Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6U9D

Saccharomyces cerevisiae acetohydroxyacid synthase

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0003824	molecular_function	catalytic activity
A	0003984	molecular_function	acetolactate synthase activity
A	0005515	molecular_function	protein binding
A	0005739	cellular_component	mitochondrion
A	0005948	cellular_component	acetolactate synthase complex
A	0008652	biological_process	amino acid biosynthetic process
A	0009082	biological_process	branched-chain amino acid biosynthetic process
A	0009097	biological_process	isoleucine biosynthetic process
A	0009099	biological_process	L-valine biosynthetic process
A	0016740	molecular_function	transferase activity
A	0030976	molecular_function	thiamine pyrophosphate binding
A	0046872	molecular_function	metal ion binding
A	0050660	molecular_function	flavin adenine dinucleotide binding
B	0000287	molecular_function	magnesium ion binding
B	0003824	molecular_function	catalytic activity
B	0003984	molecular_function	acetolactate synthase activity
B	0005515	molecular_function	protein binding
B	0005739	cellular_component	mitochondrion
B	0005948	cellular_component	acetolactate synthase complex
B	0008652	biological_process	amino acid biosynthetic process
B	0009082	biological_process	branched-chain amino acid biosynthetic process
B	0009097	biological_process	isoleucine biosynthetic process
B	0009099	biological_process	L-valine biosynthetic process
B	0016740	molecular_function	transferase activity
B	0030976	molecular_function	thiamine pyrophosphate binding
B	0046872	molecular_function	metal ion binding
B	0050660	molecular_function	flavin adenine dinucleotide binding
C	0009082	biological_process	branched-chain amino acid biosynthetic process
C	1990610	molecular_function	acetolactate synthase regulator activity
D	0009082	biological_process	branched-chain amino acid biosynthetic process
D	1990610	molecular_function	acetolactate synthase regulator activity
E	0000287	molecular_function	magnesium ion binding
E	0003824	molecular_function	catalytic activity
E	0003984	molecular_function	acetolactate synthase activity
E	0005515	molecular_function	protein binding
E	0005739	cellular_component	mitochondrion
E	0005948	cellular_component	acetolactate synthase complex
E	0008652	biological_process	amino acid biosynthetic process
E	0009082	biological_process	branched-chain amino acid biosynthetic process
E	0009097	biological_process	isoleucine biosynthetic process
E	0009099	biological_process	L-valine biosynthetic process
E	0016740	molecular_function	transferase activity
E	0030976	molecular_function	thiamine pyrophosphate binding
E	0046872	molecular_function	metal ion binding
E	0050660	molecular_function	flavin adenine dinucleotide binding
F	0000287	molecular_function	magnesium ion binding
F	0003824	molecular_function	catalytic activity
F	0003984	molecular_function	acetolactate synthase activity
F	0005515	molecular_function	protein binding
F	0005739	cellular_component	mitochondrion
F	0005948	cellular_component	acetolactate synthase complex
F	0008652	biological_process	amino acid biosynthetic process
F	0009082	biological_process	branched-chain amino acid biosynthetic process
F	0009097	biological_process	isoleucine biosynthetic process
F	0009099	biological_process	L-valine biosynthetic process
F	0016740	molecular_function	transferase activity
F	0030976	molecular_function	thiamine pyrophosphate binding
F	0046872	molecular_function	metal ion binding
F	0050660	molecular_function	flavin adenine dinucleotide binding
G	0009082	biological_process	branched-chain amino acid biosynthetic process
G	1990610	molecular_function	acetolactate synthase regulator activity
H	0009082	biological_process	branched-chain amino acid biosynthetic process
H	1990610	molecular_function	acetolactate synthase regulator activity
I	0000287	molecular_function	magnesium ion binding
I	0003824	molecular_function	catalytic activity
I	0003984	molecular_function	acetolactate synthase activity
I	0005515	molecular_function	protein binding
I	0005739	cellular_component	mitochondrion
I	0005948	cellular_component	acetolactate synthase complex
I	0008652	biological_process	amino acid biosynthetic process
I	0009082	biological_process	branched-chain amino acid biosynthetic process
I	0009097	biological_process	isoleucine biosynthetic process
I	0009099	biological_process	L-valine biosynthetic process
I	0016740	molecular_function	transferase activity
I	0030976	molecular_function	thiamine pyrophosphate binding
I	0046872	molecular_function	metal ion binding
I	0050660	molecular_function	flavin adenine dinucleotide binding
J	0000287	molecular_function	magnesium ion binding
J	0003824	molecular_function	catalytic activity
J	0003984	molecular_function	acetolactate synthase activity
J	0005515	molecular_function	protein binding
J	0005739	cellular_component	mitochondrion
J	0005948	cellular_component	acetolactate synthase complex
J	0008652	biological_process	amino acid biosynthetic process
J	0009082	biological_process	branched-chain amino acid biosynthetic process
J	0009097	biological_process	isoleucine biosynthetic process
J	0009099	biological_process	L-valine biosynthetic process
J	0016740	molecular_function	transferase activity
J	0030976	molecular_function	thiamine pyrophosphate binding
J	0046872	molecular_function	metal ion binding
J	0050660	molecular_function	flavin adenine dinucleotide binding
K	0009082	biological_process	branched-chain amino acid biosynthetic process
K	1990610	molecular_function	acetolactate synthase regulator activity
L	0009082	biological_process	branched-chain amino acid biosynthetic process
L	1990610	molecular_function	acetolactate synthase regulator activity
