6VK6
Crystal Structure of Methylosinus trichosporium OB3b Soluble Methane Monooxygenase Hydroxylase
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004497 | molecular_function | monooxygenase activity |
| A | 0006725 | biological_process | obsolete cellular aromatic compound metabolic process |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0106317 | molecular_function | methane monooxygenase NADH activity |
| A | 0106318 | molecular_function | methane monooxygenase NADPH activity |
| B | 0004497 | molecular_function | monooxygenase activity |
| B | 0006725 | biological_process | obsolete cellular aromatic compound metabolic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016709 | molecular_function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen |
| C | 0004497 | molecular_function | monooxygenase activity |
| C | 0015049 | molecular_function | methane monooxygenase activity |
| C | 0015947 | biological_process | methane metabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 7 |
| Details | binding site for residue FE A 801 |
| Chain | Residue |
| A | GLU114 |
| A | GLU144 |
| A | HIS147 |
| A | FE802 |
| A | EDO812 |
| A | HOH952 |
| A | HOH953 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | binding site for residue FE A 802 |
| Chain | Residue |
| A | GLU243 |
| A | HIS246 |
| A | FE801 |
| A | EDO812 |
| A | HOH953 |
| A | GLU144 |
| A | GLU209 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 803 |
| Chain | Residue |
| A | LYS295 |
| A | LYS297 |
| A | GLU367 |
| A | ASP368 |
| A | HOH1099 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | binding site for residue EDO A 804 |
| Chain | Residue |
| A | VAL62 |
| A | ILE63 |
| A | ALA64 |
| A | ASN135 |
| A | ALA139 |
| A | HOH919 |
| A | HOH1097 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | binding site for residue EDO A 805 |
| Chain | Residue |
| A | ALA210 |
| A | ASN214 |
| A | MET247 |
| A | ASP312 |
| A | TRP313 |
| A | HOH1145 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | binding site for residue EDO A 806 |
| Chain | Residue |
| A | LEU180 |
| A | GLY357 |
| A | PHE358 |
| A | EDO825 |
| A | HOH906 |
| A | HOH1115 |
| site_id | AC7 |
| Number of Residues | 10 |
| Details | binding site for residue EDO A 807 |
| Chain | Residue |
| A | ARG98 |
| A | GLU101 |
| A | THR102 |
| A | TYR288 |
| A | GLY293 |
| A | ALA359 |
| A | LEU361 |
| A | HOH907 |
| A | HOH936 |
| A | HOH1283 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 808 |
| Chain | Residue |
| A | TYR340 |
| A | TRP341 |
| A | TYR376 |
| A | HOH1013 |
| A | HOH1088 |
| site_id | AC9 |
| Number of Residues | 7 |
| Details | binding site for residue EDO A 809 |
| Chain | Residue |
| A | ASP334 |
| A | ARG437 |
| A | ASP450 |
| A | ASP451 |
| A | HOH923 |
| A | HOH1000 |
| A | HOH1027 |
| site_id | AD1 |
| Number of Residues | 7 |
| Details | binding site for residue EDO A 810 |
| Chain | Residue |
| A | HIS382 |
| A | SER428 |
| A | LEU429 |
| A | ALA430 |
| A | HOH904 |
| A | HOH1031 |
| A | HOH1303 |
| site_id | AD2 |
| Number of Residues | 8 |
| Details | binding site for residue EDO A 811 |
| Chain | Residue |
| A | LEU14 |
| A | LYS15 |
| A | VAL16 |
| A | ARG18 |
| A | EDO817 |
| A | HOH964 |
| A | HOH1263 |
| B | ARG137 |
| site_id | AD3 |
| Number of Residues | 11 |
| Details | binding site for residue EDO A 812 |
| Chain | Residue |
| A | GLU114 |
| A | GLU144 |
| A | PHE188 |
| A | GLU209 |
| A | THR213 |
