6W20

ClpAP Disengaged State bound to RepA-GFP

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004176	molecular_function	ATP-dependent peptidase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
A	0006979	biological_process	response to oxidative stress
A	0009368	cellular_component	endopeptidase Clp complex
A	0016887	molecular_function	ATP hydrolysis activity
A	0034605	biological_process	cellular response to heat
A	0043335	biological_process	protein unfolding
B	0004176	molecular_function	ATP-dependent peptidase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
B	0006979	biological_process	response to oxidative stress
B	0009368	cellular_component	endopeptidase Clp complex
B	0016887	molecular_function	ATP hydrolysis activity
B	0034605	biological_process	cellular response to heat
B	0043335	biological_process	protein unfolding
C	0004176	molecular_function	ATP-dependent peptidase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
C	0006979	biological_process	response to oxidative stress
C	0009368	cellular_component	endopeptidase Clp complex
C	0016887	molecular_function	ATP hydrolysis activity
C	0034605	biological_process	cellular response to heat
C	0043335	biological_process	protein unfolding
D	0004176	molecular_function	ATP-dependent peptidase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
D	0006979	biological_process	response to oxidative stress
D	0009368	cellular_component	endopeptidase Clp complex
D	0016887	molecular_function	ATP hydrolysis activity
D	0034605	biological_process	cellular response to heat
D	0043335	biological_process	protein unfolding
E	0004176	molecular_function	ATP-dependent peptidase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
E	0006979	biological_process	response to oxidative stress
E	0009368	cellular_component	endopeptidase Clp complex
E	0016887	molecular_function	ATP hydrolysis activity
E	0034605	biological_process	cellular response to heat
E	0043335	biological_process	protein unfolding
F	0004176	molecular_function	ATP-dependent peptidase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
F	0006979	biological_process	response to oxidative stress
F	0009368	cellular_component	endopeptidase Clp complex
F	0016887	molecular_function	ATP hydrolysis activity
F	0034605	biological_process	cellular response to heat
F	0043335	biological_process	protein unfolding
G	0004176	molecular_function	ATP-dependent peptidase activity
G	0004252	molecular_function	serine-type endopeptidase activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006508	biological_process	proteolysis
G	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
G	0008236	molecular_function	serine-type peptidase activity
G	0009266	biological_process	response to temperature stimulus
G	0009314	biological_process	response to radiation
G	0009368	cellular_component	endopeptidase Clp complex
G	0009376	cellular_component	HslUV protease complex
G	0009408	biological_process	response to heat
G	0010498	biological_process	proteasomal protein catabolic process
G	0016020	cellular_component	membrane
G	0042802	molecular_function	identical protein binding
G	0043068	biological_process	positive regulation of programmed cell death
G	0051117	molecular_function	ATPase binding
H	0004176	molecular_function	ATP-dependent peptidase activity
H	0004252	molecular_function	serine-type endopeptidase activity
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006508	biological_process	proteolysis
H	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
H	0008236	molecular_function	serine-type peptidase activity
H	0009266	biological_process	response to temperature stimulus
H	0009314	biological_process	response to radiation
H	0009368	cellular_component	endopeptidase Clp complex
H	0009376	cellular_component	HslUV protease complex
H	0009408	biological_process	response to heat
H	0010498	biological_process	proteasomal protein catabolic process
H	0016020	cellular_component	membrane
H	0042802	molecular_function	identical protein binding
H	0043068	biological_process	positive regulation of programmed cell death
H	0051117	molecular_function	ATPase binding
I	0004176	molecular_function	ATP-dependent peptidase activity
I	0004252	molecular_function	serine-type endopeptidase activity
I	0005515	molecular_function	protein binding
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0006508	biological_process	proteolysis
I	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
I	0008236	molecular_function	serine-type peptidase activity
I	0009266	biological_process	response to temperature stimulus
I	0009314	biological_process	response to radiation
I	0009368	cellular_component	endopeptidase Clp complex
I	0009376	cellular_component	HslUV protease complex
I	0009408	biological_process	response to heat
I	0010498	biological_process	proteasomal protein catabolic process
I	0016020	cellular_component	membrane
I	0042802	molecular_function	identical protein binding
I	0043068	biological_process	positive regulation of programmed cell death
I	0051117	molecular_function	ATPase binding
J	0004176	molecular_function	ATP-dependent peptidase activity
J	0004252	molecular_function	serine-type endopeptidase activity