M	0000287	molecular_function	magnesium ion binding
M	0003824	molecular_function	catalytic activity
M	0003984	molecular_function	acetolactate synthase activity
M	0005515	molecular_function	protein binding
M	0005739	cellular_component	mitochondrion
M	0005948	cellular_component	acetolactate synthase complex
M	0008652	biological_process	amino acid biosynthetic process
M	0009082	biological_process	branched-chain amino acid biosynthetic process
M	0009097	biological_process	isoleucine biosynthetic process
M	0009099	biological_process	L-valine biosynthetic process
M	0016740	molecular_function	transferase activity
M	0030976	molecular_function	thiamine pyrophosphate binding
M	0046872	molecular_function	metal ion binding
M	0050660	molecular_function	flavin adenine dinucleotide binding
N	0000287	molecular_function	magnesium ion binding
N	0003824	molecular_function	catalytic activity
N	0003984	molecular_function	acetolactate synthase activity
N	0005515	molecular_function	protein binding
N	0005739	cellular_component	mitochondrion
N	0005948	cellular_component	acetolactate synthase complex
N	0008652	biological_process	amino acid biosynthetic process
N	0009082	biological_process	branched-chain amino acid biosynthetic process
N	0009097	biological_process	isoleucine biosynthetic process
N	0009099	biological_process	L-valine biosynthetic process
N	0016740	molecular_function	transferase activity
N	0030976	molecular_function	thiamine pyrophosphate binding
N	0046872	molecular_function	metal ion binding
N	0050660	molecular_function	flavin adenine dinucleotide binding
O	0009082	biological_process	branched-chain amino acid biosynthetic process
O	1990610	molecular_function	acetolactate synthase regulator activity
P	0009082	biological_process	branched-chain amino acid biosynthetic process
P	1990610	molecular_function	acetolactate synthase regulator activity
Q	0000287	molecular_function	magnesium ion binding
Q	0003824	molecular_function	catalytic activity
Q	0003984	molecular_function	acetolactate synthase activity
Q	0005515	molecular_function	protein binding
Q	0005739	cellular_component	mitochondrion
Q	0005948	cellular_component	acetolactate synthase complex
Q	0008652	biological_process	amino acid biosynthetic process
Q	0009082	biological_process	branched-chain amino acid biosynthetic process
Q	0009097	biological_process	isoleucine biosynthetic process
Q	0009099	biological_process	L-valine biosynthetic process
Q	0016740	molecular_function	transferase activity
Q	0030976	molecular_function	thiamine pyrophosphate binding
Q	0046872	molecular_function	metal ion binding
Q	0050660	molecular_function	flavin adenine dinucleotide binding
R	0000287	molecular_function	magnesium ion binding
R	0003824	molecular_function	catalytic activity
R	0003984	molecular_function	acetolactate synthase activity
R	0005515	molecular_function	protein binding
R	0005739	cellular_component	mitochondrion
R	0005948	cellular_component	acetolactate synthase complex
R	0008652	biological_process	amino acid biosynthetic process
R	0009082	biological_process	branched-chain amino acid biosynthetic process
R	0009097	biological_process	isoleucine biosynthetic process
R	0009099	biological_process	L-valine biosynthetic process
R	0016740	molecular_function	transferase activity
R	0030976	molecular_function	thiamine pyrophosphate binding
R	0046872	molecular_function	metal ion binding
R	0050660	molecular_function	flavin adenine dinucleotide binding
S	0009082	biological_process	branched-chain amino acid biosynthetic process
S	1990610	molecular_function	acetolactate synthase regulator activity
T	0009082	biological_process	branched-chain amino acid biosynthetic process
T	1990610	molecular_function	acetolactate synthase regulator activity
U	0000287	molecular_function	magnesium ion binding
U	0003824	molecular_function	catalytic activity
U	0003984	molecular_function	acetolactate synthase activity
U	0005515	molecular_function	protein binding
U	0005739	cellular_component	mitochondrion
U	0005948	cellular_component	acetolactate synthase complex
U	0008652	biological_process	amino acid biosynthetic process
U	0009082	biological_process	branched-chain amino acid biosynthetic process
U	0009097	biological_process	isoleucine biosynthetic process
U	0009099	biological_process	L-valine biosynthetic process
U	0016740	molecular_function	transferase activity
U	0030976	molecular_function	thiamine pyrophosphate binding
U	0046872	molecular_function	metal ion binding
U	0050660	molecular_function	flavin adenine dinucleotide binding
V	0000287	molecular_function	magnesium ion binding
V	0003824	molecular_function	catalytic activity
V	0003984	molecular_function	acetolactate synthase activity
V	0005515	molecular_function	protein binding
V	0005739	cellular_component	mitochondrion
V	0005948	cellular_component	acetolactate synthase complex
V	0008652	biological_process	amino acid biosynthetic process
V	0009082	biological_process	branched-chain amino acid biosynthetic process
V	0009097	biological_process	isoleucine biosynthetic process
V	0009099	biological_process	L-valine biosynthetic process
V	0016740	molecular_function	transferase activity
V	0030976	molecular_function	thiamine pyrophosphate binding
V	0046872	molecular_function	metal ion binding
V	0050660	molecular_function	flavin adenine dinucleotide binding
W	0009082	biological_process	branched-chain amino acid biosynthetic process
W	1990610	molecular_function	acetolactate synthase regulator activity
X	0009082	biological_process	branched-chain amino acid biosynthetic process
X	1990610	molecular_function	acetolactate synthase regulator activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	22
Details	binding site for residue TPP A 701

