues | 11 |
| Details | binding site for residue PG4 B 403 |
| Chain | Residue |
| B | HIS145 |
| B | PHE146 |
| B | SER147 |
| B | GLN150 |
| B | SER207 |
| B | ASP210 |
| B | ARG213 |
| B | ARG214 |
| B | HOH501 |
| B | HOH524 |
| B | HOH579 |
| site_id | AD4 |
| Number of Residues | 6 |
| Details | binding site for residue NA B 404 |
| Chain | Residue |
| B | ILE79 |
| B | ARG89 |
| B | LEU91 |
| B | GLU172 |
| B | HOH535 |
| B | HOH541 |
| site_id | AD5 |
| Number of Residues | 5 |
| Details | binding site for residue NA B 405 |
| Chain | Residue |
| B | ASN5 |
| B | ALA26 |
| B | LEU29 |
| B | ALA30 |
| B | HOH642 |
| site_id | AD6 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 B 406 |
| Chain | Residue |
| B | LYS85 |
| B | ALA86 |
| B | ALA87 |
| B | HOH507 |
| site_id | AD7 |
| Number of Residues | 5 |
| Details | binding site for residue SO4 B 407 |
| Chain | Residue |
| B | TRP202 |
| B | THR206 |
| B | HOH610 |
| B | HOH628 |
| B | LYS154 |
| site_id | AD8 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 B 408 |
| Chain | Residue |
| B | HIS48 |
| B | ARG89 |
| B | HOH504 |
| site_id | AD9 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 B 409 |
| Chain | Residue |
| B | ARG105 |
| B | LYS230 |
| B | GLY233 |
| B | HOH577 |
Functional Information from PROSITE/UniProt
| site_id | PS00178 |
| Number of Residues | 11 |
| Details | AA_TRNA_LIGASE_I Aminoacyl-transfer RNA synthetases class-I signature. Pt.ADsLHLGHL |
| Chain | Residue | Details |
| A | PRO42-LEU52 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 18 |
| Details | Motif: {"description":"'HIGH' region","evidences":[{"source":"HAMAP-Rule","id":"MF_02006","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | Motif: {"description":"'KMSKS' region","evidences":[{"source":"HAMAP-Rule","id":"MF_02006","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_02006","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15663931","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15671170","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_02006","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15663931","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15671170","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20159998","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_02006","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | Site: {"description":"Cross-linked with tRNA by periodate oxidation"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | Site: {"description":"Cross-linked with tRNA by periodate oxidation; predominant"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"18723842","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
