Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6UU5

E. coli sigma-S transcription initiation complex with a 6-nt RNA ("Old" crystal soaked with GTP, UTP, CTP, and dinucleotide GpA for 30 minutes)

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
AAA	0003677	molecular_function	DNA binding
AAA	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
AAA	0006351	biological_process	DNA-templated transcription
AAA	0046983	molecular_function	protein dimerization activity
BBB	0003677	molecular_function	DNA binding
BBB	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
BBB	0006351	biological_process	DNA-templated transcription
BBB	0046983	molecular_function	protein dimerization activity
CCC	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
CCC	0000428	cellular_component	DNA-directed RNA polymerase complex
CCC	0003677	molecular_function	DNA binding
CCC	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
CCC	0005515	molecular_function	protein binding
CCC	0005737	cellular_component	cytoplasm
CCC	0005829	cellular_component	cytosol
CCC	0006351	biological_process	DNA-templated transcription
CCC	0006352	biological_process	DNA-templated transcription initiation
CCC	0006879	biological_process	intracellular iron ion homeostasis
CCC	0008023	cellular_component	transcription elongation factor complex
CCC	0009408	biological_process	response to heat
CCC	0016020	cellular_component	membrane
CCC	0016779	molecular_function	nucleotidyltransferase activity
CCC	0031564	biological_process	transcription antitermination
CCC	0032549	molecular_function	ribonucleoside binding
CCC	0032784	biological_process	regulation of DNA-templated transcription elongation
CCC	0034062	molecular_function	5'-3' RNA polymerase activity
CCC	0036460	biological_process	cellular response to cell envelope stress
CCC	0042128	biological_process	nitrate assimilation
CCC	0044780	biological_process	bacterial-type flagellum assembly
CCC	0046677	biological_process	response to antibiotic
CCC	0048870	biological_process	cell motility
CCC	0071973	biological_process	bacterial-type flagellum-dependent cell motility
CCC	0090605	biological_process	submerged biofilm formation
CCC	2000142	biological_process	regulation of DNA-templated transcription initiation
DDD	0000287	molecular_function	magnesium ion binding
DDD	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
DDD	0000428	cellular_component	DNA-directed RNA polymerase complex
DDD	0003677	molecular_function	DNA binding
DDD	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
DDD	0005515	molecular_function	protein binding
DDD	0005737	cellular_component	cytoplasm
DDD	0005829	cellular_component	cytosol
DDD	0006351	biological_process	DNA-templated transcription
DDD	0006352	biological_process	DNA-templated transcription initiation
DDD	0006879	biological_process	intracellular iron ion homeostasis
DDD	0008023	cellular_component	transcription elongation factor complex
DDD	0008270	molecular_function	zinc ion binding
DDD	0009408	biological_process	response to heat
DDD	0016020	cellular_component	membrane
DDD	0016779	molecular_function	nucleotidyltransferase activity
DDD	0031564	biological_process	transcription antitermination
DDD	0032784	biological_process	regulation of DNA-templated transcription elongation
DDD	0034062	molecular_function	5'-3' RNA polymerase activity
DDD	0036460	biological_process	cellular response to cell envelope stress
DDD	0042128	biological_process	nitrate assimilation
DDD	0044780	biological_process	bacterial-type flagellum assembly
DDD	0046677	biological_process	response to antibiotic
DDD	0046872	molecular_function	metal ion binding
DDD	0048870	biological_process	cell motility
DDD	0071973	biological_process	bacterial-type flagellum-dependent cell motility
DDD	0090605	biological_process	submerged biofilm formation
DDD	2000142	biological_process	regulation of DNA-templated transcription initiation
EEE	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
EEE	0000428	cellular_component	DNA-directed RNA polymerase complex
EEE	0001000	molecular_function	bacterial-type RNA polymerase core enzyme binding
EEE	0003677	molecular_function	DNA binding
EEE	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
EEE	0005829	cellular_component	cytosol
EEE	0006351	biological_process	DNA-templated transcription
EEE	0006352	biological_process	DNA-templated transcription initiation
EEE	0006879	biological_process	intracellular iron ion homeostasis
EEE	0008023	cellular_component	transcription elongation factor complex
EEE	0009408	biological_process	response to heat
EEE	0016779	molecular_function	nucleotidyltransferase activity
EEE	0030880	cellular_component	RNA polymerase complex
EEE	0031564	biological_process	transcription antitermination
EEE	0032784	biological_process	regulation of DNA-templated transcription elongation
EEE	0034062	molecular_function	5'-3' RNA polymerase activity
EEE	0036460	biological_process	cellular response to cell envelope stress
EEE	0042128	biological_process	nitrate assimilation
EEE	0044780	biological_process	bacterial-type flagellum assembly
EEE	0048870	biological_process	cell motility
EEE	0065003	biological_process	protein-containing complex assembly
EEE	0071973	biological_process	bacterial-type flagellum-dependent cell motility
EEE	0090605	biological_process	submerged biofilm formation
EEE	2000142	biological_process	regulation of DNA-templated transcription initiation
FFF	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
FFF	0001000	molecular_function	bacterial-type RNA polymerase core enzyme binding
FFF	0003677	molecular_function	DNA binding
FFF	0003700	molecular_function	DNA-binding transcription factor activity
FFF	0005515	molecular_function	protein binding
FFF	0005737	cellular_component	cytoplasm
FFF	0006351	biological_process	DNA-templated transcription
FFF	0006352	biological_process	DNA-templated transcription initiation
FFF	0006355	biological_process	regulation of DNA-templated transcription
FFF	0006950	biological_process	response to stress
FFF	0010468	biological_process	regulation of gene expression
FFF	0016987	molecular_function	sigma factor activity
FFF	0045892	biological_process	negative regulation of DNA-templated transcription
FFF	1903865	cellular_component	sigma factor antagonist complex
FFF	2000142	biological_process	regulation of DNA-templated transcription initiation

Functional Information from PROSITE/UniProt

site_id	PS00715
Number of Residues	14
Details	SIGMA70_1 Sigma-70 factors family signature 1. DLIEeGnLGLIrAV

Chain	Residue	Details
FFF	ASP118-VAL131

site_id	PS00716
Number of Residues	27
Details	SIGMA70_2 Sigma-70 factors family signature 2. TleDVGreigLTrerVrQIQvegLrrL

Chain	Residue	Details
FFF	THR287-LEU313

site_id	PS01166
Number of Residues	13
Details	RNA_POL_BETA RNA polymerases beta chain signature. GdKMAGrHGNKGV

Chain	Residue	Details
CCC	GLY1063-VAL1075

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	19
Details	DNA_BIND: H-T-H motif => ECO:0000255\|HAMAP-Rule:MF_00959

Chain	Residue	Details
FFF	LEU288-VAL307
DDD	CYS895
DDD	CYS898
DDD	CYS72
DDD	CYS85
DDD	CYS88
DDD	ASP460
DDD	ASP462
DDD	ASP464
DDD	CYS814
DDD	CYS888

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0000255\|HAMAP-Rule:MF_01322, ECO:0000269\|PubMed:18723842

Chain	Residue	Details
DDD	LYS983

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)