Chain	Residue
A	VAL497
A	ASN577
A	GLU579
A	GLN580
A	GLY581
A	MET582
A	VAL583
A	MG702
B	TYR113
B	PRO114
B	GLU139
A	GLY498
B	THR162
B	PRO165
B	GLN202
A	GLN499
A	HIS500
A	GLY523
A	MET525
A	ASP550
A	ALA551
A	SER552

site_id	AC2
Number of Residues	4
Details	binding site for residue MG A 702

Chain	Residue
A	ASP550
A	ASN577
A	GLU579
A	TPP701

site_id	AC3
Number of Residues	32
Details	binding site for residue FAD A 703

Chain	Residue
A	ASP180
A	ARG241
A	GLY307
A	ALA308
A	GLY309
A	ASN312
A	THR334
A	LEU335
A	GLN336
A	LEU352
A	MET354
A	HIS355
A	GLY374
A	ALA375
A	ARG376
A	ASP378
A	ARG380
A	VAL381
A	GLU407
A	VAL408
A	SER409
A	ASN412
A	GLY425
A	ASP426
A	ALA427
A	GLN501
A	MET502
A	SER519
A	GLY520
A	GLY521
A	60G704
B	PHE201

site_id	AC4
Number of Residues	15
Details	binding site for residue 60G A 704

Chain	Residue
A	MET354
A	ASP379
A	ARG380
A	MET582
A	VAL583
A	TRP586
A	FAD703
A	HOH803
B	GLY116
B	ALA117
B	VAL191
B	PRO192
B	PHE201
B	GLN202
B	LYS251

site_id	AC5
Number of Residues	22
Details	binding site for residue TPP B 701

Chain	Residue
A	TYR113
A	PRO114
A	GLU139
A	THR162
A	PRO165
A	GLN202
B	VAL497
B	GLY498
B	GLN499
B	HIS500
B	GLY523
B	MET525
B	ASP550
B	ALA551
B	SER552
B	ASN577
B	GLU579
B	GLN580
B	GLY581
B	MET582
B	VAL583
B	MG702

site_id	AC6
Number of Residues	4
Details	binding site for residue MG B 702

Chain	Residue
B	ASP550
B	ASN577
B	GLU579
B	TPP701

site_id	AC7
Number of Residues	31
Details	binding site for residue FAD B 703

Chain	Residue
A	PHE201
B	ASP180
B	ARG241
B	GLY307
B	ALA308
B	GLY309
B	ASN312
B	THR334
B	LEU335
B	GLN336
B	LEU352
B	GLY353
B	MET354
B	HIS355
B	GLY374
B	ALA375
B	ARG376
B	ASP378
B	ARG380
B	VAL381
B	GLU407
B	VAL408
B	ASN412
B	GLY425
B	ASP426
B	ALA427
B	GLN501
B	MET502
B	SER519
B	GLY520
B	GLY521

site_id	AC8
Number of Residues	12
Details	binding site for residue 60G B 704

Chain	Residue
A	GLY116
A	ALA117
A	VAL191
A	PRO192
A	ALA200
A	PHE201
A	LYS251
B	MET354
B	ASP379
B	ARG380
B	MET582
B	TRP586

site_id	AC9
Number of Residues	14
Details	binding site for residue ATP C 401

Chain	Residue
C	ASN231
C	PHE232
C	LYS251
C	ARG254
C	ALA257
C	MG402
O	VAL237
O	ASP238
O	ILE239
P	ARG159
P	LYS251
P	ARG254
P	ARG280
P	ATP401

