6VWP
Crystal structure of E. coli guanosine kinase in complex with ppGpp
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0006166 | biological_process | purine ribonucleoside salvage |
| A | 0008906 | molecular_function | inosine kinase activity |
| A | 0016301 | molecular_function | kinase activity |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016772 | molecular_function | transferase activity, transferring phosphorus-containing groups |
| A | 0032263 | biological_process | GMP salvage |
| A | 0032264 | biological_process | IMP salvage |
| A | 0097216 | molecular_function | guanosine tetraphosphate binding |
| A | 0106366 | molecular_function | guanosine kinase activity |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0005515 | molecular_function | protein binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0006166 | biological_process | purine ribonucleoside salvage |
| B | 0008906 | molecular_function | inosine kinase activity |
| B | 0016301 | molecular_function | kinase activity |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016772 | molecular_function | transferase activity, transferring phosphorus-containing groups |
| B | 0032263 | biological_process | GMP salvage |
| B | 0032264 | biological_process | IMP salvage |
| B | 0097216 | molecular_function | guanosine tetraphosphate binding |
| B | 0106366 | molecular_function | guanosine kinase activity |
| C | 0000166 | molecular_function | nucleotide binding |
| C | 0005515 | molecular_function | protein binding |
| C | 0005524 | molecular_function | ATP binding |
| C | 0006166 | biological_process | purine ribonucleoside salvage |
| C | 0008906 | molecular_function | inosine kinase activity |
| C | 0016301 | molecular_function | kinase activity |
| C | 0016740 | molecular_function | transferase activity |
| C | 0016772 | molecular_function | transferase activity, transferring phosphorus-containing groups |
| C | 0032263 | biological_process | GMP salvage |
| C | 0032264 | biological_process | IMP salvage |
| C | 0097216 | molecular_function | guanosine tetraphosphate binding |
| C | 0106366 | molecular_function | guanosine kinase activity |
| D | 0000166 | molecular_function | nucleotide binding |
| D | 0005515 | molecular_function | protein binding |
| D | 0005524 | molecular_function | ATP binding |
| D | 0006166 | biological_process | purine ribonucleoside salvage |
| D | 0008906 | molecular_function | inosine kinase activity |
| D | 0016301 | molecular_function | kinase activity |
| D | 0016740 | molecular_function | transferase activity |
| D | 0016772 | molecular_function | transferase activity, transferring phosphorus-containing groups |
| D | 0032263 | biological_process | GMP salvage |
| D | 0032264 | biological_process | IMP salvage |
| D | 0097216 | molecular_function | guanosine tetraphosphate binding |
| D | 0106366 | molecular_function | guanosine kinase activity |
| E | 0000166 | molecular_function | nucleotide binding |
| E | 0005515 | molecular_function | protein binding |
| E | 0005524 | molecular_function | ATP binding |
| E | 0006166 | biological_process | purine ribonucleoside salvage |
| E | 0008906 | molecular_function | inosine kinase activity |
