Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6VK5

Crystal Structure of Methylosinus trichosporium OB3b Soluble Methane Monooxygenase Hydroxylase and Regulatory Component Complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004497	molecular_function	monooxygenase activity
A	0006730	biological_process	one-carbon metabolic process
A	0015049	molecular_function	methane monooxygenase [NAD(P)H] activity
A	0016491	molecular_function	oxidoreductase activity
A	0046872	molecular_function	metal ion binding
B	0004497	molecular_function	monooxygenase activity
B	0016491	molecular_function	oxidoreductase activity
B	0016709	molecular_function	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
C	0004497	molecular_function	monooxygenase activity
C	0015049	molecular_function	methane monooxygenase [NAD(P)H] activity
C	0015947	biological_process	methane metabolic process
D	0004497	molecular_function	monooxygenase activity
D	0016491	molecular_function	oxidoreductase activity
E	0004497	molecular_function	monooxygenase activity
E	0006730	biological_process	one-carbon metabolic process
E	0015049	molecular_function	methane monooxygenase [NAD(P)H] activity
E	0016491	molecular_function	oxidoreductase activity
E	0046872	molecular_function	metal ion binding
F	0004497	molecular_function	monooxygenase activity
F	0016491	molecular_function	oxidoreductase activity
F	0016709	molecular_function	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
G	0004497	molecular_function	monooxygenase activity
G	0015049	molecular_function	methane monooxygenase [NAD(P)H] activity
G	0015947	biological_process	methane metabolic process
H	0004497	molecular_function	monooxygenase activity
H	0016491	molecular_function	oxidoreductase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	binding site for residue FE A 801

Chain	Residue
A	GLU114
A	GLU144
A	HIS147
A	FE802
A	BEZ813
A	HOH907
A	HOH912

site_id	AC2
Number of Residues	7
Details	binding site for residue FE A 802

Chain	Residue
A	GLU243
A	HIS246
A	FE801
A	BEZ813
A	HOH912
A	GLU144
A	GLU209

site_id	AC3
Number of Residues	2
Details	binding site for residue EDO A 803

Chain	Residue
A	TRP341
A	HOH941

site_id	AC4
Number of Residues	5
Details	binding site for residue EDO A 804

Chain	Residue
A	GLN406
A	HOH1043
A	HOH1154
B	ASN308
B	HOH538

site_id	AC5
Number of Residues	8
Details	binding site for residue EDO A 805

Chain	Residue
A	ARG98
A	THR102
A	TYR288
A	LEU289
A	GLY293
A	LEU361
A	HOH910
A	HOH950

site_id	AC6
Number of Residues	5
Details	binding site for residue EDO A 806

Chain	Residue
A	ASP521
A	HOH913
A	HOH931
A	HOH1128
B	LYS47

site_id	AC7
Number of Residues	9
Details	binding site for residue EDO A 807

Chain	Residue
A	VAL105
A	LEU180
A	GLY357
A	PHE358
A	ALA359
A	EDO808
A	HOH910
A	HOH953
A	HOH1090

site_id	AC8
Number of Residues	8
Details	binding site for residue EDO A 808

Chain	Residue
A	PRO179
A	ALA349
A	ALA350
A	MET353
A	TRP354
A	EDO807
A	HOH953
A	HOH1090

site_id	AC9
Number of Residues	3
Details	binding site for residue EDO A 809

Chain	Residue
A	ASP334
A	ARG337
A	THR433

site_id	AD1
Number of Residues	7
Details	binding site for residue EDO A 810

Chain	Residue
A	ASN268
A	ASN272
A	GLU327
A	SER328
A	ALA330
A	HOH902
A	HOH1137

site_id	AD2
Number of Residues	7
Details	binding site for residue EDO A 811

Chain	Residue
A	GLY191
A	ASN203
A	ALA274
A	THR277
A	HOH926
A	HOH991
B	GLN77

site_id	AD3
Number of Residues	4
Details	binding site for residue EDO A 812

Chain	Residue
A	ASP451
A	ARG455
A	HOH1120
C	TYR149

site_id	AD4
Number of Residues	10
Details	binding site for residue BEZ A 813

Chain	Residue
A	GLU114
A	GLU144
A	PHE188
A	GLU209
A	THR213
A	GLU243
A	FE801
A	FE802
A	HOH907
A	HOH912

