6VWP
Crystal structure of E. coli guanosine kinase in complex with ppGpp
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0005515 | molecular_function | protein binding |
A | 0005524 | molecular_function | ATP binding |
A | 0006166 | biological_process | purine ribonucleoside salvage |
A | 0008906 | molecular_function | inosine kinase activity |
A | 0016301 | molecular_function | kinase activity |
A | 0016310 | biological_process | phosphorylation |
A | 0016772 | molecular_function | transferase activity, transferring phosphorus-containing groups |
A | 0032263 | biological_process | GMP salvage |
A | 0032264 | biological_process | IMP salvage |
A | 0097216 | molecular_function | guanosine tetraphosphate binding |
A | 0106366 | molecular_function | guanosine kinase activity |
B | 0005515 | molecular_function | protein binding |
B | 0005524 | molecular_function | ATP binding |
B | 0006166 | biological_process | purine ribonucleoside salvage |
B | 0008906 | molecular_function | inosine kinase activity |
B | 0016301 | molecular_function | kinase activity |
B | 0016310 | biological_process | phosphorylation |
B | 0016772 | molecular_function | transferase activity, transferring phosphorus-containing groups |
B | 0032263 | biological_process | GMP salvage |
B | 0032264 | biological_process | IMP salvage |
B | 0097216 | molecular_function | guanosine tetraphosphate binding |
B | 0106366 | molecular_function | guanosine kinase activity |
C | 0005515 | molecular_function | protein binding |
C | 0005524 | molecular_function | ATP binding |
C | 0006166 | biological_process | purine ribonucleoside salvage |
C | 0008906 | molecular_function | inosine kinase activity |
C | 0016301 | molecular_function | kinase activity |
C | 0016310 | biological_process | phosphorylation |
C | 0016772 | molecular_function | transferase activity, transferring phosphorus-containing groups |
C | 0032263 | biological_process | GMP salvage |
C | 0032264 | biological_process | IMP salvage |
C | 0097216 | molecular_function | guanosine tetraphosphate binding |
C | 0106366 | molecular_function | guanosine kinase activity |
D | 0005515 | molecular_function | protein binding |
D | 0005524 | molecular_function | ATP binding |
D | 0006166 | biological_process | purine ribonucleoside salvage |
D | 0008906 | molecular_function | inosine kinase activity |
D | 0016301 | molecular_function | kinase activity |
D | 0016310 | biological_process | phosphorylation |
D | 0016772 | molecular_function | transferase activity, transferring phosphorus-containing groups |
D | 0032263 | biological_process | GMP salvage |
D | 0032264 | biological_process | IMP salvage |
D | 0097216 | molecular_function | guanosine tetraphosphate binding |
D | 0106366 | molecular_function | guanosine kinase activity |
E | 0005515 | molecular_function | protein binding |
E | 0005524 | molecular_function | ATP binding |
E | 0006166 | biological_process | purine ribonucleoside salvage |
E | 0008906 | molecular_function | inosine kinase activity |
