6V9W

Structure of TRPA1 (ligand-free) with bound calcium, LMNG

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005216	molecular_function	monoatomic ion channel activity
A	0005262	molecular_function	calcium channel activity
A	0005886	cellular_component	plasma membrane
A	0006811	biological_process	monoatomic ion transport
A	0006816	biological_process	calcium ion transport
A	0006874	biological_process	intracellular calcium ion homeostasis
A	0007166	biological_process	cell surface receptor signaling pathway
A	0009409	biological_process	response to cold
A	0009410	biological_process	response to xenobiotic stimulus
A	0010033	biological_process	response to organic substance
A	0014070	biological_process	response to organic cyclic compound
A	0015267	molecular_function	channel activity
A	0015278	molecular_function	intracellularly gated calcium channel activity
A	0016020	cellular_component	membrane
A	0019233	biological_process	sensory perception of pain
A	0019722	biological_process	calcium-mediated signaling
A	0032421	cellular_component	stereocilium bundle
A	0034220	biological_process	monoatomic ion transmembrane transport
A	0042542	biological_process	response to hydrogen peroxide
A	0042802	molecular_function	identical protein binding
A	0048265	biological_process	response to pain
A	0050955	biological_process	thermoception
A	0050966	biological_process	detection of mechanical stimulus involved in sensory perception of pain
A	0050968	biological_process	detection of chemical stimulus involved in sensory perception of pain
A	0050974	biological_process	detection of mechanical stimulus involved in sensory perception
A	0051289	biological_process	protein homotetramerization
A	0055085	biological_process	transmembrane transport
A	0070301	biological_process	cellular response to hydrogen peroxide
A	0070588	biological_process	calcium ion transmembrane transport
A	0071310	biological_process	cellular response to organic substance
A	0097604	molecular_function	temperature-gated cation channel activity
B	0005216	molecular_function	monoatomic ion channel activity
B	0005262	molecular_function	calcium channel activity
B	0005886	cellular_component	plasma membrane
B	0006811	biological_process	monoatomic ion transport
B	0006816	biological_process	calcium ion transport
B	0006874	biological_process	intracellular calcium ion homeostasis
B	0007166	biological_process	cell surface receptor signaling pathway
B	0009409	biological_process	response to cold
B	0009410	biological_process	response to xenobiotic stimulus
B	0010033	biological_process	response to organic substance
B	0014070	biological_process	response to organic cyclic compound
B	0015267	molecular_function	channel activity
B	0015278	molecular_function	intracellularly gated calcium channel activity
B	0016020	cellular_component	membrane
B	0019233	biological_process	sensory perception of pain
B	0019722	biological_process	calcium-mediated signaling
B	0032421	cellular_component	stereocilium bundle
B	0034220	biological_process	monoatomic ion transmembrane transport
B	0042542	biological_process	response to hydrogen peroxide
B	0042802	molecular_function	identical protein binding
B	0048265	biological_process	response to pain
B	0050955	biological_process	thermoception
B	0050966	biological_process	detection of mechanical stimulus involved in sensory perception of pain
B	0050968	biological_process	detection of chemical stimulus involved in sensory perception of pain
B	0050974	biological_process	detection of mechanical stimulus involved in sensory perception
B	0051289	biological_process	protein homotetramerization
B	0055085	biological_process	transmembrane transport
B	0070301	biological_process	cellular response to hydrogen peroxide
B	0070588	biological_process	calcium ion transmembrane transport
B	0071310	biological_process	cellular response to organic substance
B	0097604	molecular_function	temperature-gated cation channel activity
C	0005216	molecular_function	monoatomic ion channel activity
C	0005262	molecular_function	calcium channel activity
C	0005886	cellular_component	plasma membrane
C	0006811	biological_process	monoatomic ion transport
C	0006816	biological_process	calcium ion transport
C	0006874	biological_process	intracellular calcium ion homeostasis
C	0007166	biological_process	cell surface receptor signaling pathway
C	0009409	biological_process	response to cold
C	0009410	biological_process	response to xenobiotic stimulus
C	0010033	biological_process	response to organic substance
C	0014070	biological_process	response to organic cyclic compound
C	0015267	molecular_function	channel activity
C	0015278	molecular_function	intracellularly gated calcium channel activity
C	0016020	cellular_component	membrane
C	0019233	biological_process	sensory perception of pain
C	0019722	biological_process	calcium-mediated signaling
C	0032421	cellular_component	stereocilium bundle
C	0034220	biological_process	monoatomic ion transmembrane transport
C	0042542	biological_process	response to hydrogen peroxide
C	0042802	molecular_function	identical protein binding
C	0048265	biological_process	response to pain
C	0050955	biological_process	thermoception
C	0050966	biological_process	detection of mechanical stimulus involved in sensory perception of pain
C	0050968	biological_process	detection of chemical stimulus involved in sensory perception of pain
C	0050974	biological_process	detection of mechanical stimulus involved in sensory perception
C	0051289	biological_process	protein homotetramerization
C	0055085	biological_process	transmembrane transport
C	0070301	biological_process	cellular response to hydrogen peroxide
C	0070588	biological_process	calcium ion transmembrane transport
C	0071310	biological_process	cellular response to organic substance
C	0097604	molecular_function	temperature-gated cation channel activity
D	0005216	molecular_function	monoatomic ion channel activity
D	0005262	molecular_function	calcium channel activity
D	0005886	cellular_component	plasma membrane
D	0006811	biological_process	monoatomic ion transport
D	0006816	biological_process	calcium ion transport
D	0006874	biological_process	intracellular calcium ion homeostasis
D	0007166	biological_process	cell surface receptor signaling pathway
D	0009409	biological_process	response to cold
D	0009410	biological_process	response to xenobiotic stimulus
D	0010033	biological_process	response to organic substance
D	0014070	biological_process	response to organic cyclic compound
D	0015267	molecular_function	channel activity
D	0015278	molecular_function	intracellularly gated calcium channel activity
D	0016020	cellular_component	membrane
D	0019233	biological_process	sensory perception of pain
D	0019722	biological_process	calcium-mediated signaling
D	0032421	cellular_component	stereocilium bundle
D	0034220	biological_process	monoatomic ion transmembrane transport
D	0042542	biological_process	response to hydrogen peroxide
D	0042802	molecular_function	identical protein binding
D	0048265	biological_process	response to pain
D	0050955	biological_process	thermoception
D	0050966	biological_process	detection of mechanical stimulus involved in sensory perception of pain
D	0050968	biological_process	detection of chemical stimulus involved in sensory perception of pain
D	0050974	biological_process	detection of mechanical stimulus involved in sensory perception
D	0051289	biological_process	protein homotetramerization
D	0055085	biological_process	transmembrane transport
D	0070301	biological_process	cellular response to hydrogen peroxide
D	0070588	biological_process	calcium ion transmembrane transport
D	0071310	biological_process	cellular response to organic substance
D	0097604	molecular_function	temperature-gated cation channel activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue CA A 1201