site_id	AD1
Number of Residues	4
Details	binding site for residue MG C 402

Chain	Residue
C	ARG254
C	ATP401
P	ARG254
P	ATP401

site_id	AD2
Number of Residues	13
Details	binding site for residue ATP D 401

Chain	Residue
D	ASN231
D	PHE232
D	LYS251
D	ARG254
D	ALA257
D	MG402
G	ARG159
G	LYS251
G	ARG254
G	ATP401
H	VAL237
H	ASP238
H	ILE239

site_id	AD3
Number of Residues	4
Details	binding site for residue MG D 402

Chain	Residue
D	ARG254
D	ATP401
G	ARG254
G	ATP401

site_id	AD4
Number of Residues	22
Details	binding site for residue TPP E 701

Chain	Residue
E	VAL497
E	GLN499
E	HIS500
E	GLY523
E	MET525
E	GLY549
E	ASP550
E	ALA551
E	SER552
E	ASN577
E	GLU579
E	GLN580
E	GLY581
E	VAL583
E	MG702
F	TYR113
F	PRO114
F	GLU139
F	THR162
F	PRO165
F	ASN169
F	GLN202

site_id	AD5
Number of Residues	4
Details	binding site for residue MG E 702

Chain	Residue
E	ASP550
E	ASN577
E	GLU579
E	TPP701

site_id	AD6
Number of Residues	32
Details	binding site for residue FAD E 703

Chain	Residue
E	ASP180
E	ARG241
E	GLY307
E	ALA308
E	GLY309
E	ASN312
E	THR334
E	LEU335
E	GLN336
E	LEU352
E	MET354
E	HIS355
E	GLY374
E	ALA375
E	ARG376
E	ASP378
E	ARG380
E	VAL381
E	GLU407
E	VAL408
E	SER409
E	ASN412
E	GLY425
E	ASP426
E	ALA427
E	GLN501
E	MET502
E	SER519
E	GLY520
E	GLY521
E	60G704
F	PHE201

site_id	AD7
Number of Residues	13
Details	binding site for residue 60G E 704

Chain	Residue
E	MET354
E	ASP379
E	ARG380
E	MET582
E	TRP586
E	FAD703
F	GLY116
F	ALA117
F	VAL191
F	PRO192
F	ALA200
F	PHE201
F	LYS251

site_id	AD8
Number of Residues	22
Details	binding site for residue TPP F 701

Chain	Residue
E	TYR113
E	PRO114
E	GLU139
E	PRO165
E	ASN169
E	GLN202
F	VAL497
F	GLY498
F	GLN499
F	HIS500
F	GLY523
F	MET525
F	ASP550
F	ALA551
F	SER552
F	ASN577
F	GLU579
F	GLN580
F	GLY581
F	MET582
F	VAL583
F	MG702

site_id	AD9
Number of Residues	5
Details	binding site for residue MG F 702

Chain	Residue
F	ASP550
F	LEU575
F	ASN577
F	GLU579
F	TPP701

site_id	AE1
Number of Residues	32
Details	binding site for residue FAD F 703

Chain	Residue
E	PHE201
F	ASP180
F	ARG241
F	GLY307
F	ALA308
F	GLY309
F	ASN312
F	THR334
F	LEU335
F	GLN336
F	LEU352
F	GLY353
F	MET354
F	HIS355
F	GLY374
F	ALA375
F	ARG376
F	ASP378
F	ARG380
F	VAL381
F	PHE406
F	GLU407
F	VAL408
F	ASN412
F	GLY425
F	ASP426
F	ALA427
F	GLN501
F	MET502
F	SER519
F	GLY520
F	GLY521

site_id	AE2
Number of Residues	12
Details	binding site for residue 60G F 704

Chain	Residue
E	GLY116
E	ALA117
E	VAL191
E	PRO192
E	PHE201
E	LYS251
F	MET354
F	ASP379
F	ARG380
F	MET582
F	VAL583
F	TRP586

site_id	AE3
Number of Residues	14
Details	binding site for residue ATP G 401

Chain	Residue
C	VAL237
C	ASP238
C	ILE239
D	ARG159
D	LYS251
D	ARG254
D	ARG280
D	ATP401
D	MG402
G	ASN231
G	PHE232
G	LYS251
G	ARG254
G	ALA257

site_id	AE4
Number of Residues	15
Details	binding site for residue ATP H 401

Chain	Residue
H	ASN231
H	PHE232
H	LYS251
H	ARG254
H	ALA257
H	MG402
K	GLU155
K	ARG159
K	LYS251
K	ARG254
K	ARG280
K	ATP401
L	VAL237
L	ASP238
L	ILE239