E | 0016301 | molecular_function | kinase activity |
E | 0016310 | biological_process | phosphorylation |
E | 0016772 | molecular_function | transferase activity, transferring phosphorus-containing groups |
E | 0032263 | biological_process | GMP salvage |
E | 0032264 | biological_process | IMP salvage |
E | 0097216 | molecular_function | guanosine tetraphosphate binding |
E | 0106366 | molecular_function | guanosine kinase activity |
F | 0005515 | molecular_function | protein binding |
F | 0005524 | molecular_function | ATP binding |
F | 0006166 | biological_process | purine ribonucleoside salvage |
F | 0008906 | molecular_function | inosine kinase activity |
F | 0016301 | molecular_function | kinase activity |
F | 0016310 | biological_process | phosphorylation |
F | 0016772 | molecular_function | transferase activity, transferring phosphorus-containing groups |
F | 0032263 | biological_process | GMP salvage |
F | 0032264 | biological_process | IMP salvage |
F | 0097216 | molecular_function | guanosine tetraphosphate binding |
F | 0106366 | molecular_function | guanosine kinase activity |
G | 0005515 | molecular_function | protein binding |
G | 0005524 | molecular_function | ATP binding |
G | 0006166 | biological_process | purine ribonucleoside salvage |
G | 0008906 | molecular_function | inosine kinase activity |
G | 0016301 | molecular_function | kinase activity |
G | 0016310 | biological_process | phosphorylation |
G | 0016772 | molecular_function | transferase activity, transferring phosphorus-containing groups |
G | 0032263 | biological_process | GMP salvage |
G | 0032264 | biological_process | IMP salvage |
G | 0097216 | molecular_function | guanosine tetraphosphate binding |
G | 0106366 | molecular_function | guanosine kinase activity |
H | 0005515 | molecular_function | protein binding |
H | 0005524 | molecular_function | ATP binding |
H | 0006166 | biological_process | purine ribonucleoside salvage |
H | 0008906 | molecular_function | inosine kinase activity |
H | 0016301 | molecular_function | kinase activity |
H | 0016310 | biological_process | phosphorylation |
H | 0016772 | molecular_function | transferase activity, transferring phosphorus-containing groups |
H | 0032263 | biological_process | GMP salvage |
H | 0032264 | biological_process | IMP salvage |
H | 0097216 | molecular_function | guanosine tetraphosphate binding |
H | 0106366 | molecular_function | guanosine kinase activity |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 13 |
Details | binding site for residue G4P A 501 |
Chain | Residue |
A | ARG7 |
A | ALA395 |
A | GLN396 |
A | LYS399 |
B | ARG434 |
A | SER9 |
A | LYS10 |
A | HIS11 |
A | ARG302 |
A | PHE321 |
A | LYS383 |
A | TYR391 |
A | SER393 |
site_id | AC2 |
Number of Residues | 15 |
Details | binding site for residue GMP A 502 |
Chain | Residue |
A | ASP40 |
A | GLN41 |
A | LEU43 |
A | ASP45 |
A | SER65 |
A | GLY93 |
A | THR94 |
A | ASN97 |
A | PHE164 |
A | ILE166 |
A | TYR195 |
A | ARG198 |
A | ASN354 |
A | GLY355 |
A | ASP358 |
site_id | AC3 |
Number of Residues | 6 |