| A | GLU243 |
| A | FE801 |
| A | FE802 |
| A | HOH952 |
| A | HOH953 |
| A | HOH1141 |
| site_id | AD4 |
| Number of Residues | 3 |
| Details | binding site for residue EDO A 813 |
| Chain | Residue |
| A | ASP12 |
| A | LYS15 |
| A | HOH925 |
| site_id | AD5 |
| Number of Residues | 7 |
| Details | binding site for residue EDO A 814 |
| Chain | Residue |
| A | PRO261 |
| A | ALA262 |
| A | LYS265 |
| A | HOH915 |
| A | HOH978 |
| C | GLU142 |
| C | HOH321 |
| site_id | AD6 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 815 |
| Chain | Residue |
| A | GLY412 |
| A | HOH1057 |
| A | ASN375 |
| A | LEU409 |
| A | ALA410 |
| site_id | AD7 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 816 |
| Chain | Residue |
| A | ALA73 |
| A | LYS74 |
| A | HOH916 |
| A | HOH922 |
| A | HOH1291 |
| site_id | AD8 |
| Number of Residues | 10 |
| Details | binding site for residue EDO A 817 |
| Chain | Residue |
| A | LEU14 |
| A | LYS15 |
| A | EDO811 |
| A | HOH901 |
| A | HOH912 |
| B | ARG137 |
| B | GLN362 |
| B | ARG363 |
| B | GLY366 |
| B | ASP367 |
| site_id | AD9 |
| Number of Residues | 3 |
| Details | binding site for residue EDO A 818 |
| Chain | Residue |
| A | LYS385 |
| A | TRP407 |
| A | EDO819 |
| site_id | AE1 |
| Number of Residues | 3 |
| Details | binding site for residue EDO A 819 |
| Chain | Residue |
| A | ILE386 |
| A | EDO818 |
| A | HOH1205 |
| site_id | AE2 |
| Number of Residues | 4 |
| Details | binding site for residue EDO A 820 |
| Chain | Residue |
| A | TYR324 |
| A | GLY325 |
| A | HOH905 |
| C | LYS22 |
| site_id | AE3 |
| Number of Residues | 3 |
| Details | binding site for residue EDO A 821 |
| Chain | Residue |
| A | ASP84 |
| A | LEU237 |
| A | HOH1192 |
| site_id | AE4 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 822 |
| Chain | Residue |
| A | VAL21 |
| A | GLY22 |
| A | VAL23 |
| A | HOH909 |
| A | HOH913 |
| site_id | AE5 |
| Number of Residues | 4 |
| Details | binding site for residue EDO A 823 |
| Chain | Residue |
| A | VAL298 |
| A | TRP371 |
| A | HOH990 |
| A | HOH1216 |
| site_id | AE6 |
| Number of Residues | 4 |
| Details | binding site for residue EDO A 824 |
| Chain | Residue |
| A | SER479 |
| A | HOH920 |
| A | HOH1281 |
| A | HOH1284 |
| site_id | AE7 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 825 |
| Chain | Residue |
| A | PRO179 |
| A | ALA350 |
| A | MET353 |
| A | EDO806 |
| A | HOH906 |
| site_id | AE8 |
| Number of Residues | 1 |
| Details | binding site for residue CL B 401 |
| Chain | Residue |
| B | PRO208 |
| site_id | AE9 |
| Number of Residues | 1 |
| Details | binding site for residue CL B 402 |
| Chain | Residue |
| B | TYR58 |
| site_id | AF1 |
| Number of Residues | 5 |
| Details | binding site for residue EDO B 403 |
| Chain | Residue |
| A | ARG77 |
| A | PHE79 |
| B | GLU51 |
| B | TRP108 |
| B | HOH672 |
| site_id | AF2 |
| Number of Residues | 6 |
| Details | binding site for residue EDO B 404 |
| Chain | Residue |
| B | ASP145 |
| B | ASN149 |
| B | GLY209 |
| B | ASP211 |
| B | HOH590 |
| B | HOH663 |
| site_id | AF3 |
| Number of Residues | 8 |
| Details | binding site for residue EDO B 405 |
| Chain | Residue |
| B | PHE126 |
| B | GLN269 |
| B | THR278 |
| B | PRO281 |
| B | ALA285 |
| B | HOH501 |
| B | HOH504 |
| B | HOH510 |
| site_id | AF4 |
| Number of Residues | 1 |
| Details | binding site for residue EDO B 406 |
| Chain | Residue |
| B | LYS344 |
| site_id | AF5 |
| Number of Residues | 3 |
