6UZE
Anthrax toxin protective antigen channels bound to edema factor
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005515 | molecular_function | protein binding |
| A | 0005576 | cellular_component | extracellular region |
| A | 0016020 | cellular_component | membrane |
| A | 0020002 | cellular_component | host cell plasma membrane |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0043409 | biological_process | negative regulation of MAPK cascade |
| A | 0044164 | cellular_component | host cell cytosol |
| A | 0044174 | cellular_component | host cell endosome |
| A | 0044175 | cellular_component | host cell endosome membrane |
| A | 0044533 | biological_process | positive regulation of apoptotic process in another organism |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0051260 | biological_process | protein homooligomerization |
| A | 0090729 | molecular_function | toxin activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005576 | cellular_component | extracellular region |
| B | 0016020 | cellular_component | membrane |
| B | 0020002 | cellular_component | host cell plasma membrane |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0043409 | biological_process | negative regulation of MAPK cascade |
| B | 0044164 | cellular_component | host cell cytosol |
| B | 0044174 | cellular_component | host cell endosome |
| B | 0044175 | cellular_component | host cell endosome membrane |
| B | 0044533 | biological_process | positive regulation of apoptotic process in another organism |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0051260 | biological_process | protein homooligomerization |
| B | 0090729 | molecular_function | toxin activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005576 | cellular_component | extracellular region |
| C | 0016020 | cellular_component | membrane |
| C | 0020002 | cellular_component | host cell plasma membrane |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0043409 | biological_process | negative regulation of MAPK cascade |
| C | 0044164 | cellular_component | host cell cytosol |
| C | 0044174 | cellular_component | host cell endosome |
| C | 0044175 | cellular_component | host cell endosome membrane |
| C | 0044533 | biological_process | positive regulation of apoptotic process in another organism |
| C | 0046872 | molecular_function | metal ion binding |
| C | 0051260 | biological_process | protein homooligomerization |
| C | 0090729 | molecular_function | toxin activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005576 | cellular_component | extracellular region |
| D | 0016020 | cellular_component | membrane |
| D | 0020002 | cellular_component | host cell plasma membrane |
| D | 0042802 | molecular_function | identical protein binding |
| D | 0043409 | biological_process | negative regulation of MAPK cascade |
| D | 0044164 | cellular_component | host cell cytosol |
| D | 0044174 | cellular_component | host cell endosome |
| D | 0044175 | cellular_component | host cell endosome membrane |
| D | 0044533 | biological_process | positive regulation of apoptotic process in another organism |
| D | 0046872 | molecular_function | metal ion binding |
| D | 0051260 | biological_process | protein homooligomerization |
| D | 0090729 | molecular_function | toxin activity |
| E | 0005515 | molecular_function | protein binding |
| E | 0005576 | cellular_component | extracellular region |
| E | 0016020 | cellular_component | membrane |
| E | 0020002 | cellular_component | host cell plasma membrane |
| E | 0042802 | molecular_function | identical protein binding |
| E | 0043409 | biological_process | negative regulation of MAPK cascade |
| E | 0044164 | cellular_component | host cell cytosol |
| E | 0044174 | cellular_component | host cell endosome |
| E | 0044175 | cellular_component | host cell endosome membrane |
| E | 0044533 | biological_process | positive regulation of apoptotic process in another organism |
