6T24

Cryo-EM structure of jasplakinolide-stabilized F-actin (aged)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0001725	cellular_component	stress fiber
A	0003785	molecular_function	actin monomer binding
A	0005509	molecular_function	calcium ion binding
A	0005515	molecular_function	protein binding
A	0005523	molecular_function	tropomyosin binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005856	cellular_component	cytoskeleton
A	0005865	cellular_component	striated muscle thin filament
A	0005884	cellular_component	actin filament
A	0010628	biological_process	positive regulation of gene expression
A	0016787	molecular_function	hydrolase activity
A	0019904	molecular_function	protein domain specific binding
A	0030027	cellular_component	lamellipodium
A	0030041	biological_process	actin filament polymerization
A	0030175	cellular_component	filopodium
A	0030240	biological_process	skeletal muscle thin filament assembly
A	0031013	molecular_function	troponin I binding
A	0031432	molecular_function	titin binding
A	0031941	cellular_component	filamentous actin
A	0032036	molecular_function	myosin heavy chain binding
A	0032432	cellular_component	actin filament bundle
A	0042802	molecular_function	identical protein binding
A	0044297	cellular_component	cell body
A	0048306	molecular_function	calcium-dependent protein binding
A	0048741	biological_process	skeletal muscle fiber development
A	0051017	biological_process	actin filament bundle assembly
A	0090131	biological_process	mesenchyme migration
A	0098723	cellular_component	skeletal muscle myofibril
A	0140660	molecular_function	cytoskeletal motor activator activity
B	0000166	molecular_function	nucleotide binding
B	0000287	molecular_function	magnesium ion binding
B	0001725	cellular_component	stress fiber
B	0003785	molecular_function	actin monomer binding
B	0005509	molecular_function	calcium ion binding
B	0005515	molecular_function	protein binding
B	0005523	molecular_function	tropomyosin binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005856	cellular_component	cytoskeleton
B	0005865	cellular_component	striated muscle thin filament
B	0005884	cellular_component	actin filament
B	0010628	biological_process	positive regulation of gene expression
B	0016787	molecular_function	hydrolase activity
B	0019904	molecular_function	protein domain specific binding
B	0030027	cellular_component	lamellipodium
B	0030041	biological_process	actin filament polymerization
B	0030175	cellular_component	filopodium
B	0030240	biological_process	skeletal muscle thin filament assembly
B	0031013	molecular_function	troponin I binding
B	0031432	molecular_function	titin binding
B	0031941	cellular_component	filamentous actin
B	0032036	molecular_function	myosin heavy chain binding
B	0032432	cellular_component	actin filament bundle
B	0042802	molecular_function	identical protein binding
B	0044297	cellular_component	cell body
B	0048306	molecular_function	calcium-dependent protein binding
B	0048741	biological_process	skeletal muscle fiber development
B	0051017	biological_process	actin filament bundle assembly
B	0090131	biological_process	mesenchyme migration
B	0098723	cellular_component	skeletal muscle myofibril
B	0140660	molecular_function	cytoskeletal motor activator activity
C	0000166	molecular_function	nucleotide binding
C	0000287	molecular_function	magnesium ion binding
C	0001725	cellular_component	stress fiber
C	0003785	molecular_function	actin monomer binding
C	0005509	molecular_function	calcium ion binding
C	0005515	molecular_function	protein binding
C	0005523	molecular_function	tropomyosin binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005856	cellular_component	cytoskeleton
C	0005865	cellular_component	striated muscle thin filament
C	0005884	cellular_component	actin filament
C	0010628	biological_process	positive regulation of gene expression
C	0016787	molecular_function	hydrolase activity
C	0019904	molecular_function	protein domain specific binding
C	0030027	cellular_component	lamellipodium
C	0030041	biological_process	actin filament polymerization
C	0030175	cellular_component	filopodium
C	0030240	biological_process	skeletal muscle thin filament assembly
C	0031013	molecular_function	troponin I binding
C	0031432	molecular_function	titin binding
C	0031941	cellular_component	filamentous actin
C	0032036	molecular_function	myosin heavy chain binding
C	0032432	cellular_component	actin filament bundle
C	0042802	molecular_function	identical protein binding
C	0044297	cellular_component	cell body
C	0048306	molecular_function	calcium-dependent protein binding
C	0048741	biological_process	skeletal muscle fiber development
C	0051017	biological_process	actin filament bundle assembly
C	0090131	biological_process	mesenchyme migration
C	0098723	cellular_component	skeletal muscle myofibril
C	0140660	molecular_function	cytoskeletal motor activator activity
D	0000166	molecular_function	nucleotide binding
D	0000287	molecular_function	magnesium ion binding
D	0001725	cellular_component	stress fiber
D	0003785	molecular_function	actin monomer binding
D	0005509	molecular_function	calcium ion binding
D	0005515	molecular_function	protein binding
D	0005523	molecular_function	tropomyosin binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005856	cellular_component	cytoskeleton
D	0005865	cellular_component	striated muscle thin filament
D	0005884	cellular_component	actin filament
D	0010628	biological_process	positive regulation of gene expression
D	0016787	molecular_function	hydrolase activity
D	0019904	molecular_function	protein domain specific binding
D	0030027	cellular_component	lamellipodium
D	0030041	biological_process	actin filament polymerization
D	0030175	cellular_component	filopodium
D	0030240	biological_process	skeletal muscle thin filament assembly
D	0031013	molecular_function	troponin I binding
D	0031432	molecular_function	titin binding
D	0031941	cellular_component	filamentous actin
D	0032036	molecular_function	myosin heavy chain binding
D	0032432	cellular_component	actin filament bundle
D	0042802	molecular_function	identical protein binding
D	0044297	cellular_component	cell body
D	0048306	molecular_function	calcium-dependent protein binding
D	0048741	biological_process	skeletal muscle fiber development
D	0051017	biological_process	actin filament bundle assembly
D	0090131	biological_process	mesenchyme migration
D	0098723	cellular_component	skeletal muscle myofibril
D	0140660	molecular_function	cytoskeletal motor activator activity
E	0000166	molecular_function	nucleotide binding
E	0000287	molecular_function	magnesium ion binding
E	0001725	cellular_component	stress fiber
E	0003785	molecular_function	actin monomer binding
E	0005509	molecular_function	calcium ion binding
E	0005515	molecular_function	protein binding
E	0005523	molecular_function	tropomyosin binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005856	cellular_component	cytoskeleton
E	0005865	cellular_component	striated muscle thin filament
E	0005884	cellular_component	actin filament
E	0010628	biological_process	positive regulation of gene expression
E	0016787	molecular_function	hydrolase activity
E	0019904	molecular_function	protein domain specific binding
E	0030027	cellular_component	lamellipodium
E	0030041	biological_process	actin filament polymerization
E	0030175	cellular_component	filopodium
E	0030240	biological_process	skeletal muscle thin filament assembly
E	0031013	molecular_function	troponin I binding
E	0031432	molecular_function	titin binding
E	0031941	cellular_component	filamentous actin
E	0032036	molecular_function	myosin heavy chain binding
E	0032432	cellular_component	actin filament bundle
E	0042802	molecular_function	identical protein binding
E	0044297	cellular_component	cell body
E	0048306	molecular_function	calcium-dependent protein binding
E	0048741	biological_process	skeletal muscle fiber development
E	0051017	biological_process	actin filament bundle assembly
E	0090131	biological_process	mesenchyme migration
E	0098723	cellular_component	skeletal muscle myofibril
E	0140660	molecular_function	cytoskeletal motor activator activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	15
Details	binding site for residue ADP A 376