J	0005515	molecular_function	protein binding
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0006508	biological_process	proteolysis
J	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
J	0008236	molecular_function	serine-type peptidase activity
J	0009266	biological_process	response to temperature stimulus
J	0009314	biological_process	response to radiation
J	0009368	cellular_component	endopeptidase Clp complex
J	0009376	cellular_component	HslUV protease complex
J	0009408	biological_process	response to heat
J	0010498	biological_process	proteasomal protein catabolic process
J	0016020	cellular_component	membrane
J	0042802	molecular_function	identical protein binding
J	0043068	biological_process	positive regulation of programmed cell death
J	0051117	molecular_function	ATPase binding
K	0004176	molecular_function	ATP-dependent peptidase activity
K	0004252	molecular_function	serine-type endopeptidase activity
K	0005515	molecular_function	protein binding
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0006508	biological_process	proteolysis
K	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
K	0008236	molecular_function	serine-type peptidase activity
K	0009266	biological_process	response to temperature stimulus
K	0009314	biological_process	response to radiation
K	0009368	cellular_component	endopeptidase Clp complex
K	0009376	cellular_component	HslUV protease complex
K	0009408	biological_process	response to heat
K	0010498	biological_process	proteasomal protein catabolic process
K	0016020	cellular_component	membrane
K	0042802	molecular_function	identical protein binding
K	0043068	biological_process	positive regulation of programmed cell death
K	0051117	molecular_function	ATPase binding
L	0004176	molecular_function	ATP-dependent peptidase activity
L	0004252	molecular_function	serine-type endopeptidase activity
L	0005515	molecular_function	protein binding
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0006508	biological_process	proteolysis
L	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
L	0008236	molecular_function	serine-type peptidase activity
L	0009266	biological_process	response to temperature stimulus
L	0009314	biological_process	response to radiation
L	0009368	cellular_component	endopeptidase Clp complex
L	0009376	cellular_component	HslUV protease complex
L	0009408	biological_process	response to heat
L	0010498	biological_process	proteasomal protein catabolic process
L	0016020	cellular_component	membrane
L	0042802	molecular_function	identical protein binding
L	0043068	biological_process	positive regulation of programmed cell death
L	0051117	molecular_function	ATPase binding
M	0004176	molecular_function	ATP-dependent peptidase activity
M	0004252	molecular_function	serine-type endopeptidase activity
M	0005515	molecular_function	protein binding
M	0005737	cellular_component	cytoplasm
M	0005829	cellular_component	cytosol
M	0006508	biological_process	proteolysis
M	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
M	0008236	molecular_function	serine-type peptidase activity
M	0009266	biological_process	response to temperature stimulus
M	0009314	biological_process	response to radiation
M	0009368	cellular_component	endopeptidase Clp complex
M	0009376	cellular_component	HslUV protease complex
M	0009408	biological_process	response to heat
M	0010498	biological_process	proteasomal protein catabolic process
M	0016020	cellular_component	membrane
M	0042802	molecular_function	identical protein binding
M	0043068	biological_process	positive regulation of programmed cell death
M	0051117	molecular_function	ATPase binding
N	0004176	molecular_function	ATP-dependent peptidase activity
N	0004252	molecular_function	serine-type endopeptidase activity
N	0005515	molecular_function	protein binding
N	0005737	cellular_component	cytoplasm
N	0005829	cellular_component	cytosol
N	0006508	biological_process	proteolysis
N	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
N	0008236	molecular_function	serine-type peptidase activity
N	0009266	biological_process	response to temperature stimulus
N	0009314	biological_process	response to radiation
N	0009368	cellular_component	endopeptidase Clp complex
N	0009376	cellular_component	HslUV protease complex
N	0009408	biological_process	response to heat
N	0010498	biological_process	proteasomal protein catabolic process
N	0016020	cellular_component	membrane
N	0042802	molecular_function	identical protein binding
N	0043068	biological_process	positive regulation of programmed cell death
N	0051117	molecular_function	ATPase binding
O	0004176	molecular_function	ATP-dependent peptidase activity
O	0004252	molecular_function	serine-type endopeptidase activity
O	0005515	molecular_function	protein binding
O	0005737	cellular_component	cytoplasm
O	0005829	cellular_component	cytosol
O	0006508	biological_process	proteolysis
O	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
O	0008236	molecular_function	serine-type peptidase activity
O	0009266	biological_process	response to temperature stimulus
O	0009314	biological_process	response to radiation
O	0009368	cellular_component	endopeptidase Clp complex