site_id	AE5
Number of Residues	2
Details	binding site for residue MG H 402

Chain	Residue
H	ATP401
K	ATP401

site_id	AE6
Number of Residues	20
Details	binding site for residue TPP I 701

Chain	Residue
I	VAL497
I	GLY498
I	GLN499
I	HIS500
I	GLY523
I	MET525
I	ASP550
I	ALA551
I	SER552
I	ASN577
I	GLU579
I	GLN580
I	GLY581
I	MET582
I	VAL583
I	MG702
J	PRO114
J	GLU139
J	PRO165
J	GLN202

site_id	AE7
Number of Residues	4
Details	binding site for residue MG I 702

Chain	Residue
I	ASP550
I	ASN577
I	GLU579
I	TPP701

site_id	AE8
Number of Residues	31
Details	binding site for residue FAD I 703

Chain	Residue
I	ASP180
I	ARG241
I	GLY307
I	ALA308
I	GLY309
I	ASN312
I	THR334
I	LEU335
I	GLN336
I	LEU352
I	GLY353
I	MET354
I	HIS355
I	GLY374
I	ALA375
I	ARG376
I	ASP378
I	ARG380
I	VAL381
I	GLU407
I	VAL408
I	ASN412
I	ASP426
I	ALA427
I	GLN501
I	MET502
I	SER519
I	GLY520
I	GLY521
I	60G704
J	PHE201

site_id	AE9
Number of Residues	13
Details	binding site for residue 60G I 704

Chain	Residue
I	MET354
I	ASP379
I	ARG380
I	MET582
I	VAL583
I	TRP586
I	FAD703
J	GLY116
J	ALA117
J	VAL191
J	ALA200
J	PHE201
J	LYS251

site_id	AF1
Number of Residues	22
Details	binding site for residue TPP J 701

Chain	Residue
I	TYR113
I	PRO114
I	GLY115
I	GLU139
I	PRO165
I	ASN169
I	GLN202
J	VAL497
J	GLN499
J	HIS500
J	GLY523
J	MET525
J	ASP550
J	ALA551
J	SER552
J	ASN577
J	GLU579
J	GLN580
J	GLY581
J	MET582
J	VAL583
J	MG702

site_id	AF2
Number of Residues	4
Details	binding site for residue MG J 702

Chain	Residue
J	ASP550
J	ASN577
J	GLU579
J	TPP701

site_id	AF3
Number of Residues	31
Details	binding site for residue FAD J 703

Chain	Residue
I	PHE201
J	ASP180
J	ARG241
J	GLY307
J	ALA308
J	GLY309
J	ASN312
J	THR334
J	LEU335
J	LEU352
J	GLY353
J	MET354
J	HIS355
J	GLY374
J	ALA375
J	ARG376
J	ASP378
J	ARG380
J	VAL381
J	GLU407
J	VAL408
J	SER409
J	ASN412
J	GLY425
J	ASP426
J	ALA427
J	GLN501
J	MET502
J	SER519
J	GLY520
J	GLY521

site_id	AF4
Number of Residues	13
Details	binding site for residue 60G J 704

Chain	Residue
I	GLY116
I	ALA117
I	VAL191
I	PRO192
I	ALA200
I	PHE201
I	LYS251
J	MET354
J	ASP379
J	ARG380
J	MET582
J	VAL583
J	TRP586

site_id	AF5
Number of Residues	14
Details	binding site for residue ATP K 401

Chain	Residue
G	VAL237
G	ASP238
G	ILE239
H	ARG159
H	LYS251
H	ARG254
H	ARG280
H	ATP401
H	MG402
K	ASN231
K	PHE232
K	LYS251
K	ARG254
K	ALA257

site_id	AF6
Number of Residues	14
Details	binding site for residue ATP L 401

Chain	Residue
L	ASN231
L	PHE232
L	LYS251
L	ARG254
L	ALA257
L	MG402
O	ARG159
O	LYS251
O	ARG254
O	ARG280
O	ATP401
P	VAL237
P	ASP238
P	ILE239

site_id	AF7
Number of Residues	3
Details	binding site for residue MG L 402

Chain	Residue
L	ARG254
L	ATP401
O	ATP401

site_id	AF8
Number of Residues	21
Details	binding site for residue TPP M 701

Chain	Residue
M	VAL497
M	GLY498
M	GLN499
M	HIS500
M	GLY523
M	MET525
M	ASP550
M	ALA551
M	SER552
M	ASN577
M	GLU579
M	GLN580
M	GLY581
M	MET582
M	VAL583
M	MG702
N	TYR113
N	PRO114
N	GLU139
N	PRO165
N	GLN202

site_id	AF9
Number of Residues	4
Details	binding site for residue MG M 702

Chain	Residue
M	ASP550
M	ASN577
M	GLU579
M	TPP701

site_id	AG1
Number of Residues	32
Details	binding site for residue FAD M 703

Chain	Residue
M	ASP180
M	ARG241
M	GLY307
M	ALA308
M	GLY309
M	ASN312
M	THR334
M	LEU335
M	GLN336
M	LEU352
M	GLY353
M	MET354
M	HIS355
M	GLY374
M	ALA375
M	ARG376
M	ASP378
M	ARG380
M	VAL381
M	GLU407
M	VAL408
M	SER409
M	ASN412
M	ASP426
M	ALA427
M	GLN501
M	MET502
M	SER519
M	GLY520
M	GLY521
N	PHE201
N	60G704