| E | 0046872 | molecular_function | metal ion binding |
| E | 0051260 | biological_process | protein homooligomerization |
| E | 0090729 | molecular_function | toxin activity |
| F | 0005515 | molecular_function | protein binding |
| F | 0005576 | cellular_component | extracellular region |
| F | 0016020 | cellular_component | membrane |
| F | 0020002 | cellular_component | host cell plasma membrane |
| F | 0042802 | molecular_function | identical protein binding |
| F | 0043409 | biological_process | negative regulation of MAPK cascade |
| F | 0044164 | cellular_component | host cell cytosol |
| F | 0044174 | cellular_component | host cell endosome |
| F | 0044175 | cellular_component | host cell endosome membrane |
| F | 0044533 | biological_process | positive regulation of apoptotic process in another organism |
| F | 0046872 | molecular_function | metal ion binding |
| F | 0051260 | biological_process | protein homooligomerization |
| F | 0090729 | molecular_function | toxin activity |
| G | 0005515 | molecular_function | protein binding |
| G | 0005576 | cellular_component | extracellular region |
| G | 0016020 | cellular_component | membrane |
| G | 0020002 | cellular_component | host cell plasma membrane |
| G | 0042802 | molecular_function | identical protein binding |
| G | 0043409 | biological_process | negative regulation of MAPK cascade |
| G | 0044164 | cellular_component | host cell cytosol |
| G | 0044174 | cellular_component | host cell endosome |
| G | 0044175 | cellular_component | host cell endosome membrane |
| G | 0044533 | biological_process | positive regulation of apoptotic process in another organism |
| G | 0046872 | molecular_function | metal ion binding |
| G | 0051260 | biological_process | protein homooligomerization |
| G | 0090729 | molecular_function | toxin activity |
| H | 0003824 | molecular_function | catalytic activity |
| H | 0004016 | molecular_function | adenylate cyclase activity |
| H | 0005515 | molecular_function | protein binding |
| H | 0005516 | molecular_function | calmodulin binding |
| H | 0005524 | molecular_function | ATP binding |
| H | 0005576 | cellular_component | extracellular region |
| H | 0006171 | biological_process | cAMP biosynthetic process |
| H | 0008237 | molecular_function | metallopeptidase activity |
| H | 0008294 | molecular_function | calcium- and calmodulin-responsive adenylate cyclase activity |
| H | 0016829 | molecular_function | lyase activity |
| H | 0030430 | cellular_component | host cell cytoplasm |
| H | 0035821 | biological_process | modulation of process of another organism |
| H | 0036094 | molecular_function | small molecule binding |
| H | 0044164 | cellular_component | host cell cytosol |
| H | 0046872 | molecular_function | metal ion binding |
| H | 0090729 | molecular_function | toxin activity |
| H | 0099004 | biological_process | calmodulin dependent kinase signaling pathway |
| H | 1902494 | cellular_component | catalytic complex |
| I | 0003824 | molecular_function | catalytic activity |
| I | 0004016 | molecular_function | adenylate cyclase activity |
| I | 0005515 | molecular_function | protein binding |
| I | 0005516 | molecular_function | calmodulin binding |
| I | 0005524 | molecular_function | ATP binding |
| I | 0005576 | cellular_component | extracellular region |
| I | 0006171 | biological_process | cAMP biosynthetic process |
| I | 0008237 | molecular_function | metallopeptidase activity |
| I | 0008294 | molecular_function | calcium- and calmodulin-responsive adenylate cyclase activity |
| I | 0016829 | molecular_function | lyase activity |
| I | 0030430 | cellular_component | host cell cytoplasm |
| I | 0035821 | biological_process | modulation of process of another organism |
| I | 0036094 | molecular_function | small molecule binding |
| I | 0044164 | cellular_component | host cell cytosol |
| I | 0046872 | molecular_function | metal ion binding |