O	0009376	cellular_component	HslUV protease complex
O	0009408	biological_process	response to heat
O	0010498	biological_process	proteasomal protein catabolic process
O	0016020	cellular_component	membrane
O	0042802	molecular_function	identical protein binding
O	0043068	biological_process	positive regulation of programmed cell death
O	0051117	molecular_function	ATPase binding
P	0004176	molecular_function	ATP-dependent peptidase activity
P	0004252	molecular_function	serine-type endopeptidase activity
P	0005515	molecular_function	protein binding
P	0005737	cellular_component	cytoplasm
P	0005829	cellular_component	cytosol
P	0006508	biological_process	proteolysis
P	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
P	0008236	molecular_function	serine-type peptidase activity
P	0009266	biological_process	response to temperature stimulus
P	0009314	biological_process	response to radiation
P	0009368	cellular_component	endopeptidase Clp complex
P	0009376	cellular_component	HslUV protease complex
P	0009408	biological_process	response to heat
P	0010498	biological_process	proteasomal protein catabolic process
P	0016020	cellular_component	membrane
P	0042802	molecular_function	identical protein binding
P	0043068	biological_process	positive regulation of programmed cell death
P	0051117	molecular_function	ATPase binding
Q	0004176	molecular_function	ATP-dependent peptidase activity
Q	0004252	molecular_function	serine-type endopeptidase activity
Q	0005515	molecular_function	protein binding
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0006508	biological_process	proteolysis
Q	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
Q	0008236	molecular_function	serine-type peptidase activity
Q	0009266	biological_process	response to temperature stimulus
Q	0009314	biological_process	response to radiation
Q	0009368	cellular_component	endopeptidase Clp complex
Q	0009376	cellular_component	HslUV protease complex
Q	0009408	biological_process	response to heat
Q	0010498	biological_process	proteasomal protein catabolic process
Q	0016020	cellular_component	membrane
Q	0042802	molecular_function	identical protein binding
Q	0043068	biological_process	positive regulation of programmed cell death
Q	0051117	molecular_function	ATPase binding
R	0004176	molecular_function	ATP-dependent peptidase activity
R	0004252	molecular_function	serine-type endopeptidase activity
R	0005515	molecular_function	protein binding
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0006508	biological_process	proteolysis
R	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
R	0008236	molecular_function	serine-type peptidase activity
R	0009266	biological_process	response to temperature stimulus
R	0009314	biological_process	response to radiation
R	0009368	cellular_component	endopeptidase Clp complex
R	0009376	cellular_component	HslUV protease complex
R	0009408	biological_process	response to heat
R	0010498	biological_process	proteasomal protein catabolic process
R	0016020	cellular_component	membrane
R	0042802	molecular_function	identical protein binding
R	0043068	biological_process	positive regulation of programmed cell death
R	0051117	molecular_function	ATPase binding
S	0004176	molecular_function	ATP-dependent peptidase activity
S	0004252	molecular_function	serine-type endopeptidase activity
S	0005515	molecular_function	protein binding
S	0005737	cellular_component	cytoplasm
S	0005829	cellular_component	cytosol
S	0006508	biological_process	proteolysis
S	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
S	0008236	molecular_function	serine-type peptidase activity
S	0009266	biological_process	response to temperature stimulus
S	0009314	biological_process	response to radiation
S	0009368	cellular_component	endopeptidase Clp complex
S	0009376	cellular_component	HslUV protease complex
S	0009408	biological_process	response to heat
S	0010498	biological_process	proteasomal protein catabolic process
S	0016020	cellular_component	membrane
S	0042802	molecular_function	identical protein binding
S	0043068	biological_process	positive regulation of programmed cell death
S	0051117	molecular_function	ATPase binding
T	0004176	molecular_function	ATP-dependent peptidase activity
T	0004252	molecular_function	serine-type endopeptidase activity
T	0005515	molecular_function	protein binding
T	0005737	cellular_component	cytoplasm
T	0005829	cellular_component	cytosol
T	0006508	biological_process	proteolysis
T	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
T	0008236	molecular_function	serine-type peptidase activity
T	0009266	biological_process	response to temperature stimulus
T	0009314	biological_process	response to radiation
T	0009368	cellular_component	endopeptidase Clp complex
T	0009376	cellular_component	HslUV protease complex
T	0009408	biological_process	response to heat
T	0010498	biological_process	proteasomal protein catabolic process
T	0016020	cellular_component	membrane
T	0042802	molecular_function	identical protein binding
T	0043068	biological_process	positive regulation of programmed cell death
T	0051117	molecular_function	ATPase binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	14
Details	binding site for residue ADP A 801