Chain	Residue
A	GLU788
A	GLN791
A	ASN805
A	GLU808

---

site_id	AC2
Number of Residues	4
Details	binding site for residue CA B 1201

Chain	Residue
B	GLU788
B	GLN791
B	ASN805
B	GLU808

---

site_id	AC3
Number of Residues	4
Details	binding site for residue CA D 1201

Chain	Residue
D	GLN791
D	ASN805
D	GLU808
D	GLU788

---

site_id	AC4
Number of Residues	4
Details	binding site for residue CA C 1201

Chain	Residue
C	GLU788
C	GLN791
C	ASN805
C	GLU808

---

Functional Information from PROSITE/UniProt

site_id	PS00018
Number of Residues	13
Details	EF_HAND_1 EF-hand calcium-binding domain. DISDTRLLNegDL

Chain	Residue	Details
A	ASP468-LEU480

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3676
Details	TOPO_DOM: Cytoplasmic => ECO:0000305|PubMed:25855297

Chain	Residue	Details
A	MET1-HIS719
D	CYS786-ILE803
D	GLN851-SER873
D	ILE957-PRO1119
C	MET1-HIS719
C	CYS786-ILE803
C	GLN851-SER873
C	ILE957-PRO1119
A	CYS786-ILE803
A	GLN851-SER873
A	ILE957-PRO1119
B	MET1-HIS719
B	CYS786-ILE803
B	GLN851-SER873
B	ILE957-PRO1119
D	MET1-HIS719

---

site_id	SWS_FT_FI2
Number of Residues	80
Details	TRANSMEM: Helical; Name=1 => ECO:0000305|PubMed:25855297

Chain	Residue	Details
A	MET720-ILE740
B	MET720-ILE740
D	MET720-ILE740
C	MET720-ILE740

---

site_id	SWS_FT_FI3
Number of Residues	176
Details	TOPO_DOM: Extracellular => ECO:0000305|PubMed:25855297

Chain	Residue	Details
A	LYS741-THR764
D	GLU825-HIS829
D	GLN895-PRO901
D	LEU923-PRO934
C	LYS741-THR764
C	GLU825-HIS829
C	GLN895-PRO901
C	LEU923-PRO934
A	GLU825-HIS829
A	GLN895-PRO901
A	LEU923-PRO934
B	LYS741-THR764
B	GLU825-HIS829
B	GLN895-PRO901
B	LEU923-PRO934
D	LYS741-THR764

---

site_id	SWS_FT_FI4
Number of Residues	80
Details	TRANSMEM: Helical; Name=2 => ECO:0000305|PubMed:25855297

Chain	Residue	Details
A	THR765-TYR785
B	THR765-TYR785
D	THR765-TYR785
C	THR765-TYR785