site_id	AG2
Number of Residues	20
Details	binding site for residue TPP N 701

Chain	Residue
M	PRO114
M	GLU139
M	PRO165
M	ASN169
M	GLN202
N	VAL497
N	HIS500
N	GLY523
N	MET525
N	GLY549
N	ASP550
N	ALA551
N	SER552
N	ASN577
N	GLU579
N	GLN580
N	GLY581
N	VAL583
N	MG702
N	HOH802

site_id	AG3
Number of Residues	4
Details	binding site for residue MG N 702

Chain	Residue
N	ASP550
N	ASN577
N	GLU579
N	TPP701

site_id	AG4
Number of Residues	30
Details	binding site for residue FAD N 703

Chain	Residue
M	PHE201
N	ASP180
N	ARG241
N	GLY307
N	ALA308
N	GLY309
N	ASN312
N	THR334
N	LEU335
N	LEU352
N	GLY353
N	MET354
N	HIS355
N	GLY374
N	ALA375
N	ARG376
N	ASP378
N	ARG380
N	VAL381
N	GLU407
N	VAL408
N	ASN412
N	ASP426
N	ALA427
N	GLN501
N	MET502
N	SER519
N	GLY520
N	GLY521
N	60G705

site_id	AG5
Number of Residues	14
Details	binding site for residue 60G N 704

Chain	Residue
M	MET354
M	ASP379
M	ARG380
M	MET582
M	VAL583
M	TRP586
M	FAD703
N	GLY116
N	ALA117
N	VAL191
N	PRO192
N	PHE201
N	GLN202
N	LYS251

site_id	AG6
Number of Residues	13
Details	binding site for residue 60G N 705

Chain	Residue
M	GLY116
M	ALA117
M	VAL191
M	PRO192
M	ALA200
M	PHE201
M	LYS251
N	MET354
N	ARG380
N	MET582
N	VAL583
N	TRP586
N	FAD703

site_id	AG7
Number of Residues	14
Details	binding site for residue ATP O 401

Chain	Residue
K	VAL237
K	ASP238
K	ILE239
L	ARG159
L	LYS251
L	ARG254
L	ARG280
L	ATP401
L	MG402
O	ASN231
O	PHE232
O	LYS251
O	ARG254
O	ALA257

site_id	AG8
Number of Residues	13
Details	binding site for residue ATP P 401

Chain	Residue
C	ARG159
C	LYS251
C	ARG254
C	ARG280
C	ATP401
C	MG402
D	ASP238
D	ILE239
P	ASN231
P	PHE232
P	LYS251
P	ARG254
P	ALA257

site_id	AG9
Number of Residues	21
Details	binding site for residue TPP Q 701

Chain	Residue
Q	VAL497
Q	GLY498
Q	GLN499
Q	HIS500
Q	GLY523
Q	MET525
Q	ASP550
Q	ALA551
Q	SER552
Q	GLU579
Q	GLN580
Q	GLY581
Q	MET582
Q	VAL583
Q	MG702
Q	60G704
R	PRO114
R	GLU139
R	PRO165
R	ASN169
R	GLN202

site_id	AH1
Number of Residues	4
Details	binding site for residue MG Q 702

Chain	Residue
Q	ASP550
Q	LEU575
Q	GLU579
Q	TPP701

site_id	AH2
Number of Residues	33
Details	binding site for residue FAD Q 703

Chain	Residue
Q	ASP180
Q	ARG241
Q	GLY307
Q	ALA308
Q	GLY309
Q	ASN312
Q	THR334
Q	LEU335
Q	GLN336
Q	LEU352
Q	GLY353
Q	MET354
Q	HIS355
Q	GLY374
Q	ALA375
Q	ARG376
Q	ASP378
Q	ARG380
Q	VAL381
Q	GLU407
Q	VAL408
Q	SER409
Q	ASN412
Q	GLY425
Q	ASP426
Q	ALA427
Q	GLN501
Q	MET502
Q	SER519
Q	GLY520
Q	GLY521
Q	60G704
R	PHE201

site_id	AH3
Number of Residues	13
Details	binding site for residue 60G Q 704

Chain	Residue
Q	MET354
Q	ASP379
Q	ARG380
Q	MET582
Q	TRP586
Q	TPP701
Q	FAD703
R	GLY116
R	ALA117
R	VAL191
R	PRO192
R	PHE201
R	LYS251