Chain	Residue
A	ASP186
A	LEU361
A	PRO395
A	ASP396
A	ILE399
F	ARG339
A	PRO187
A	LEU188
A	ILE189
A	GLY217
A	GLY219
A	LYS220
A	THR221
A	ILE357

---

site_id	AC2
Number of Residues	14
Details	binding site for residue ADP A 802

Chain	Residue
A	VAL460
A	PHE461
A	GLN463
A	THR497
A	GLY498
A	VAL499
A	GLY500
A	LYS501
A	THR502
A	GLU503
A	VAL661
A	PHE665
A	ALA701
A	ARG702

---

site_id	AC3
Number of Residues	19
Details	binding site for residue ATP B 801

Chain	Residue
A	ARG339
B	PRO187
B	LEU188
B	ILE189
B	ARG191
B	GLU215
B	SER216
B	GLY217
B	VAL218
B	GLY219
B	LYS220
B	THR221
B	ALA222
B	THR323
B	ILE357
B	LEU361
B	PRO395
B	ASP396
B	ILE399

---

site_id	AC4
Number of Residues	13
Details	binding site for residue ATP B 802

Chain	Residue
B	LEU459
B	VAL460
B	PHE461
B	THR497
B	GLY498
B	VAL499
B	GLY500
B	LYS501
B	THR502
B	GLU503
B	PHE665
B	ALA701
B	ARG702

---

site_id	AC5
Number of Residues	17
Details	binding site for residue ATP C 801

Chain	Residue
B	ALA336
B	ARG339
B	ARG340
C	ASP186
C	LEU188
C	ILE189
C	ARG191
C	GLY217
C	GLY219
C	LYS220
C	THR221
C	ALA222
C	THR323
C	ILE357
C	LEU361
C	PRO395
C	ASP396

---

site_id	AC6
Number of Residues	14
Details	binding site for residue ATP C 802

Chain	Residue
B	ARG643
C	VAL460
C	PHE461
C	PRO496
C	THR497
C	GLY498
C	VAL499
C	GLY500
C	LYS501
C	THR502
C	GLU503
C	VAL661
C	LYS664
C	ARG702

