6S2A
PI PLC mutant H82A
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004436 | molecular_function | phosphatidylinositol diacylglycerol-lyase activity |
| A | 0005576 | cellular_component | extracellular region |
| A | 0006629 | biological_process | lipid metabolic process |
| A | 0008081 | molecular_function | phosphoric diester hydrolase activity |
| A | 0016042 | biological_process | lipid catabolic process |
| A | 0016829 | molecular_function | lyase activity |
| B | 0004436 | molecular_function | phosphatidylinositol diacylglycerol-lyase activity |
| B | 0005576 | cellular_component | extracellular region |
| B | 0006629 | biological_process | lipid metabolic process |
| B | 0008081 | molecular_function | phosphoric diester hydrolase activity |
| B | 0016042 | biological_process | lipid catabolic process |
| B | 0016829 | molecular_function | lyase activity |
| C | 0004436 | molecular_function | phosphatidylinositol diacylglycerol-lyase activity |
| C | 0005576 | cellular_component | extracellular region |
| C | 0006629 | biological_process | lipid metabolic process |
| C | 0008081 | molecular_function | phosphoric diester hydrolase activity |
| C | 0016042 | biological_process | lipid catabolic process |
| C | 0016829 | molecular_function | lyase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 9 |
| Details | binding site for residue INS A 301 |
| Chain | Residue |
| A | HIS32 |
| A | ARG69 |
| A | LYS115 |
| A | ARG163 |
| A | ASP180 |
| A | ASP198 |
| A | TYR200 |
| A | HOH444 |
| A | HOH449 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | binding site for residue INS B 301 |
| Chain | Residue |
| B | HIS32 |
| B | ARG69 |
| B | LYS115 |
| B | ARG163 |
| B | ASP198 |
| B | TYR200 |
| B | HOH414 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | binding site for residue INS C 301 |
| Chain | Residue |
| C | HIS32 |
| C | ARG69 |
| C | LYS115 |
| C | ARG163 |
| C | ASP198 |
| C | HOH402 |
| C | HOH412 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 423 |
| Details | Domain: {"description":"PI-PLC X-box","evidences":[{"source":"PROSITE-ProRule","id":"PRU00270","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 3 |
| Details | Active site: {"description":"Proton acceptor"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 3 |
| Details | Active site: {"description":"Proton donor"} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 5 |
| Details | M-CSA 26 |
| Chain | Residue | Details |
| A | HIS32 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| A | ASP33 | electrostatic stabiliser, hydrogen bond acceptor |
| A | ARG69 | electrostatic stabiliser, hydrogen bond donor |
| A | ALA82 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| A | ASP274 | electrostatic stabiliser, hydrogen bond acceptor |
| site_id | MCSA2 |
| Number of Residues | 5 |
| Details | M-CSA 26 |
| Chain | Residue | Details |
| B | HIS32 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| B | ASP33 | electrostatic stabiliser, hydrogen bond acceptor |
| B | ARG69 | electrostatic stabiliser, hydrogen bond donor |
| B | ALA82 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| B | ASP274 | electrostatic stabiliser, hydrogen bond acceptor |
| site_id | MCSA3 |
| Number of Residues | 5 |
| Details | M-CSA 26 |
| Chain | Residue | Details |
| C | HIS32 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| C | ASP33 | electrostatic stabiliser, hydrogen bond acceptor |
| C | ARG69 | electrostatic stabiliser, hydrogen bond donor |
| C | ALA82 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| C | ASP274 | electrostatic stabiliser, hydrogen bond acceptor |