site_id	AD5
Number of Residues	4
Details	binding site for residue EDO B 401

Chain	Residue
A	LYS385
B	SER310
B	GLU311
B	HOH509

site_id	AD6
Number of Residues	4
Details	binding site for residue EDO B 402

Chain	Residue
B	GLU51
B	TRP108
B	HOH506
H	LYS97

site_id	AD7
Number of Residues	6
Details	binding site for residue EDO B 403

Chain	Residue
B	ALA257
B	VAL364
B	TRP368
B	ASP371
B	HOH526
B	HOH655

site_id	AD8
Number of Residues	7
Details	binding site for residue EDO C 201

Chain	Residue
C	ASP51
C	VAL52
C	ASP53
C	HOH375
C	ILE12
C	ASP49
C	ILE50

site_id	AD9
Number of Residues	5
Details	binding site for residue EDO D 201

Chain	Residue
D	GLU47
D	PHE99
D	ASP103
D	ILE106
D	ASN107

site_id	AE1
Number of Residues	5
Details	binding site for residue EDO D 202

Chain	Residue
B	ARG12
D	LYS44
D	HOH301
D	HOH307
E	ARG88

site_id	AE2
Number of Residues	7
Details	binding site for residue FE E 801

Chain	Residue
E	GLU114
E	GLU144
E	HIS147
E	FE802
E	BEZ810
E	HOH907
E	HOH912

site_id	AE3
Number of Residues	7
Details	binding site for residue FE E 802

Chain	Residue
E	GLU144
E	GLU209
E	GLU243
E	HIS246
E	FE801
E	BEZ810
E	HOH912

site_id	AE4
Number of Residues	5
Details	binding site for residue EDO E 803

Chain	Residue
E	ARG489
E	SER490
E	HOH941
E	HOH943
E	HOH1122

site_id	AE5
Number of Residues	5
Details	binding site for residue EDO E 804

Chain	Residue
B	ASP367
E	LYS15
E	VAL16
E	ARG18
F	ARG137

site_id	AE6
Number of Residues	2
Details	binding site for residue EDO E 805

Chain	Residue
E	PRO520
E	HOH916

site_id	AE7
Number of Residues	8
Details	binding site for residue EDO E 806

Chain	Residue
E	ASN273
E	GLN456
E	GLU460
E	ARG463
E	TYR464
E	EDO807
E	HOH1039
G	TYR148

site_id	AE8
Number of Residues	9
Details	binding site for residue EDO E 807

Chain	Residue
E	ARG186
E	GLY191
E	ASN203
E	ALA274
E	THR277
E	EDO806
E	HOH902
E	HOH1115
F	GLN77

site_id	AE9
Number of Residues	4
Details	binding site for residue EDO E 808

Chain	Residue
E	ASP451
E	TRP452
E	ARG455
E	HOH1131

site_id	AF1
Number of Residues	4
Details	binding site for residue EDO E 809

Chain	Residue
E	TRP37
E	ASP38
E	HOH959
F	THR222

site_id	AF2
Number of Residues	10
Details	binding site for residue BEZ E 810

Chain	Residue
E	GLU114
E	GLU144
E	PHE188
E	GLU209
E	THR213
E	GLU243
E	FE801
E	FE802
E	HOH907
E	HOH912

site_id	AF3
Number of Residues	1
Details	binding site for residue CL E 811

Chain	Residue
E	LYS30

site_id	AF4
Number of Residues	6
Details	binding site for residue EDO F 401

Chain	Residue
B	PHE126
B	THR278
F	GLN269
F	PRO281
F	ALA285
F	HOH580

site_id	AF5
Number of Residues	3
Details	binding site for residue EDO F 402

Chain	Residue
D	LYS97
F	GLU51
F	HOH530

site_id	AF6
Number of Residues	2
Details	binding site for residue EDO F 403

Chain	Residue
F	LYS150
F	TYR151

site_id	AF7
Number of Residues	3
Details	binding site for residue EDO F 404

Chain	Residue
F	TYR142
F	LYS344
F	GLU346

site_id	AF8
Number of Residues	7
Details	binding site for residue EDO G 201

Chain	Residue
G	ILE12
G	ASP49
G	ILE50
G	ASP51
G	VAL52
G	ASP53
G	HOH320

site_id	AF9
Number of Residues	3
Details	binding site for residue EDO G 202

Chain	Residue
G	VAL26
G	ASP27
G	HOH347

site_id	AG1
Number of Residues	6
Details	binding site for residue EDO H 201

Chain	Residue
H	GLU47
H	PHE99
H	ASP103
H	LEU104
H	ILE106
H	ASN107

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	2
Details	Active site: {"evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 600

Chain	Residue	Details
A	GLU114	metal ligand
A	GLU144	metal ligand
A	HIS147	metal ligand
A	GLU209	metal ligand
A	GLU243	hydrogen bond donor, metal ligand
A	HIS246	metal ligand

site_id	MCSA2
Number of Residues	6
Details	M-CSA 600

Chain	Residue	Details
E	GLU114	metal ligand
E	GLU144	metal ligand
E	HIS147	metal ligand
E	GLU209	metal ligand
E	GLU243	hydrogen bond donor, metal ligand
E	HIS246	metal ligand

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)