6T20
Cryo-EM structure of phalloidin-stabilized F-actin (aged)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0001725 | cellular_component | stress fiber |
| A | 0003785 | molecular_function | actin monomer binding |
| A | 0005509 | molecular_function | calcium ion binding |
| A | 0005515 | molecular_function | protein binding |
| A | 0005523 | molecular_function | tropomyosin binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005856 | cellular_component | cytoskeleton |
| A | 0005865 | cellular_component | striated muscle thin filament |
| A | 0005884 | cellular_component | actin filament |
| A | 0010628 | biological_process | positive regulation of gene expression |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0019904 | molecular_function | protein domain specific binding |
| A | 0030027 | cellular_component | lamellipodium |
| A | 0030041 | biological_process | actin filament polymerization |
| A | 0030175 | cellular_component | filopodium |
| A | 0030240 | biological_process | skeletal muscle thin filament assembly |
| A | 0031013 | molecular_function | troponin I binding |
| A | 0031432 | molecular_function | titin binding |
| A | 0031941 | cellular_component | filamentous actin |
| A | 0032036 | molecular_function | myosin heavy chain binding |
| A | 0032432 | cellular_component | actin filament bundle |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0044297 | cellular_component | cell body |
| A | 0048306 | molecular_function | calcium-dependent protein binding |
| A | 0048741 | biological_process | skeletal muscle fiber development |
| A | 0051017 | biological_process | actin filament bundle assembly |
| A | 0090131 | biological_process | mesenchyme migration |
| A | 0098723 | cellular_component | skeletal muscle myofibril |
| A | 0140660 | molecular_function | cytoskeletal motor activator activity |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0001725 | cellular_component | stress fiber |
| B | 0003785 | molecular_function | actin monomer binding |
| B | 0005509 | molecular_function | calcium ion binding |
| B | 0005515 | molecular_function | protein binding |
| B | 0005523 | molecular_function | tropomyosin binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005856 | cellular_component | cytoskeleton |
| B | 0005865 | cellular_component | striated muscle thin filament |
| B | 0005884 | cellular_component | actin filament |
| B | 0010628 | biological_process | positive regulation of gene expression |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0019904 | molecular_function | protein domain specific binding |
| B | 0030027 | cellular_component | lamellipodium |
| B | 0030041 | biological_process | actin filament polymerization |
| B | 0030175 | cellular_component | filopodium |
| B | 0030240 | biological_process | skeletal muscle thin filament assembly |
| B | 0031013 | molecular_function | troponin I binding |
| B | 0031432 | molecular_function | titin binding |
| B | 0031941 | cellular_component | filamentous actin |
| B | 0032036 | molecular_function | myosin heavy chain binding |
| B | 0032432 | cellular_component | actin filament bundle |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0044297 | cellular_component | cell body |
| B | 0048306 | molecular_function | calcium-dependent protein binding |
| B | 0048741 | biological_process | skeletal muscle fiber development |