---

site_id	AC7
Number of Residues	18
Details	binding site for residue ATP D 801

Chain	Residue
D	SER216
D	GLY217
D	GLY219
D	LYS220
D	THR221
D	ALA222
D	THR323
D	LEU361
D	PRO395
D	ILE399
C	ALA336
C	ARG339
C	ARG340
D	ASP186
D	PRO187
D	LEU188
D	ILE189
D	ARG191

---

site_id	AC8
Number of Residues	16
Details	binding site for residue ATP D 802

Chain	Residue
C	GLU639
C	ARG643
D	VAL460
D	PHE461
D	THR497
D	GLY498
D	VAL499
D	GLY500
D	LYS501
D	THR502
D	GLU503
D	GLU565
D	ASN606
D	VAL661
D	LYS664
D	ARG702

---

site_id	AC9
Number of Residues	14
Details	binding site for residue ATP E 801

Chain	Residue
D	ARG339
E	LEU188
E	ILE189
E	ARG191
E	SER216
E	GLY217
E	GLY219
E	LYS220
E	THR221
E	ALA222
E	GLU286
E	LEU361
E	LYS364
E	ILE399

---

site_id	AD1
Number of Residues	18
Details	binding site for residue ATP E 802

Chain	Residue
D	ARG643
E	LEU459
E	VAL460
E	PHE461
E	GLN463
E	THR497
E	GLY498
E	VAL499
E	GLY500
E	LYS501
E	THR502
E	GLU503
E	GLU565
E	ASN606
E	VAL661
E	LYS664
E	ALA701
E	ARG702

---

site_id	AD2
Number of Residues	8
Details	binding site for residue ADP F 801

Chain	Residue
F	PHE461
F	THR497
F	GLY498
F	VAL499
F	THR502
F	LEU653
F	VAL657
F	LYS664

---

Functional Information from PROSITE/UniProt

site_id	PS00870
Number of Residues	13
Details	CLPAB_1 Chaperonins clpA/B signature 1. DAANLIKPlLssG

Chain	Residue	Details
A	ASP302-GLY314

---

site_id	PS00871
Number of Residues	19
Details	CLPAB_2 Chaperonins clpA/B signature 2. RFDmSEYmERhTvSRLiGA

Chain	Residue	Details
A	ARG518-ALA536

---

site_id	PS00381
Number of Residues	12
Details	CLP_PROTEASE_SER Endopeptidase Clp serine active site. TicMGqAASMGA

Chain	Residue	Details
G	THR103-ALA114

---

site_id	PS00382
Number of Residues	14
Details	CLP_PROTEASE_HIS Endopeptidase Clp histidine active site. RfclPnsrVMIHQP

Chain	Residue	Details
G	ARG125-PRO138

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	14
Details	ACT_SITE: Nucleophile => ECO:0000305

Chain	Residue	Details
G	SER111
H	SER111
I	SER111
J	SER111
K	SER111
L	SER111
M	SER111
N	SER111
O	SER111
P	SER111
Q	SER111
R	SER111
S	SER111
T	SER111

---

site_id	SWS_FT_FI2
Number of Residues	28
Details	ACT_SITE: ACT_SITE => ECO:0000305

Chain	Residue	Details
G	HIS136
G	ASP185
H	HIS136
H	ASP185
I	HIS136
I	ASP185
J	HIS136
J	ASP185
K	HIS136
K	ASP185
L	HIS136
L	ASP185
M	HIS136
M	ASP185
N	HIS136
N	ASP185
O	HIS136
O	ASP185
P	HIS136
P	ASP185
Q	HIS136
Q	ASP185
R	HIS136
R	ASP185
S	HIS136
S	ASP185
T	HIS136
T	ASP185

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
G	GLY82	electrostatic stabiliser
G	SER111	covalent catalysis, proton shuttle (general acid/base)
G	MET112	electrostatic stabiliser
G	HIS136	proton shuttle (general acid/base)
G	ASP185	electrostatic stabiliser, modifies pKa