| I | 0090729 | molecular_function | toxin activity |
| I | 0099004 | biological_process | calmodulin dependent kinase signaling pathway |
| I | 1902494 | cellular_component | catalytic complex |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | binding site for residue CA A 801 |
| Chain | Residue |
| A | ASP177 |
| A | ASP179 |
| A | ASP181 |
| A | ILE183 |
| A | GLU188 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | binding site for residue CA A 802 |
| Chain | Residue |
| A | LYS225 |
| A | ASP235 |
| A | ASP179 |
| A | ASP181 |
| A | GLU188 |
| A | SER222 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | binding site for residue CA B 801 |
| Chain | Residue |
| B | ASP177 |
| B | ASP179 |
| B | ASP181 |
| B | ILE183 |
| B | ASP185 |
| B | GLU188 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | binding site for residue CA B 802 |
| Chain | Residue |
| B | ASP179 |
| B | ASP181 |
| B | GLU188 |
| B | SER222 |
| B | LYS225 |
| B | ASP235 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | binding site for residue CA C 801 |
| Chain | Residue |
| C | ASP177 |
| C | ASP179 |
| C | ASP181 |
| C | ILE183 |
| C | GLU188 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | binding site for residue CA C 802 |
| Chain | Residue |
| C | ASP179 |
| C | ASP181 |
| C | GLU188 |
| C | SER222 |
| C | LYS225 |
| C | ASP235 |
| site_id | AC7 |
| Number of Residues | 5 |
| Details | binding site for residue CA D 801 |
| Chain | Residue |
| D | ASP177 |
| D | ASP179 |
| D | ASP181 |
| D | ILE183 |
| D | GLU188 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | binding site for residue CA D 802 |
| Chain | Residue |
| D | ASP179 |
| D | ASP181 |
| D | GLU188 |
| D | SER222 |
| D | LYS225 |
| D | ASP235 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | binding site for residue CA E 801 |
| Chain | Residue |
| E | ASP177 |
| E | ASP179 |
| E | ASP181 |
| E | ILE183 |
| E | ASP185 |
| E | GLU188 |
| site_id | AD1 |
| Number of Residues | 6 |
| Details | binding site for residue CA E 802 |
| Chain | Residue |
| E | ASP179 |
| E | ASP181 |
| E | GLU188 |
| E | SER222 |
| E | LYS225 |
| E | ASP235 |
| site_id | AD2 |
| Number of Residues | 6 |
| Details | binding site for residue CA F 801 |
| Chain | Residue |
| F | ASP177 |
| F | ASP179 |
| F | ASP181 |
| F | ILE183 |
| F | ASP185 |
| F | GLU188 |
| site_id | AD3 |
| Number of Residues | 6 |
| Details | binding site for residue CA F 802 |
| Chain | Residue |
| F | ASP179 |
| F | ASP181 |
| F | GLU188 |
| F | SER222 |
| F | LYS225 |
| F | ASP235 |
| site_id | AD4 |
| Number of Residues | 6 |
| Details | binding site for residue CA G 801 |
| Chain | Residue |
| G | ASP177 |
| G | ASP179 |
| G | ASP181 |
| G | ILE183 |
| G | ASP185 |
| G | GLU188 |
| site_id | AD5 |
| Number of Residues | 6 |
| Details | binding site for residue CA G 802 |
| Chain | Residue |
| G | ASP179 |
| G | ASP181 |
| G | GLU188 |
| G | SER222 |
| G | LYS225 |
| G | ASP235 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton acceptor => ECO:0000269|PubMed:11807546 |
| Chain | Residue | Details |
| H | HIS318 | |
| E | ILE316-SER325 | |
| F | GLU302-PHE313 | |
| F | ILE316-SER325 | |
| G | GLU302-PHE313 | |
| G | ILE316-SER325 | |
| I | HIS318 | |
| B | GLU302-PHE313 | |
| B | ILE316-SER325 | |
| C | GLU302-PHE313 | |
| C | ILE316-SER325 | |
| D | GLU302-PHE313 | |
| D | ILE316-SER325 | |
| E | GLU302-PHE313 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15719022, ECO:0007744|PDB:1XFU, ECO:0007744|PDB:1XFV, ECO:0007744|PDB:1XFW, ECO:0007744|PDB:1XFX, ECO:0007744|PDB:1XFY, ECO:0007744|PDB:1XFZ, ECO:0007744|PDB:1Y0V |
| Chain | Residue | Details |
| H | ASP458 | |
| D | ASP177 | |
| D | ASP181 | |
| D | ILE183 | |
| E | ASP177 | |
| E | ASP181 | |
| E | ILE183 | |
| F | ASP177 | |
| F | ASP181 | |
| F | ILE183 | |
| G | ASP177 | |
| H | ASP460 | |
| G | ASP181 | |
| G | ILE183 | |
| H | HIS544 | |
| I | ASP458 | |
| I | ASP460 | |
| I | HIS544 | |
| C | ASP177 | |