Chain	Residue
A	GLY13
A	GLY301
A	GLY302
A	MET305
A	TYR306
A	PO4377
A	MG378
A	SER14
A	LEU16
A	LYS18
A	GLY156
A	ASP157
A	ARG210
A	LYS213
A	GLU214

---

site_id	AC2
Number of Residues	7
Details	binding site for residue PO4 A 377

Chain	Residue
A	GLY13
A	SER14
A	GLN137
A	GLY156
A	ASP157
A	ADP376
A	MG378

---

site_id	AC3
Number of Residues	3
Details	binding site for residue MG A 378

Chain	Residue
A	GLN137
A	ADP376
A	PO4377

---

site_id	AC4
Number of Residues	6
Details	binding site for residue 9ZK A 379

Chain	Residue
A	ILE75
A	LEU110
A	ALA114
A	ARG177
A	ASP179
B	ILE287

---

site_id	AC5
Number of Residues	15
Details	binding site for residue 9ZK B 379

Chain	Residue
A	ARG196
A	GLY197
A	TYR198
A	SER199
A	PHE200
A	LEU242
A	GLN246
A	VAL247
A	ILE248
B	ILE75
B	LEU110
B	ALA114
B	ARG177
B	ASP179
C	ILE287

---

site_id	AC6
Number of Residues	15
Details	binding site for residue ADP B 376

Chain	Residue
B	GLY13
B	SER14
B	LEU16
B	LYS18
B	GLY156
B	ASP157
B	ARG210
B	LYS213
B	GLU214
B	GLY301
B	GLY302
B	MET305
B	TYR306
B	PO4377
B	MG378