Details | binding site for residue GMP A 503 |
Chain | Residue |
A | LEU275 |
A | ASP276 |
A | VAL278 |
A | PHE295 |
A | HIS327 |
A | ARG335 |
site_id | AC4 |
Number of Residues | 4 |
Details | binding site for residue K A 504 |
Chain | Residue |
A | LEU244 |
A | LYS245 |
A | VAL248 |
A | TRP277 |
site_id | AC5 |
Number of Residues | 2 |
Details | binding site for residue MG A 505 |
Chain | Residue |
A | LEU227 |
A | GLU257 |
site_id | AC6 |
Number of Residues | 14 |
Details | binding site for residue G4P B 501 |
Chain | Residue |
A | ASP433 |
A | ARG434 |
B | ARG7 |
B | SER9 |
B | LYS10 |
B | HIS11 |
B | ARG302 |
B | PHE321 |
B | LYS383 |
B | TYR391 |
B | SER393 |
B | ALA395 |
B | GLN396 |
B | LYS399 |
site_id | AC7 |
Number of Residues | 12 |
Details | binding site for residue GMP B 502 |
Chain | Residue |
B | ASP40 |
B | GLN41 |
B | LEU43 |
B | ASP45 |
B | SER65 |
B | GLY93 |
B | THR94 |
B | ASN97 |
B | CYS152 |
B | ILE166 |
B | TYR195 |
B | ARG198 |
site_id | AC8 |
Number of Residues | 7 |
Details | binding site for residue GMP B 503 |
Chain | Residue |
B | LEU275 |
B | ASP276 |
B | TRP277 |
B | VAL278 |
B | HIS327 |
B | LYS328 |
B | ARG335 |
site_id | AC9 |
Number of Residues | 5 |
Details | binding site for residue K B 504 |
Chain | Residue |
A | LEU130 |
A | THR133 |
B | LEU130 |
B | CYS131 |
B | THR133 |
site_id | AD1 |
Number of Residues | 3 |
Details | binding site for residue K B 505 |
Chain | Residue |
B | LYS245 |
B | VAL248 |
B | TRP277 |
site_id | AD2 |
Number of Residues | 12 |
Details | binding site for residue G4P C 501 |
Chain | Residue |
C | ARG7 |
C | SER9 |
C | LYS10 |
C | HIS11 |
C | ARG302 |
C | PHE321 |
C | LYS383 |
C | SER393 |
C | ALA395 |
C | GLN396 |
D | ASP433 |
D | ARG434 |
site_id | AD3 |
Number of Residues | 15 |
Details | binding site for residue GMP C 502 |
Chain | Residue |
C | GLY93 |
C | THR94 |
C | ASN97 |
C | CYS152 |
C | PHE164 |
C | ILE166 |
C | TYR195 |
C | ARG198 |
C | ASP358 |
C | ASP40 |
C | GLN41 |
C | LEU43 |
C | ASP45 |
C | SER65 |
C | GLY92 |
site_id | AD4 |
Number of Residues | 6 |
Details | binding site for residue GMP C 503 |
Chain | Residue |
C | LEU275 |
C | ASP276 |
C | TRP277 |
C | VAL278 |
C | HIS327 |
C | ARG335 |
site_id | AD5 |
Number of Residues | 6 |
Details | binding site for residue K C 504 |
Chain | Residue |
C | LEU130 |
C | CYS131 |
C | THR133 |
D | LEU130 |
D | CYS131 |
D | THR133 |
site_id | AD6 |
Number of Residues | 4 |
Details | binding site for residue K C 505 |
Chain | Residue |
C | LEU244 |
C | LYS245 |
C | VAL248 |
C | TRP277 |
site_id | AD7 |
Number of Residues | 4 |
Details | binding site for residue MG C 506 |
Chain | Residue |
C | THR226 |
C | LEU227 |
C | GLU257 |
C | ASP358 |
site_id | AD8 |
Number of Residues | 13 |
Details | binding site for residue G4P D 501 |
Chain | Residue |
C | ASP433 |
C | ARG434 |
D | ARG7 |
D | SER9 |
D | LYS10 |
D | HIS11 |
D | ARG302 |
D | PHE321 |
D | LYS383 |
D | SER393 |
D | ALA395 |
D | GLN396 |
D | LYS399 |
site_id | AD9 |
Number of Residues | 13 |
Details | binding site for residue GMP D 502 |