| B | 0051017 | biological_process | actin filament bundle assembly |
| B | 0090131 | biological_process | mesenchyme migration |
| B | 0098723 | cellular_component | skeletal muscle myofibril |
| B | 0140660 | molecular_function | cytoskeletal motor activator activity |
| C | 0000287 | molecular_function | magnesium ion binding |
| C | 0001725 | cellular_component | stress fiber |
| C | 0003785 | molecular_function | actin monomer binding |
| C | 0005509 | molecular_function | calcium ion binding |
| C | 0005515 | molecular_function | protein binding |
| C | 0005523 | molecular_function | tropomyosin binding |
| C | 0005524 | molecular_function | ATP binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005856 | cellular_component | cytoskeleton |
| C | 0005865 | cellular_component | striated muscle thin filament |
| C | 0005884 | cellular_component | actin filament |
| C | 0010628 | biological_process | positive regulation of gene expression |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0019904 | molecular_function | protein domain specific binding |
| C | 0030027 | cellular_component | lamellipodium |
| C | 0030041 | biological_process | actin filament polymerization |
| C | 0030175 | cellular_component | filopodium |
| C | 0030240 | biological_process | skeletal muscle thin filament assembly |
| C | 0031013 | molecular_function | troponin I binding |
| C | 0031432 | molecular_function | titin binding |
| C | 0031941 | cellular_component | filamentous actin |
| C | 0032036 | molecular_function | myosin heavy chain binding |
| C | 0032432 | cellular_component | actin filament bundle |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0044297 | cellular_component | cell body |
| C | 0048306 | molecular_function | calcium-dependent protein binding |
| C | 0048741 | biological_process | skeletal muscle fiber development |
| C | 0051017 | biological_process | actin filament bundle assembly |
| C | 0090131 | biological_process | mesenchyme migration |
| C | 0098723 | cellular_component | skeletal muscle myofibril |
| C | 0140660 | molecular_function | cytoskeletal motor activator activity |
| D | 0000287 | molecular_function | magnesium ion binding |
| D | 0001725 | cellular_component | stress fiber |
| D | 0003785 | molecular_function | actin monomer binding |
| D | 0005509 | molecular_function | calcium ion binding |
| D | 0005515 | molecular_function | protein binding |
| D | 0005523 | molecular_function | tropomyosin binding |
| D | 0005524 | molecular_function | ATP binding |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005856 | cellular_component | cytoskeleton |
| D | 0005865 | cellular_component | striated muscle thin filament |
| D | 0005884 | cellular_component | actin filament |
| D | 0010628 | biological_process | positive regulation of gene expression |
| D | 0016787 | molecular_function | hydrolase activity |
| D | 0019904 | molecular_function | protein domain specific binding |
| D | 0030027 | cellular_component | lamellipodium |
| D | 0030041 | biological_process | actin filament polymerization |
| D | 0030175 | cellular_component | filopodium |
| D | 0030240 | biological_process | skeletal muscle thin filament assembly |
| D | 0031013 | molecular_function | troponin I binding |
| D | 0031432 | molecular_function | titin binding |
