Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6S6X

Structure of Azospirillum brasilense Glutamate Synthase in a6b6 oligomeric state.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004355	molecular_function	glutamate synthase (NADPH) activity
A	0006537	biological_process	glutamate biosynthetic process
A	0006541	biological_process	glutamine metabolic process
A	0015930	molecular_function	glutamate synthase activity
A	0016491	molecular_function	oxidoreductase activity
A	0016638	molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors
A	0046872	molecular_function	metal ion binding
A	0051538	molecular_function	3 iron, 4 sulfur cluster binding
A	0097054	biological_process	L-glutamate biosynthetic process
B	0004355	molecular_function	glutamate synthase (NADPH) activity
B	0006537	biological_process	glutamate biosynthetic process
B	0006541	biological_process	glutamine metabolic process
B	0015930	molecular_function	glutamate synthase activity
B	0016491	molecular_function	oxidoreductase activity
B	0016638	molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors
B	0046872	molecular_function	metal ion binding
B	0051538	molecular_function	3 iron, 4 sulfur cluster binding
B	0097054	biological_process	L-glutamate biosynthetic process
C	0004355	molecular_function	glutamate synthase (NADPH) activity
C	0006537	biological_process	glutamate biosynthetic process
C	0006541	biological_process	glutamine metabolic process
C	0015930	molecular_function	glutamate synthase activity
C	0016491	molecular_function	oxidoreductase activity
C	0016638	molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors
C	0046872	molecular_function	metal ion binding
C	0051538	molecular_function	3 iron, 4 sulfur cluster binding
C	0097054	biological_process	L-glutamate biosynthetic process
D	0004355	molecular_function	glutamate synthase (NADPH) activity
D	0006537	biological_process	glutamate biosynthetic process
D	0006541	biological_process	glutamine metabolic process
D	0015930	molecular_function	glutamate synthase activity
D	0016491	molecular_function	oxidoreductase activity
D	0016638	molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors
D	0046872	molecular_function	metal ion binding
D	0051538	molecular_function	3 iron, 4 sulfur cluster binding
D	0097054	biological_process	L-glutamate biosynthetic process
E	0004355	molecular_function	glutamate synthase (NADPH) activity
E	0006537	biological_process	glutamate biosynthetic process
E	0006541	biological_process	glutamine metabolic process
E	0015930	molecular_function	glutamate synthase activity
E	0016491	molecular_function	oxidoreductase activity
E	0016638	molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors
E	0046872	molecular_function	metal ion binding
E	0051538	molecular_function	3 iron, 4 sulfur cluster binding
E	0097054	biological_process	L-glutamate biosynthetic process
F	0004355	molecular_function	glutamate synthase (NADPH) activity
F	0006537	biological_process	glutamate biosynthetic process
F	0006541	biological_process	glutamine metabolic process
F	0015930	molecular_function	glutamate synthase activity
F	0016491	molecular_function	oxidoreductase activity
F	0016638	molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors
F	0046872	molecular_function	metal ion binding
F	0051538	molecular_function	3 iron, 4 sulfur cluster binding
F	0097054	biological_process	L-glutamate biosynthetic process
G	0004355	molecular_function	glutamate synthase (NADPH) activity
G	0006537	biological_process	glutamate biosynthetic process
G	0016491	molecular_function	oxidoreductase activity
G	0046872	molecular_function	metal ion binding
G	0051536	molecular_function	iron-sulfur cluster binding
G	0051539	molecular_function	4 iron, 4 sulfur cluster binding
G	0097054	biological_process	L-glutamate biosynthetic process
H	0004355	molecular_function	glutamate synthase (NADPH) activity
H	0006537	biological_process	glutamate biosynthetic process
H	0016491	molecular_function	oxidoreductase activity
H	0046872	molecular_function	metal ion binding
H	0051536	molecular_function	iron-sulfur cluster binding
H	0051539	molecular_function	4 iron, 4 sulfur cluster binding
H	0097054	biological_process	L-glutamate biosynthetic process
I	0004355	molecular_function	glutamate synthase (NADPH) activity
I	0006537	biological_process	glutamate biosynthetic process
I	0016491	molecular_function	oxidoreductase activity
I	0046872	molecular_function	metal ion binding
I	0051536	molecular_function	iron-sulfur cluster binding
I	0051539	molecular_function	4 iron, 4 sulfur cluster binding
I	0097054	biological_process	L-glutamate biosynthetic process
J	0004355	molecular_function	glutamate synthase (NADPH) activity
J	0006537	biological_process	glutamate biosynthetic process
J	0016491	molecular_function	oxidoreductase activity
J	0046872	molecular_function	metal ion binding
J	0051536	molecular_function	iron-sulfur cluster binding
J	0051539	molecular_function	4 iron, 4 sulfur cluster binding
J	0097054	biological_process	L-glutamate biosynthetic process
K	0004355	molecular_function	glutamate synthase (NADPH) activity
K	0006537	biological_process	glutamate biosynthetic process
K	0016491	molecular_function	oxidoreductase activity
K	0046872	molecular_function	metal ion binding
K	0051536	molecular_function	iron-sulfur cluster binding
K	0051539	molecular_function	4 iron, 4 sulfur cluster binding
K	0097054	biological_process	L-glutamate biosynthetic process
L	0004355	molecular_function	glutamate synthase (NADPH) activity
L	0006537	biological_process	glutamate biosynthetic process
L	0016491	molecular_function	oxidoreductase activity
L	0046872	molecular_function	metal ion binding
L	0051536	molecular_function	iron-sulfur cluster binding
L	0051539	molecular_function	4 iron, 4 sulfur cluster binding
L	0097054	biological_process	L-glutamate biosynthetic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	16
Details	binding site for residue FMN A 1501