Chain | Residue |
D | ASP40 |
D | GLN41 |
D | LEU43 |
D | ASP45 |
D | SER65 |
D | GLY93 |
D | THR94 |
D | ASN97 |
D | CYS152 |
D | PHE164 |
D | TYR195 |
D | ARG198 |
D | ASP358 |
site_id | AE1 |
Number of Residues | 6 |
Details | binding site for residue GMP D 503 |
Chain | Residue |
D | LEU275 |
D | ASP276 |
D | TRP277 |
D | VAL278 |
D | HIS327 |
D | ARG335 |
site_id | AE2 |
Number of Residues | 3 |
Details | binding site for residue K D 504 |
Chain | Residue |
D | LYS245 |
D | VAL248 |
D | TRP277 |
site_id | AE3 |
Number of Residues | 4 |
Details | binding site for residue MG D 505 |
Chain | Residue |
D | THR226 |
D | LEU227 |
D | GLU257 |
D | ASP358 |
site_id | AE4 |
Number of Residues | 12 |
Details | binding site for residue G4P E 501 |
Chain | Residue |
E | ARG7 |
E | SER9 |
E | LYS10 |
E | HIS11 |
E | ARG302 |
E | PHE321 |
E | LYS383 |
E | SER393 |
E | ALA395 |
E | GLN396 |
F | ASP433 |
F | ARG434 |
site_id | AE5 |
Number of Residues | 16 |
Details | binding site for residue GMP E 502 |
Chain | Residue |
E | ASP40 |
E | GLN41 |
E | LEU43 |
E | ASP45 |
E | SER65 |
E | GLY93 |
E | THR94 |
E | ASN97 |
E | CYS152 |
E | PHE164 |
E | ILE166 |
E | TYR195 |
E | ARG198 |
E | ASN354 |
E | ASP358 |
E | PRO414 |
site_id | AE6 |
Number of Residues | 5 |
Details | binding site for residue GMP E 503 |
Chain | Residue |
E | LEU275 |
E | ASP276 |
E | TRP277 |
E | VAL278 |
E | ARG335 |
site_id | AE7 |
Number of Residues | 6 |
Details | binding site for residue K E 504 |
Chain | Residue |
E | LEU130 |
E | CYS131 |
E | THR133 |
F | LEU130 |
F | CYS131 |
F | THR133 |
site_id | AE8 |
Number of Residues | 4 |
Details | binding site for residue K E 505 |
Chain | Residue |
E | LEU244 |
E | LYS245 |
E | VAL248 |
E | TRP277 |
site_id | AE9 |
Number of Residues | 3 |
Details | binding site for residue MG E 506 |
Chain | Residue |
E | LEU227 |
E | GLU257 |
E | ASP358 |
site_id | AF1 |
Number of Residues | 12 |
Details | binding site for residue G4P F 501 |
Chain | Residue |
E | ASP433 |
E | ARG434 |
F | ARG7 |
F | SER9 |
F | LYS10 |
F | HIS11 |
F | ARG302 |
F | PHE321 |
F | LYS383 |
F | SER393 |
F | ALA395 |
F | GLN396 |
site_id | AF2 |
Number of Residues | 15 |
Details | binding site for residue GMP F 502 |
Chain | Residue |
F | ASP40 |
F | GLN41 |
F | LEU43 |
F | ASP45 |
F | SER65 |
F | GLY92 |
F | GLY93 |
F | THR94 |
F | ASN97 |
F | CYS152 |
F | ARG162 |
F | PHE164 |
F | TYR195 |
F | ARG198 |
F | ASP358 |
site_id | AF3 |
Number of Residues | 5 |
Details | binding site for residue GMP F 503 |
Chain | Residue |
F | LEU275 |
F | ASP276 |
F | VAL278 |
F | ASP279 |
F | HIS327 |
site_id | AF4 |
Number of Residues | 4 |
Details | binding site for residue MG F 505 |
Chain | Residue |
F | THR226 |
F | LEU227 |
F | GLU257 |
F | ASP358 |
site_id | AF5 |
Number of Residues | 13 |
Details | binding site for residue G4P G 501 |
Chain | Residue |
G | ARG7 |
G | SER9 |
G | LYS10 |
G | HIS11 |
G | ARG302 |
G | PHE321 |
G | LYS383 |
G | SER393 |
G | ALA395 |
G | GLN396 |
G | LYS399 |
H | ASP433 |
H | ARG434 |
site_id | AF6 |