| D | 0031941 | cellular_component | filamentous actin |
| D | 0032036 | molecular_function | myosin heavy chain binding |
| D | 0032432 | cellular_component | actin filament bundle |
| D | 0042802 | molecular_function | identical protein binding |
| D | 0044297 | cellular_component | cell body |
| D | 0048306 | molecular_function | calcium-dependent protein binding |
| D | 0048741 | biological_process | skeletal muscle fiber development |
| D | 0051017 | biological_process | actin filament bundle assembly |
| D | 0090131 | biological_process | mesenchyme migration |
| D | 0098723 | cellular_component | skeletal muscle myofibril |
| D | 0140660 | molecular_function | cytoskeletal motor activator activity |
| E | 0000287 | molecular_function | magnesium ion binding |
| E | 0001725 | cellular_component | stress fiber |
| E | 0003785 | molecular_function | actin monomer binding |
| E | 0005509 | molecular_function | calcium ion binding |
| E | 0005515 | molecular_function | protein binding |
| E | 0005523 | molecular_function | tropomyosin binding |
| E | 0005524 | molecular_function | ATP binding |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0005856 | cellular_component | cytoskeleton |
| E | 0005865 | cellular_component | striated muscle thin filament |
| E | 0005884 | cellular_component | actin filament |
| E | 0010628 | biological_process | positive regulation of gene expression |
| E | 0016787 | molecular_function | hydrolase activity |
| E | 0019904 | molecular_function | protein domain specific binding |
| E | 0030027 | cellular_component | lamellipodium |
| E | 0030041 | biological_process | actin filament polymerization |
| E | 0030175 | cellular_component | filopodium |
| E | 0030240 | biological_process | skeletal muscle thin filament assembly |
| E | 0031013 | molecular_function | troponin I binding |
| E | 0031432 | molecular_function | titin binding |
| E | 0031941 | cellular_component | filamentous actin |
| E | 0032036 | molecular_function | myosin heavy chain binding |
| E | 0032432 | cellular_component | actin filament bundle |
| E | 0042802 | molecular_function | identical protein binding |
| E | 0044297 | cellular_component | cell body |
| E | 0048306 | molecular_function | calcium-dependent protein binding |
| E | 0048741 | biological_process | skeletal muscle fiber development |
| E | 0051017 | biological_process | actin filament bundle assembly |
| E | 0090131 | biological_process | mesenchyme migration |
| E | 0098723 | cellular_component | skeletal muscle myofibril |
| E | 0140660 | molecular_function | cytoskeletal motor activator activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 15 |
| Details | binding site for residue ADP A 376 |
| Chain | Residue |
| A | SER14 |
| A | GLY301 |
| A | GLY302 |
| A | MET305 |
| A | TYR306 |
| A | LYS336 |
| A | MG377 |
| A | LEU16 |
| A | LYS18 |
| A | GLY156 |
| A | ASP157 |
| A | GLY182 |
| A | ARG210 |
| A | LYS213 |
| A | GLU214 |
| site_id | AC2 |
| Number of Residues | 2 |
| Details | binding site for residue MG A 377 |
| Chain | Residue |
| A | ASP11 |
| A | ADP376 |
| site_id | AC3 |
| Number of Residues | 15 |
| Details | binding site for residue ADP B 376 |
| Chain | Residue |
| B | SER14 |
| B | LEU16 |
| B | LYS18 |
| B | GLY156 |
| B | ASP157 |
| B | GLY182 |
| B | ARG210 |
| B | LYS213 |
| B | GLU214 |