site_id	AH4
Number of Residues	18
Details	binding site for residue FAD R 701

Chain	Residue
Q	PHE201
R	ASP180
R	ARG241
R	GLY309
R	ASN312
R	THR334
R	LEU335
R	GLN336
R	GLY374
R	ARG376
R	ASP378
R	ARG380
R	VAL381
R	GLU407
R	VAL408
R	ASN412
R	ASP426
R	ALA427

site_id	AH5
Number of Residues	11
Details	binding site for residue 60G R 702

Chain	Residue
Q	GLY116
Q	ALA117
Q	VAL191
Q	PRO192
Q	PHE201
Q	GLN202
Q	LYS251
R	ASP379
R	ARG380
R	VAL583
R	TRP586

site_id	AH6
Number of Residues	12
Details	binding site for residue ATP S 401

Chain	Residue
S	ARG159
S	ASN231
S	PHE232
S	LYS251
S	LYS251
S	ARG254
S	ARG254
S	ALA257
S	ARG280
T	VAL237
T	ASP238
T	ILE239

site_id	AH7
Number of Residues	13
Details	binding site for residue ATP T 401

Chain	Residue
T	ASN231
T	PHE232
T	LYS251
T	ARG254
W	ARG159
W	LYS251
W	ARG254
W	ARG280
W	ATP402
W	MG403
X	VAL237
X	ASP238
X	ILE239

site_id	AH8
Number of Residues	23
Details	binding site for residue TPP U 701

Chain	Residue
U	VAL497
U	GLY498
U	GLN499
U	HIS500
U	GLY523
U	MET525
U	GLY549
U	ASP550
U	ALA551
U	SER552
U	ASN577
U	GLU579
U	GLN580
U	GLY581
U	VAL583
U	MG702
V	TYR113
V	PRO114
V	GLU139
V	THR162
V	PRO165
V	ASN169
V	GLN202

site_id	AH9
Number of Residues	4
Details	binding site for residue MG U 702

Chain	Residue
U	ASP550
U	ASN577
U	GLU579
U	TPP701

site_id	AI1
Number of Residues	32
Details	binding site for residue FAD U 703

Chain	Residue
U	ALA179
U	ASP180
U	ARG241
U	GLY307
U	ALA308
U	GLY309
U	ASN312
U	THR334
U	LEU335
U	GLN336
U	LEU352
U	MET354
U	HIS355
U	GLY374
U	ALA375
U	ARG376
U	ASP378
U	ARG380
U	VAL381
U	GLU407
U	VAL408
U	SER409
U	ASN412
U	ASP426
U	ALA427
U	GLN501
U	MET502
U	SER519
U	GLY520
U	GLY521
U	60G704
V	PHE201

site_id	AI2
Number of Residues	13
Details	binding site for residue 60G U 704

Chain	Residue
U	MET354
U	ASP379
U	ARG380
U	MET582
U	TRP586
U	FAD703
V	GLY116
V	ALA117
V	VAL191
V	PRO192
V	ALA200
V	PHE201
V	LYS251

site_id	AI3
Number of Residues	22
Details	binding site for residue TPP V 701

Chain	Residue
U	TYR113
U	PRO114
U	GLU139
U	THR162
U	PRO165
U	ASN169
U	GLN202
V	VAL497
V	GLN499
V	HIS500
V	GLY523
V	MET525
V	ASP550
V	ALA551
V	SER552
V	ASN577
V	GLU579
V	GLN580
V	GLY581
V	MET582
V	VAL583
V	MG702

site_id	AI4
Number of Residues	6
Details	binding site for residue MG V 702

Chain	Residue
V	GLY549
V	ASP550
V	LEU575
V	ASN577
V	GLU579
V	TPP701

site_id	AI5
Number of Residues	30
Details	binding site for residue FAD V 703

Chain	Residue
U	PHE201
V	ASP180
V	ARG241
V	GLY307
V	ALA308
V	GLY309
V	ASN312
V	THR334
V	LEU335
V	GLN336
V	LEU352
V	GLY353
V	MET354
V	HIS355
V	GLY374
V	ARG376
V	ASP378
V	ARG380
V	VAL381
V	GLU407
V	VAL408
V	ASN412
V	GLY425
V	ASP426
V	ALA427
V	GLN501
V	MET502
V	GLY520
V	GLY521
V	60G704

site_id	AI6
Number of Residues	12
Details	binding site for residue 60G V 704

Chain	Residue
U	GLY116
U	ALA117
U	VAL191
U	PRO192
U	PHE201
U	LYS251
V	MET354
V	ASP379
V	ARG380
V	MET582
V	TRP586
V	FAD703

site_id	AI7
Number of Residues	12
Details	binding site for residue ATP W 401

Chain	Residue
W	VAL237
W	ASP238
W	ILE239
X	ARG159
X	ASN231
X	PHE232
X	LYS251
X	LYS251
X	ARG254
X	ARG254
X	ALA257
X	ARG280

site_id	AI8
Number of Residues	14
Details	binding site for residue ATP W 402

Chain	Residue
S	VAL237
S	ASP238
S	ILE239
T	ARG159
T	LYS251
T	ARG254
T	ARG280
T	ATP401
W	ASN231
W	PHE232
W	LYS251
W	ARG254
W	ALA257
W	MG403

site_id	AI9
Number of Residues	3
Details	binding site for residue MG W 403

Chain	Residue
T	ATP401
W	ARG254
W	ATP402

Functional Information from PROSITE/UniProt

site_id	PS00187
Number of Residues	20
Details	TPP_ENZYMES Thiamine pyrophosphate enzymes signature. IGaqvakPeslvIdIdGDAS