| C | ASP181 | |
| C | ILE183 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15719022, ECO:0007744|PDB:1XFW |
| Chain | Residue | Details |
| H | THR515 | |
| I | THR515 | |
| C | ASP179 | |
| D | ASP179 | |
| E | ASP179 | |
| F | ASP179 | |
| G | ASP179 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 7 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15326297, ECO:0000269|PubMed:21037566, ECO:0000269|PubMed:25778700, ECO:0000269|PubMed:32047164, ECO:0007744|PDB:1ACC, ECO:0007744|PDB:1TZN, ECO:0007744|PDB:1TZO, ECO:0007744|PDB:3J9C, ECO:0007744|PDB:3KWV, ECO:0007744|PDB:6PSN, ECO:0007744|PDB:6UZB, ECO:0007744|PDB:6UZD, ECO:0007744|PDB:6UZE |
| Chain | Residue | Details |
| A | GLU188 | |
| B | GLU188 | |
| C | GLU188 | |
| D | GLU188 | |
| E | GLU188 | |
| F | GLU188 | |
| G | GLU188 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 14 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15243628, ECO:0000269|PubMed:15326297, ECO:0000269|PubMed:21037566, ECO:0000269|PubMed:25778700, ECO:0000269|PubMed:32047164, ECO:0007744|PDB:1ACC, ECO:0007744|PDB:1T6B, ECO:0007744|PDB:1TZN, ECO:0007744|PDB:1TZO, ECO:0007744|PDB:3J9C, ECO:0007744|PDB:3KWV, ECO:0007744|PDB:6PSN, ECO:0007744|PDB:6UZB, ECO:0007744|PDB:6UZD, ECO:0007744|PDB:6UZE |
| Chain | Residue | Details |
| A | SER222 | |
| E | LYS225 | |
| F | SER222 | |
| F | LYS225 | |
| G | SER222 | |
| G | LYS225 | |
| A | LYS225 | |
| B | SER222 | |
| B | LYS225 | |
| C | SER222 | |
| C | LYS225 | |
| D | SER222 | |
| D | LYS225 | |
| E | SER222 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 7 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15243628, ECO:0000269|PubMed:15326297, ECO:0000269|PubMed:21037566, ECO:0000269|PubMed:32047164, ECO:0007744|PDB:1ACC, ECO:0007744|PDB:1T6B, ECO:0007744|PDB:1TZN, ECO:0007744|PDB:1TZO, ECO:0007744|PDB:3KWV, ECO:0007744|PDB:6PSN, ECO:0007744|PDB:6UZB, ECO:0007744|PDB:6UZD, ECO:0007744|PDB:6UZE |
| Chain | Residue | Details |
| A | ASP235 | |
| B | ASP235 | |
| C | ASP235 | |
| D | ASP235 | |
| E | ASP235 | |
| F | ASP235 | |
| G | ASP235 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 7 |
| Details | SITE: Cleavage; by FURIN => ECO:0000269|PubMed:11207581, ECO:0000269|PubMed:1438214, ECO:0000269|PubMed:1644824 |
| Chain | Residue | Details |
| A | ARG167 | |
| B | ARG167 | |
| C | ARG167 | |
| D | ARG167 | |
| E | ARG167 | |
| F | ARG167 | |
| G | ARG167 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 28 |
| Details | SITE: Alpha-clamp => ECO:0000269|PubMed:21037566 |
| Chain | Residue | Details |
| A | ARG178 | |
| C | LEU187 | |
| C | PHE236 | |
| C | PHE464 | |
| D | ARG178 | |
| D | LEU187 | |
| D | PHE236 | |
| D | PHE464 | |
| E | ARG178 | |
| E | LEU187 | |
| E | PHE236 | |
| A | LEU187 | |
| E | PHE464 | |
| F | ARG178 | |
| F | LEU187 | |
| F | PHE236 | |
| F | PHE464 | |
| G | ARG178 | |
| G | LEU187 | |
| G | PHE236 | |
| G | PHE464 | |
| A | PHE236 | |
| A | PHE464 | |
| B | ARG178 | |
| B | LEU187 | |
| B | PHE236 | |
| B | PHE464 | |
| C | ARG178 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 7 |
| Details | SITE: Cleavage; by chymotrypsin; required for translocation of LF and EF => ECO:0000269|PubMed:7961869 |
| Chain | Residue | Details |
| A | PHE314 | |
| B | PHE314 | |
| C | PHE314 | |
| D | PHE314 | |
| E | PHE314 | |
| F | PHE314 | |
| G | PHE314 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 7 |
| Details | SITE: Phi-clamp => ECO:0000269|PubMed:16051798, ECO:0000269|PubMed:25778700 |
| Chain | Residue | Details |
| A | PHE427 | |
| B | PHE427 | |
| C | PHE427 | |
| D | PHE427 | |
| E | PHE427 | |
| F | PHE427 | |
| G | PHE427 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 7 |
| Details | SITE: Essential for binding to cell receptor => ECO:0000269|PubMed:12771151 |
| Chain | Residue | Details |
| A | ASP683 | |
| B | ASP683 | |
| C | ASP683 | |
| D | ASP683 | |
| E | ASP683 | |
| F | ASP683 | |
| G | ASP683 |