| B | GLY301 |
| B | GLY302 |
| B | MET305 |
| B | TYR306 |
| B | LYS336 |
| B | MG377 |
| site_id | AC4 |
| Number of Residues | 2 |
| Details | binding site for residue MG B 377 |
| Chain | Residue |
| B | ASP11 |
| B | ADP376 |
| site_id | AC5 |
| Number of Residues | 15 |
| Details | binding site for residue ADP C 376 |
| Chain | Residue |
| C | SER14 |
| C | LEU16 |
| C | LYS18 |
| C | GLY156 |
| C | ASP157 |
| C | GLY182 |
| C | ARG210 |
| C | LYS213 |
| C | GLU214 |
| C | GLY301 |
| C | GLY302 |
| C | MET305 |
| C | TYR306 |
| C | LYS336 |
| C | MG377 |
| site_id | AC6 |
| Number of Residues | 2 |
| Details | binding site for residue MG C 377 |
| Chain | Residue |
| C | ASP11 |
| C | ADP376 |
| site_id | AC7 |
| Number of Residues | 15 |
| Details | binding site for residue ADP D 376 |
| Chain | Residue |
| D | SER14 |
| D | LEU16 |
| D | LYS18 |
| D | GLY156 |
| D | ASP157 |
| D | GLY182 |
| D | ARG210 |
| D | LYS213 |
| D | GLU214 |
| D | GLY301 |
| D | GLY302 |
| D | MET305 |
| D | TYR306 |
| D | LYS336 |
| D | MG377 |
| site_id | AC8 |
| Number of Residues | 2 |
| Details | binding site for residue MG D 377 |
| Chain | Residue |
| D | ASP11 |
| D | ADP376 |
| site_id | AC9 |
| Number of Residues | 15 |
| Details | binding site for residue ADP E 376 |
| Chain | Residue |
| E | SER14 |
| E | LEU16 |
| E | LYS18 |
| E | GLY156 |
| E | ASP157 |
| E | GLY182 |
| E | ARG210 |
| E | LYS213 |
| E | GLU214 |
| E | GLY301 |
| E | GLY302 |
| E | MET305 |
| E | TYR306 |
| E | LYS336 |
| E | MG377 |
| site_id | AD1 |
| Number of Residues | 2 |
| Details | binding site for residue MG E 377 |
| Chain | Residue |
| E | ASP11 |
| E | ADP376 |
| site_id | AD2 |
| Number of Residues | 5 |
| Details | binding site for Di-peptide TRP F 1 and EEP F 2 |
| Chain | Residue |
| A | ILE75 |
| A | PRO112 |
| F | ALA3 |
| F | CYS5 |
| F | ALA7 |
| site_id | AD3 |
| Number of Residues | 4 |
| Details | binding site for Di-peptide EEP F 2 and ALA F 3 |
| Chain | Residue |
| F | TRP1 |
| F | DTH4 |
| F | CYS5 |
| F | ALA7 |
| site_id | AD4 |
| Number of Residues | 4 |
| Details | binding site for Di-peptide ALA F 3 and DTH F 4 |
| Chain | Residue |
| B | ARG290 |
| F | TRP1 |
| F | EEP2 |
| F | CYS5 |
| site_id | AD5 |
| Number of Residues | 5 |
| Details | binding site for Di-peptide DTH F 4 and CYS F 5 |
| Chain | Residue |
| F | TRP1 |
| F | ALA3 |
| F | HYP6 |
| F | ALA7 |
| B | ARG290 |
| site_id | AD6 |
| Number of Residues | 5 |
| Details | binding site for Di-peptide CYS F 5 and HYP F 6 |
| Chain | Residue |
| A | GLU72 |
| F | TRP1 |
| F | ALA3 |
| F | DTH4 |
| F | ALA7 |
| site_id | AD7 |
| Number of Residues | 4 |
| Details | binding site for Di-peptide HYP F 6 and ALA F 7 |
| Chain | Residue |
| A | GLU72 |
| F | TRP1 |
| F | EEP2 |
| F | CYS5 |
| site_id | AD8 |
| Number of Residues | 8 |
| Details | binding site for Di-peptide TRP G 1 and EEP G 2 |
| Chain | Residue |
| A | GLY197 |
| A | SER199 |
| A | GLN246 |
| B | ILE75 |
| B | PRO112 |
| G | ALA3 |
| G | CYS5 |
| G | ALA7 |
| site_id | AD9 |
| Number of Residues | 7 |
| Details | binding site for Di-peptide EEP G 2 and ALA G 3 |
| Chain | Residue |
| A | GLY197 |
| A | SER199 |
| A | GLN246 |
| G | TRP1 |
| G | DTH4 |
| G | CYS5 |
| G | ALA7 |
| site_id | AE1 |
| Number of Residues | 6 |
| Details | binding site for Di-peptide ALA G 3 and DTH G 4 |