---

site_id	MCSA2
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
H	GLY82	electrostatic stabiliser
H	SER111	covalent catalysis, proton shuttle (general acid/base)
H	MET112	electrostatic stabiliser
H	HIS136	proton shuttle (general acid/base)
H	ASP185	electrostatic stabiliser, modifies pKa

---

site_id	MCSA3
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
I	GLY82	electrostatic stabiliser
I	SER111	covalent catalysis, proton shuttle (general acid/base)
I	MET112	electrostatic stabiliser
I	HIS136	proton shuttle (general acid/base)
I	ASP185	electrostatic stabiliser, modifies pKa

---

site_id	MCSA4
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
J	GLY82	electrostatic stabiliser
J	SER111	covalent catalysis, proton shuttle (general acid/base)
J	MET112	electrostatic stabiliser
J	HIS136	proton shuttle (general acid/base)
J	ASP185	electrostatic stabiliser, modifies pKa

---

site_id	MCSA5
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
K	GLY82	electrostatic stabiliser
K	SER111	covalent catalysis, proton shuttle (general acid/base)
K	MET112	electrostatic stabiliser
K	HIS136	proton shuttle (general acid/base)
K	ASP185	electrostatic stabiliser, modifies pKa

---

site_id	MCSA6
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
L	GLY82	electrostatic stabiliser
L	SER111	covalent catalysis, proton shuttle (general acid/base)
L	MET112	electrostatic stabiliser
L	HIS136	proton shuttle (general acid/base)
L	ASP185	electrostatic stabiliser, modifies pKa

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site_id	MCSA7
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
M	GLY82	electrostatic stabiliser
M	SER111	covalent catalysis, proton shuttle (general acid/base)
M	MET112	electrostatic stabiliser
M	HIS136	proton shuttle (general acid/base)
M	ASP185	electrostatic stabiliser, modifies pKa

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site_id	MCSA8
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
N	GLY82	electrostatic stabiliser
N	SER111	covalent catalysis, proton shuttle (general acid/base)
N	MET112	electrostatic stabiliser
N	HIS136	proton shuttle (general acid/base)
N	ASP185	electrostatic stabiliser, modifies pKa

---

site_id	MCSA9
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
O	GLY82	electrostatic stabiliser
O	SER111	covalent catalysis, proton shuttle (general acid/base)
O	MET112	electrostatic stabiliser
O	HIS136	proton shuttle (general acid/base)
O	ASP185	electrostatic stabiliser, modifies pKa

---

site_id	MCSA10
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
P	GLY82	electrostatic stabiliser
P	SER111	covalent catalysis, proton shuttle (general acid/base)
P	MET112	electrostatic stabiliser
P	HIS136	proton shuttle (general acid/base)
P	ASP185	electrostatic stabiliser, modifies pKa

---

site_id	MCSA11
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
Q	GLY82	electrostatic stabiliser
Q	SER111	covalent catalysis, proton shuttle (general acid/base)
Q	MET112	electrostatic stabiliser
Q	HIS136	proton shuttle (general acid/base)
Q	ASP185	electrostatic stabiliser, modifies pKa

---

site_id	MCSA12
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
R	GLY82	electrostatic stabiliser
R	SER111	covalent catalysis, proton shuttle (general acid/base)
R	MET112	electrostatic stabiliser
R	HIS136	proton shuttle (general acid/base)
R	ASP185	electrostatic stabiliser, modifies pKa

---

site_id	MCSA13
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
S	GLY82	electrostatic stabiliser
S	SER111	covalent catalysis, proton shuttle (general acid/base)
S	MET112	electrostatic stabiliser
S	HIS136	proton shuttle (general acid/base)
S	ASP185	electrostatic stabiliser, modifies pKa

---

site_id	MCSA14
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
T	GLY82	electrostatic stabiliser
T	SER111	covalent catalysis, proton shuttle (general acid/base)
T	MET112	electrostatic stabiliser
T	HIS136	proton shuttle (general acid/base)
T	ASP185	electrostatic stabiliser, modifies pKa

---