| Details | binding site for residue EDO B 407 |
| Chain | Residue |
| B | ARG36 |
| B | HOH745 |
| B | HOH769 |
| site_id | AF6 |
| Number of Residues | 3 |
| Details | binding site for residue EDO B 408 |
| Chain | Residue |
| B | ALA232 |
| B | GLU236 |
| B | SER330 |
| site_id | AF7 |
| Number of Residues | 6 |
| Details | binding site for residue EDO B 409 |
| Chain | Residue |
| A | GLY503 |
| B | ALA25 |
| B | ALA26 |
| B | VAL27 |
| B | HOH579 |
| B | HOH656 |
| site_id | AF8 |
| Number of Residues | 4 |
| Details | binding site for residue EDO B 410 |
| Chain | Residue |
| B | ASP309 |
| B | SER310 |
| B | GLY313 |
| B | ALA314 |
| site_id | AF9 |
| Number of Residues | 6 |
| Details | binding site for residue EDO B 411 |
| Chain | Residue |
| B | GLU311 |
| B | HOH693 |
| C | LYS64 |
| C | VAL67 |
| C | EDO208 |
| C | HOH314 |
| site_id | AG1 |
| Number of Residues | 7 |
| Details | binding site for residue EDO B 412 |
| Chain | Residue |
| B | TYR342 |
| B | VAL345 |
| B | LYS347 |
| B | THR352 |
| B | TYR393 |
| B | HOH502 |
| B | HOH604 |
| site_id | AG2 |
| Number of Residues | 5 |
| Details | binding site for residue EDO B 413 |
| Chain | Residue |
| A | PRO20 |
| B | GLN292 |
| B | THR293 |
| B | HOH641 |
| B | HOH689 |
| site_id | AG3 |
| Number of Residues | 7 |
| Details | binding site for residue EDO B 414 |
| Chain | Residue |
| B | ASP64 |
| B | ALA67 |
| B | GLY68 |
| B | GLY69 |
| B | HOH588 |
| C | ARG59 |
| C | HOH464 |
| site_id | AG4 |
| Number of Residues | 1 |
| Details | binding site for residue CL C 202 |
| Chain | Residue |
| C | HOH419 |
| site_id | AG5 |
| Number of Residues | 7 |
| Details | binding site for residue EDO C 203 |
| Chain | Residue |
| C | ILE12 |
| C | ASP49 |
| C | ILE50 |
| C | ASP51 |
| C | VAL52 |
| C | ASP53 |
| C | HOH355 |
| site_id | AG6 |
| Number of Residues | 3 |
| Details | binding site for residue EDO C 204 |
| Chain | Residue |
| C | ASP27 |
| C | THR83 |
| C | GLY85 |
| site_id | AG7 |
| Number of Residues | 5 |
| Details | binding site for residue EDO C 205 |
| Chain | Residue |
| C | ARG157 |
| C | GLN168 |
| C | HOH301 |
| C | HOH303 |
| C | HOH304 |
| site_id | AG8 |
| Number of Residues | 5 |
| Details | binding site for residue EDO C 206 |
| Chain | Residue |
| C | ALA93 |
| C | HOH322 |
| C | HOH336 |
| C | HOH357 |
| C | HOH364 |
| site_id | AG9 |
| Number of Residues | 2 |
| Details | binding site for residue EDO C 207 |
| Chain | Residue |
| C | GLN34 |
| C | ASP35 |
| site_id | AH1 |
| Number of Residues | 5 |
| Details | binding site for residue EDO C 208 |
| Chain | Residue |
| B | EDO411 |
| C | LYS64 |
| C | VAL67 |
| C | LEU68 |
| C | GLU71 |
| site_id | AH2 |
| Number of Residues | 4 |
| Details | binding site for residue EDO C 209 |
| Chain | Residue |
| A | PRO427 |
| A | HOH943 |
| C | HIS166 |
| C | GLN168 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: ACT_SITE => ECO:0000255 |
| Chain | Residue | Details |
| A | CYS151 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000250 |
| Chain | Residue | Details |
| A | GLU114 | |
| A | GLU144 | |
| A | HIS147 | |
| A | GLU209 | |
| A | GLU243 | |
| A | HIS246 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 600 |
| Chain | Residue | Details |
| A | GLU114 | metal ligand |
| A | GLU144 | metal ligand |
| A | HIS147 | metal ligand |
| A | GLU209 | metal ligand |
| A | GLU243 | hydrogen bond donor, metal ligand |
| A | HIS246 | metal ligand |