| Chain | Residue |
| A | SER199 |
| A | GLU205 |
| C | ARG290 |
| G | TRP1 |
| G | EEP2 |
| G | CYS5 |
| site_id | AE2 |
| Number of Residues | 7 |
| Details | binding site for Di-peptide DTH G 4 and CYS G 5 |
| Chain | Residue |
| A | SER199 |
| A | GLU205 |
| C | ARG290 |
| G | TRP1 |
| G | ALA3 |
| G | HYP6 |
| G | ALA7 |
| site_id | AE3 |
| Number of Residues | 5 |
| Details | binding site for Di-peptide CYS G 5 and HYP G 6 |
| Chain | Residue |
| B | GLU72 |
| G | TRP1 |
| G | ALA3 |
| G | DTH4 |
| G | ALA7 |
| site_id | AE4 |
| Number of Residues | 4 |
| Details | binding site for Di-peptide HYP G 6 and ALA G 7 |
| Chain | Residue |
| B | GLU72 |
| G | TRP1 |
| G | EEP2 |
| G | CYS5 |
| site_id | AE5 |
| Number of Residues | 8 |
| Details | binding site for Di-peptide TRP H 1 and EEP H 2 |
| Chain | Residue |
| B | GLY197 |
| B | SER199 |
| B | GLN246 |
| C | ILE75 |
| C | PRO112 |
| H | ALA3 |
| H | CYS5 |
| H | ALA7 |
| site_id | AE6 |
| Number of Residues | 7 |
| Details | binding site for Di-peptide EEP H 2 and ALA H 3 |
| Chain | Residue |
| B | GLY197 |
| B | SER199 |
| B | GLN246 |
| H | TRP1 |
| H | DTH4 |
| H | CYS5 |
| H | ALA7 |
| site_id | AE7 |
| Number of Residues | 6 |
| Details | binding site for Di-peptide ALA H 3 and DTH H 4 |
| Chain | Residue |
| B | SER199 |
| B | GLU205 |
| D | ARG290 |
| H | TRP1 |
| H | EEP2 |
| H | CYS5 |
| site_id | AE8 |
| Number of Residues | 7 |
| Details | binding site for Di-peptide DTH H 4 and CYS H 5 |
| Chain | Residue |
| B | SER199 |
| B | GLU205 |
| D | ARG290 |
| H | TRP1 |
| H | ALA3 |
| H | HYP6 |
| H | ALA7 |
| site_id | AE9 |
| Number of Residues | 5 |
| Details | binding site for Di-peptide CYS H 5 and HYP H 6 |
| Chain | Residue |
| C | GLU72 |
| H | TRP1 |
| H | ALA3 |
| H | DTH4 |
| H | ALA7 |
| site_id | AF1 |
| Number of Residues | 4 |
| Details | binding site for Di-peptide HYP H 6 and ALA H 7 |
| Chain | Residue |
| C | GLU72 |
| H | TRP1 |
| H | EEP2 |
| H | CYS5 |
| site_id | AF2 |
| Number of Residues | 8 |
| Details | binding site for Di-peptide TRP I 1 and EEP I 2 |
| Chain | Residue |
| C | GLY197 |
| C | SER199 |
| C | GLN246 |
| D | ILE75 |
| D | PRO112 |
| I | ALA3 |
| I | CYS5 |
| I | ALA7 |
| site_id | AF3 |
| Number of Residues | 7 |
| Details | binding site for Di-peptide EEP I 2 and ALA I 3 |
| Chain | Residue |
| C | GLY197 |
| C | SER199 |
| C | GLN246 |
| I | TRP1 |
| I | DTH4 |
| I | CYS5 |
| I | ALA7 |
| site_id | AF4 |
| Number of Residues | 6 |
| Details | binding site for Di-peptide ALA I 3 and DTH I 4 |
| Chain | Residue |
| C | SER199 |
| C | GLU205 |
| E | ARG290 |
| I | TRP1 |
| I | EEP2 |
| I | CYS5 |
| site_id | AF5 |
| Number of Residues | 7 |
| Details | binding site for Di-peptide DTH I 4 and CYS I 5 |
| Chain | Residue |
| C | SER199 |
| C | GLU205 |
| E | ARG290 |
| I | TRP1 |
| I | ALA3 |
| I | HYP6 |
| I | ALA7 |
| site_id | AF6 |
| Number of Residues | 5 |
| Details | binding site for Di-peptide CYS I 5 and HYP I 6 |
| Chain | Residue |
| D | GLU72 |
| I | TRP1 |
| I | ALA3 |
| I | DTH4 |
| I | ALA7 |
| site_id | AF7 |
| Number of Residues | 4 |
| Details | binding site for Di-peptide HYP I 6 and ALA I 7 |
| Chain | Residue |
| D | GLU72 |
| I | TRP1 |
| I | EEP2 |
| I | CYS5 |
| site_id | AF8 |
| Number of Residues | 8 |
| Details | binding site for Di-peptide TRP J 1 and EEP J 2 |