Number of Residues | 15 |
Details | binding site for residue GMP G 502 |
Chain | Residue |
G | ASP40 |
G | GLN41 |
G | LEU43 |
G | ASP45 |
G | SER65 |
G | GLY93 |
G | THR94 |
G | ASN97 |
G | CYS152 |
G | PHE164 |
G | ILE166 |
G | TYR195 |
G | ARG198 |
G | ASN354 |
G | ASP358 |
site_id | AF7 |
Number of Residues | 6 |
Details | binding site for residue GMP G 503 |
Chain | Residue |
G | LEU275 |
G | ASP276 |
G | VAL278 |
G | PHE295 |
G | HIS327 |
G | ARG335 |
site_id | AF8 |
Number of Residues | 2 |
Details | binding site for residue K G 504 |
Chain | Residue |
G | LYS245 |
G | VAL248 |
site_id | AF9 |
Number of Residues | 4 |
Details | binding site for residue MG G 505 |
Chain | Residue |
G | THR226 |
G | LEU227 |
G | GLU257 |
G | ASP358 |
site_id | AG1 |
Number of Residues | 13 |
Details | binding site for residue G4P H 501 |
Chain | Residue |
F | SER426 |
G | ASP433 |
G | ARG434 |
H | ARG7 |
H | SER9 |
H | LYS10 |
H | HIS11 |
H | ARG302 |
H | GLN305 |
H | PHE321 |
H | LYS383 |
H | GLN396 |
H | LYS399 |
site_id | AG2 |
Number of Residues | 14 |
Details | binding site for residue GMP H 502 |
Chain | Residue |
H | ASP40 |
H | GLN41 |
H | LEU43 |
H | ASP45 |
H | SER65 |
H | GLY92 |
H | GLY93 |
H | THR94 |
H | ASN97 |
H | CYS152 |
H | ILE166 |
H | TYR195 |
H | ARG198 |
H | ASP358 |
site_id | AG3 |
Number of Residues | 6 |
Details | binding site for residue GMP H 503 |
Chain | Residue |
H | LEU275 |
H | ASP276 |
H | VAL278 |
H | ASP279 |
H | HIS327 |
H | ARG335 |
site_id | AG4 |
Number of Residues | 5 |
Details | binding site for residue K H 504 |
Chain | Residue |
G | LEU130 |
G | THR133 |
H | LEU130 |
H | CYS131 |
H | THR133 |
site_id | AG5 |
Number of Residues | 3 |
Details | binding site for residue K H 505 |
Chain | Residue |
H | LYS245 |
H | VAL248 |
H | TRP277 |
site_id | AG6 |
Number of Residues | 3 |
Details | binding site for residue MG H 506 |
Chain | Residue |
H | THR226 |
H | LEU227 |
H | GLU257 |
Functional Information from PROSITE/UniProt
site_id | PS00584 |
Number of Residues | 14 |
Details | PFKB_KINASES_2 pfkB family of carbohydrate kinases signature 2. NTnGAGDgalAALL |
Chain | Residue | Details |
A | ASN352-LEU365 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 48 |
Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_02246, ECO:0000305|PubMed:32857952 |
Chain | Residue | Details |
A | ASP40 | |
B | THR284 | |
B | GLY357 | |
B | ASN402 | |
C | ASP40 | |
C | GLY93 | |
C | ARG198 | |
C | THR284 | |
C | GLY357 | |
C | ASN402 | |
D | ASP40 | |
A | GLY93 | |
D | GLY93 | |
D | ARG198 | |
D | THR284 | |
D | GLY357 | |
D | ASN402 | |
E | ASP40 | |
E | GLY93 | |
E | ARG198 | |
E | THR284 | |
E | GLY357 | |
A | ARG198 | |
E | ASN402 | |
F | ASP40 | |
F | GLY93 | |
F | ARG198 | |
F | THR284 | |
F | GLY357 | |
F | ASN402 | |
G | ASP40 | |
G | GLY93 | |
G | ARG198 | |
A | THR284 | |
G | THR284 | |
G | GLY357 | |
G | ASN402 | |
H | ASP40 | |
H | GLY93 | |
H | ARG198 | |
H | THR284 | |
H | GLY357 | |
H | ASN402 | |
A | GLY357 | |
A | ASN402 | |
B | ASP40 | |
B | GLY93 | |
B | ARG198 |