6VYK

Cryo-EM structure of mechanosensitive channel MscS in PC-18:1 nanodiscs

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005886	cellular_component	plasma membrane
A	0008381	molecular_function	mechanosensitive monoatomic ion channel activity
A	0009992	biological_process	intracellular water homeostasis
A	0016020	cellular_component	membrane
A	0034220	biological_process	monoatomic ion transmembrane transport
A	0042802	molecular_function	identical protein binding
A	0051260	biological_process	protein homooligomerization
A	0055085	biological_process	transmembrane transport
B	0005886	cellular_component	plasma membrane
B	0008381	molecular_function	mechanosensitive monoatomic ion channel activity
B	0009992	biological_process	intracellular water homeostasis
B	0016020	cellular_component	membrane
B	0034220	biological_process	monoatomic ion transmembrane transport
B	0042802	molecular_function	identical protein binding
B	0051260	biological_process	protein homooligomerization
B	0055085	biological_process	transmembrane transport
C	0005886	cellular_component	plasma membrane
C	0008381	molecular_function	mechanosensitive monoatomic ion channel activity
C	0009992	biological_process	intracellular water homeostasis
C	0016020	cellular_component	membrane
C	0034220	biological_process	monoatomic ion transmembrane transport
C	0042802	molecular_function	identical protein binding
C	0051260	biological_process	protein homooligomerization
C	0055085	biological_process	transmembrane transport
D	0005886	cellular_component	plasma membrane
D	0008381	molecular_function	mechanosensitive monoatomic ion channel activity
D	0009992	biological_process	intracellular water homeostasis
D	0016020	cellular_component	membrane
D	0034220	biological_process	monoatomic ion transmembrane transport
D	0042802	molecular_function	identical protein binding
D	0051260	biological_process	protein homooligomerization
D	0055085	biological_process	transmembrane transport
E	0005886	cellular_component	plasma membrane
E	0008381	molecular_function	mechanosensitive monoatomic ion channel activity
E	0009992	biological_process	intracellular water homeostasis
E	0016020	cellular_component	membrane
E	0034220	biological_process	monoatomic ion transmembrane transport
E	0042802	molecular_function	identical protein binding
E	0051260	biological_process	protein homooligomerization
E	0055085	biological_process	transmembrane transport
F	0005886	cellular_component	plasma membrane
F	0008381	molecular_function	mechanosensitive monoatomic ion channel activity
F	0009992	biological_process	intracellular water homeostasis
F	0016020	cellular_component	membrane
F	0034220	biological_process	monoatomic ion transmembrane transport
F	0042802	molecular_function	identical protein binding
F	0051260	biological_process	protein homooligomerization
F	0055085	biological_process	transmembrane transport
G	0005886	cellular_component	plasma membrane
G	0008381	molecular_function	mechanosensitive monoatomic ion channel activity
G	0009992	biological_process	intracellular water homeostasis
G	0016020	cellular_component	membrane
G	0034220	biological_process	monoatomic ion transmembrane transport
G	0042802	molecular_function	identical protein binding
G	0051260	biological_process	protein homooligomerization
G	0055085	biological_process	transmembrane transport

Functional Information from PDB Data

site_id	AC1
Number of Residues	11
Details	binding site for residue PCW A 301

Chain	Residue
A	TYR27
G	ALA36
G	ARG88
A	ASN30
A	ALA79
A	LEU86
A	GLY87
A	ARG88
A	VAL89
A	GLY90
G	ALA33

---

site_id	AC2
Number of Residues	11
Details	binding site for residue PCW B 301

Chain	Residue
A	ALA33
A	ALA36
A	ILE37
A	ARG88
B	TYR27
B	ASN30
B	ILE31
B	ALA79
B	LEU86
B	ARG88
B	VAL89

---

site_id	AC3
Number of Residues	12
Details	binding site for residue PCW C 301

Chain	Residue
C	ALA33
C	ALA36
C	ILE37
C	VAL40
C	ARG88
D	TYR27
D	ASN30
D	LEU86
D	GLY87
D	ARG88
D	VAL89
D	GLY90

---

site_id	AC4
Number of Residues	13
Details	binding site for residue PCW C 302

Chain	Residue
B	ALA33
B	ALA36
B	ARG88
C	TYR27
C	ASN30
C	ILE31
C	ILE38
C	ALA79
C	LEU82
C	GLY87
C	ARG88
C	VAL89
C	GLY90

---

site_id	AC5
Number of Residues	10
Details	binding site for residue PCW D 301

Chain	Residue
D	ALA33
D	ALA36
D	ILE37
D	ARG88
E	TYR27
E	ALA79
E	LEU86
E	VAL89
E	VAL91
E	VAL96

---

site_id	AC6
Number of Residues	10
Details	binding site for residue PCW F 401

Chain	Residue
F	ALA33
F	ALA36
F	ILE37
F	VAL40
F	ARG88
G	TYR27
G	ASN30
G	LEU86
G	VAL89
G	GLY90

---

site_id	AC7
Number of Residues	13
Details	binding site for residue PCW F 402

Chain	Residue
E	ALA33
E	ALA36
E	ILE37
E	ARG88
F	TYR27
F	ASN30
F	ILE31
F	ALA79
F	LEU86
F	GLY87
F	ARG88
F	VAL89
F	GLY90

---

Functional Information from PROSITE/UniProt

site_id	PS01246
Number of Residues	35
Details	UPF0003 Uncharacterized protein family UPF0003 signature. GTVlsVqifsttMrtaDgkiIvIPNgkIIagniiN

Chain	Residue	Details
A	GLY143-ASN177

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	210
Details	TOPO_DOM: Periplasmic => ECO:0000305|PubMed:15919996

Chain	Residue	Details
A	MET1-ASN30
E	VAL89-GLY90
F	MET1-ASN30
F	VAL89-GLY90
G	MET1-ASN30
G	VAL89-GLY90
A	VAL89-GLY90
B	MET1-ASN30
B	VAL89-GLY90
C	MET1-ASN30
C	VAL89-GLY90
D	MET1-ASN30
D	VAL89-GLY90
E	MET1-ASN30

---

site_id	SWS_FT_FI2
Number of Residues	427
Details	TRANSMEM: Helical => ECO:0000269|PubMed:12446901, ECO:0000269|PubMed:26551077

Chain	Residue	Details
A	ILE31-VAL52
D	ILE31-VAL52
D	PHE68-ARG88
D	VAL91-LEU111
E	ILE31-VAL52
E	PHE68-ARG88
E	VAL91-LEU111
F	ILE31-VAL52
F	PHE68-ARG88
F	VAL91-LEU111
G	ILE31-VAL52
A	PHE68-ARG88
G	PHE68-ARG88
G	VAL91-LEU111
A	VAL91-LEU111
B	ILE31-VAL52
B	PHE68-ARG88
B	VAL91-LEU111
C	ILE31-VAL52
C	PHE68-ARG88
C	VAL91-LEU111

---

site_id	SWS_FT_FI3
Number of Residues	1316
Details	TOPO_DOM: Cytoplasmic => ECO:0000305|PubMed:15919996

Chain	Residue	Details
A	ASN53-ASP67
E	GLN112-ALA286
F	ASN53-ASP67
F	GLN112-ALA286
G	ASN53-ASP67
G	GLN112-ALA286
A	GLN112-ALA286
B	ASN53-ASP67
B	GLN112-ALA286
C	ASN53-ASP67
C	GLN112-ALA286
D	ASN53-ASP67
D	GLN112-ALA286
E	ASN53-ASP67

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