| E | 0016301 | molecular_function | kinase activity |
| E | 0016740 | molecular_function | transferase activity |
| E | 0016772 | molecular_function | transferase activity, transferring phosphorus-containing groups |
| E | 0032263 | biological_process | GMP salvage |
| E | 0032264 | biological_process | IMP salvage |
| E | 0097216 | molecular_function | guanosine tetraphosphate binding |
| E | 0106366 | molecular_function | guanosine kinase activity |
| F | 0000166 | molecular_function | nucleotide binding |
| F | 0005515 | molecular_function | protein binding |
| F | 0005524 | molecular_function | ATP binding |
| F | 0006166 | biological_process | purine ribonucleoside salvage |
| F | 0008906 | molecular_function | inosine kinase activity |
| F | 0016301 | molecular_function | kinase activity |
| F | 0016740 | molecular_function | transferase activity |
| F | 0016772 | molecular_function | transferase activity, transferring phosphorus-containing groups |
| F | 0032263 | biological_process | GMP salvage |
| F | 0032264 | biological_process | IMP salvage |
| F | 0097216 | molecular_function | guanosine tetraphosphate binding |
| F | 0106366 | molecular_function | guanosine kinase activity |
| G | 0000166 | molecular_function | nucleotide binding |
| G | 0005515 | molecular_function | protein binding |
| G | 0005524 | molecular_function | ATP binding |
| G | 0006166 | biological_process | purine ribonucleoside salvage |
| G | 0008906 | molecular_function | inosine kinase activity |
| G | 0016301 | molecular_function | kinase activity |
| G | 0016740 | molecular_function | transferase activity |
| G | 0016772 | molecular_function | transferase activity, transferring phosphorus-containing groups |
| G | 0032263 | biological_process | GMP salvage |
| G | 0032264 | biological_process | IMP salvage |
| G | 0097216 | molecular_function | guanosine tetraphosphate binding |
| G | 0106366 | molecular_function | guanosine kinase activity |
| H | 0000166 | molecular_function | nucleotide binding |
| H | 0005515 | molecular_function | protein binding |
| H | 0005524 | molecular_function | ATP binding |
| H | 0006166 | biological_process | purine ribonucleoside salvage |
| H | 0008906 | molecular_function | inosine kinase activity |
| H | 0016301 | molecular_function | kinase activity |
| H | 0016740 | molecular_function | transferase activity |
| H | 0016772 | molecular_function | transferase activity, transferring phosphorus-containing groups |
| H | 0032263 | biological_process | GMP salvage |
| H | 0032264 | biological_process | IMP salvage |
| H | 0097216 | molecular_function | guanosine tetraphosphate binding |
| H | 0106366 | molecular_function | guanosine kinase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 13 |
| Details | binding site for residue G4P A 501 |
| Chain | Residue |
| A | ARG7 |
| A | ALA395 |
| A | GLN396 |
| A | LYS399 |
| B | ARG434 |
| A | SER9 |
| A | LYS10 |
| A | HIS11 |
| A | ARG302 |
| A | PHE321 |
| A | LYS383 |
| A | TYR391 |
| A | SER393 |
| site_id | AC2 |
| Number of Residues | 15 |
| Details | binding site for residue GMP A 502 |
| Chain | Residue |
| A | ASP40 |
| A | GLN41 |
| A | LEU43 |
| A | ASP45 |
| A | SER65 |
