6SE8

Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB mutant E441Q

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
A	0004565	molecular_function	beta-galactosidase activity
A	0005975	biological_process	carbohydrate metabolic process
A	0005990	biological_process	lactose catabolic process
A	0009056	biological_process	catabolic process
A	0009341	cellular_component	beta-galactosidase complex
A	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
A	0030246	molecular_function	carbohydrate binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue ACT A 1101

Chain	Residue
A	PRO46
A	ALA47
A	ARG421
A	HOH1211
A	HOH1357
A	HOH1535

---

site_id	AC2
Number of Residues	4
Details	binding site for residue ACT A 1102

Chain	Residue
A	HOH1228
A	HIS337
A	ARG339
A	ACT1112

---

site_id	AC3
Number of Residues	6
Details	binding site for residue ACT A 1103

Chain	Residue
A	ARG346
A	HIS372
A	GLN374
A	HOH1341
A	HOH1474
A	HOH1941

---

site_id	AC4
Number of Residues	5
Details	binding site for residue ACT A 1104

Chain	Residue
A	ASP230
A	SER251
A	ARG458
A	EPE1130
A	HOH1449

---

site_id	AC5
Number of Residues	6
Details	binding site for residue ACT A 1105

Chain	Residue
A	GLU140
A	PHE142
A	PRO143
A	HIS144
A	ARG223
A	PRO224

---

site_id	AC6
Number of Residues	4
Details	binding site for residue ACT A 1106

Chain	Residue
A	PRO46
A	ASP229
A	GLY253
A	HOH1935

---

site_id	AC7
Number of Residues	6
Details	binding site for residue ACT A 1107

Chain	Residue
A	PRO654
A	ARG848
A	THR851
A	TRP852
A	PRO997
A	HOH1404

---

site_id	AC8
Number of Residues	3
Details	binding site for residue ACT A 1108

Chain	Residue
A	LEU499
A	ASN500
A	ASP501

---

site_id	AC9
Number of Residues	4
Details	binding site for residue ACT A 1109

Chain	Residue
A	HIS234
A	SER461
A	HOH1500
A	HOH1848

---

site_id	AD1
Number of Residues	2
Details	binding site for residue ACT A 1110

Chain	Residue
A	VAL288
A	HOH1560

---

site_id	AD2
Number of Residues	4
Details	binding site for residue ACT A 1111

Chain	Residue
A	GLY102
A	HIS103
A	HOH1675
A	HOH1798

---

site_id	AD3
Number of Residues	4
Details	binding site for residue ACT A 1112

Chain	Residue
A	HIS337
A	VAL348
A	ASP590
A	ACT1102

---

site_id	AD4
Number of Residues	5
Details	binding site for residue ACT A 1113

Chain	Residue
A	THR444
A	GLY445
A	LEU448
A	HIS474
A	HOH1231

---

site_id	AD5
Number of Residues	1
Details	binding site for residue ACT A 1114

Chain	Residue
A	ARG635

---

site_id	AD6
Number of Residues	2
Details	binding site for residue ACT A 1115

Chain	Residue
A	PRO884
A	ARG896

---

site_id	AD7
Number of Residues	5
Details	binding site for residue ACT A 1116

Chain	Residue
A	SER452
A	ARG453
A	LYS456
A	HIS474
A	HOH1573

---

site_id	AD8
Number of Residues	5
Details	binding site for residue NA A 1117

Chain	Residue
A	ASP207
A	PHE581
A	ASP584
A	HOH1541
A	HOH1640

---

site_id	AD9
Number of Residues	5
Details	binding site for residue NA A 1118

Chain	Residue
A	PHE536
A	TYR539
A	LEU542
A	HOH1871
A	HOH1881

---

site_id	AE1
Number of Residues	7
Details	binding site for residue NA A 1119

Chain	Residue
A	ASP651
A	THR851
A	HOH1261
A	HOH1764
A	HOH1884
A	HOH1904
A	HOH2019

---

site_id	AE2
Number of Residues	5
Details	binding site for residue NA A 1120

Chain	Residue
A	ALA44
A	HOH1292
A	HOH1728
A	HOH1799
A	HOH1833

---

site_id	AE3
Number of Residues	7
Details	binding site for residue NA A 1121

Chain	Residue
A	ARG913
A	PRO914
A	LEU954
A	ASP955
A	ALA957
A	HOH1575
A	HOH1642

---

site_id	AE4
Number of Residues	5
Details	binding site for residue NA A 1122

Chain	Residue
A	HOH1452
A	HOH1505
A	GLN882
A	GLY892
A	ALA895

---

site_id	AE5
Number of Residues	6
Details	binding site for residue MLI A 1123

Chain	Residue
A	ARG339
A	THR556
A	TYR566
A	ASP590
A	ARG592
A	HOH1460

---

site_id	AE6
Number of Residues	5
Details	binding site for residue MLI A 1124

Chain	Residue
A	HIS975
A	ARG998
A	ASP999
A	ALA1000
A	ASP1001

---

site_id	AE7
Number of Residues	6
Details	binding site for residue MLI A 1125

Chain	Residue
A	ALA10
A	HIS121
A	ARG796
A	LEU798
A	ARG815
A	HOH1337

---

site_id	AE8
Number of Residues	2
Details	binding site for residue MLI A 1126

Chain	Residue
A	ARG262
A	SER297

---

site_id	AE9
Number of Residues	7
Details	binding site for residue MLI A 1127

Chain	Residue
A	ALA313
A	ILE314
A	LYS456
A	PRO460
A	PRO463
A	ASP476
A	HOH1707

---

site_id	AF1
Number of Residues	6
Details	binding site for residue MLI A 1128

Chain	Residue
A	ALA976
A	LEU977
A	LEU995
A	ARG998
A	HOH1201
A	HOH1214

---

site_id	AF2
Number of Residues	4
Details	binding site for residue MLI A 1129

Chain	Residue
A	GLY468
A	ASP469
A	TRP470
A	HOH1658

---

site_id	AF3
Number of Residues	7
Details	binding site for residue EPE A 1130

Chain	Residue
A	ALA47
A	ASP230
A	GLY254
A	ALA256
A	ARG458
A	ACT1104
A	HOH1527

---

site_id	AF4
Number of Residues	3
Details	binding site for residue FMT A 1131

Chain	Residue
A	PHE900
A	SER901
A	HOH1446

---

site_id	AF5
Number of Residues	3
Details	binding site for residue FMT A 1132

Chain	Residue
A	LYS601
A	HIS701
A	GLU702

---

Functional Information from PROSITE/UniProt

site_id	PS00719
Number of Residues	26
Details	GLYCOSYL_HYDROL_F2_1 Glycosyl hydrolases family 2 signature 1. NaIRTSHYPphpqFLalaDqlGFYVV

Chain	Residue	Details
A	ASN362-VAL387

---