Chain	Residue
A	PRO856
A	THR1030
A	ASP1070
A	GLY1071
A	GLY1072
A	ILE1092
A	GLY1093
A	THR1094
A	GLY857
A	SER859
A	GLU886
A	GLN909
A	LYS931
A	LYS999
A	GLY1028
A	GLY1029

site_id	AC2
Number of Residues	8
Details	binding site for residue F3S A 1502

Chain	Residue
A	CYS1102
A	MET1104
A	VAL1105
A	ARG1106
A	GLN1107
A	CYS1108
A	CYS1113
A	CYS1118

site_id	AC3
Number of Residues	17
Details	binding site for residue FMN B 1501

Chain	Residue
B	PRO856
B	GLY857
B	MET858
B	SER859
B	GLU886
B	GLN909
B	LYS931
B	GLN934
B	LYS999
B	GLY1028
B	GLY1029
B	THR1030
B	GLY1071
B	GLY1072
B	ILE1092
B	GLY1093
B	THR1094

site_id	AC4
Number of Residues	8
Details	binding site for residue F3S B 1502

Chain	Residue
B	CYS1102
B	MET1104
B	VAL1105
B	ARG1106
B	GLN1107
B	CYS1108
B	CYS1113
B	CYS1118

site_id	AC5
Number of Residues	19
Details	binding site for residue FMN C 1501

Chain	Residue
C	PRO856
C	GLY857
C	MET858
C	SER859
C	GLU886
C	GLN909
C	LYS931
C	GLN934
C	LYS999
C	SER1027
C	GLY1028
C	GLY1029
C	THR1030
C	ASP1070
C	GLY1071
C	GLY1072
C	ILE1092
C	GLY1093
C	THR1094

site_id	AC6
Number of Residues	9
Details	binding site for residue F3S C 1502

Chain	Residue
C	CYS1102
C	ILE1103
C	MET1104
C	VAL1105
C	ARG1106
C	GLN1107
C	CYS1108
C	CYS1113
C	CYS1118

site_id	AC7
Number of Residues	16
Details	binding site for residue FMN D 1501

Chain	Residue
D	PRO856
D	GLY857
D	SER859
D	GLU886
D	GLN909
D	LYS931
D	LYS999
D	GLY1028
D	GLY1029
D	THR1030
D	ASP1070
D	GLY1071
D	GLY1072
D	ILE1092
D	GLY1093
D	THR1094

site_id	AC8
Number of Residues	8
Details	binding site for residue F3S D 1502

Chain	Residue
D	CYS1113
D	CYS1118
D	CYS1102
D	MET1104
D	VAL1105
D	ARG1106
D	GLN1107
D	CYS1108

site_id	AC9
Number of Residues	17
Details	binding site for residue FMN E 1501

Chain	Residue
E	PRO856
E	GLY857
E	MET858
E	SER859
E	GLU886
E	GLN909
E	LYS931
E	GLN934
E	LYS999
E	GLY1028
E	GLY1029
E	THR1030
E	GLY1071
E	GLY1072
E	ILE1092
E	GLY1093
E	THR1094

site_id	AD1
Number of Residues	8
Details	binding site for residue F3S E 1502

Chain	Residue
E	CYS1102
E	MET1104
E	VAL1105
E	ARG1106
E	GLN1107
E	CYS1108
E	CYS1113
E	CYS1118

site_id	AD2
Number of Residues	19
Details	binding site for residue FMN F 1501

Chain	Residue
F	PRO856
F	GLY857
F	MET858
F	SER859
F	GLU886
F	GLN909
F	LYS931
F	GLN934
F	LYS999
F	SER1027
F	GLY1028
F	GLY1029
F	THR1030
F	ASP1070
F	GLY1071
F	GLY1072
F	ILE1092
F	GLY1093
F	THR1094