| A | GLY93 |
| A | THR94 |
| A | ASN97 |
| A | PHE164 |
| A | ILE166 |
| A | TYR195 |
| A | ARG198 |
| A | ASN354 |
| A | GLY355 |
| A | ASP358 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | binding site for residue GMP A 503 |
| Chain | Residue |
| A | LEU275 |
| A | ASP276 |
| A | VAL278 |
| A | PHE295 |
| A | HIS327 |
| A | ARG335 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | binding site for residue K A 504 |
| Chain | Residue |
| A | LEU244 |
| A | LYS245 |
| A | VAL248 |
| A | TRP277 |
| site_id | AC5 |
| Number of Residues | 2 |
| Details | binding site for residue MG A 505 |
| Chain | Residue |
| A | LEU227 |
| A | GLU257 |
| site_id | AC6 |
| Number of Residues | 14 |
| Details | binding site for residue G4P B 501 |
| Chain | Residue |
| A | ASP433 |
| A | ARG434 |
| B | ARG7 |
| B | SER9 |
| B | LYS10 |
| B | HIS11 |
| B | ARG302 |
| B | PHE321 |
| B | LYS383 |
| B | TYR391 |
| B | SER393 |
| B | ALA395 |
| B | GLN396 |
| B | LYS399 |
| site_id | AC7 |
| Number of Residues | 12 |
| Details | binding site for residue GMP B 502 |
| Chain | Residue |
| B | ASP40 |
| B | GLN41 |
| B | LEU43 |
| B | ASP45 |
| B | SER65 |
| B | GLY93 |
| B | THR94 |
| B | ASN97 |
| B | CYS152 |
| B | ILE166 |
| B | TYR195 |
| B | ARG198 |
| site_id | AC8 |
| Number of Residues | 7 |
| Details | binding site for residue GMP B 503 |
| Chain | Residue |
| B | LEU275 |
| B | ASP276 |
| B | TRP277 |
| B | VAL278 |
| B | HIS327 |
| B | LYS328 |
| B | ARG335 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | binding site for residue K B 504 |
| Chain | Residue |
| A | LEU130 |
| A | THR133 |
| B | LEU130 |
| B | CYS131 |
| B | THR133 |
| site_id | AD1 |
| Number of Residues | 3 |
| Details | binding site for residue K B 505 |
| Chain | Residue |
| B | LYS245 |
| B | VAL248 |
| B | TRP277 |
| site_id | AD2 |
| Number of Residues | 12 |
| Details | binding site for residue G4P C 501 |
| Chain | Residue |
| C | ARG7 |
| C | SER9 |
| C | LYS10 |
| C | HIS11 |
| C | ARG302 |
| C | PHE321 |
| C | LYS383 |
| C | SER393 |
| C | ALA395 |
| C | GLN396 |
| D | ASP433 |
| D | ARG434 |
| site_id | AD3 |
| Number of Residues | 15 |
| Details | binding site for residue GMP C 502 |
| Chain | Residue |
| C | GLY93 |
| C | THR94 |
| C | ASN97 |
| C | CYS152 |
| C | PHE164 |
| C | ILE166 |
| C | TYR195 |
| C | ARG198 |
| C | ASP358 |
| C | ASP40 |
| C | GLN41 |
| C | LEU43 |
| C | ASP45 |
| C | SER65 |
| C | GLY92 |
| site_id | AD4 |
| Number of Residues | 6 |
| Details | binding site for residue GMP C 503 |
| Chain | Residue |
| C | LEU275 |
| C | ASP276 |
| C | TRP277 |
| C | VAL278 |
| C | HIS327 |
| C | ARG335 |
| site_id | AD5 |
| Number of Residues | 6 |
| Details | binding site for residue K C 504 |
| Chain | Residue |
| C | LEU130 |
| C | CYS131 |
| C | THR133 |
| D | LEU130 |
| D | CYS131 |
| D | THR133 |
| site_id | AD6 |
| Number of Residues | 4 |
| Details | binding site for residue K C 505 |
| Chain | Residue |
| C | LEU244 |
| C | LYS245 |
| C | VAL248 |
| C | TRP277 |
| site_id | AD7 |
| Number of Residues | 4 |
| Details | binding site for residue MG C 506 |
| Chain | Residue |
| C | THR226 |
| C | LEU227 |
| C | GLU257 |
| C | ASP358 |
| site_id | AD8 |