Chain	Residue	Details
A	ILE533-SER552

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	880
Details	Region: {"description":"Thiamine pyrophosphate binding"}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	46
Details	Binding site: {}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	471
Details	Binding site: {"evidences":[{"source":"PubMed","id":"12496246","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	960
Details	Domain: {"description":"ACT","evidences":[{"source":"PROSITE-ProRule","id":"PRU01007","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 289

Chain	Residue	Details
A	GLU139	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	PHE201	single electron acceptor, single electron donor, single electron relay
A	GLN202	electrostatic stabiliser, hydrogen bond donor
A	LYS251	steric locator
A	MET582	polar interaction, steric role

site_id	MCSA10
Number of Residues	4
Details	M-CSA 289

Chain	Residue	Details
R	GLU139	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
R	PHE201	single electron acceptor, single electron donor, single electron relay
R	GLN202	electrostatic stabiliser, hydrogen bond donor
R	LYS251	steric locator

site_id	MCSA11
Number of Residues	5
Details	M-CSA 289

Chain	Residue	Details
U	GLU139	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
U	PHE201	single electron acceptor, single electron donor, single electron relay
U	GLN202	electrostatic stabiliser, hydrogen bond donor
U	LYS251	steric locator
U	MET582	polar interaction, steric role

site_id	MCSA12
Number of Residues	5
Details	M-CSA 289

Chain	Residue	Details
V	GLU139	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
V	PHE201	single electron acceptor, single electron donor, single electron relay
V	GLN202	electrostatic stabiliser, hydrogen bond donor
V	LYS251	steric locator
V	MET582	polar interaction, steric role

site_id	MCSA2
Number of Residues	5
Details	M-CSA 289

Chain	Residue	Details
B	GLU139	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
B	PHE201	single electron acceptor, single electron donor, single electron relay
B	GLN202	electrostatic stabiliser, hydrogen bond donor
B	LYS251	steric locator
B	MET582	polar interaction, steric role

site_id	MCSA3
Number of Residues	5
Details	M-CSA 289

Chain	Residue	Details
E	GLU139	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
E	PHE201	single electron acceptor, single electron donor, single electron relay
E	GLN202	electrostatic stabiliser, hydrogen bond donor
E	LYS251	steric locator
E	MET582	polar interaction, steric role

site_id	MCSA4
Number of Residues	5
Details	M-CSA 289

Chain	Residue	Details
F	GLU139	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
F	PHE201	single electron acceptor, single electron donor, single electron relay
F	GLN202	electrostatic stabiliser, hydrogen bond donor
F	LYS251	steric locator
F	MET582	polar interaction, steric role

site_id	MCSA5
Number of Residues	5
Details	M-CSA 289

Chain	Residue	Details
I	GLU139	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
I	PHE201	single electron acceptor, single electron donor, single electron relay
I	GLN202	electrostatic stabiliser, hydrogen bond donor
I	LYS251	steric locator
I	MET582	polar interaction, steric role

site_id	MCSA6
Number of Residues	5
Details	M-CSA 289

Chain	Residue	Details
J	GLU139	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
J	PHE201	single electron acceptor, single electron donor, single electron relay
J	GLN202	electrostatic stabiliser, hydrogen bond donor
J	LYS251	steric locator
J	MET582	polar interaction, steric role

site_id	MCSA7
Number of Residues	5
Details	M-CSA 289

Chain	Residue	Details
M	GLU139	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
M	PHE201	single electron acceptor, single electron donor, single electron relay
M	GLN202	electrostatic stabiliser, hydrogen bond donor
M	LYS251	steric locator
M	MET582	polar interaction, steric role

site_id	MCSA8
Number of Residues	5
Details	M-CSA 289

Chain	Residue	Details
N	GLU139	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
N	PHE201	single electron acceptor, single electron donor, single electron relay
N	GLN202	electrostatic stabiliser, hydrogen bond donor
N	LYS251	steric locator
N	MET582	polar interaction, steric role

site_id	MCSA9
Number of Residues	5
Details	M-CSA 289

Chain	Residue	Details
Q	GLU139	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
Q	PHE201	single electron acceptor, single electron donor, single electron relay
Q	GLN202	electrostatic stabiliser, hydrogen bond donor
Q	LYS251	steric locator
Q	MET582	polar interaction, steric role

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)