---

site_id	AC7
Number of Residues	7
Details	binding site for residue PO4 B 377

Chain	Residue
B	GLY13
B	SER14
B	GLN137
B	GLY156
B	ASP157
B	ADP376
B	MG378

---

site_id	AC8
Number of Residues	3
Details	binding site for residue MG B 378

Chain	Residue
B	GLN137
B	ADP376
B	PO4377

---

site_id	AC9
Number of Residues	15
Details	binding site for residue 9ZK C 379

Chain	Residue
B	ARG196
B	GLY197
B	TYR198
B	SER199
B	PHE200
B	LEU242
B	GLN246
B	VAL247
B	ILE248
C	ILE75
C	LEU110
C	ALA114
C	ARG177
C	ASP179
D	ILE287

---

site_id	AD1
Number of Residues	15
Details	binding site for residue ADP C 376

Chain	Residue
C	PO4377
C	MG378
C	GLY13
C	SER14
C	LEU16
C	LYS18
C	GLY156
C	ASP157
C	ARG210
C	LYS213
C	GLU214
C	GLY301
C	GLY302
C	MET305
C	TYR306

---

site_id	AD2
Number of Residues	7
Details	binding site for residue PO4 C 377

Chain	Residue
C	GLY13
C	SER14
C	GLN137
C	GLY156
C	ASP157
C	ADP376
C	MG378

---

site_id	AD3
Number of Residues	3
Details	binding site for residue MG C 378

Chain	Residue
C	GLN137
C	ADP376
C	PO4377

---

site_id	AD4
Number of Residues	15
Details	binding site for residue 9ZK D 379

Chain	Residue
C	ARG196
C	GLY197
C	TYR198
C	SER199
C	PHE200
C	LEU242
C	GLN246
C	VAL247
C	ILE248
D	ILE75
D	LEU110
D	ALA114
D	ARG177
D	ASP179
E	ILE287

---

site_id	AD5
Number of Residues	15
Details	binding site for residue ADP D 376

Chain	Residue
D	GLY13
D	SER14
D	LEU16
D	LYS18
D	GLY156
D	ASP157
D	ARG210
D	LYS213
D	GLU214
D	GLY301
D	GLY302
D	MET305
D	TYR306
D	PO4377
D	MG378

---

site_id	AD6
Number of Residues	7
Details	binding site for residue PO4 D 377

Chain	Residue
D	GLY13
D	SER14
D	GLN137
D	GLY156
D	ASP157
D	ADP376
D	MG378

---

site_id	AD7
Number of Residues	3
Details	binding site for residue MG D 378

Chain	Residue
D	GLN137
D	ADP376
D	PO4377

---

site_id	AD8
Number of Residues	14
Details	binding site for residue 9ZK E 379

Chain	Residue
D	ARG196
D	GLY197
D	TYR198
D	SER199
D	PHE200
D	LEU242
D	GLN246
D	VAL247
D	ILE248
E	ILE75
E	LEU110
E	ALA114
E	ARG177
E	ASP179

---

site_id	AD9
Number of Residues	15
Details	binding site for residue ADP E 376

Chain	Residue
E	GLY13
E	SER14
E	LEU16
E	LYS18
E	GLY156
E	ASP157
E	ARG210
E	LYS213
E	GLU214
E	GLY301
E	GLY302
E	MET305
E	TYR306
E	PO4377
E	MG378

---

site_id	AE1
Number of Residues	7
Details	binding site for residue PO4 E 377

Chain	Residue
E	GLY13
E	SER14
E	GLN137
E	GLY156
E	ASP157
E	ADP376
E	MG378

---

site_id	AE2
Number of Residues	3
Details	binding site for residue MG E 378

Chain	Residue
E	GLN137
E	ADP376
E	PO4377

---

Functional Information from PROSITE/UniProt

site_id	PS00406
Number of Residues	11
Details	ACTINS_1 Actins signature 1. YVGDEAQs.KRG

Chain	Residue	Details
A	TYR53-GLY63

---

site_id	PS00432
Number of Residues	9
Details	ACTINS_2 Actins signature 2. WITKqEYDE

Chain	Residue	Details
A	TRP356-GLU364

---

site_id	PS01132
Number of Residues	13
Details	ACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApLNPkaNR

Chain	Residue	Details
A	LEU104-ARG116

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	125
Details	Region: {"description":"Interaction with alpha-actinin","evidences":[{"source":"PubMed","id":"8449927","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	10
Details	Modified residue: {"description":"Methionine (R)-sulfoxide","evidences":[{"source":"UniProtKB","id":"P68134","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	5
Details	Modified residue: {"description":"N6-malonyllysine","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	5
Details	Modified residue: {"description":"Tele-methylhistidine","evidences":[{"source":"PubMed","id":"1150665","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16905096","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"213279","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"2395459","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"499690","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1ATN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1NWK","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	5
Details	Modified residue: {"description":"N6-methyllysine","evidences":[{"source":"UniProtKB","id":"P68133","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	5
Details	Modified residue: {"description":"ADP-ribosylarginine; by SpvB","evidences":[{"source":"PubMed","id":"16905096","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---