| Number of Residues | 13 |
| Details | binding site for residue G4P D 501 |
| Chain | Residue |
| C | ASP433 |
| C | ARG434 |
| D | ARG7 |
| D | SER9 |
| D | LYS10 |
| D | HIS11 |
| D | ARG302 |
| D | PHE321 |
| D | LYS383 |
| D | SER393 |
| D | ALA395 |
| D | GLN396 |
| D | LYS399 |
| site_id | AD9 |
| Number of Residues | 13 |
| Details | binding site for residue GMP D 502 |
| Chain | Residue |
| D | ASP40 |
| D | GLN41 |
| D | LEU43 |
| D | ASP45 |
| D | SER65 |
| D | GLY93 |
| D | THR94 |
| D | ASN97 |
| D | CYS152 |
| D | PHE164 |
| D | TYR195 |
| D | ARG198 |
| D | ASP358 |
| site_id | AE1 |
| Number of Residues | 6 |
| Details | binding site for residue GMP D 503 |
| Chain | Residue |
| D | LEU275 |
| D | ASP276 |
| D | TRP277 |
| D | VAL278 |
| D | HIS327 |
| D | ARG335 |
| site_id | AE2 |
| Number of Residues | 3 |
| Details | binding site for residue K D 504 |
| Chain | Residue |
| D | LYS245 |
| D | VAL248 |
| D | TRP277 |
| site_id | AE3 |
| Number of Residues | 4 |
| Details | binding site for residue MG D 505 |
| Chain | Residue |
| D | THR226 |
| D | LEU227 |
| D | GLU257 |
| D | ASP358 |
| site_id | AE4 |
| Number of Residues | 12 |
| Details | binding site for residue G4P E 501 |
| Chain | Residue |
| E | ARG7 |
| E | SER9 |
| E | LYS10 |
| E | HIS11 |
| E | ARG302 |
| E | PHE321 |
| E | LYS383 |
| E | SER393 |
| E | ALA395 |
| E | GLN396 |
| F | ASP433 |
| F | ARG434 |
| site_id | AE5 |
| Number of Residues | 16 |
| Details | binding site for residue GMP E 502 |
| Chain | Residue |
| E | ASP40 |
| E | GLN41 |
| E | LEU43 |
| E | ASP45 |
| E | SER65 |
| E | GLY93 |
| E | THR94 |
| E | ASN97 |
| E | CYS152 |
| E | PHE164 |
| E | ILE166 |
| E | TYR195 |
| E | ARG198 |
| E | ASN354 |
| E | ASP358 |
| E | PRO414 |
| site_id | AE6 |
| Number of Residues | 5 |
| Details | binding site for residue GMP E 503 |
| Chain | Residue |
| E | LEU275 |
| E | ASP276 |
| E | TRP277 |
| E | VAL278 |
| E | ARG335 |
| site_id | AE7 |
| Number of Residues | 6 |
| Details | binding site for residue K E 504 |
| Chain | Residue |
| E | LEU130 |
| E | CYS131 |
| E | THR133 |
| F | LEU130 |
| F | CYS131 |
| F | THR133 |
| site_id | AE8 |
| Number of Residues | 4 |
| Details | binding site for residue K E 505 |
| Chain | Residue |
| E | LEU244 |
| E | LYS245 |
| E | VAL248 |
| E | TRP277 |
| site_id | AE9 |
| Number of Residues | 3 |
| Details | binding site for residue MG E 506 |
| Chain | Residue |
| E | LEU227 |
| E | GLU257 |
| E | ASP358 |
| site_id | AF1 |
| Number of Residues | 12 |
| Details | binding site for residue G4P F 501 |
| Chain | Residue |
| E | ASP433 |
| E | ARG434 |
| F | ARG7 |
| F | SER9 |
| F | LYS10 |
| F | HIS11 |
| F | ARG302 |
| F | PHE321 |
| F | LYS383 |
| F | SER393 |
| F | ALA395 |
| F | GLN396 |
| site_id | AF2 |
| Number of Residues | 15 |
| Details | binding site for residue GMP F 502 |
| Chain | Residue |
| F | ASP40 |
| F | GLN41 |
| F | LEU43 |
| F | ASP45 |
| F | SER65 |
| F | GLY92 |
| F | GLY93 |
| F | THR94 |
| F | ASN97 |
| F | CYS152 |
| F | ARG162 |
| F | PHE164 |
| F | TYR195 |
| F | ARG198 |
| F | ASP358 |
| site_id | AF3 |
| Number of Residues | 5 |
| Details | binding site for residue GMP F 503 |
| Chain | Residue |