| Chain | Residue |
| D | GLY197 |
| D | SER199 |
| D | GLN246 |
| E | ILE75 |
| E | PRO112 |
| J | ALA3 |
| J | CYS5 |
| J | ALA7 |
| site_id | AF9 |
| Number of Residues | 7 |
| Details | binding site for Di-peptide EEP J 2 and ALA J 3 |
| Chain | Residue |
| D | GLY197 |
| D | SER199 |
| D | GLN246 |
| J | TRP1 |
| J | DTH4 |
| J | CYS5 |
| J | ALA7 |
| site_id | AG1 |
| Number of Residues | 5 |
| Details | binding site for Di-peptide ALA J 3 and DTH J 4 |
| Chain | Residue |
| D | SER199 |
| D | GLU205 |
| J | TRP1 |
| J | EEP2 |
| J | CYS5 |
| site_id | AG2 |
| Number of Residues | 6 |
| Details | binding site for Di-peptide DTH J 4 and CYS J 5 |
| Chain | Residue |
| D | SER199 |
| D | GLU205 |
| J | TRP1 |
| J | ALA3 |
| J | HYP6 |
| J | ALA7 |
| site_id | AG3 |
| Number of Residues | 5 |
| Details | binding site for Di-peptide CYS J 5 and HYP J 6 |
| Chain | Residue |
| E | GLU72 |
| J | TRP1 |
| J | ALA3 |
| J | DTH4 |
| J | ALA7 |
| site_id | AG4 |
| Number of Residues | 4 |
| Details | binding site for Di-peptide HYP J 6 and ALA J 7 |
| Chain | Residue |
| E | GLU72 |
| J | TRP1 |
| J | EEP2 |
| J | CYS5 |
Functional Information from PROSITE/UniProt
| site_id | PS00406 |
| Number of Residues | 11 |
| Details | ACTINS_1 Actins signature 1. YVGDEAQs.KRG |
| Chain | Residue | Details |
| A | TYR53-GLY63 |
| site_id | PS00432 |
| Number of Residues | 9 |
| Details | ACTINS_2 Actins signature 2. WITKqEYDE |
| Chain | Residue | Details |
| A | TRP356-GLU364 |
| site_id | PS01132 |
| Number of Residues | 13 |
| Details | ACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApLNPkaNR |
| Chain | Residue | Details |
| A | LEU104-ARG116 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 5 |
| Details | MOD_RES: N-acetylaspartate; in Actin, alpha skeletal muscle => ECO:0000269|PubMed:1150665 |
| Chain | Residue | Details |
| A | ASP1 | |
| B | ASP1 | |
| C | ASP1 | |
| D | ASP1 | |
| E | ASP1 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 10 |
| Details | MOD_RES: Methionine (R)-sulfoxide => ECO:0000250|UniProtKB:P68134 |
| Chain | Residue | Details |
| A | MET44 | |
| E | MET47 | |
| A | MET47 | |
| B | MET44 | |
| B | MET47 | |
| C | MET44 | |
| C | MET47 | |
| D | MET44 | |
| D | MET47 | |
| E | MET44 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 5 |
| Details | MOD_RES: N6-malonyllysine => ECO:0000250 |
| Chain | Residue | Details |
| A | LYS61 | |
| B | LYS61 | |
| C | LYS61 | |
| D | LYS61 | |
| E | LYS61 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 5 |
| Details | MOD_RES: Tele-methylhistidine => ECO:0000269|PubMed:1150665, ECO:0000269|PubMed:16905096, ECO:0000269|PubMed:213279, ECO:0000269|PubMed:2395459, ECO:0000269|PubMed:499690, ECO:0007744|PDB:1ATN, ECO:0007744|PDB:1NWK |
| Chain | Residue | Details |
| A | HIC73 | |
| B | HIC73 | |
| C | HIC73 | |
| D | HIC73 | |
| E | HIC73 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 5 |
| Details | MOD_RES: N6-methyllysine => ECO:0000250|UniProtKB:P68133 |
| Chain | Residue | Details |
| A | LYS84 | |
| B | LYS84 | |
| C | LYS84 | |
| D | LYS84 | |
| E | LYS84 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 5 |
| Details | MOD_RES: ADP-ribosylarginine; by SpvB => ECO:0000305|PubMed:16905096 |
| Chain | Residue | Details |
| A | ARG177 | |
| B | ARG177 | |
| C | ARG177 | |
| D | ARG177 | |
| E | ARG177 |