---

site_id	SWS_FT_FI5
Number of Residues	80
Details	TRANSMEM: Helical; Name=3 => ECO:0000305|PubMed:25855297

Chain	Residue	Details
A	SER804-VAL824
B	SER804-VAL824
D	SER804-VAL824
C	SER804-VAL824

---

site_id	SWS_FT_FI6
Number of Residues	80
Details	TRANSMEM: Helical; Name=4 => ECO:0000305|PubMed:25855297

Chain	Residue	Details
A	LEU830-LEU850
B	LEU830-LEU850
D	LEU830-LEU850
C	LEU830-LEU850

---

site_id	SWS_FT_FI7
Number of Residues	80
Details	TRANSMEM: Helical; Name=5 => ECO:0000305|PubMed:25855297

Chain	Residue	Details
A	THR874-LEU894
B	THR874-LEU894
D	THR874-LEU894
C	THR874-LEU894

---

site_id	SWS_FT_FI8
Number of Residues	80
Details	INTRAMEM: Pore-forming => ECO:0000305|PubMed:25855297

Chain	Residue	Details
A	LEU902-PHE922
B	LEU902-PHE922
D	LEU902-PHE922
C	LEU902-PHE922

---

site_id	SWS_FT_FI9
Number of Residues	84
Details	TRANSMEM: Helical; Name=6 => ECO:0000305|PubMed:25855297

Chain	Residue	Details
A	VAL935-LEU956
B	VAL935-LEU956
D	VAL935-LEU956
C	VAL935-LEU956

---

site_id	SWS_FT_FI10
Number of Residues	8
Details	BINDING: covalent => ECO:0000250|UniProtKB:Q8BLA8

Chain	Residue	Details
A	CYS414
A	CYS421
B	CYS414
B	CYS421
D	CYS414
D	CYS421
C	CYS414
C	CYS421

---

site_id	SWS_FT_FI11
Number of Residues	4
Details	BINDING: covalent; Cys highly reactive => ECO:0000250|UniProtKB:Q8BLA8, ECO:0000305|PubMed:17164327, ECO:0000305|PubMed:27241698

Chain	Residue	Details
A	CYS621
B	CYS621
D	CYS621
C	CYS621

---

site_id	SWS_FT_FI12
Number of Residues	8
Details	BINDING: covalent => ECO:0000305|PubMed:17164327

Chain	Residue	Details
A	CYS641
A	LYS710
B	CYS641
B	LYS710
D	CYS641
D	LYS710
C	CYS641
C	LYS710

---

site_id	SWS_FT_FI13
Number of Residues	4
Details	BINDING: covalent => ECO:0000305|PubMed:17164327, ECO:0000305|PubMed:27241698

Chain	Residue	Details
A	CYS665
B	CYS665
D	CYS665
C	CYS665

---

site_id	SWS_FT_FI14
Number of Residues	4
Details	BINDING: BINDING => ECO:0000305|PubMed:25855297

Chain	Residue	Details
A	LYS1046
B	LYS1046
D	LYS1046
C	LYS1046

---

site_id	SWS_FT_FI15
Number of Residues	4
Details	SITE: Required for C-621 reactivity => ECO:0000269|PubMed:27241698

Chain	Residue	Details
A	LYS620
B	LYS620
D	LYS620
C	LYS620

---

site_id	SWS_FT_FI16
Number of Residues	4
Details	SITE: Key residue for activation by the scorpion wasabi receptor toxin => ECO:0000269|PubMed:31447178

Chain	Residue	Details
A	PRO622
B	PRO622
D	PRO622
C	PRO622

---

site_id	SWS_FT_FI17
Number of Residues	8
Details	SITE: Important residue for activation by the scorpion wasabi receptor toxin => ECO:0000269|PubMed:31447178

Chain	Residue	Details
A	MET634
A	THR646
B	MET634
B	THR646
D	MET634
D	THR646
C	MET634
C	THR646

---

site_id	SWS_FT_FI18
Number of Residues	4
Details	MOD_RES: 4-hydroxyproline; by EGLN1; transient; in normoxia and hyperoxia => ECO:0000269|PubMed:21873995

Chain	Residue	Details
A	PRO394
B	PRO394
D	PRO394
C	PRO394

---

site_id	SWS_FT_FI19
Number of Residues	8
Details	MOD_RES: Cysteine sulfenic acid (-SOH); transient; in hyperoxia => ECO:0000305|PubMed:21873995

Chain	Residue	Details
A	CYS633
A	CYS856
B	CYS633
B	CYS856
D	CYS633
D	CYS856
C	CYS633
C	CYS856

---

site_id	SWS_FT_FI20
Number of Residues	8
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255

Chain	Residue	Details
A	ASN747
A	ASN753
B	ASN747
B	ASN753
D	ASN747
D	ASN753
C	ASN747
C	ASN753

---