| F | LEU275 |
| F | ASP276 |
| F | VAL278 |
| F | ASP279 |
| F | HIS327 |
| site_id | AF4 |
| Number of Residues | 4 |
| Details | binding site for residue MG F 505 |
| Chain | Residue |
| F | THR226 |
| F | LEU227 |
| F | GLU257 |
| F | ASP358 |
| site_id | AF5 |
| Number of Residues | 13 |
| Details | binding site for residue G4P G 501 |
| Chain | Residue |
| G | ARG7 |
| G | SER9 |
| G | LYS10 |
| G | HIS11 |
| G | ARG302 |
| G | PHE321 |
| G | LYS383 |
| G | SER393 |
| G | ALA395 |
| G | GLN396 |
| G | LYS399 |
| H | ASP433 |
| H | ARG434 |
| site_id | AF6 |
| Number of Residues | 15 |
| Details | binding site for residue GMP G 502 |
| Chain | Residue |
| G | ASP40 |
| G | GLN41 |
| G | LEU43 |
| G | ASP45 |
| G | SER65 |
| G | GLY93 |
| G | THR94 |
| G | ASN97 |
| G | CYS152 |
| G | PHE164 |
| G | ILE166 |
| G | TYR195 |
| G | ARG198 |
| G | ASN354 |
| G | ASP358 |
| site_id | AF7 |
| Number of Residues | 6 |
| Details | binding site for residue GMP G 503 |
| Chain | Residue |
| G | LEU275 |
| G | ASP276 |
| G | VAL278 |
| G | PHE295 |
| G | HIS327 |
| G | ARG335 |
| site_id | AF8 |
| Number of Residues | 2 |
| Details | binding site for residue K G 504 |
| Chain | Residue |
| G | LYS245 |
| G | VAL248 |
| site_id | AF9 |
| Number of Residues | 4 |
| Details | binding site for residue MG G 505 |
| Chain | Residue |
| G | THR226 |
| G | LEU227 |
| G | GLU257 |
| G | ASP358 |
| site_id | AG1 |
| Number of Residues | 13 |
| Details | binding site for residue G4P H 501 |
| Chain | Residue |
| F | SER426 |
| G | ASP433 |
| G | ARG434 |
| H | ARG7 |
| H | SER9 |
| H | LYS10 |
| H | HIS11 |
| H | ARG302 |
| H | GLN305 |
| H | PHE321 |
| H | LYS383 |
| H | GLN396 |
| H | LYS399 |
| site_id | AG2 |
| Number of Residues | 14 |
| Details | binding site for residue GMP H 502 |
| Chain | Residue |
| H | ASP40 |
| H | GLN41 |
| H | LEU43 |
| H | ASP45 |
| H | SER65 |
| H | GLY92 |
| H | GLY93 |
| H | THR94 |
| H | ASN97 |
| H | CYS152 |
| H | ILE166 |
| H | TYR195 |
| H | ARG198 |
| H | ASP358 |
| site_id | AG3 |
| Number of Residues | 6 |
| Details | binding site for residue GMP H 503 |
| Chain | Residue |
| H | LEU275 |
| H | ASP276 |
| H | VAL278 |
| H | ASP279 |
| H | HIS327 |
| H | ARG335 |
| site_id | AG4 |
| Number of Residues | 5 |
| Details | binding site for residue K H 504 |
| Chain | Residue |
| G | LEU130 |
| G | THR133 |
| H | LEU130 |
| H | CYS131 |
| H | THR133 |
| site_id | AG5 |
| Number of Residues | 3 |
| Details | binding site for residue K H 505 |
| Chain | Residue |
| H | LYS245 |
| H | VAL248 |
| H | TRP277 |
| site_id | AG6 |
| Number of Residues | 3 |
| Details | binding site for residue MG H 506 |
| Chain | Residue |
| H | THR226 |
| H | LEU227 |
| H | GLU257 |
Functional Information from PROSITE/UniProt
| site_id | PS00584 |
| Number of Residues | 14 |
| Details | PFKB_KINASES_2 pfkB family of carbohydrate kinases signature 2. NTnGAGDgalAALL |
| Chain | Residue | Details |
| A | ASN352-LEU365 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 136 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_02246","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"32857952","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
