Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6RP2

Threonine to Cysteine (T225C) variant of E coli hydrogenase-1

Functional Information from GO Data

Chain	GOid	namespace	contents
L	0005515	molecular_function	protein binding
L	0005886	cellular_component	plasma membrane
L	0006113	biological_process	fermentation
L	0008901	molecular_function	ferredoxin hydrogenase activity
L	0009055	molecular_function	electron transfer activity
L	0009061	biological_process	anaerobic respiration
L	0009267	biological_process	cellular response to starvation
L	0016020	cellular_component	membrane
L	0016151	molecular_function	nickel cation binding
L	0016491	molecular_function	oxidoreductase activity
L	0019645	biological_process	anaerobic electron transport chain
L	0030288	cellular_component	outer membrane-bounded periplasmic space
L	0033748	molecular_function	hydrogenase (acceptor) activity
L	0044569	cellular_component	[Ni-Fe] hydrogenase complex
L	0046872	molecular_function	metal ion binding
L	0098567	cellular_component	periplasmic side of plasma membrane
L	1902421	biological_process	hydrogen metabolic process
M	0005515	molecular_function	protein binding
M	0005886	cellular_component	plasma membrane
M	0006113	biological_process	fermentation
M	0008901	molecular_function	ferredoxin hydrogenase activity
M	0009055	molecular_function	electron transfer activity
M	0009061	biological_process	anaerobic respiration
M	0009267	biological_process	cellular response to starvation
M	0016020	cellular_component	membrane
M	0016151	molecular_function	nickel cation binding
M	0016491	molecular_function	oxidoreductase activity
M	0019645	biological_process	anaerobic electron transport chain
M	0030288	cellular_component	outer membrane-bounded periplasmic space
M	0033748	molecular_function	hydrogenase (acceptor) activity
M	0044569	cellular_component	[Ni-Fe] hydrogenase complex
M	0046872	molecular_function	metal ion binding
M	0098567	cellular_component	periplasmic side of plasma membrane
M	1902421	biological_process	hydrogen metabolic process
S	0005515	molecular_function	protein binding
S	0005886	cellular_component	plasma membrane
S	0006113	biological_process	fermentation
S	0008901	molecular_function	ferredoxin hydrogenase activity
S	0009055	molecular_function	electron transfer activity
S	0009061	biological_process	anaerobic respiration
S	0009267	biological_process	cellular response to starvation
S	0009375	cellular_component	ferredoxin hydrogenase complex
S	0016020	cellular_component	membrane
S	0016491	molecular_function	oxidoreductase activity
S	0019645	biological_process	anaerobic electron transport chain
S	0033748	molecular_function	hydrogenase (acceptor) activity
S	0044569	cellular_component	[Ni-Fe] hydrogenase complex
S	0046872	molecular_function	metal ion binding
S	0051536	molecular_function	iron-sulfur cluster binding
S	0051538	molecular_function	3 iron, 4 sulfur cluster binding
S	0051539	molecular_function	4 iron, 4 sulfur cluster binding
S	0098567	cellular_component	periplasmic side of plasma membrane
S	1902421	biological_process	hydrogen metabolic process
T	0005515	molecular_function	protein binding
T	0005886	cellular_component	plasma membrane
T	0006113	biological_process	fermentation
T	0008901	molecular_function	ferredoxin hydrogenase activity
T	0009055	molecular_function	electron transfer activity
T	0009061	biological_process	anaerobic respiration
T	0009267	biological_process	cellular response to starvation
T	0009375	cellular_component	ferredoxin hydrogenase complex
T	0016020	cellular_component	membrane
T	0016491	molecular_function	oxidoreductase activity
T	0019645	biological_process	anaerobic electron transport chain
T	0033748	molecular_function	hydrogenase (acceptor) activity
T	0044569	cellular_component	[Ni-Fe] hydrogenase complex
T	0046872	molecular_function	metal ion binding
T	0051536	molecular_function	iron-sulfur cluster binding
T	0051538	molecular_function	3 iron, 4 sulfur cluster binding
T	0051539	molecular_function	4 iron, 4 sulfur cluster binding
T	0098567	cellular_component	periplasmic side of plasma membrane
T	1902421	biological_process	hydrogen metabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue SF4 S 401

Chain	Residue
S	HIS187
S	CYS190
S	ARG193
S	CYS215
S	LEU216
S	CYS221

site_id	AC2
Number of Residues	8
Details	binding site for residue F3S S 402

Chain	Residue
S	TRP235
S	PRO242
S	CYS249
S	LEU250
S	CYS252
L	LYS226
S	ASN228
S	CYS230

site_id	AC3
Number of Residues	9
Details	binding site for residue SF3 S 403

Chain	Residue
S	CYS17
S	THR18
S	CYS19
S	CYS20
S	GLU76
S	THR114
S	CYS115
S	CYS120
S	CYS149

site_id	AC4
Number of Residues	9
Details	binding site for residue LMT S 404

Chain	Residue
S	ILE7
S	TRP11
S	SER107
S	ALA108
S	MET163
S	ARG168
S	HOH599
S	HOH627
S	HOH630