site_id	AD3
Number of Residues	9
Details	binding site for residue F3S F 1502

Chain	Residue
F	CYS1102
F	ILE1103
F	MET1104
F	VAL1105
F	ARG1106
F	GLN1107
F	CYS1108
F	CYS1113
F	CYS1118

site_id	AD4
Number of Residues	8
Details	binding site for residue SF4 G 501

Chain	Residue
G	CYS48
G	SER49
G	CYS51
G	CYS56
G	PRO67
G	CYS109
G	VAL110
G	VAL119

site_id	AD5
Number of Residues	7
Details	binding site for residue SF4 G 502

Chain	Residue
G	CYS60
G	ASN64
G	CYS95
G	CYS99
G	GLN101
G	CYS105
G	GLU125

site_id	AD6
Number of Residues	20
Details	binding site for residue FAD G 503

Chain	Residue
G	ILE98
G	GLY157
G	PRO158
G	ALA159
G	ASP178
G	ARG179
G	GLY185
G	LEU186
G	GLU220
G	VAL221
G	THR241
G	GLY242
G	TYR244
G	ASP300
G	THR301
G	ASP304
G	PHE402
G	ASP443
G	SER449
G	LEU450

site_id	AD7
Number of Residues	7
Details	binding site for residue SF4 H 501

Chain	Residue
H	CYS48
H	SER49
H	CYS51
H	CYS56
H	PRO67
H	CYS109
H	VAL110

site_id	AD8
Number of Residues	7
Details	binding site for residue SF4 H 502

Chain	Residue
H	CYS60
H	ASN64
H	ILE66
H	CYS99
H	CYS105
H	ILE121
H	GLU125

site_id	AD9
Number of Residues	20
Details	binding site for residue FAD H 503

Chain	Residue
H	ILE98
H	GLY155
H	GLY157
H	PRO158
H	TYR177
H	ASP178
H	ARG179
H	TYR180
H	GLY185
H	LEU186
H	LYS195
H	GLU220
H	VAL221
H	THR241
H	ASP300
H	THR301
H	ASP304
H	ASP443
H	SER449
H	VAL451

site_id	AE1
Number of Residues	7
Details	binding site for residue SF4 I 501

Chain	Residue
I	CYS48
I	SER49
I	CYS51
I	CYS56
I	PRO67
I	CYS109
I	VAL110

site_id	AE2
Number of Residues	6
Details	binding site for residue SF4 I 502

Chain	Residue
I	CYS60
I	ASN64
I	ILE66
I	CYS99
I	CYS105
I	GLU125

site_id	AE3
Number of Residues	23
Details	binding site for residue FAD I 503

Chain	Residue
I	ILE98
I	GLY155
I	ALA156
I	GLY157
I	PRO158
I	ALA159
I	TYR177
I	ASP178
I	ARG179
I	TYR180
I	GLY185
I	LEU186
I	LYS195
I	GLU220
I	VAL221
I	THR241
I	TYR244
I	ASP300
I	ASP304
I	ASP443
I	SER449
I	VAL451
I	ALA454

site_id	AE4
Number of Residues	8
Details	binding site for residue SF4 J 501

Chain	Residue
J	CYS48
J	SER49
J	CYS51
J	CYS56
J	PRO67
J	CYS109
J	VAL110
J	VAL119

site_id	AE5
Number of Residues	7
Details	binding site for residue SF4 J 502

Chain	Residue
J	CYS60
J	ASN64
J	CYS95
J	CYS99
J	GLN101
J	CYS105
J	GLU125

site_id	AE6
Number of Residues	22
Details	binding site for residue FAD J 503

Chain	Residue
J	ILE98
J	ILE154
J	GLY155
J	GLY157
J	PRO158
J	ALA159
J	TYR177
J	ASP178
J	ARG179
J	TYR180
J	GLY185
J	LEU186
J	GLU220
J	VAL221
J	THR241
J	GLY242
J	TYR244
J	ASP300
J	ASP304
J	PHE402
J	ASP443
J	SER449

site_id	AE7
Number of Residues	7
Details	binding site for residue SF4 K 501

Chain	Residue
K	CYS48
K	SER49
K	CYS51
K	CYS56
K	PRO67
K	CYS109
K	VAL110

site_id	AE8
Number of Residues	7
Details	binding site for residue SF4 K 502

Chain	Residue
K	CYS60
K	ASN64
K	ILE66
K	CYS99
K	CYS105
K	ILE121
K	GLU125

site_id	AE9
Number of Residues	20
Details	binding site for residue FAD K 503

Chain	Residue
K	ILE98
K	GLY157
K	PRO158
K	ALA159
K	TYR177
K	ASP178
K	ARG179
K	TYR180
K	LEU186
K	LYS195
K	GLU220
K	VAL221
K	THR241
K	TYR244
K	ASP300
K	ASP304
K	ASP443
K	SER449
K	VAL451
K	ALA454