site_id	AC5
Number of Residues	5
Details	binding site for residue CL S 405

Chain	Residue
S	THR114
S	TRP118
S	CYS120
S	GLY256
S	HOH609

site_id	AC6
Number of Residues	5
Details	binding site for residue SO4 L 601

Chain	Residue
L	LEU482
L	ALA483
L	HOH712
L	HOH714
S	ARG211

site_id	AC7
Number of Residues	13
Details	binding site for residue FCO L 602

Chain	Residue
L	CSO79
L	VAL82
L	HIS83
L	ALA507
L	PRO508
L	ARG509
L	LEU512
L	VAL530
L	PRO531
L	THR532
L	CYS579
L	NI603
L	HOH1097

site_id	AC8
Number of Residues	6
Details	binding site for residue NI L 603

Chain	Residue
L	CYS76
L	CSO79
L	CYS576
L	CYS579
L	FCO602
L	HOH1097

site_id	AC9
Number of Residues	6
Details	binding site for residue MG L 604

Chain	Residue
L	GLU57
L	CYS528
L	HIS582
L	HOH724
L	HOH729
L	HOH733

site_id	AD1
Number of Residues	4
Details	binding site for residue LI L 605

Chain	Residue
L	HOH854
L	HOH873
L	HOH1023
L	HOH1145

site_id	AD2
Number of Residues	6
Details	binding site for residue SF4 T 401

Chain	Residue
T	HIS187
T	CYS190
T	ARG193
T	CYS215
T	LEU216
T	CYS221

site_id	AD3
Number of Residues	9
Details	binding site for residue F3S T 402

Chain	Residue
M	LYS226
T	THR226
T	ASN228
T	CYS230
T	TRP235
T	PRO242
T	CYS249
T	LEU250
T	CYS252

site_id	AD4
Number of Residues	9
Details	binding site for residue SF3 T 403

Chain	Residue
T	CYS17
T	THR18
T	CYS19
T	CYS20
T	GLU76
T	THR114
T	CYS115
T	CYS120
T	CYS149

site_id	AD5
Number of Residues	4
Details	binding site for residue LMT T 404

Chain	Residue
T	ILE7
T	TRP11
T	MET163
T	VAL164

site_id	AD6
Number of Residues	4
Details	binding site for residue CL T 405

Chain	Residue
T	TRP118
T	CYS120
T	GLY256
T	HOH635

site_id	AD7
Number of Residues	13
Details	binding site for residue FCO M 601

Chain	Residue
M	CSO79
M	VAL82
M	HIS83
M	ALA507
M	PRO508
M	ARG509
M	LEU512
M	VAL530
M	PRO531
M	THR532
M	CYS579
M	NI602
M	HOH1022

site_id	AD8
Number of Residues	6
Details	binding site for residue NI M 602

Chain	Residue
M	CYS76
M	CSO79
M	CYS576
M	CYS579
M	FCO601
M	HOH1022

site_id	AD9
Number of Residues	6
Details	binding site for residue MG M 603

Chain	Residue
M	GLU57
M	CYS528
M	HIS582
M	HOH730
M	HOH736
M	HOH742

site_id	AE1
Number of Residues	3
Details	binding site for residue SO4 M 604

Chain	Residue
M	ARG394
M	HOH704
S	ARG125

site_id	AE2
Number of Residues	4
Details	binding site for residue LI M 605

Chain	Residue
M	HOH837
M	HOH909
M	HOH954
M	HOH1155

Functional Information from PROSITE/UniProt

site_id	PS00507
Number of Residues	26
Details	NI_HGENASE_L_1 Nickel-dependent hydrogenases large subunit signature 1. RGLEiilqgrdprdawafvERiCGVC

Chain	Residue	Details
L	ARG54-CSO79

site_id	PS00508
Number of Residues	10
Details	NI_HGENASE_L_2 Nickel-dependent hydrogenases large subunit signature 2. FDPCLACst.H

Chain	Residue	Details
L	PHE573-HIS582

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	BINDING: BINDING => ECO:0000255

Chain	Residue	Details
L	CYS76
L	CSO79
L	CYS576
L	CYS579
M	CYS76
M	CSO79
M	CYS576
M	CYS579

site_id	SWS_FT_FI2
Number of Residues	94
Details	TOPO_DOM: Cytoplasmic => ECO:0000255

Chain	Residue	Details
S	VAL303-ALA327
T	VAL303-ALA327
S	ASP304-ALA327
T	ASP304-ALA327

site_id	SWS_FT_FI3
Number of Residues	22
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P21853

Chain	Residue	Details
S	CYS17
S	CYS249
S	CYS252
T	CYS17
T	CYS20
T	CYS115
T	CYS149
T	HIS187
T	CYS190
T	CYS215
T	CYS221
S	CYS20
T	CYS230
T	CYS249
T	CYS252
S	CYS115
S	CYS149
S	HIS187
S	CYS190
S	CYS215
S	CYS221
S	CYS230

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)