site_id	AF1
Number of Residues	7
Details	binding site for residue SF4 L 501

Chain	Residue
L	CYS48
L	SER49
L	CYS51
L	CYS56
L	PRO67
L	CYS109
L	VAL110

site_id	AF2
Number of Residues	6
Details	binding site for residue SF4 L 502

Chain	Residue
L	CYS60
L	ASN64
L	ILE66
L	CYS99
L	CYS105
L	GLU125

site_id	AF3
Number of Residues	20
Details	binding site for residue FAD L 503

Chain	Residue
L	ILE98
L	GLY155
L	GLY157
L	PRO158
L	TYR177
L	ASP178
L	ARG179
L	TYR180
L	GLY185
L	LEU186
L	GLU220
L	VAL221
L	THR241
L	ASP300
L	THR301
L	ASP304
L	PHE402
L	ASP443
L	SER449
L	VAL451

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	24
Details	BINDING: BINDING => ECO:0000255

Chain	Residue	Details
G	CYS95
I	CYS99
I	CYS105
I	CYS109
J	CYS95
J	CYS99
J	CYS105
J	CYS109
K	CYS95
K	CYS99
K	CYS105
G	CYS99
K	CYS109
L	CYS95
L	CYS99
L	CYS105
L	CYS109
G	CYS105
G	CYS109
H	CYS95
H	CYS99
H	CYS105
H	CYS109
I	CYS95

site_id	SWS_FT_FI2
Number of Residues	30
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
A	LEU1049
B	CYS1113
C	LEU1049
C	SER1050
C	CYS1102
C	CYS1108
C	CYS1113
D	LEU1049
D	SER1050
D	CYS1102
D	CYS1108
A	SER1050
D	CYS1113
E	LEU1049
E	SER1050
E	CYS1102
E	CYS1108
E	CYS1113
F	LEU1049
F	SER1050
F	CYS1102
F	CYS1108
A	CYS1102
F	CYS1113
A	CYS1108
A	CYS1113
B	LEU1049
B	SER1050
B	CYS1102
B	CYS1108

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 304

Chain	Residue	Details
A	CYS1	covalently attached, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
A	ASN231	electrostatic stabiliser, hydrogen bond donor
A	GLY232	electrostatic stabiliser, hydrogen bond donor
A	MET479	single electron acceptor, single electron donor, single electron relay
A	GLU886	proton acceptor, proton donor, proton relay
A	LYS937	electrostatic stabiliser

site_id	MCSA2
Number of Residues	6
Details	M-CSA 304

Chain	Residue	Details
B	CYS1	covalently attached, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
B	ASN231	electrostatic stabiliser, hydrogen bond donor
B	GLY232	electrostatic stabiliser, hydrogen bond donor
B	MET479	single electron acceptor, single electron donor, single electron relay
B	GLU886	proton acceptor, proton donor, proton relay
B	LYS937	electrostatic stabiliser

site_id	MCSA3
Number of Residues	6
Details	M-CSA 304

Chain	Residue	Details
C	CYS1	covalently attached, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
C	ASN231	electrostatic stabiliser, hydrogen bond donor
C	GLY232	electrostatic stabiliser, hydrogen bond donor
C	MET479	single electron acceptor, single electron donor, single electron relay
C	GLU886	proton acceptor, proton donor, proton relay
C	LYS937	electrostatic stabiliser

site_id	MCSA4
Number of Residues	6
Details	M-CSA 304

Chain	Residue	Details
D	CYS1	covalently attached, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
D	ASN231	electrostatic stabiliser, hydrogen bond donor
D	GLY232	electrostatic stabiliser, hydrogen bond donor
D	MET479	single electron acceptor, single electron donor, single electron relay
D	GLU886	proton acceptor, proton donor, proton relay
D	LYS937	electrostatic stabiliser

site_id	MCSA5
Number of Residues	6
Details	M-CSA 304

Chain	Residue	Details
E	CYS1	covalently attached, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
E	ASN231	electrostatic stabiliser, hydrogen bond donor
E	GLY232	electrostatic stabiliser, hydrogen bond donor
E	MET479	single electron acceptor, single electron donor, single electron relay
E	GLU886	proton acceptor, proton donor, proton relay
E	LYS937	electrostatic stabiliser

site_id	MCSA6
Number of Residues	6
Details	M-CSA 304

Chain	Residue	Details
F	CYS1	covalently attached, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
F	ASN231	electrostatic stabiliser, hydrogen bond donor
F	GLY232	electrostatic stabiliser, hydrogen bond donor
F	MET479	single electron acceptor, single electron donor, single electron relay
F	GLU886	proton acceptor, proton donor, proton relay
F	LYS937	electrostatic stabiliser

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)